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The map label for this gene is murE [H]

Identifier: 213691785

GI number: 213691785

Start: 1058252

End: 1059805

Strand: Reverse

Name: murE [H]

Synonym: Blon_0893

Alternate gene names: 213691785

Gene position: 1059805-1058252 (Counterclockwise)

Preceding gene: 213691788

Following gene: 213691784

Centisome position: 37.41

GC content: 61.33

Gene sequence:

>1554_bases
ATGGCTTTGACTTTGGCTTCGGCCGCTGACCTGCTCAAGGAACACCATCTGCTGCGTGAAATCATCCAAGGGGATGTCTG
GACCGACGACCCGGCCCGTATCGCTTCGACGGATGAACCGTTCGCCGGCATCACCTACGACACCCGCAAGGTCACGCCGG
GCACCCTGCTGTGCTGCAAGGGCCGATTCAAGGCCGAGTATCTGAACGGCATCGACGAAGCCGGCCTGGCCGCATACGTG
GCCGAAACCGAGTACTCAGCCGCAACCGCCGCCCCCGGCCTGATCGTCAACGACGCACGCAAGGCGATGAGCCTGCTGTC
CGCCGCGTTCTACGGATATCCGCAGAACGAGCTCACGGTCATCGGCGTCACCGGCACCAAGGGCAAGACGACCACCTCGT
ACTTCACGCAGGCGCTTATCAACGCCGTCTCCGGTGGCAAGGCCGCATTGTTCTCCTCGGTCGATAATTGCCTTGACGGC
CACACCTACGTCGAGTCCGATCTGACCACGCCCGAATCGATGGACGCCTTCCGCATGATGCGCGAGGCGGCCGATAACGG
CATGGAGTATCTGGTGATGGAGGTCTCCTCCCAGGCGTACAAGGTGGACCGCGTCTATGGCCTGACTTTCGATGTGGCCG
CGTTCCTCAACATCTCCCCCGACCACATCAGTCCGATCGAACACCCCACGTTCGAGGATTACCTGTATTGCAAGCGCCAG
ATCATCGCCAACGCGAAGTCGCTGGTACTCGGCGCGGACAGTCTGCACGCCGATCTGTTGCGGGAGGACGCGGCGGCAGC
CGGCATCGGCGCAACCACCTTCGCCCTGCATGACGCGGATAATGCCGGCACCAGTGCGGACGTCGTCGCCTGGCCCGCCG
ACACGGCGCACGCCTCCTTCCACATCGCCGACGGAGATCAGACCCTTGGCGACTACCATCTGTCCATCGATGGCGACTTC
AACTACCTCAATGCGGCGGCGGCCATCGCCATCGCCCATGCGGCTGGCGTATCGCTCGATGACGCCGGCGCACTGCACGC
CATCGAATCCGTACGTATCGCCGGCCGTATGGAACAGTTCCGCGATCCGCAGTCGAACACGCTCGCCATCGTCGATTATG
CGCACAACTACGCATCCGTCACCGCGCTGCTTGACTTCGTCTACGAGCGTTGGGGCGAGGAAAACCCGCGTATCACGCTG
GTGACCGGATCGGCCGGCAACAAGGCCTACGATCGTCGTAAGGAAATCGTCGAGGCTGCCGAGAACCGTATCGCGAACTT
CATTTTCACCGCCGAAGATACGGACACCGAGCCGTTCATCGACATCTGCATGGAAATGCAAGGCTACATCACGAACAAGG
ATGTGGCCTCCACGGTGATTTCCGACCGTCCGACGGCCATCACCAACGCCATTTACGATGCCCGTGCGCACGCCGACCGA
TTCAACATCCTGCTGATTATCGGCAAGGGCAACGAGCGTTGGATCAAGGACCATCACAAGCATGTGCCGTTCGACGGCGA
CGATCACGTCGTCGAACGCATGTTTGGCTTGTGA

Upstream 100 bases:

>100_bases
AAATCATGTGCCCATATCGTGGTCGATGACGTGTGAGCCACAACACGCTGCACCCCACCTGTAGAATGGCGTATCGGCTA
GATAATAGGGAGAAACAATT

Downstream 100 bases:

>100_bases
CGGGATTCCGGCCAATCAGATCCTGATCGGATCCCGATTGGCCCACATCAGATCACGCGCAATACGACCAAACCAGAAAC
ACGACATACGGATACGAACG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MALTLASAADLLKEHHLLREIIQGDVWTDDPARIASTDEPFAGITYDTRKVTPGTLLCCKGRFKAEYLNGIDEAGLAAYV
AETEYSAATAAPGLIVNDARKAMSLLSAAFYGYPQNELTVIGVTGTKGKTTTSYFTQALINAVSGGKAALFSSVDNCLDG
HTYVESDLTTPESMDAFRMMREAADNGMEYLVMEVSSQAYKVDRVYGLTFDVAAFLNISPDHISPIEHPTFEDYLYCKRQ
IIANAKSLVLGADSLHADLLREDAAAAGIGATTFALHDADNAGTSADVVAWPADTAHASFHIADGDQTLGDYHLSIDGDF
NYLNAAAAIAIAHAAGVSLDDAGALHAIESVRIAGRMEQFRDPQSNTLAIVDYAHNYASVTALLDFVYERWGEENPRITL
VTGSAGNKAYDRRKEIVEAAENRIANFIFTAEDTDTEPFIDICMEMQGYITNKDVASTVISDRPTAITNAIYDARAHADR
FNILLIIGKGNERWIKDHHKHVPFDGDDHVVERMFGL

Sequences:

