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The map label for this gene is purR [H]

Identifier: 213692356

GI number: 213692356

Start: 1651635

End: 1652681

Strand: Reverse

Name: purR [H]

Synonym: Blon_1483

Alternate gene names: 213692356

Gene position: 1652681-1651635 (Counterclockwise)

Preceding gene: 213692357

Following gene: 213692355

Centisome position: 58.34

GC content: 63.61

Gene sequence:

>1047_bases
ATGGCGAAAAGCACGGGCCGCACCAGACCGGTGATCAAGGATGTGGCCAAGTTGGCCGGGGTTTCGGCACCGACCGTCTC
CCGGTATCTCAACGGCACCGTCAACGTCGCCGAGGACAAGTGCGAACGCATCGCCAAAGCCATCAGCCAGTTGGGCTATG
AGCCGAACATCGTGGCGCGTGCCTTATCGAACCGGGAGATGGATTCCGTGGTGGTGTTCGCCGCCAACCCATCCGCTTTC
TCCACATCCGAAACGAATCATGGCGTGGAGGCCGCCGCGCGAAGGCGCCGGTTCCTGCTGAACATCGTTTCCCTGGATGA
ATCCGACATGGAATCCGTCGAGGCGCGTGTGCGCATGGGCCTGTCGATCAGGCCGGCCGGAGTGATCGTCTACGAATATG
ATCGGGCCGGCATCGCCGCGCTCGAACACATTCCCAAAGACATGCCGATGGTCGTGGTGGGCGGTAGTTTCGGAGACGGC
GATTATCAGATCATCAACGCCGAAAGGGATGGCGGCCGCTGGATGACCATGCACCTGCTGGATCTCGGGCATCGCACCGT
CTTTCACGTGGGGCGCCCGGCGGGGCCGACCGCCAACACCAGGACCAACGGCTGGCGCGATGCGCTCGAGGAACGTGGCA
TCGCGGCGCCCGATCCGATCATCGTGCCCGACGATGATCTGGATGAGTCGGTCAAGGCCGGTCGCGTCCTGGGACGACGC
GACGAGGTGACCGCGATCTTCGCCGACAACGATGAGATCGCCGTGGCCGTGATCCGAGGCCTGAGGGAGGTCGGGCGCCG
CGTGCCCGATGATGTGAGCGTCGTGGGGTTCGACGACCGCAACTTCGGTGCCATGTGGAATCCCGCGTTGACCAGTTACA
CGCAGAATTTCCTGGACATGGGGGAGCGATCCTTCCGCATGCTGTTCGAGCGGATCCAAGCCAAACGCACCGGTGACGGC
ATGCCGCCCTCGCAGGTCGAAGTGGTGCAGGGCGAACCCTGTCTTCGCGCCTCCGAACGTGCGTATGACGGCGGCGAAGC
CCTGTAG

Upstream 100 bases:

>100_bases
GCCATGGCCTACAATGAGACGCGGAAAACCAAGGATTCGTGACCGGGACGCGGGTGCATGACGGCGTCCATGTCCGGCGG
CTATGGCGGGAGAGATGAGG

Downstream 100 bases:

>100_bases
CGGGCCGACCACGTCCCGCCCGTCATCGTCGCGGATCGCCTGATTCGGAATATGGACACGCGAAACGTAAATGACATGGA
GCAACGGTACTGTGTCAACA

Product: regulatory protein, LacI

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MAKSTGRTRPVIKDVAKLAGVSAPTVSRYLNGTVNVAEDKCERIAKAISQLGYEPNIVARALSNREMDSVVVFAANPSAF
STSETNHGVEAAARRRRFLLNIVSLDESDMESVEARVRMGLSIRPAGVIVYEYDRAGIAALEHIPKDMPMVVVGGSFGDG
DYQIINAERDGGRWMTMHLLDLGHRTVFHVGRPAGPTANTRTNGWRDALEERGIAAPDPIIVPDDDLDESVKAGRVLGRR
DEVTAIFADNDEIAVAVIRGLREVGRRVPDDVSVVGFDDRNFGAMWNPALTSYTQNFLDMGERSFRMLFERIQAKRTGDG
MPPSQVEVVQGEPCLRASERAYDGGEAL

Sequences:

>Translated_348_residues
MAKSTGRTRPVIKDVAKLAGVSAPTVSRYLNGTVNVAEDKCERIAKAISQLGYEPNIVARALSNREMDSVVVFAANPSAF
STSETNHGVEAAARRRRFLLNIVSLDESDMESVEARVRMGLSIRPAGVIVYEYDRAGIAALEHIPKDMPMVVVGGSFGDG
DYQIINAERDGGRWMTMHLLDLGHRTVFHVGRPAGPTANTRTNGWRDALEERGIAAPDPIIVPDDDLDESVKAGRVLGRR
DEVTAIFADNDEIAVAVIRGLREVGRRVPDDVSVVGFDDRNFGAMWNPALTSYTQNFLDMGERSFRMLFERIQAKRTGDG
MPPSQVEVVQGEPCLRASERAYDGGEAL
>Mature_347_residues
AKSTGRTRPVIKDVAKLAGVSAPTVSRYLNGTVNVAEDKCERIAKAISQLGYEPNIVARALSNREMDSVVVFAANPSAFS
TSETNHGVEAAARRRRFLLNIVSLDESDMESVEARVRMGLSIRPAGVIVYEYDRAGIAALEHIPKDMPMVVVGGSFGDGD
YQIINAERDGGRWMTMHLLDLGHRTVFHVGRPAGPTANTRTNGWRDALEERGIAAPDPIIVPDDDLDESVKAGRVLGRRD
EVTAIFADNDEIAVAVIRGLREVGRRVPDDVSVVGFDDRNFGAMWNPALTSYTQNFLDMGERSFRMLFERIQAKRTGDGM
PPSQVEVVQGEPCLRASERAYDGGEAL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=327, Percent_Identity=32.4159021406728, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1786540, Length=322, Percent_Identity=27.9503105590062, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=28.6144578313253, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=26.5625, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788474, Length=310, Percent_Identity=28.7096774193548, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=31.0344827586207, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789068, Length=290, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790369, Length=349, Percent_Identity=23.2091690544413, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48994940, Length=334, Percent_Identity=23.9520958083832, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38059; Mature: 37928

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSTGRTRPVIKDVAKLAGVSAPTVSRYLNGTVNVAEDKCERIAKAISQLGYEPNIVAR
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCHHHH
ALSNREMDSVVVFAANPSAFSTSETNHGVEAAARRRRFLLNIVSLDESDMESVEARVRMG
HHCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
LSIRPAGVIVYEYDRAGIAALEHIPKDMPMVVVGGSFGDGDYQIINAERDGGRWMTMHLL
CEECCCEEEEEEECCCHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEHHH
DLGHRTVFHVGRPAGPTANTRTNGWRDALEERGIAAPDPIIVPDDDLDESVKAGRVLGRR
HCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHCCCC
DEVTAIFADNDEIAVAVIRGLREVGRRVPDDVSVVGFDDRNFGAMWNPALTSYTQNFLDM
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
GERSFRMLFERIQAKRTGDGMPPSQVEVVQGEPCLRASERAYDGGEAL
CHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure 
AKSTGRTRPVIKDVAKLAGVSAPTVSRYLNGTVNVAEDKCERIAKAISQLGYEPNIVAR
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCHHHH
ALSNREMDSVVVFAANPSAFSTSETNHGVEAAARRRRFLLNIVSLDESDMESVEARVRMG
HHCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
LSIRPAGVIVYEYDRAGIAALEHIPKDMPMVVVGGSFGDGDYQIINAERDGGRWMTMHLL
CEECCCEEEEEEECCCHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEHHH
DLGHRTVFHVGRPAGPTANTRTNGWRDALEERGIAAPDPIIVPDDDLDESVKAGRVLGRR
HCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHCCCC
DEVTAIFADNDEIAVAVIRGLREVGRRVPDDVSVVGFDDRNFGAMWNPALTSYTQNFLDM
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
GERSFRMLFERIQAKRTGDGMPPSQVEVVQGEPCLRASERAYDGGEAL
CHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA