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The map label for this gene is folC [H]

Identifier: 213691782

GI number: 213691782

Start: 1051660

End: 1053285

Strand: Direct

Name: folC [H]

Synonym: Blon_0890

Alternate gene names: 213691782

Gene position: 1051660-1053285 (Clockwise)

Preceding gene: 213691778

Following gene: 213691783

Centisome position: 37.13

GC content: 63.28

Gene sequence:

>1626_bases
ATGTCATTCGAGCATCCGAATCGGAACAATGAGAGCATGCGGGACGTCGAACTGGACATCATGAGCCGCCCGCCGGAGCA
TAACACCACCAATCTGGATCTCGACCGGATGAACCTGATGCTCGACATCCTGGGCCACCCGGAGCAGTCGTTCCGCGTGA
TTCATATCACCGGCACCAACGGCAAAGGCTCCACCGCACGCATGGCCGAGGCCATCTGCCGCGCCTACGGTATGCGCACC
GGCCTGTACACTTCGCCGCATCTGGAGAAGATCAACGAGCGTATCGCCATCAACGGCCAGCAGCTGTCCGACGACGATTT
CATCGACATCTGGGACCAGACCAAGGATCTCGTGGCCCTGGTGGACGCCAAGATGGAGGAGCAAGGCAAGCCGAAGATGA
GCTTCTTCGAGGTGCTCACCGCCATGGCCATCTGGAAGTTCGCCGACACGCCCGTGGACGTGGCCATCGTCGAGGTCGGC
ATGGGAGGCCTGTGGGACGCCACCAACGTGCTCAACGCGGATGCGGCCATCATCGGTCCGGTGGACATGGACCACATGCA
GTGGCTGGGCGACACCGTCGAACAGATCGCCACCGAGAAGGCCGGCATCATCAAGCCGAACTGCACCGCCATCATCGGCC
CGCAGCCGCATGAGGAAGCCGTCATGCCGATTCTCACCGAAGCCGCCGAACGCAACCACGCCACGCTCGTGCGCGACGGT
TACGAAATGACCGTGTCCGACCGCATGGCTGCAGTCGGCGGACAAGTCGCCACCCTGACCACGCCCAACGGCACTTACGA
GGGCGTGCCGATCGCCAAGTTCGGCGAGCATCAGGCCCATAACGCACTTGCCGCGCTCGTCGCCGCCGAAGTGGTGATTC
CGGTCAATGGCCCGCTGGACGGCGACTTGGTGGCCGAGGCGTTGAGCTCGGTCAAGATTCCCGGCCGCATCGAACAGATC
CGCACCTCGCCGACCATCATTCTGGATGGCGGACACAACGTGAATGCCGCCGAAGCCCTACGCAAGGCCATCGAGGAAAG
CTACGACTTCAAGCAGCTGGTCGGTGTGGTCGCCATGATGCGCGACAAGCAGGTCGAGGAATATCTGGGTGTTCTGGAGC
CGATCCTGAGCTCCGTGGTGGTCACCGAGAACTCGTGGCGCGAGCGTGTGATGCCGGCCGATGAACTTGAGAAGATTGCC
GTGGACGTGTTCGGCCGTGACCGTGTGATCAAGGAAGCGAACCTGCCCGACGCGATTCAGACTGCCGTCAACATGGTCGA
TGCCGAAGACGAGCTGGGCGTGGGCTACGGCCACGGCGTGCTTATCTGCGGCAGCTTCGTCACCGCCGGCGACGCACGCC
TGATGCTTGAGGAACACGCCAGCCCCACGATGAGGCAGGCCATGGCCGTCCATCAGCCGGCGGTCGACCCGGACGATTCG
GATCGGCCGGCAGACAAGGCCGAGGATGAGGCCGCCGACAACCTCGAGGATTCGGTAAGCCCGGACGACTTCGACGTATT
CGACGTCCTCGGCCTCGGTAAGGAACAGGCCAGTGACGCCGGTAACGCCGGCACCGGCACGGCCAGTGCCGACACAGGCA
CCGACGATTCCGCCGACGCCCGGTAA

Upstream 100 bases:

>100_bases
TGCGGGCATGCGTAGACATGACGAGAATGGCGCGAACACGTAAGTGGCATACGCACTCCGAACGGTGCAACAGCAACACG
AGCAGAGGTTAGAAGAGAAT

Downstream 100 bases:

>100_bases
CCCGAATCCAAGGCATATCGGATACCCGGACACACGGAAAACAATCTCACGCCATGTATCTCAAGGAACTCACCCTCCGC
GGATTCAAATCCTTCGCCTC

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSFEHPNRNNESMRDVELDIMSRPPEHNTTNLDLDRMNLMLDILGHPEQSFRVIHITGTNGKGSTARMAEAICRAYGMRT
GLYTSPHLEKINERIAINGQQLSDDDFIDIWDQTKDLVALVDAKMEEQGKPKMSFFEVLTAMAIWKFADTPVDVAIVEVG
MGGLWDATNVLNADAAIIGPVDMDHMQWLGDTVEQIATEKAGIIKPNCTAIIGPQPHEEAVMPILTEAAERNHATLVRDG
YEMTVSDRMAAVGGQVATLTTPNGTYEGVPIAKFGEHQAHNALAALVAAEVVIPVNGPLDGDLVAEALSSVKIPGRIEQI
RTSPTIILDGGHNVNAAEALRKAIEESYDFKQLVGVVAMMRDKQVEEYLGVLEPILSSVVVTENSWRERVMPADELEKIA
VDVFGRDRVIKEANLPDAIQTAVNMVDAEDELGVGYGHGVLICGSFVTAGDARLMLEEHASPTMRQAMAVHQPAVDPDDS
DRPADKAEDEAADNLEDSVSPDDFDVFDVLGLGKEQASDAGNAGTGTASADTGTDDSADAR

Sequences:

>Translated_541_residues
MSFEHPNRNNESMRDVELDIMSRPPEHNTTNLDLDRMNLMLDILGHPEQSFRVIHITGTNGKGSTARMAEAICRAYGMRT
GLYTSPHLEKINERIAINGQQLSDDDFIDIWDQTKDLVALVDAKMEEQGKPKMSFFEVLTAMAIWKFADTPVDVAIVEVG
MGGLWDATNVLNADAAIIGPVDMDHMQWLGDTVEQIATEKAGIIKPNCTAIIGPQPHEEAVMPILTEAAERNHATLVRDG
YEMTVSDRMAAVGGQVATLTTPNGTYEGVPIAKFGEHQAHNALAALVAAEVVIPVNGPLDGDLVAEALSSVKIPGRIEQI
RTSPTIILDGGHNVNAAEALRKAIEESYDFKQLVGVVAMMRDKQVEEYLGVLEPILSSVVVTENSWRERVMPADELEKIA
VDVFGRDRVIKEANLPDAIQTAVNMVDAEDELGVGYGHGVLICGSFVTAGDARLMLEEHASPTMRQAMAVHQPAVDPDDS
DRPADKAEDEAADNLEDSVSPDDFDVFDVLGLGKEQASDAGNAGTGTASADTGTDDSADAR
>Mature_540_residues
SFEHPNRNNESMRDVELDIMSRPPEHNTTNLDLDRMNLMLDILGHPEQSFRVIHITGTNGKGSTARMAEAICRAYGMRTG
LYTSPHLEKINERIAINGQQLSDDDFIDIWDQTKDLVALVDAKMEEQGKPKMSFFEVLTAMAIWKFADTPVDVAIVEVGM
GGLWDATNVLNADAAIIGPVDMDHMQWLGDTVEQIATEKAGIIKPNCTAIIGPQPHEEAVMPILTEAAERNHATLVRDGY
EMTVSDRMAAVGGQVATLTTPNGTYEGVPIAKFGEHQAHNALAALVAAEVVIPVNGPLDGDLVAEALSSVKIPGRIEQIR
TSPTIILDGGHNVNAAEALRKAIEESYDFKQLVGVVAMMRDKQVEEYLGVLEPILSSVVVTENSWRERVMPADELEKIAV
DVFGRDRVIKEANLPDAIQTAVNMVDAEDELGVGYGHGVLICGSFVTAGDARLMLEEHASPTMRQAMAVHQPAVDPDDSD
RPADKAEDEAADNLEDSVSPDDFDVFDVLGLGKEQASDAGNAGTGTASADTGTDDSADAR

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=345, Percent_Identity=31.5942028985507, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI66932984, Length=345, Percent_Identity=31.5942028985507, Blast_Score=139, Evalue=1e-32,
Organism=Escherichia coli, GI1788654, Length=350, Percent_Identity=32.8571428571429, Blast_Score=163, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17553148, Length=333, Percent_Identity=30.03003003003, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71984923, Length=333, Percent_Identity=30.03003003003, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17553150, Length=333, Percent_Identity=30.03003003003, Blast_Score=128, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323760, Length=450, Percent_Identity=27.5555555555556, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324815, Length=250, Percent_Identity=32.8, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24641571, Length=200, Percent_Identity=35.5, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581568, Length=156, Percent_Identity=37.1794871794872, Blast_Score=99, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 58458; Mature: 58327

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFEHPNRNNESMRDVELDIMSRPPEHNTTNLDLDRMNLMLDILGHPEQSFRVIHITGTN
CCCCCCCCCCCCHHHEEEHECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCC
GKGSTARMAEAICRAYGMRTGLYTSPHLEKINERIAINGQQLSDDDFIDIWDQTKDLVAL
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCEEHHHHHHHHHHH
VDAKMEEQGKPKMSFFEVLTAMAIWKFADTPVDVAIVEVGMGGLWDATNVLNADAAIIGP
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCCEEECC
VDMDHMQWLGDTVEQIATEKAGIIKPNCTAIIGPQPHEEAVMPILTEAAERNHATLVRDG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECC
YEMTVSDRMAAVGGQVATLTTPNGTYEGVPIAKFGEHQAHNALAALVAAEVVIPVNGPLD
CEEEHHHHHHHHCCEEEEEECCCCCCCCCCCHHCCCHHHHHHHHHHHHHHEEEECCCCCC
GDLVAEALSSVKIPGRIEQIRTSPTIILDGGHNVNAAEALRKAIEESYDFKQLVGVVAMM
HHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
RDKQVEEYLGVLEPILSSVVVTENSWRERVMPADELEKIAVDVFGRDRVIKEANLPDAIQ
HHHHHHHHHHHHHHHHHHEEECCCCHHHHCCCHHHHHHHHHHHCCCCCCHHCCCCCHHHH
TAVNMVDAEDELGVGYGHGVLICGSFVTAGDARLMLEEHASPTMRQAMAVHQPAVDPDDS
HHHHHHCCCCCCCCCCCCCEEEECCHHHCCCCEEHHHHCCCHHHHHHHHHHCCCCCCCCC
DRPADKAEDEAADNLEDSVSPDDFDVFDVLGLGKEQASDAGNAGTGTASADTGTDDSADA
CCCCCCCHHHHHHCHHHCCCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
R
C
>Mature Secondary Structure 
SFEHPNRNNESMRDVELDIMSRPPEHNTTNLDLDRMNLMLDILGHPEQSFRVIHITGTN
CCCCCCCCCCCHHHEEEHECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCC
GKGSTARMAEAICRAYGMRTGLYTSPHLEKINERIAINGQQLSDDDFIDIWDQTKDLVAL
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCEEHHHHHHHHHHH
VDAKMEEQGKPKMSFFEVLTAMAIWKFADTPVDVAIVEVGMGGLWDATNVLNADAAIIGP
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCCEEECC
VDMDHMQWLGDTVEQIATEKAGIIKPNCTAIIGPQPHEEAVMPILTEAAERNHATLVRDG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECC
YEMTVSDRMAAVGGQVATLTTPNGTYEGVPIAKFGEHQAHNALAALVAAEVVIPVNGPLD
CEEEHHHHHHHHCCEEEEEECCCCCCCCCCCHHCCCHHHHHHHHHHHHHHEEEECCCCCC
GDLVAEALSSVKIPGRIEQIRTSPTIILDGGHNVNAAEALRKAIEESYDFKQLVGVVAMM
HHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
RDKQVEEYLGVLEPILSSVVVTENSWRERVMPADELEKIAVDVFGRDRVIKEANLPDAIQ
HHHHHHHHHHHHHHHHHHEEECCCCHHHHCCCHHHHHHHHHHHCCCCCCHHCCCCCHHHH
TAVNMVDAEDELGVGYGHGVLICGSFVTAGDARLMLEEHASPTMRQAMAVHQPAVDPDDS
HHHHHHCCCCCCCCCCCCCEEEECCHHHCCCCEEHHHHCCCHHHHHHHHHHCCCCCCCCC
DRPADKAEDEAADNLEDSVSPDDFDVFDVLGLGKEQASDAGNAGTGTASADTGTDDSADA
CCCCCCCHHHHHHCHHHCCCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]