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The map label for this gene is murF [H]

Identifier: 213691746

GI number: 213691746

Start: 1004137

End: 1005588

Strand: Direct

Name: murF [H]

Synonym: Blon_0852

Alternate gene names: 213691746

Gene position: 1004137-1005588 (Clockwise)

Preceding gene: 213691745

Following gene: 213691747

Centisome position: 35.45

GC content: 61.78

Gene sequence:

>1452_bases
ATGGTTCCGATGAGCGTGGAGGAGATCGCCAAGGCCGTCGATGGTCGATTGATCGGCGACGCCGATTCCGAGTCTCGATC
TACCAATACGGTCAGTGACTCGCGGCAGGCCGGCCCCGGATCGGTGTTTGTGGCCATCCGAGGCGAGCATGTCGATGGGC
ATGACTTTGTGGCGAAAACCGCTGGACAAGGAGCCACGGTGGCCATCGTCGACCATGAGGTGGAGTCCGATGGCCTGCCG
CAGATCGTAGTGAAAGACACGGTCGACGCCCTTGGCGAGCTCGCCCGACACAACATCGTTCGCCGCCGCGAGCTGGACGG
CGACTTCGACATCATCGGATTGACCGGTTCGGTGGGCAAGACCACCACCAAGGATCTGCTCTCCTCCCTGCTTGCCACCG
TAGGCCCGACCGTGGCTCCGGTGGGATCGTTCAACAATGAGATCGGCCTGCCGCTCACTGCGTTGCGGGTCGATGAGCAC
ACCCGGTTCTTCGTGGCTGAGATGGGTGCCAGCCATATCGGCGAAATCGCGCGACTGACCCGTATCGCACCGCCGAACAC
GGCAATCGTATTGAAGGTCGGCGTCGCTCACCTTGGAGAATTCGGCTCCCGCGAACGCATCGCACAGGCGAAAAGCGAAA
TCGTGCAGGGGCTGCTTCCCGGAGGCACCGCGGTACTGAACGCCGATGACGAACACGTGGTTCCCATGGCCGGCTTGGCC
AGCGGCGATGTGCTGTGGTTCGGCTTGGGATCGTCGCAGGAGCCCGAGGTGCGGGCCATTGATGTCACTGCCGACCGGTC
CGATCATGCTGAATTCACGTTGGTCGATGCGGACGGCAACCATACGCCGGTGCATCTGGGCATTCCTGGACGTCACAACG
TGATGAACGCGTTGGCCGCCGCAACCGTGGCGATGCGTTACGGTATGGCACCCGAAACGGTGGCGCGGATTCTGGCCTCG
CAGCACACCATCAGCCCTCATCGCATGGCATTGTCCACGGTGAATCGCGAGGGGACTTCCTTCACACTGATCGATGATTC
CTTTAATGCCAATCCCGACTCCATGAAAGCCGGCCTGAACGGGTTGAAGGCTTGGAACTCAAATGACGGCAAGGATCCGT
TCCGCGTGGCGCTGCTGGGTGCCATGCTGGAACTCGGCGCCGATGAGACGGCATTGCACACCTCTATCGGCACGTATGCG
GCCGAATTGGGCATCGACGCGATCATCGCGGTCGGTTCCGCCCAAGACCAACATCTCGATGCACTGGCACAGGCTCTGGC
GGACGGTGCCCGTCAGAGCCCATCCGCGTCGGTGGATTGCGTACACGACATCGATGCGGCCGAACAGCTGGTCATCGACT
TGGCGCGCAACCATCCCGACGCTGTGGTACTGCTCAAGGGATCGCACGCGTCAGGCCTGAGCGCTTTGGCTGAACGCTGG
GCAAAGAACTGA

Upstream 100 bases:

>100_bases
GAGGAAAAACAACATACGCAGTGCCCTGCATGTGGCCAATAATCTCAATTAAGTCCGTGGATGCGGGACGAAGCGTAATC
GTAAAGGAGTTTGAAACACC

Downstream 100 bases:

>100_bases
CCTGATAACCATCCCAAGAAACCGACAAGAAAGAGAAGCTGCACGTGATTGCTCTGATCATTGGAATGCTGGTGTCGCTC
ATCGTCACCCTGGTCGGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MVPMSVEEIAKAVDGRLIGDADSESRSTNTVSDSRQAGPGSVFVAIRGEHVDGHDFVAKTAGQGATVAIVDHEVESDGLP
QIVVKDTVDALGELARHNIVRRRELDGDFDIIGLTGSVGKTTTKDLLSSLLATVGPTVAPVGSFNNEIGLPLTALRVDEH
TRFFVAEMGASHIGEIARLTRIAPPNTAIVLKVGVAHLGEFGSRERIAQAKSEIVQGLLPGGTAVLNADDEHVVPMAGLA
SGDVLWFGLGSSQEPEVRAIDVTADRSDHAEFTLVDADGNHTPVHLGIPGRHNVMNALAAATVAMRYGMAPETVARILAS
QHTISPHRMALSTVNREGTSFTLIDDSFNANPDSMKAGLNGLKAWNSNDGKDPFRVALLGAMLELGADETALHTSIGTYA
AELGIDAIIAVGSAQDQHLDALAQALADGARQSPSASVDCVHDIDAAEQLVIDLARNHPDAVVLLKGSHASGLSALAERW
AKN

Sequences:

>Translated_483_residues
MVPMSVEEIAKAVDGRLIGDADSESRSTNTVSDSRQAGPGSVFVAIRGEHVDGHDFVAKTAGQGATVAIVDHEVESDGLP
QIVVKDTVDALGELARHNIVRRRELDGDFDIIGLTGSVGKTTTKDLLSSLLATVGPTVAPVGSFNNEIGLPLTALRVDEH
TRFFVAEMGASHIGEIARLTRIAPPNTAIVLKVGVAHLGEFGSRERIAQAKSEIVQGLLPGGTAVLNADDEHVVPMAGLA
SGDVLWFGLGSSQEPEVRAIDVTADRSDHAEFTLVDADGNHTPVHLGIPGRHNVMNALAAATVAMRYGMAPETVARILAS
QHTISPHRMALSTVNREGTSFTLIDDSFNANPDSMKAGLNGLKAWNSNDGKDPFRVALLGAMLELGADETALHTSIGTYA
AELGIDAIIAVGSAQDQHLDALAQALADGARQSPSASVDCVHDIDAAEQLVIDLARNHPDAVVLLKGSHASGLSALAERW
AKN
>Mature_483_residues
MVPMSVEEIAKAVDGRLIGDADSESRSTNTVSDSRQAGPGSVFVAIRGEHVDGHDFVAKTAGQGATVAIVDHEVESDGLP
QIVVKDTVDALGELARHNIVRRRELDGDFDIIGLTGSVGKTTTKDLLSSLLATVGPTVAPVGSFNNEIGLPLTALRVDEH
TRFFVAEMGASHIGEIARLTRIAPPNTAIVLKVGVAHLGEFGSRERIAQAKSEIVQGLLPGGTAVLNADDEHVVPMAGLA
SGDVLWFGLGSSQEPEVRAIDVTADRSDHAEFTLVDADGNHTPVHLGIPGRHNVMNALAAATVAMRYGMAPETVARILAS
QHTISPHRMALSTVNREGTSFTLIDDSFNANPDSMKAGLNGLKAWNSNDGKDPFRVALLGAMLELGADETALHTSIGTYA
AELGIDAIIAVGSAQDQHLDALAQALADGARQSPSASVDCVHDIDAAEQLVIDLARNHPDAVVLLKGSHASGLSALAERW
AKN

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=491, Percent_Identity=32.3828920570265, Blast_Score=189, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50561; Mature: 50561

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPMSVEEIAKAVDGRLIGDADSESRSTNTVSDSRQAGPGSVFVAIRGEHVDGHDFVAKT
CCCCCHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHC
AGQGATVAIVDHEVESDGLPQIVVKDTVDALGELARHNIVRRRELDGDFDIIGLTGSVGK
CCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
TTTKDLLSSLLATVGPTVAPVGSFNNEIGLPLTALRVDEHTRFFVAEMGASHIGEIARLT
CHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEECCCCEEEHHHHCHHHHHHHHHHH
RIAPPNTAIVLKVGVAHLGEFGSRERIAQAKSEIVQGLLPGGTAVLNADDEHVVPMAGLA
HCCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCC
SGDVLWFGLGSSQEPEVRAIDVTADRSDHAEFTLVDADGNHTPVHLGIPGRHNVMNALAA
CCCEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHH
ATVAMRYGMAPETVARILASQHTISPHRMALSTVNREGTSFTLIDDSFNANPDSMKAGLN
HHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
GLKAWNSNDGKDPFRVALLGAMLELGADETALHTSIGTYAAELGIDAIIAVGSAQDQHLD
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHH
ALAQALADGARQSPSASVDCVHDIDAAEQLVIDLARNHPDAVVLLKGSHASGLSALAERW
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
AKN
CCC
>Mature Secondary Structure
MVPMSVEEIAKAVDGRLIGDADSESRSTNTVSDSRQAGPGSVFVAIRGEHVDGHDFVAKT
CCCCCHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHC
AGQGATVAIVDHEVESDGLPQIVVKDTVDALGELARHNIVRRRELDGDFDIIGLTGSVGK
CCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
TTTKDLLSSLLATVGPTVAPVGSFNNEIGLPLTALRVDEHTRFFVAEMGASHIGEIARLT
CHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEECCCCEEEHHHHCHHHHHHHHHHH
RIAPPNTAIVLKVGVAHLGEFGSRERIAQAKSEIVQGLLPGGTAVLNADDEHVVPMAGLA
HCCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCC
SGDVLWFGLGSSQEPEVRAIDVTADRSDHAEFTLVDADGNHTPVHLGIPGRHNVMNALAA
CCCEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHH
ATVAMRYGMAPETVARILASQHTISPHRMALSTVNREGTSFTLIDDSFNANPDSMKAGLN
HHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
GLKAWNSNDGKDPFRVALLGAMLELGADETALHTSIGTYAAELGIDAIIAVGSAQDQHLD
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHH
ALAQALADGARQSPSASVDCVHDIDAAEQLVIDLARNHPDAVVLLKGSHASGLSALAERW
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
AKN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9177243; 12060705 [H]