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The map label for this gene is lepA [H]

Identifier: 213692359

GI number: 213692359

Start: 1655876

End: 1657756

Strand: Reverse

Name: lepA [H]

Synonym: Blon_1486

Alternate gene names: 213692359

Gene position: 1657756-1655876 (Counterclockwise)

Preceding gene: 213692361

Following gene: 213692358

Centisome position: 58.52

GC content: 60.18

Gene sequence:

>1881_bases
TTGGTCGTCCAACACAATCAGCCGGGTTCCACTGATCAGTCGGTGATTCGCAACTTCTGTATCATCGCACACATCGACCA
CGGCAAGTCGACCGTGGCCGACCGCATCCTGCAGTTGAGCGGCATTGTGCCCGAGCGAGAGATGCGCGATCGCTTCCTTG
ACCGTATGGATATCGAGCAGGAGCGTGGCATCACCATCAAATCCCAGGCCGTGCGCGTGCCATGGACCTTTGAGGGTACC
GAATACACGCTCGGCATGATCGACACCCCCGGCCACGTGGACTTCACCTATGAGGTGTCCCGCGCACTGGCCGCCTGCGA
AGGCGCGGTGCTGCTCGTCGACGCTACCCAGGGCATCGAAGCGCAGACCCTGTCTAACCTGTACATGGCCATCGACCATG
ATCTGGCCATCATTCCCGTGCTCAACAAAATCGACCTGCCGTCCGCAGAACCGGACAAGCATGCCGAGGAGATCGCAGGT
CTGATCGGCTGCGAGCCGAGCGACGTGCTGCGCGTCTCCGGCAAAACCGGCGAAGGTGTGGCCGACCTGCTCGACCAGAT
CGTCATGGACGTGCCCGCCCCGCACGGCGACCCCGATGCCCCCGCCCGCGCGCTGATCTTCGACTCTGTCTACGACTCCT
ACCGCGGCATCGTCACCTACATCCGTATGGAGGACGGCGAACTGCACGACCGCGAAAAAGTGCACATGATGGGTATCGGC
ATGACCCATGATCCCATCGAGATCGGTGTGATCAGTCCGGACATGACTCGCACCAAAGCGCTGGGTGCCGGCGAGGTCGG
CTACATCATCACCGGTGCGAAAGATGTGAGTCAGTCCAAGGTGGGCGACACCCTGACCTCGGCCGTCCGCCCGGCCACCG
AGCCGCTGCCTGGCTACCGCGACCCCAAGCCGATGGTCTACGCCGGTTTGTTCCCGATCGACAACGCGCAATTCCCCGAA
CTGCGTGATGCACTCGACAAACTCAAGCTCAACGACGCCGCCCTGATCTACACGCCGGAAACCTCCGTGGCTCTGGGTTT
CGGCTTCCGCTGTGGCTTCCTGGGTCTGCTGCACATGGAAATCGTCAACGAGCGACTCTCCCGCGAGTTCGGCCTCGACC
TTATCCAGACCGCCCCGAACGTCACCTACGACGTGACCGCCGAGGATGGCAGCCAGCATCATGTGACCAACCCGAGCGAA
TTCCCGGACGGCAAGATCAAGAAGATCGTCGAGCCGATGGTTGCCGCCGACATCATCACGCCCAAGGAATTCATCGGCGC
GGTGATGGATCTGTGCCAGGACCACCGCGGCATTATGGGCACGATGGAGTACATCTCCACCGACCGTGTGGAGATGCACT
ACCGCATTCCACTGGCCGAAATCGTGTTCGACTTCTTCGATCAGCTCAAGTCCCGTACCAAGGGCTACGCCTCCCTTGAT
TACCACGAGGACGGCGAGCAGTCCGCCGATTTGGTCAAGGTGGACATCCTTATTCAGGGCGAGAAGGTCGATGCGTTCAG
TGCCATCGTTCACCGCGATAAGGCGTATTCGTACGGTGTGATGATGACCAAGAAATTGCGTAGTCTGATTCCTCGCCAGC
AGTTTGAGATTCCGATTCAGGCTGCGATTGGTTCGCGCATCATCGCGCGTGAGAACATTCGTGCGTTACGCAAGGACGTG
CTCGCCAAATGCTATGGCGGCGACATCACCCGTAAGCGCAAGTTGCTCGAAAAGCAGAAGGCTGGCAAGAAGCGTATGAA
GATGCTCGGCCATGTAGAGGTGCCGCAGGAGGCGTTCATCGCCGCCCTGTCCACCGGCGAGGATTCCAACGATCGCGACA
CCAAAGACAAGATTCGCGCCGCGCAGAAGACGGAAGGCTGA

Upstream 100 bases:

>100_bases
GATAGCATGAGCCCCGGAAAAACCCGCGCAATGCCACGCCGAATACAGTCCCTCGGCTACACTAGGTGAAGTTTGAGTAT
ATGCCAAGAGGAGGAATGCG

Downstream 100 bases:

>100_bases
GCGGGTGCCGGAGGCTCGCCTCGCCGGGGAGTCTTCGGCACGGATGGCTGAGGGGAGTGACGGTTGACTTACGAGATCTA
CATTCATGTGCCGTTCTGTC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MVVQHNQPGSTDQSVIRNFCIIAHIDHGKSTVADRILQLSGIVPEREMRDRFLDRMDIEQERGITIKSQAVRVPWTFEGT
EYTLGMIDTPGHVDFTYEVSRALAACEGAVLLVDATQGIEAQTLSNLYMAIDHDLAIIPVLNKIDLPSAEPDKHAEEIAG
LIGCEPSDVLRVSGKTGEGVADLLDQIVMDVPAPHGDPDAPARALIFDSVYDSYRGIVTYIRMEDGELHDREKVHMMGIG
MTHDPIEIGVISPDMTRTKALGAGEVGYIITGAKDVSQSKVGDTLTSAVRPATEPLPGYRDPKPMVYAGLFPIDNAQFPE
LRDALDKLKLNDAALIYTPETSVALGFGFRCGFLGLLHMEIVNERLSREFGLDLIQTAPNVTYDVTAEDGSQHHVTNPSE
FPDGKIKKIVEPMVAADIITPKEFIGAVMDLCQDHRGIMGTMEYISTDRVEMHYRIPLAEIVFDFFDQLKSRTKGYASLD
YHEDGEQSADLVKVDILIQGEKVDAFSAIVHRDKAYSYGVMMTKKLRSLIPRQQFEIPIQAAIGSRIIARENIRALRKDV
LAKCYGGDITRKRKLLEKQKAGKKRMKMLGHVEVPQEAFIAALSTGEDSNDRDTKDKIRAAQKTEG

Sequences:

