Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 213691748

GI number: 213691748

Start: 1006794

End: 1008239

Strand: Direct

Name: murD [H]

Synonym: Blon_0854

Alternate gene names: 213691748

Gene position: 1006794-1008239 (Clockwise)

Preceding gene: 213691747

Following gene: 213691749

Centisome position: 35.54

GC content: 62.24

Gene sequence:

>1446_bases
ATGCAAGTAGCGGATAAGACAGTGGTGATCGCCGGACTGGGGGTATCCGGCACCTCGTTGGCGGAGGTATTGAGGGAACG
CGGCGCCCACGTGATTGGCGTCGACGAGAGGAAGCCCGAGGCTGATCTCCGCTCCTTTGATGACGTGGACTGGGACCATG
TGGACTACGTCATGTCGTCCCCGGTGTTCAACCCCCGCACCCCGTTCGTGCTGGAAGCCCAACGCCGTGGCATCCCCGTG
ATGAGCGAGGTGGAATTCGCATGGCAGCTGCGTGTGAATAACGAGCGCACCGGTACTCCGGCTCCATGGATCGGCATCAC
CGGCACCAACGGCAAAACCTCCACCACCGAGATGACCTCGGAAATGCTCACCGCCTGCGGACTCGACGCGCCGACCGCCG
GCAATATCGCCTCCGGAGACATGAGCATGAGCCTGTCTCGTTGCGCCACCAATCCTCAGCACGACGTGCTGTGCGTGGAA
CTGAGCTCCTTCCAATTGCACTTCACCGATTCGCTGGCACTTGACTGCGCGGCGATCACCAATATCGCCGACGATCACCT
CGACTGGCATGGCGGGCGCGAGAATTACGCCGCCGACAAATCCAAGGTGTTCCACAACGCCAAACACGCCATCGTCTACA
ATGCGCAGGACGCCAAAGTCAGCGAACTGGCGGCCGAGGCCCAAACCGCTGAAGGATGCCGCAAGGTGGGATTCACGCTC
GAGGCCCCCCAGGCCGGTCAGATCGGCATTGAAGATGGCTGGATTGTGGACAGGAGCGGCGTGGCCGGCGGCGCAGTGGG
GGAGCCGGTCAGACTGGCCGCCATCACCGATTTCACGCATCTGGCAGAGCCGGACGGCTCACTGTACCCGCATCTGGTGG
CCGATGCATTGACGGCATTGGCGTTGGTGCTGGGACTTGGCGCTGATCGCGATACCGCGCTGAAGGCGCTTACCTCGTTC
AAGCCGGGCGGCCATCGCATCGAAACCGTGGCTGAGGCCGTAGTCGAGGGCGGCTCTGTTCGTTTCGTGGACGATTCCAA
AGCCACCAACGGCCATGCGGCACGCGCCTCGCTGTCCAGCTTCCCGGCCAAGTCTGTGATCTGGATTGCCGGAGGTCTGG
CCAAGGGCAGTCGATTCGAGGACCTGGTCAAGGACCAGGCACATACCATCAAAGCGGCCGTCATCATCGGCAAAGACCAG
CAGCCGATGATCGAAGCGTTTGCCAGCCAAGCTCCGGACATCCCGGTGACCATCATCGATCCCGAAGACAACGATACCGT
CATGGATCGTGCCGTCGAAGCCTGCGGCACGTATGCCGCCGCCGGCGACATTGTGCTTATGGCTCCGGCCTGCGCCTCCA
TGGACCAGTTCAAGTCCTATGCGGATCGAGGCAATCGATTCGCCGCCGCAGCCAAGACGTGGAGCGAGGTGCATGGCCTC
CACTGA

Upstream 100 bases:

>100_bases
TTTCTACGGCGACTGGGTCACCCGCTCCGGCCTGCTGTTCAGCTAGCGGAAATCTGGGTGGATTATGCTCACCCACGGAA
TTCCTATATCAGGAGGAACA

Downstream 100 bases:

>100_bases
TATCCATAAGCGTAAGGCATACAGAGCCCCTGACAAGCCCGCACTCGAAATCACCGACTACACGGGTTGGCGAAGCTTGC
TCAACCCGCTATGGTGCTAC

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MQVADKTVVIAGLGVSGTSLAEVLRERGAHVIGVDERKPEADLRSFDDVDWDHVDYVMSSPVFNPRTPFVLEAQRRGIPV
MSEVEFAWQLRVNNERTGTPAPWIGITGTNGKTSTTEMTSEMLTACGLDAPTAGNIASGDMSMSLSRCATNPQHDVLCVE
LSSFQLHFTDSLALDCAAITNIADDHLDWHGGRENYAADKSKVFHNAKHAIVYNAQDAKVSELAAEAQTAEGCRKVGFTL
EAPQAGQIGIEDGWIVDRSGVAGGAVGEPVRLAAITDFTHLAEPDGSLYPHLVADALTALALVLGLGADRDTALKALTSF
KPGGHRIETVAEAVVEGGSVRFVDDSKATNGHAARASLSSFPAKSVIWIAGGLAKGSRFEDLVKDQAHTIKAAVIIGKDQ
QPMIEAFASQAPDIPVTIIDPEDNDTVMDRAVEACGTYAAAGDIVLMAPACASMDQFKSYADRGNRFAAAAKTWSEVHGL
H

Sequences:

>Translated_481_residues
MQVADKTVVIAGLGVSGTSLAEVLRERGAHVIGVDERKPEADLRSFDDVDWDHVDYVMSSPVFNPRTPFVLEAQRRGIPV
MSEVEFAWQLRVNNERTGTPAPWIGITGTNGKTSTTEMTSEMLTACGLDAPTAGNIASGDMSMSLSRCATNPQHDVLCVE
LSSFQLHFTDSLALDCAAITNIADDHLDWHGGRENYAADKSKVFHNAKHAIVYNAQDAKVSELAAEAQTAEGCRKVGFTL
EAPQAGQIGIEDGWIVDRSGVAGGAVGEPVRLAAITDFTHLAEPDGSLYPHLVADALTALALVLGLGADRDTALKALTSF
KPGGHRIETVAEAVVEGGSVRFVDDSKATNGHAARASLSSFPAKSVIWIAGGLAKGSRFEDLVKDQAHTIKAAVIIGKDQ
QPMIEAFASQAPDIPVTIIDPEDNDTVMDRAVEACGTYAAAGDIVLMAPACASMDQFKSYADRGNRFAAAAKTWSEVHGL
H
>Mature_481_residues
MQVADKTVVIAGLGVSGTSLAEVLRERGAHVIGVDERKPEADLRSFDDVDWDHVDYVMSSPVFNPRTPFVLEAQRRGIPV
MSEVEFAWQLRVNNERTGTPAPWIGITGTNGKTSTTEMTSEMLTACGLDAPTAGNIASGDMSMSLSRCATNPQHDVLCVE
LSSFQLHFTDSLALDCAAITNIADDHLDWHGGRENYAADKSKVFHNAKHAIVYNAQDAKVSELAAEAQTAEGCRKVGFTL
EAPQAGQIGIEDGWIVDRSGVAGGAVGEPVRLAAITDFTHLAEPDGSLYPHLVADALTALALVLGLGADRDTALKALTSF
KPGGHRIETVAEAVVEGGSVRFVDDSKATNGHAARASLSSFPAKSVIWIAGGLAKGSRFEDLVKDQAHTIKAAVIIGKDQ
QPMIEAFASQAPDIPVTIIDPEDNDTVMDRAVEACGTYAAAGDIVLMAPACASMDQFKSYADRGNRFAAAAKTWSEVHGL
H

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=484, Percent_Identity=29.9586776859504, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 51035; Mature: 51035

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVADKTVVIAGLGVSGTSLAEVLRERGAHVIGVDERKPEADLRSFDDVDWDHVDYVMSS
CCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHCCCCCCCHHHHHHHHC
PVFNPRTPFVLEAQRRGIPVMSEVEFAWQLRVNNERTGTPAPWIGITGTNGKTSTTEMTS
CCCCCCCCEEEEHHHCCCCCEECCEEEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHH
EMLTACGLDAPTAGNIASGDMSMSLSRCATNPQHDVLCVELSSFQLHFTDSLALDCAAIT
HHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCEEEEECCCHHHHHHHHH
NIADDHLDWHGGRENYAADKSKVFHNAKHAIVYNAQDAKVSELAAEAQTAEGCRKVGFTL
HHHHHCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEE
EAPQAGQIGIEDGWIVDRSGVAGGAVGEPVRLAAITDFTHLAEPDGSLYPHLVADALTAL
ECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECHHHHCCCCCCCCHHHHHHHHHHH
ALVLGLGADRDTALKALTSFKPGGHRIETVAEAVVEGGSVRFVDDSKATNGHAARASLSS
HHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
FPAKSVIWIAGGLAKGSRFEDLVKDQAHTIKAAVIIGKDQQPMIEAFASQAPDIPVTIID
CCCCEEEEEECCCCCCCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEC
PEDNDTVMDRAVEACGTYAAAGDIVLMAPACASMDQFKSYADRGNRFAAAAKTWSEVHGL
CCCCCHHHHHHHHHHCCHHCCCCEEEECCCCCCHHHHHHHHHCCCEEEHHHHHHHHHCCC
H
C
>Mature Secondary Structure
MQVADKTVVIAGLGVSGTSLAEVLRERGAHVIGVDERKPEADLRSFDDVDWDHVDYVMSS
CCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHCCCCCCCHHHHHHHHC
PVFNPRTPFVLEAQRRGIPVMSEVEFAWQLRVNNERTGTPAPWIGITGTNGKTSTTEMTS
CCCCCCCCEEEEHHHCCCCCEECCEEEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHH
EMLTACGLDAPTAGNIASGDMSMSLSRCATNPQHDVLCVELSSFQLHFTDSLALDCAAIT
HHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCEEEEECCCHHHHHHHHH
NIADDHLDWHGGRENYAADKSKVFHNAKHAIVYNAQDAKVSELAAEAQTAEGCRKVGFTL
HHHHHCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEE
EAPQAGQIGIEDGWIVDRSGVAGGAVGEPVRLAAITDFTHLAEPDGSLYPHLVADALTAL
ECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECHHHHCCCCCCCCHHHHHHHHHHH
ALVLGLGADRDTALKALTSFKPGGHRIETVAEAVVEGGSVRFVDDSKATNGHAARASLSS
HHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
FPAKSVIWIAGGLAKGSRFEDLVKDQAHTIKAAVIIGKDQQPMIEAFASQAPDIPVTIID
CCCCEEEEEECCCCCCCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEC
PEDNDTVMDRAVEACGTYAAAGDIVLMAPACASMDQFKSYADRGNRFAAAAKTWSEVHGL
CCCCCHHHHHHHHHHCCHHCCCCEEEECCCCCCHHHHHHHHHCCCEEEHHHHHHHHHCCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA