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The map label for this gene is trpA [H]

Identifier: 213692250

GI number: 213692250

Start: 1537645

End: 1538514

Strand: Reverse

Name: trpA [H]

Synonym: Blon_1370

Alternate gene names: 213692250

Gene position: 1538514-1537645 (Counterclockwise)

Preceding gene: 213692251

Following gene: 213692249

Centisome position: 54.31

GC content: 66.44

Gene sequence:

>870_bases
ATGACCAACGAAGCGAATACGCCGTCCGGCCAGCCGCTGGGCATCAGCCACAAGCCCAGCAAGTCGGGCGCTATGTTCAC
CAAGTTCAAGGCCGAGAACAAGCCCGCCTTCATCGGCTACCTGCCCTATGGCTTCCCCAACCCAGACGTCTCGCTTGACG
CCTTCAAAACGATGGTCGAGCACGGCGTCGATGCCGTGGAGATCGGCCTGCCGTACTCCGACCCGGTCATGGACGGCCCG
GTCATCCAGGCTGCCGCCTCCATCGCCCTGAACAACGGCGAGACCATCAAGCGCGTGTTCGAGGCCGTCGAAACCGTGGC
CAACGCCGGCGGCGTGCCGCTCATCATGAGCTACTGGAACCTCGTATACCACTACGGCGTCGAGCGTTTCGCCCGTGACT
TCGAGAACGCCGGCGGCGCCGGCCTCATCACCCCGGACCTTATCCCGGACGAGGCGGGGGAGTGGATTGAAGCCTCCGAC
CGTCACGGACTCGACCGCATCTTCCTGGTCTCCCCGGACTCGTCCACCGAGCGCCTCGAAACCGTGGCCCGCAACGCGCG
TGGCTTCGTGTACGCGGCCGCCCGCATGGGCGTCACCGGGGAACGCGCCACCATCGACGCCTCACCCGAAGAGCTTGTGG
TGCGCACCCGTCGCGCGGGCGCTGAGAACGTGTGCGTGGGCATCGGCGTATCCACCGCGGAACAGGGCGCCCGGGTCGGC
GCCTACGCAGATGGCGTCATCGTCGGTTCCGCGCTCGTGCACACCATGCTCGCGGACGACGACAAGACCGCACGCGACCC
GAAGGAAGGCTTGAAGCTGCTCGCCGCCAAGAGCGAGGAACTGGCCGAAGGCATCCACAACGCCCGGTAA

Upstream 100 bases:

>100_bases
AGTGGTTTGGCTACCTGACCGACGATCAGGCCGCCGCGCTTGATGTGACCGGTGTCAACGGCAACACCGTTGCCTGAACA
AGAAGAGGAGATTGTTGATT

Downstream 100 bases:

>100_bases
ACACCCGGTGCGCCTCACGCGGATTGTGCCGTGACACGGTAGGGTAGTCACAGAGCATCAAGCACAACCCGCGCGCGGAC
ACACCATGAGCACCAGCAAG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTNEANTPSGQPLGISHKPSKSGAMFTKFKAENKPAFIGYLPYGFPNPDVSLDAFKTMVEHGVDAVEIGLPYSDPVMDGP
VIQAAASIALNNGETIKRVFEAVETVANAGGVPLIMSYWNLVYHYGVERFARDFENAGGAGLITPDLIPDEAGEWIEASD
RHGLDRIFLVSPDSSTERLETVARNARGFVYAAARMGVTGERATIDASPEELVVRTRRAGAENVCVGIGVSTAEQGARVG
AYADGVIVGSALVHTMLADDDKTARDPKEGLKLLAAKSEELAEGIHNAR

Sequences:

>Translated_289_residues
MTNEANTPSGQPLGISHKPSKSGAMFTKFKAENKPAFIGYLPYGFPNPDVSLDAFKTMVEHGVDAVEIGLPYSDPVMDGP
VIQAAASIALNNGETIKRVFEAVETVANAGGVPLIMSYWNLVYHYGVERFARDFENAGGAGLITPDLIPDEAGEWIEASD
RHGLDRIFLVSPDSSTERLETVARNARGFVYAAARMGVTGERATIDASPEELVVRTRRAGAENVCVGIGVSTAEQGARVG
AYADGVIVGSALVHTMLADDDKTARDPKEGLKLLAAKSEELAEGIHNAR
>Mature_288_residues
TNEANTPSGQPLGISHKPSKSGAMFTKFKAENKPAFIGYLPYGFPNPDVSLDAFKTMVEHGVDAVEIGLPYSDPVMDGPV
IQAAASIALNNGETIKRVFEAVETVANAGGVPLIMSYWNLVYHYGVERFARDFENAGGAGLITPDLIPDEAGEWIEASDR
HGLDRIFLVSPDSSTERLETVARNARGFVYAAARMGVTGERATIDASPEELVVRTRRAGAENVCVGIGVSTAEQGARVGA
YADGVIVGSALVHTMLADDDKTARDPKEGLKLLAAKSEELAEGIHNAR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=214, Percent_Identity=29.9065420560748, Blast_Score=114, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6321412, Length=237, Percent_Identity=39.2405063291139, Blast_Score=168, Evalue=1e-42,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 30746; Mature: 30615

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNEANTPSGQPLGISHKPSKSGAMFTKFKAENKPAFIGYLPYGFPNPDVSLDAFKTMVE
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHH
HGVDAVEIGLPYSDPVMDGPVIQAAASIALNNGETIKRVFEAVETVANAGGVPLIMSYWN
CCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
LVYHYGVERFARDFENAGGAGLITPDLIPDEAGEWIEASDRHGLDRIFLVSPDSSTERLE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHH
TVARNARGFVYAAARMGVTGERATIDASPEELVVRTRRAGAENVCVGIGVSTAEQGARVG
HHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCC
AYADGVIVGSALVHTMLADDDKTARDPKEGLKLLAAKSEELAEGIHNAR
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
TNEANTPSGQPLGISHKPSKSGAMFTKFKAENKPAFIGYLPYGFPNPDVSLDAFKTMVE
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHH
HGVDAVEIGLPYSDPVMDGPVIQAAASIALNNGETIKRVFEAVETVANAGGVPLIMSYWN
CCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
LVYHYGVERFARDFENAGGAGLITPDLIPDEAGEWIEASDRHGLDRIFLVSPDSSTERLE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHH
TVARNARGFVYAAARMGVTGERATIDASPEELVVRTRRAGAENVCVGIGVSTAEQGARVG
HHHHCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCC
AYADGVIVGSALVHTMLADDDKTARDPKEGLKLLAAKSEELAEGIHNAR
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]