>Translated_517_residues
MALTLASAADLLKEHHLLREIIQGDVWTDDPARIASTDEPFAGITYDTRKVTPGTLLCCKGRFKAEYLNGIDEAGLAAYV
AETEYSAATAAPGLIVNDARKAMSLLSAAFYGYPQNELTVIGVTGTKGKTTTSYFTQALINAVSGGKAALFSSVDNCLDG
HTYVESDLTTPESMDAFRMMREAADNGMEYLVMEVSSQAYKVDRVYGLTFDVAAFLNISPDHISPIEHPTFEDYLYCKRQ
IIANAKSLVLGADSLHADLLREDAAAAGIGATTFALHDADNAGTSADVVAWPADTAHASFHIADGDQTLGDYHLSIDGDF
NYLNAAAAIAIAHAAGVSLDDAGALHAIESVRIAGRMEQFRDPQSNTLAIVDYAHNYASVTALLDFVYERWGEENPRITL
VTGSAGNKAYDRRKEIVEAAENRIANFIFTAEDTDTEPFIDICMEMQGYITNKDVASTVISDRPTAITNAIYDARAHADR
FNILLIIGKGNERWIKDHHKHVPFDGDDHVVERMFGL
>Mature_516_residues
ALTLASAADLLKEHHLLREIIQGDVWTDDPARIASTDEPFAGITYDTRKVTPGTLLCCKGRFKAEYLNGIDEAGLAAYVA
ETEYSAATAAPGLIVNDARKAMSLLSAAFYGYPQNELTVIGVTGTKGKTTTSYFTQALINAVSGGKAALFSSVDNCLDGH
TYVESDLTTPESMDAFRMMREAADNGMEYLVMEVSSQAYKVDRVYGLTFDVAAFLNISPDHISPIEHPTFEDYLYCKRQI
IANAKSLVLGADSLHADLLREDAAAAGIGATTFALHDADNAGTSADVVAWPADTAHASFHIADGDQTLGDYHLSIDGDFN
YLNAAAAIAIAHAAGVSLDDAGALHAIESVRIAGRMEQFRDPQSNTLAIVDYAHNYASVTALLDFVYERWGEENPRITLV
TGSAGNKAYDRRKEIVEAAENRIANFIFTAEDTDTEPFIDICMEMQGYITNKDVASTVISDRPTAITNAIYDARAHADRF
NILLIIGKGNERWIKDHHKHVPFDGDDHVVERMFGL

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=462, Percent_Identity=25.3246753246753, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.13

Molecular weight: Translated: 56172; Mature: 56041

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTLASAADLLKEHHLLREIIQGDVWTDDPARIASTDEPFAGITYDTRKVTPGTLLCCK
CEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEC
GRFKAEYLNGIDEAGLAAYVAETEYSAATAAPGLIVNDARKAMSLLSAAFYGYPQNELTV
CCCHHHHHCCCCCCCCEEEEECCCCCHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEE
IGVTGTKGKTTTSYFTQALINAVSGGKAALFSSVDNCLDGHTYVESDLTTPESMDAFRMM
EEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHH
REAADNGMEYLVMEVSSQAYKVDRVYGLTFDVAAFLNISPDHISPIEHPTFEDYLYCKRQ
HHHHCCCCEEEEEECCCCCEEEHEEECCEEEEHHHEECCCCCCCCCCCCCHHHHHHHHHH
IIANAKSLVLGADSLHADLLREDAAAAGIGATTFALHDADNAGTSADVVAWPADTAHASF
HHHCCHHEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCEE
HIADGDQTLGDYHLSIDGDFNYLNAAAAIAIAHAAGVSLDDAGALHAIESVRIAGRMEQF
EEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
RDPQSNTLAIVDYAHNYASVTALLDFVYERWGEENPRITLVTGSAGNKAYDRRKEIVEAA
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
ENRIANFIFTAEDTDTEPFIDICMEMQGYITNKDVASTVISDRPTAITNAIYDARAHADR
HHHHEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCC
FNILLIIGKGNERWIKDHHKHVPFDGDDHVVERMFGL
EEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
ALTLASAADLLKEHHLLREIIQGDVWTDDPARIASTDEPFAGITYDTRKVTPGTLLCCK
EEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEC
GRFKAEYLNGIDEAGLAAYVAETEYSAATAAPGLIVNDARKAMSLLSAAFYGYPQNELTV
CCCHHHHHCCCCCCCCEEEEECCCCCHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEE
IGVTGTKGKTTTSYFTQALINAVSGGKAALFSSVDNCLDGHTYVESDLTTPESMDAFRMM
EEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHH
REAADNGMEYLVMEVSSQAYKVDRVYGLTFDVAAFLNISPDHISPIEHPTFEDYLYCKRQ
HHHHCCCCEEEEEECCCCCEEEHEEECCEEEEHHHEECCCCCCCCCCCCCHHHHHHHHHH
IIANAKSLVLGADSLHADLLREDAAAAGIGATTFALHDADNAGTSADVVAWPADTAHASF
HHHCCHHEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCEE
HIADGDQTLGDYHLSIDGDFNYLNAAAAIAIAHAAGVSLDDAGALHAIESVRIAGRMEQF
EEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
RDPQSNTLAIVDYAHNYASVTALLDFVYERWGEENPRITLVTGSAGNKAYDRRKEIVEAA
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
ENRIANFIFTAEDTDTEPFIDICMEMQGYITNKDVASTVISDRPTAITNAIYDARAHADR
HHHHEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCC
FNILLIIGKGNERWIKDHHKHVPFDGDDHVVERMFGL
EEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 0.097 {dihydrouridine-5´-dip

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]