>Translated_626_residues
MVVQHNQPGSTDQSVIRNFCIIAHIDHGKSTVADRILQLSGIVPEREMRDRFLDRMDIEQERGITIKSQAVRVPWTFEGT
EYTLGMIDTPGHVDFTYEVSRALAACEGAVLLVDATQGIEAQTLSNLYMAIDHDLAIIPVLNKIDLPSAEPDKHAEEIAG
LIGCEPSDVLRVSGKTGEGVADLLDQIVMDVPAPHGDPDAPARALIFDSVYDSYRGIVTYIRMEDGELHDREKVHMMGIG
MTHDPIEIGVISPDMTRTKALGAGEVGYIITGAKDVSQSKVGDTLTSAVRPATEPLPGYRDPKPMVYAGLFPIDNAQFPE
LRDALDKLKLNDAALIYTPETSVALGFGFRCGFLGLLHMEIVNERLSREFGLDLIQTAPNVTYDVTAEDGSQHHVTNPSE
FPDGKIKKIVEPMVAADIITPKEFIGAVMDLCQDHRGIMGTMEYISTDRVEMHYRIPLAEIVFDFFDQLKSRTKGYASLD
YHEDGEQSADLVKVDILIQGEKVDAFSAIVHRDKAYSYGVMMTKKLRSLIPRQQFEIPIQAAIGSRIIARENIRALRKDV
LAKCYGGDITRKRKLLEKQKAGKKRMKMLGHVEVPQEAFIAALSTGEDSNDRDTKDKIRAAQKTEG
>Mature_626_residues
MVVQHNQPGSTDQSVIRNFCIIAHIDHGKSTVADRILQLSGIVPEREMRDRFLDRMDIEQERGITIKSQAVRVPWTFEGT
EYTLGMIDTPGHVDFTYEVSRALAACEGAVLLVDATQGIEAQTLSNLYMAIDHDLAIIPVLNKIDLPSAEPDKHAEEIAG
LIGCEPSDVLRVSGKTGEGVADLLDQIVMDVPAPHGDPDAPARALIFDSVYDSYRGIVTYIRMEDGELHDREKVHMMGIG
MTHDPIEIGVISPDMTRTKALGAGEVGYIITGAKDVSQSKVGDTLTSAVRPATEPLPGYRDPKPMVYAGLFPIDNAQFPE
LRDALDKLKLNDAALIYTPETSVALGFGFRCGFLGLLHMEIVNERLSREFGLDLIQTAPNVTYDVTAEDGSQHHVTNPSE
FPDGKIKKIVEPMVAADIITPKEFIGAVMDLCQDHRGIMGTMEYISTDRVEMHYRIPLAEIVFDFFDQLKSRTKGYASLD
YHEDGEQSADLVKVDILIQGEKVDAFSAIVHRDKAYSYGVMMTKKLRSLIPRQQFEIPIQAAIGSRIIARENIRALRKDV
LAKCYGGDITRKRKLLEKQKAGKKRMKMLGHVEVPQEAFIAALSTGEDSNDRDTKDKIRAAQKTEG

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=599, Percent_Identity=45.7429048414023, Blast_Score=587, Evalue=1e-168,
Organism=Homo sapiens, GI94966754, Length=131, Percent_Identity=42.7480916030534, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI25306283, Length=175, Percent_Identity=42.2857142857143, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI25306287, Length=175, Percent_Identity=42.2857142857143, Blast_Score=106, Evalue=8e-23,
Organism=Homo sapiens, GI19923640, Length=175, Percent_Identity=42.2857142857143, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI18390331, Length=179, Percent_Identity=32.9608938547486, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI4503483, Length=143, Percent_Identity=38.4615384615385, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI310132016, Length=108, Percent_Identity=40.7407407407407, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI310110807, Length=108, Percent_Identity=40.7407407407407, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI310123363, Length=108, Percent_Identity=40.7407407407407, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI217272892, Length=140, Percent_Identity=37.8571428571429, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI217272894, Length=140, Percent_Identity=37.8571428571429, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI53729339, Length=215, Percent_Identity=28.8372093023256, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI53729337, Length=215, Percent_Identity=28.8372093023256, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=53.5234899328859, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=505, Percent_Identity=27.3267326732673, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1789738, Length=156, Percent_Identity=37.1794871794872, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1790835, Length=176, Percent_Identity=30.1136363636364, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1789559, Length=218, Percent_Identity=28.4403669724771, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=608, Percent_Identity=39.8026315789474, Blast_Score=469, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17533571, Length=152, Percent_Identity=42.1052631578947, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71988811, Length=158, Percent_Identity=36.0759493670886, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=158, Percent_Identity=36.0759493670886, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=153, Percent_Identity=35.2941176470588, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=168, Percent_Identity=35.7142857142857, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=136, Percent_Identity=36.0294117647059, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115532067, Length=274, Percent_Identity=25.1824817518248, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115532065, Length=274, Percent_Identity=25.1824817518248, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=597, Percent_Identity=42.5460636515913, Blast_Score=499, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6323098, Length=191, Percent_Identity=34.5549738219895, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=38.4615384615385, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=38.4615384615385, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=113, Percent_Identity=44.2477876106195, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324166, Length=146, Percent_Identity=44.5205479452055, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324761, Length=288, Percent_Identity=24.6527777777778, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI78706572, Length=597, Percent_Identity=43.7185929648241, Blast_Score=514, Evalue=1e-146,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=42.3357664233577, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24582462, Length=142, Percent_Identity=40.1408450704225, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=160, Percent_Identity=36.875, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585713, Length=160, Percent_Identity=36.875, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585709, Length=160, Percent_Identity=36.875, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221458488, Length=180, Percent_Identity=36.6666666666667, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21357743, Length=181, Percent_Identity=34.2541436464088, Blast_Score=84, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 69303; Mature: 69303

