Click here to switch to the map view.

The map label for this gene is lexA [H]

Identifier: 213691736

GI number: 213691736

Start: 993660

End: 994385

Strand: Reverse

Name: lexA [H]

Synonym: Blon_0842

Alternate gene names: 213691736

Gene position: 994385-993660 (Counterclockwise)

Preceding gene: 213691739

Following gene: 213691735

Centisome position: 35.1

GC content: 58.95

Gene sequence:

>726_bases
GTGAGCACCATTCCCTTCTCCCCCAAGCAGAAGCCGGACGAGTCCACCCTAACCGACCGCCAGCGCAAAGTGTTGGACGC
CATTCGCACGCATATCGACGAACAAGGCTTCGCTCCCTCTTTCCGGGAAATCGGAAATGCCGCCGGGCTCAAAAGTCCGT
CTTCGGTCAAGCATCAGCTACAAGTTTTGGAAGACAAGGGCTTCATCCGCATGAACGCGAACAAGGGTCGCGCCATCGAG
GTGGTGGCAGGATCCGCCCCGAATCCCGAAAAGCCCTCCCAAGCCAGCGAAGAGGCAACCAGCACCAGTAATGTGGCGGA
GATATACCAATTCCCCGCCGAAGCCATTGCCGAATCACACGATGTGCCGCTCGTCGGCCGTATTGCCGCCGGTGTGCCAA
TTACCGCCGAACAGCATGTGGACGACGTCATGCGTCTGCCGGAACGGCTGACCGGCTCCGGCACCCTGTTCATGCTGGAA
GTCCACGGTGATTCCATGGTGGATGCCGCTATCTGCGATGGCGACTATGTGGTGGTGCGAGAACAGAATTCCGCCGTCAA
CGGAGATATCGTCGCCGCTCTGCTGGATGACGAGGCCACGGTGAAGACCTTCCGCAAGGAAAACGGCCATGTCTGGCTGA
TGCCGCACAACCCCGCATACTCGCCCATCGACGGCACACATGCAACGATTATGGGCAAGGTGGTCACCGTACTGCGCAAA
TTGTAG

Upstream 100 bases:

>100_bases
TGGCACAAATGTTCGACACGGTCAAGTCGAAACTCGAACAGATGTTTGATTTCACGCGAACAACACGTTATGCTGGTGGC
ATAACGAAAGGAGCCCTGAC

Downstream 100 bases:

>100_bases
CCTGTTCGCCACCAGCTGATCAGCGTGCGCTGAATCGCGCTGGTCGTGCTATAGTCGCACACAGCTTACGAAACCCCGGC
CTCGCGCCGGGGTTTCGTCG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSTIPFSPKQKPDESTLTDRQRKVLDAIRTHIDEQGFAPSFREIGNAAGLKSPSSVKHQLQVLEDKGFIRMNANKGRAIE
VVAGSAPNPEKPSQASEEATSTSNVAEIYQFPAEAIAESHDVPLVGRIAAGVPITAEQHVDDVMRLPERLTGSGTLFMLE
VHGDSMVDAAICDGDYVVVREQNSAVNGDIVAALLDDEATVKTFRKENGHVWLMPHNPAYSPIDGTHATIMGKVVTVLRK
L

Sequences:

>Translated_241_residues
MSTIPFSPKQKPDESTLTDRQRKVLDAIRTHIDEQGFAPSFREIGNAAGLKSPSSVKHQLQVLEDKGFIRMNANKGRAIE
VVAGSAPNPEKPSQASEEATSTSNVAEIYQFPAEAIAESHDVPLVGRIAAGVPITAEQHVDDVMRLPERLTGSGTLFMLE
VHGDSMVDAAICDGDYVVVREQNSAVNGDIVAALLDDEATVKTFRKENGHVWLMPHNPAYSPIDGTHATIMGKVVTVLRK
L
>Mature_240_residues
STIPFSPKQKPDESTLTDRQRKVLDAIRTHIDEQGFAPSFREIGNAAGLKSPSSVKHQLQVLEDKGFIRMNANKGRAIEV
VAGSAPNPEKPSQASEEATSTSNVAEIYQFPAEAIAESHDVPLVGRIAAGVPITAEQHVDDVMRLPERLTGSGTLFMLEV
HGDSMVDAAICDGDYVVVREQNSAVNGDIVAALLDDEATVKTFRKENGHVWLMPHNPAYSPIDGTHATIMGKVVTVLRKL

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=227, Percent_Identity=33.0396475770925, Blast_Score=114, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 26076; Mature: 25945

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIPFSPKQKPDESTLTDRQRKVLDAIRTHIDEQGFAPSFREIGNAAGLKSPSSVKHQL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHH
QVLEDKGFIRMNANKGRAIEVVAGSAPNPEKPSQASEEATSTSNVAEIYQFPAEAIAESH
HHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHCHHHHHHHHHCCHHHHHCCC
DVPLVGRIAAGVPITAEQHVDDVMRLPERLTGSGTLFMLEVHGDSMVDAAICDGDYVVVR
CCCEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCEEEEE
EQNSAVNGDIVAALLDDEATVKTFRKENGHVWLMPHNPAYSPIDGTHATIMGKVVTVLRK
CCCCCCCCCEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
L
C
>Mature Secondary Structure 
STIPFSPKQKPDESTLTDRQRKVLDAIRTHIDEQGFAPSFREIGNAAGLKSPSSVKHQL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHH
QVLEDKGFIRMNANKGRAIEVVAGSAPNPEKPSQASEEATSTSNVAEIYQFPAEAIAESH
HHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHCHHHHHHHHHCCHHHHHCCC
DVPLVGRIAAGVPITAEQHVDDVMRLPERLTGSGTLFMLEVHGDSMVDAAICDGDYVVVR
CCCEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCEEEEE
EQNSAVNGDIVAALLDDEATVKTFRKENGHVWLMPHNPAYSPIDGTHATIMGKVVTVLRK
CCCCCCCCCEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA