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The map label for this gene is uvrD [H]

Identifier: 213691669

GI number: 213691669

Start: 895596

End: 897170

Strand: Direct

Name: uvrD [H]

Synonym: Blon_0774

Alternate gene names: 213691669

Gene position: 895596-897170 (Clockwise)

Preceding gene: 213691666

Following gene: 213691671

Centisome position: 31.62

GC content: 62.41

Gene sequence:

>1575_bases
ATGGAACAGGCGCAGGGGATTCTTGAGGGGCTCGATGAGCGGCAGCGTACCGCCGCGACTACGTTGCAAGGGCCGGTGCG
CATCATCGCCGGCGCAGGTGCCGGTAAAACCCGCGCGGTCACGCGGCGGATCGCCTATGCCTGCGCCACCGGGGCATGGA
AGCCGGAGCGGGTGCTTGCCGTCACCTTTTCGGTAAAAGCGGCGGCGGAAATGCGCGCACGGTTGGGCCAGCTCGGCGTA
GGGGGAGGTACAGGACGCTCCGGTTCGGGTGCGGGAACTGCCGGAGTCACGGCCGCGACCTTCCACTCAGCGGCATTGCA
TCAGCTCAACAGCGTCTGGTCAGACATATGCGAGGCTCCATTCCCGCATGTGATCGAAGATCAGCGTGACGTCGTCTCCC
GCGCGATCATCCGCGCCACCGCACGTACCGATGCCGATCCGCTGACCGTTCGCGATGTGCTCGCCGAAATCAATTGGGCC
AAAATCTCGTTGGTGGCGGTGGAAGATTATGCACGGACCTGTGCCATCGCCCATAGGCAACCGCCCGCCGGACTCGACAC
CGCTCGGTTCGCCGACGTCTACAGCGCCTACGAGCAGGAAAAGACCTCGCGTGGCGAAATCGATTTTGACGATATTCTGC
TGCTCGTATGCCATATCATGGATGATTTTCCCGAAGCCGCCGCCCAAATTCGTTCCACCATCGGCTGGCTCACCGTCGAC
GAGTATCAGGACGTCTCGCCCCTGCAACACAGGCTGCTGACCCTGTGGCTGGGCGGGGGAGGACCTGACGGCAAGAGCGC
GATGAACCGCAACGTATGCGTCGTGGGCGATCCGGCCCAGACCATCTACTCGTTTGCCGGTGCCTCCAGCTGGGATCTGC
TGCGTTTCGCCGATGAATTCGGGCCGCTCGCCGCCGATGTCAATCTCAACACCGACTATCGTTCGACGCCGGAAGTCGTG
AACCTCGCCAACCGGGTGCTTGCGGCCGCGCCGAACCGTGAGGATTACCTGAAACTGGTTTCCGCCCGCGAATCCGGCCC
GCGCGTTACCCGAACCGCTTATGAGAGCGATATGAAAGAGGCGCAGGGCGTGGCGGCACGCATCGCCCGGTTGGTCGCCA
ACGGGGCCAAGCCGTCCGATTGCGCGATCCTCACCCGCATCAACGCCCAACAGCCGGTATTCGGTGCCGCCTTGCGCGCT
GCAAGACTGAAATACCGCGTCCGCAAGGATTCGGGATGGCAATCATCCGCGCTCGCCGACGATGCCGCGTCCAAACGCGC
ACTATTGGAGGCGATGGGTTTGGACGCCACCGGACTTGCCGCAAACGACGATCCCGGCGTTACGATTTCCACGATCCACG
CATCCAAGGGTCTGGAATTCAAGCACGTGTTCCTGATCGGCTGTTCGGAAGGGCTCTTGCCGTACGGTTCGCCGGAAACG
GGGGATGCGCTGGAAGAGGAACGCCGGCTTATGTATGTGGGCATCACCCGAGCTGAGGATTCGCTGCATCTGTCGTATGC
GCGGACCAAGGATGGTTATGGAGCGCAACACAGACGCCCATCACGATTTTTGTGA

Upstream 100 bases:

>100_bases
GACACGCAAAACGCATGATTCTGGCGATGTTTTGTCGTGAGCCCGATTCAGCCATAGGCGTGAGTCGTGCCGTGAGCGCG
AATCGTGCCACAATGGACGC

Downstream 100 bases:

>100_bases
CGGGCGTCGTTCATGCATACTGAATGCTCCTGAACATTCAGGAGCGGGAATTGGCTGAAGGCCGGTTGACTATGCCTGCG
TATCCTCCGAGTTGCCGTTT

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MEQAQGILEGLDERQRTAATTLQGPVRIIAGAGAGKTRAVTRRIAYACATGAWKPERVLAVTFSVKAAAEMRARLGQLGV
GGGTGRSGSGAGTAGVTAATFHSAALHQLNSVWSDICEAPFPHVIEDQRDVVSRAIIRATARTDADPLTVRDVLAEINWA
KISLVAVEDYARTCAIAHRQPPAGLDTARFADVYSAYEQEKTSRGEIDFDDILLLVCHIMDDFPEAAAQIRSTIGWLTVD
EYQDVSPLQHRLLTLWLGGGGPDGKSAMNRNVCVVGDPAQTIYSFAGASSWDLLRFADEFGPLAADVNLNTDYRSTPEVV
NLANRVLAAAPNREDYLKLVSARESGPRVTRTAYESDMKEAQGVAARIARLVANGAKPSDCAILTRINAQQPVFGAALRA
ARLKYRVRKDSGWQSSALADDAASKRALLEAMGLDATGLAANDDPGVTISTIHASKGLEFKHVFLIGCSEGLLPYGSPET
GDALEEERRLMYVGITRAEDSLHLSYARTKDGYGAQHRRPSRFL

Sequences:

>Translated_524_residues
MEQAQGILEGLDERQRTAATTLQGPVRIIAGAGAGKTRAVTRRIAYACATGAWKPERVLAVTFSVKAAAEMRARLGQLGV
GGGTGRSGSGAGTAGVTAATFHSAALHQLNSVWSDICEAPFPHVIEDQRDVVSRAIIRATARTDADPLTVRDVLAEINWA
KISLVAVEDYARTCAIAHRQPPAGLDTARFADVYSAYEQEKTSRGEIDFDDILLLVCHIMDDFPEAAAQIRSTIGWLTVD
EYQDVSPLQHRLLTLWLGGGGPDGKSAMNRNVCVVGDPAQTIYSFAGASSWDLLRFADEFGPLAADVNLNTDYRSTPEVV
NLANRVLAAAPNREDYLKLVSARESGPRVTRTAYESDMKEAQGVAARIARLVANGAKPSDCAILTRINAQQPVFGAALRA
ARLKYRVRKDSGWQSSALADDAASKRALLEAMGLDATGLAANDDPGVTISTIHASKGLEFKHVFLIGCSEGLLPYGSPET
GDALEEERRLMYVGITRAEDSLHLSYARTKDGYGAQHRRPSRFL
>Mature_524_residues
MEQAQGILEGLDERQRTAATTLQGPVRIIAGAGAGKTRAVTRRIAYACATGAWKPERVLAVTFSVKAAAEMRARLGQLGV
GGGTGRSGSGAGTAGVTAATFHSAALHQLNSVWSDICEAPFPHVIEDQRDVVSRAIIRATARTDADPLTVRDVLAEINWA
KISLVAVEDYARTCAIAHRQPPAGLDTARFADVYSAYEQEKTSRGEIDFDDILLLVCHIMDDFPEAAAQIRSTIGWLTVD
EYQDVSPLQHRLLTLWLGGGGPDGKSAMNRNVCVVGDPAQTIYSFAGASSWDLLRFADEFGPLAADVNLNTDYRSTPEVV
NLANRVLAAAPNREDYLKLVSARESGPRVTRTAYESDMKEAQGVAARIARLVANGAKPSDCAILTRINAQQPVFGAALRA
ARLKYRVRKDSGWQSSALADDAASKRALLEAMGLDATGLAANDDPGVTISTIHASKGLEFKHVFLIGCSEGLLPYGSPET
GDALEEERRLMYVGITRAEDSLHLSYARTKDGYGAQHRRPSRFL

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=406, Percent_Identity=29.8029556650246, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI48994965, Length=400, Percent_Identity=28, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789183, Length=145, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322369, Length=484, Percent_Identity=23.9669421487603, Blast_Score=116, Evalue=8e-27,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR002121
- InterPro:   IPR014016
- InterPro:   IPR010997 [H]

Pfam domain/function: PF00570 HRDC; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 56336; Mature: 56336

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQAQGILEGLDERQRTAATTLQGPVRIIAGAGAGKTRAVTRRIAYACATGAWKPERVLA
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEE
VTFSVKAAAEMRARLGQLGVGGGTGRSGSGAGTAGVTAATFHSAALHQLNSVWSDICEAP
EEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
FPHVIEDQRDVVSRAIIRATARTDADPLTVRDVLAEINWAKISLVAVEDYARTCAIAHRQ
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEEEEEHHHHHHHHHHCCC
PPAGLDTARFADVYSAYEQEKTSRGEIDFDDILLLVCHIMDDFPEAAAQIRSTIGWLTVD
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEC
EYQDVSPLQHRLLTLWLGGGGPDGKSAMNRNVCVVGDPAQTIYSFAGASSWDLLRFADEF
CCCCCCHHHHEEEEEEECCCCCCCHHHCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHH
GPLAADVNLNTDYRSTPEVVNLANRVLAAAPNREDYLKLVSARESGPRVTRTAYESDMKE
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AQGVAARIARLVANGAKPSDCAILTRINAQQPVFGAALRAARLKYRVRKDSGWQSSALAD
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHH
DAASKRALLEAMGLDATGLAANDDPGVTISTIHASKGLEFKHVFLIGCSEGLLPYGSPET
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCC
GDALEEERRLMYVGITRAEDSLHLSYARTKDGYGAQHRRPSRFL
CCHHHHCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEQAQGILEGLDERQRTAATTLQGPVRIIAGAGAGKTRAVTRRIAYACATGAWKPERVLA
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEE
VTFSVKAAAEMRARLGQLGVGGGTGRSGSGAGTAGVTAATFHSAALHQLNSVWSDICEAP
EEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
FPHVIEDQRDVVSRAIIRATARTDADPLTVRDVLAEINWAKISLVAVEDYARTCAIAHRQ
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEEEEEHHHHHHHHHHCCC
PPAGLDTARFADVYSAYEQEKTSRGEIDFDDILLLVCHIMDDFPEAAAQIRSTIGWLTVD
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEC
EYQDVSPLQHRLLTLWLGGGGPDGKSAMNRNVCVVGDPAQTIYSFAGASSWDLLRFADEF
CCCCCCHHHHEEEEEEECCCCCCCHHHCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHH
GPLAADVNLNTDYRSTPEVVNLANRVLAAAPNREDYLKLVSARESGPRVTRTAYESDMKE
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AQGVAARIARLVANGAKPSDCAILTRINAQQPVFGAALRAARLKYRVRKDSGWQSSALAD
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHH
DAASKRALLEAMGLDATGLAANDDPGVTISTIHASKGLEFKHVFLIGCSEGLLPYGSPET
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCC
GDALEEERRLMYVGITRAEDSLHLSYARTKDGYGAQHRRPSRFL
CCHHHHCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]