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The map label for this gene is ilvE [H]

Identifier: 213692445

GI number: 213692445

Start: 1723918

End: 1725045

Strand: Reverse

Name: ilvE [H]

Synonym: Blon_1574

Alternate gene names: 213692445

Gene position: 1725045-1723918 (Counterclockwise)

Preceding gene: 213692446

Following gene: 213692444

Centisome position: 60.9

GC content: 62.59

Gene sequence:

>1128_bases
ATGACTGAAAATACGCACCACGATCCGGCAGCGCTCGACCAGCTCACCGAACCGTTCACCGTACTGCCGAACGACAACCC
CGCATCCGATGAGAAGCGTCAGTCCCTCATCGACAAGCCGGCTTTCGGCCAGGTGTTCTCTGACAACATGACTCACATGA
CTTGGACCAAAGGCGAAGGCTGGTCCGACCGCCGCGTCGAGCCGTACGCGCCGCTCAAGATGGACCCGGGTGCCTCTGTG
CTGCACTATGCACAGGAGTGTTTCGAGGGTCTGAAGGCTTATCGTCACGCTGACGGCTCCACCTGGCTGTTCCGCCCGGA
TGCGAACGCCGAGCGTTTCCAGAATTCCGCCAAGCGTCTATACCTGCCCGAGCTGCCGGTTGACGACTTCCTCGGCTCCG
TGGCCGCGCTGGTCAAGCGCGACGCCAACTGGGTGCCGTCACGCCGTGAGTACACACTGTACATGCGTCCGTTCATGTTC
GCCTCCGAGCCGTTCCTCGGCGTGCGCGCTCCGCAGGAAGTCGACTACTGCGTGATCGCCTCTCCGTCCGGCCCGTACTT
CCCGGGCGGCGTCAAGCCGGTGAGCATCTGGGTTGAAGACAAGTGGTTCCGCACCGGCCCCGGCGGCACTGGTTTCGCCA
AGTGCGGTGGCAACTACGCTGCCTCCCTGCTCGGTGAGTATAAGGGTATCGAGAACGGCTGCGAGCAGGTCTGCTTTGTG
GACGCCGCCACCAAGACCTACCTTGAAGAGCTTGGCGGCATGAACATGATGGCCGTGCACAAGGATGGCCACGTGGAGAC
CCCGTCCCTGACCGGCAACATTTTGCCGGGCGTGACCCGTCGCTCCCTGATTCAGCTGCTTCAGGACAAGGGCCACGACG
TGGTCGAGACCATGATCGCCCTCGACCAGCTGCTCGAGGACATTAAGTCCGGCGAGGTCACTGAGGTGTTCGCTTGCGGT
ACCGCCGCCATCATTACCCCGATCGGTCGCTTCAAGTCCGAGAAGTTCGATGTCACCGTCGCCGACGGTGGCTCCGGCGA
GCTGACCGTCGCCCTGCGCAACGAGTTGCTCGGCATCCAGCTCGGCGAGGTGGAAGACCCGCACAACTGGATGTGGAAGG
TGTGCTGA

Upstream 100 bases:

>100_bases
GGTGTGCAAAAGTATGCAACAGAGCTCGCGCCACAAGCGCACAGCACACACCCCATATGGTGGCTTTCGGGCTGCCAAAG
TCAAGAAGAAGTAGCTAGTA

Downstream 100 bases:

>100_bases
AAAGCCCGCTGATTAGGCATTGAGACCCCTTACGAACGACAATGTTTGCAAGGGGTCTCATCGTATCTCGGGTAAGATTT
CCGGCGTCTAGGAATGTCAT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTENTHHDPAALDQLTEPFTVLPNDNPASDEKRQSLIDKPAFGQVFSDNMTHMTWTKGEGWSDRRVEPYAPLKMDPGASV
LHYAQECFEGLKAYRHADGSTWLFRPDANAERFQNSAKRLYLPELPVDDFLGSVAALVKRDANWVPSRREYTLYMRPFMF
ASEPFLGVRAPQEVDYCVIASPSGPYFPGGVKPVSIWVEDKWFRTGPGGTGFAKCGGNYAASLLGEYKGIENGCEQVCFV
DAATKTYLEELGGMNMMAVHKDGHVETPSLTGNILPGVTRRSLIQLLQDKGHDVVETMIALDQLLEDIKSGEVTEVFACG
TAAIITPIGRFKSEKFDVTVADGGSGELTVALRNELLGIQLGEVEDPHNWMWKVC

Sequences:

>Translated_375_residues
MTENTHHDPAALDQLTEPFTVLPNDNPASDEKRQSLIDKPAFGQVFSDNMTHMTWTKGEGWSDRRVEPYAPLKMDPGASV
LHYAQECFEGLKAYRHADGSTWLFRPDANAERFQNSAKRLYLPELPVDDFLGSVAALVKRDANWVPSRREYTLYMRPFMF
ASEPFLGVRAPQEVDYCVIASPSGPYFPGGVKPVSIWVEDKWFRTGPGGTGFAKCGGNYAASLLGEYKGIENGCEQVCFV
DAATKTYLEELGGMNMMAVHKDGHVETPSLTGNILPGVTRRSLIQLLQDKGHDVVETMIALDQLLEDIKSGEVTEVFACG
TAAIITPIGRFKSEKFDVTVADGGSGELTVALRNELLGIQLGEVEDPHNWMWKVC
>Mature_374_residues
TENTHHDPAALDQLTEPFTVLPNDNPASDEKRQSLIDKPAFGQVFSDNMTHMTWTKGEGWSDRRVEPYAPLKMDPGASVL
HYAQECFEGLKAYRHADGSTWLFRPDANAERFQNSAKRLYLPELPVDDFLGSVAALVKRDANWVPSRREYTLYMRPFMFA
SEPFLGVRAPQEVDYCVIASPSGPYFPGGVKPVSIWVEDKWFRTGPGGTGFAKCGGNYAASLLGEYKGIENGCEQVCFVD
AATKTYLEELGGMNMMAVHKDGHVETPSLTGNILPGVTRRSLIQLLQDKGHDVVETMIALDQLLEDIKSGEVTEVFACGT
AAIITPIGRFKSEKFDVTVADGGSGELTVALRNELLGIQLGEVEDPHNWMWKVC

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=348, Percent_Identity=37.0689655172414, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI296010904, Length=348, Percent_Identity=37.0689655172414, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI38176287, Length=348, Percent_Identity=37.0689655172414, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI50658084, Length=342, Percent_Identity=38.3040935672515, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI258614015, Length=297, Percent_Identity=38.7205387205387, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI296010902, Length=348, Percent_Identity=32.183908045977, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI296010900, Length=348, Percent_Identity=32.183908045977, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI48994963, Length=329, Percent_Identity=29.7872340425532, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17568601, Length=299, Percent_Identity=35.1170568561873, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17565728, Length=350, Percent_Identity=31.7142857142857, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322002, Length=359, Percent_Identity=35.3760445682451, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322608, Length=342, Percent_Identity=35.0877192982456, Blast_Score=202, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24641779, Length=334, Percent_Identity=34.4311377245509, Blast_Score=185, Evalue=5e-47,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 41473; Mature: 41342

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENTHHDPAALDQLTEPFTVLPNDNPASDEKRQSLIDKPAFGQVFSDNMTHMTWTKGEG
CCCCCCCCHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCCCHHHCCCCCEEEEECCCC
WSDRRVEPYAPLKMDPGASVLHYAQECFEGLKAYRHADGSTWLFRPDANAERFQNSAKRL
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCEE
YLPELPVDDFLGSVAALVKRDANWVPSRREYTLYMRPFMFASEPFLGVRAPQEVDYCVIA
ECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCCEEEEE
SPSGPYFPGGVKPVSIWVEDKWFRTGPGGTGFAKCGGNYAASLLGEYKGIENGCEQVCFV
CCCCCCCCCCCCEEEEEEECCEEECCCCCCCHHHCCCHHHHHHHHHHCCHHHHHHHEEEE
DAATKTYLEELGGMNMMAVHKDGHVETPSLTGNILPGVTRRSLIQLLQDKGHDVVETMIA
HHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LDQLLEDIKSGEVTEVFACGTAAIITPIGRFKSEKFDVTVADGGSGELTVALRNELLGIQ
HHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCEEE
LGEVEDPHNWMWKVC
ECCCCCCCCCEEECC
>Mature Secondary Structure 
TENTHHDPAALDQLTEPFTVLPNDNPASDEKRQSLIDKPAFGQVFSDNMTHMTWTKGEG
CCCCCCCHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCCCHHHCCCCCEEEEECCCC
WSDRRVEPYAPLKMDPGASVLHYAQECFEGLKAYRHADGSTWLFRPDANAERFQNSAKRL
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCEE
YLPELPVDDFLGSVAALVKRDANWVPSRREYTLYMRPFMFASEPFLGVRAPQEVDYCVIA
ECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCCEEEEE
SPSGPYFPGGVKPVSIWVEDKWFRTGPGGTGFAKCGGNYAASLLGEYKGIENGCEQVCFV
CCCCCCCCCCCCEEEEEEECCEEECCCCCCCHHHCCCHHHHHHHHHHCCHHHHHHHEEEE
DAATKTYLEELGGMNMMAVHKDGHVETPSLTGNILPGVTRRSLIQLLQDKGHDVVETMIA
HHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LDQLLEDIKSGEVTEVFACGTAAIITPIGRFKSEKFDVTVADGGSGELTVALRNELLGIQ
HHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCEEE
LGEVEDPHNWMWKVC
ECCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]