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVQHNQPGSTDQSVIRNFCIIAHIDHGKSTVADRILQLSGIVPEREMRDRFLDRMDIEQ
CEECCCCCCCHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHH
ERGITIKSQAVRVPWTFEGTEYTLGMIDTPGHVDFTYEVSRALAACEGAVLLVDATQGIE
HCCEEEECCEEEEEEEECCCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCC
AQTLSNLYMAIDHDLAIIPVLNKIDLPSAEPDKHAEEIAGLIGCEPSDVLRVSGKTGEGV
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCH
ADLLDQIVMDVPAPHGDPDAPARALIFDSVYDSYRGIVTYIRMEDGELHDREKVHMMGIG
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECC
MTHDPIEIGVISPDMTRTKALGAGEVGYIITGAKDVSQSKVGDTLTSAVRPATEPLPGYR
CCCCCEEEEEECCCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCC
DPKPMVYAGLFPIDNAQFPELRDALDKLKLNDAALIYTPETSVALGFGFRCGFLGLLHME
CCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHHHH
IVNERLSREFGLDLIQTAPNVTYDVTAEDGSQHHVTNPSEFPDGKIKKIVEPMVAADIIT
HHHHHHHHHHCCHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PKEFIGAVMDLCQDHRGIMGTMEYISTDRVEMHYRIPLAEIVFDFFDQLKSRTKGYASLD
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEECC
YHEDGEQSADLVKVDILIQGEKVDAFSAIVHRDKAYSYGVMMTKKLRSLIPRQQFEIPIQ
CCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCCCCHH
AAIGSRIIARENIRALRKDVLAKCYGGDITRKRKLLEKQKAGKKRMKMLGHVEVPQEAFI
HHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AALSTGEDSNDRDTKDKIRAAQKTEG
HHHHCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MVVQHNQPGSTDQSVIRNFCIIAHIDHGKSTVADRILQLSGIVPEREMRDRFLDRMDIEQ
CEECCCCCCCHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHH
ERGITIKSQAVRVPWTFEGTEYTLGMIDTPGHVDFTYEVSRALAACEGAVLLVDATQGIE
HCCEEEECCEEEEEEEECCCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCC
AQTLSNLYMAIDHDLAIIPVLNKIDLPSAEPDKHAEEIAGLIGCEPSDVLRVSGKTGEGV
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCH
ADLLDQIVMDVPAPHGDPDAPARALIFDSVYDSYRGIVTYIRMEDGELHDREKVHMMGIG
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECC
MTHDPIEIGVISPDMTRTKALGAGEVGYIITGAKDVSQSKVGDTLTSAVRPATEPLPGYR
CCCCCEEEEEECCCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCC
DPKPMVYAGLFPIDNAQFPELRDALDKLKLNDAALIYTPETSVALGFGFRCGFLGLLHME
CCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHHHH
IVNERLSREFGLDLIQTAPNVTYDVTAEDGSQHHVTNPSEFPDGKIKKIVEPMVAADIIT
HHHHHHHHHHCCHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PKEFIGAVMDLCQDHRGIMGTMEYISTDRVEMHYRIPLAEIVFDFFDQLKSRTKGYASLD
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEECC
YHEDGEQSADLVKVDILIQGEKVDAFSAIVHRDKAYSYGVMMTKKLRSLIPRQQFEIPIQ
CCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCCCCHH
AAIGSRIIARENIRALRKDVLAKCYGGDITRKRKLLEKQKAGKKRMKMLGHVEVPQEAFI
HHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AALSTGEDSNDRDTKDKIRAAQKTEG
HHHHCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA