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The map label for this gene is nfo

Identifier: 213692253

GI number: 213692253

Start: 1541648

End: 1542502

Strand: Reverse

Name: nfo

Synonym: Blon_1373

Alternate gene names: 213692253

Gene position: 1542502-1541648 (Counterclockwise)

Preceding gene: 213692256

Following gene: 213692251

Centisome position: 54.45

GC content: 61.4

Gene sequence:

>855_bases
ATGACGGAACTGTATATCGGATCGCATCTGTCGACGGCCGGCGGATGGAATGCACTGCTGGAACGCTCCCATGAGGAGGG
CGGAACCGCGTTCGCGTTCTTCCCGCGCTCCCCGTACGGCAAGCGTTCGAAGGCGCTCGACCCGGCCGACGCGGCAACGT
TCGGCGCACGTCTGAAGGCTGAGGACTATGGGCCGTTGGTCGTCCACGCGCCGTACGTCTACAATCTGGCAGGCAAGGAT
GAGGCCAAGCGCGCATTCGCGATCGAGGCGTTGGCCGAGGACATCGAGCTGCTGACCGCCATCCGCGAGGCCGGCCAGGA
GGTGTATATCAATATTCATCCCGGCGCACATGTGGGCCAAGGCACCAAGACCGGCTGCCAGCTGATCAGCGAGGGACTGA
ATCAGGTGTTCGAGCGTACGACCGGCGTCATGGTGCTTCTGGAGACCATGGCCGGCAAAGGCACGGAATGCGGGCGCAAC
TTTGAGGAGCTCGCCACGATCATGAACGGCGTGAAGAACAAGGCGAACGTCGGCGTCACGTTCGACACCTGCCATGTGCT
GGATGCCGGCTACGATTTGGTGAACGACTACGACGGTGTGATGCGCCAGTTGGATGAGGCGATCGGCTTGGCCAAGGTCA
AGGCGATTCACGTGAACGATTCGCAGTTTGGCCTCGACTCGCATAAGGATCGTCACGCCAACATCGGGCAGGGCCAGCTC
GGCATCCCGTTTTTCACGCGACTGGTCAACGACCCGATCATGGCCAAGCTCCCGATGATCCTCGAAACCAAGGAGCAGAC
GCCCGCCACGCATCGCGACGAGATCGAGTTGCTGCGCGGATTGGTGCAAAAGTAG

Upstream 100 bases:

>100_bases
GAACAGTTGTGTCTCGTGTCGGTTGTGCGGGCGGCGCGCGATACCGCCTACCATATGATAGCGGTAACACGAACAGCGAG
CGGAACATGAGGAGTTTGGA

Downstream 100 bases:

>100_bases
TCGTAGTCGACCAATGTCGAGCAGAAGAGACCTGTTTTTGACTGAGGGCCATACGAGGTTAGCTATCAGCGGGGCATTGA
TTGATCCGTCTTCATATACA

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTELYIGSHLSTAGGWNALLERSHEEGGTAFAFFPRSPYGKRSKALDPADAATFGARLKAEDYGPLVVHAPYVYNLAGKD
EAKRAFAIEALAEDIELLTAIREAGQEVYINIHPGAHVGQGTKTGCQLISEGLNQVFERTTGVMVLLETMAGKGTECGRN
FEELATIMNGVKNKANVGVTFDTCHVLDAGYDLVNDYDGVMRQLDEAIGLAKVKAIHVNDSQFGLDSHKDRHANIGQGQL
GIPFFTRLVNDPIMAKLPMILETKEQTPATHRDEIELLRGLVQK

Sequences:

>Translated_284_residues
MTELYIGSHLSTAGGWNALLERSHEEGGTAFAFFPRSPYGKRSKALDPADAATFGARLKAEDYGPLVVHAPYVYNLAGKD
EAKRAFAIEALAEDIELLTAIREAGQEVYINIHPGAHVGQGTKTGCQLISEGLNQVFERTTGVMVLLETMAGKGTECGRN
FEELATIMNGVKNKANVGVTFDTCHVLDAGYDLVNDYDGVMRQLDEAIGLAKVKAIHVNDSQFGLDSHKDRHANIGQGQL
GIPFFTRLVNDPIMAKLPMILETKEQTPATHRDEIELLRGLVQK
>Mature_283_residues
TELYIGSHLSTAGGWNALLERSHEEGGTAFAFFPRSPYGKRSKALDPADAATFGARLKAEDYGPLVVHAPYVYNLAGKDE
AKRAFAIEALAEDIELLTAIREAGQEVYINIHPGAHVGQGTKTGCQLISEGLNQVFERTTGVMVLLETMAGKGTECGRNF
EELATIMNGVKNKANVGVTFDTCHVLDAGYDLVNDYDGVMRQLDEAIGLAKVKAIHVNDSQFGLDSHKDRHANIGQGQLG
IPFFTRLVNDPIMAKLPMILETKEQTPATHRDEIELLRGLVQK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=286, Percent_Identity=30.7692307692308, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17531193, Length=266, Percent_Identity=37.593984962406, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6322735, Length=289, Percent_Identity=28.3737024221453, Blast_Score=140, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_BIFLI (B7GRN1)

Other databases:

- EMBL:   CP001095
- RefSeq:   YP_002322839.1
- ProteinModelPortal:   B7GRN1
- GeneID:   7054027
- GenomeReviews:   CP001095_GR
- KEGG:   bln:Blon_1373
- HOGENOM:   HBG565018
- ProtClustDB:   CLSK2304230
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 30939; Mature: 30807

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELYIGSHLSTAGGWNALLERSHEEGGTAFAFFPRSPYGKRSKALDPADAATFGARLKA
CCEEEECCCCCCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHCCEEEC
EDYGPLVVHAPYVYNLAGKDEAKRAFAIEALAEDIELLTAIREAGQEVYINIHPGAHVGQ
CCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
GTKTGCQLISEGLNQVFERTTGVMVLLETMAGKGTECGRNFEELATIMNGVKNKANVGVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEE
FDTCHVLDAGYDLVNDYDGVMRQLDEAIGLAKVKAIHVNDSQFGLDSHKDRHANIGQGQL
EHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCCCCCCCCCCCCC
GIPFFTRLVNDPIMAKLPMILETKEQTPATHRDEIELLRGLVQK
CHHHHHHHHCCCHHHHCCHHEECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TELYIGSHLSTAGGWNALLERSHEEGGTAFAFFPRSPYGKRSKALDPADAATFGARLKA
CEEEECCCCCCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHCCEEEC
EDYGPLVVHAPYVYNLAGKDEAKRAFAIEALAEDIELLTAIREAGQEVYINIHPGAHVGQ
CCCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
GTKTGCQLISEGLNQVFERTTGVMVLLETMAGKGTECGRNFEELATIMNGVKNKANVGVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEE
FDTCHVLDAGYDLVNDYDGVMRQLDEAIGLAKVKAIHVNDSQFGLDSHKDRHANIGQGQL
EHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCCCCCCCCCCCCC
GIPFFTRLVNDPIMAKLPMILETKEQTPATHRDEIELLRGLVQK
CHHHHHHHHCCCHHHHCCHHEECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA