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The map label for this gene is dapF [H]

Identifier: 213691664

GI number: 213691664

Start: 890694

End: 891587

Strand: Direct

Name: dapF [H]

Synonym: Blon_0769

Alternate gene names: 213691664

Gene position: 890694-891587 (Clockwise)

Preceding gene: 213691661

Following gene: 213691666

Centisome position: 31.44

GC content: 61.07

Gene sequence:

>894_bases
ATGAGTCTTCCGCAACGAGTAACCAAGACACACGCAACCGGCAATGATTTCGTGGTGTACCTCGACAAGTCCGGCAAATT
CGAGCCCACCGCCGATGAGGTTCGATTCCTGTGCGACCGTCACTTCGGCATTGGTGGCGATGGCCTGATTCGTCTGACCC
GTCCTGAGTTCGTGTCCGACCTAAGCGATGACCAGGTTTCGGCTGTGCTAGCCGGGGGCGCGAAATGGTTCATGGACTAT
CGCAACGCGGATGGATCGTTGGCGGAGATGTGCGGCAATGGTACACGCGCCATCACCTTGTTCGCCCAACGCGAGGGTGT
GGCCGACTCCACCGAGCCGTTCCGCCTCGGCACCCGTGCCGGCGTCAAGATTCTCACTCCACGCAGCAACACGAAGCCAT
ACGGAGCCAACGTCTTCCAGATCGAAATGGGGGAGTGGAAGGTCGGCGAAACCGATGCCTACGAGGTCACCATCCCCGGC
ACTGAGGGCTCGGCGCGCGGTACATTCGTGGATATGGGCAACCCGCATGTGGTCGCGGTGATCGAGGATGCCTTCGCGTC
CCTGCCAGTGGTTGAATCACTGGATTTGGTCACCAAGCCCGTGGTGGCTCCCGTCATCGAATCCGATCAGAACGTGGAAT
TCGTGCGGATCGACGAGATCGACCAGAACACCGGCGTGGGCGAGGCCACAATGCGCGTCAACGAGCGTGGCTGCGGCGAG
ACCCTATCCTGTGGTACCGGCCTGTGCGCCACAGGCATTGTGCTGCGGGCCAAGACCGGCATCAGCCACTGGGACATCAC
CGTACGTGGCGGCACCCTGCGCGTGGACGTCACCGACAGCGACGTAAAGCTGACCGGAGCAGCCACGCTGGTCGCCGATA
TCGATTTGTTGTAA

Upstream 100 bases:

>100_bases
TCCTCCCTATAGCCATGCCTGCAACCGTATGTTGCCGCGAACCGAACATAAAGCCGATTTTCAGCGGTGTTGTTTCGCTC
CCTAGAGTACTCTGAAAGCC

Downstream 100 bases:

>100_bases
AGGAATCAGGCAGCGAACAGGGCCGCGCCTTCGGTGACGATGATGAGTACCACGATCGCCACGATCCACAGTATGTCGAC
CATGAGGTCGATGTTGAGGC

Product: Diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSLPQRVTKTHATGNDFVVYLDKSGKFEPTADEVRFLCDRHFGIGGDGLIRLTRPEFVSDLSDDQVSAVLAGGAKWFMDY
RNADGSLAEMCGNGTRAITLFAQREGVADSTEPFRLGTRAGVKILTPRSNTKPYGANVFQIEMGEWKVGETDAYEVTIPG
TEGSARGTFVDMGNPHVVAVIEDAFASLPVVESLDLVTKPVVAPVIESDQNVEFVRIDEIDQNTGVGEATMRVNERGCGE
TLSCGTGLCATGIVLRAKTGISHWDITVRGGTLRVDVTDSDVKLTGAATLVADIDLL

Sequences:

>Translated_297_residues
MSLPQRVTKTHATGNDFVVYLDKSGKFEPTADEVRFLCDRHFGIGGDGLIRLTRPEFVSDLSDDQVSAVLAGGAKWFMDY
RNADGSLAEMCGNGTRAITLFAQREGVADSTEPFRLGTRAGVKILTPRSNTKPYGANVFQIEMGEWKVGETDAYEVTIPG
TEGSARGTFVDMGNPHVVAVIEDAFASLPVVESLDLVTKPVVAPVIESDQNVEFVRIDEIDQNTGVGEATMRVNERGCGE
TLSCGTGLCATGIVLRAKTGISHWDITVRGGTLRVDVTDSDVKLTGAATLVADIDLL
>Mature_296_residues
SLPQRVTKTHATGNDFVVYLDKSGKFEPTADEVRFLCDRHFGIGGDGLIRLTRPEFVSDLSDDQVSAVLAGGAKWFMDYR
NADGSLAEMCGNGTRAITLFAQREGVADSTEPFRLGTRAGVKILTPRSNTKPYGANVFQIEMGEWKVGETDAYEVTIPGT
EGSARGTFVDMGNPHVVAVIEDAFASLPVVESLDLVTKPVVAPVIESDQNVEFVRIDEIDQNTGVGEATMRVNERGCGET
LSCGTGLCATGIVLRAKTGISHWDITVRGGTLRVDVTDSDVKLTGAATLVADIDLL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=313, Percent_Identity=25.5591054313099, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31833; Mature: 31702

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPQRVTKTHATGNDFVVYLDKSGKFEPTADEVRFLCDRHFGIGGDGLIRLTRPEFVSD
CCCCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCHHHHC
LSDDQVSAVLAGGAKWFMDYRNADGSLAEMCGNGTRAITLFAQREGVADSTEPFRLGTRA
CCCCCEEEEEECCCEEEEEECCCCCHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEECCC
GVKILTPRSNTKPYGANVFQIEMGEWKVGETDAYEVTIPGTEGSARGTFVDMGNPHVVAV
CEEEECCCCCCCCCCCEEEEEECCCEECCCCCEEEEEECCCCCCCCEEEEECCCCEEEEE
IEDAFASLPVVESLDLVTKPVVAPVIESDQNVEFVRIDEIDQNTGVGEATMRVNERGCGE
EECHHHCCCHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCC
TLSCGTGLCATGIVLRAKTGISHWDITVRGGTLRVDVTDSDVKLTGAATLVADIDLL
CCCCCCCHHHHCEEEEEECCCCEEEEEEECCEEEEEEECCCEEEEEEEEEEEEEECC
>Mature Secondary Structure 
SLPQRVTKTHATGNDFVVYLDKSGKFEPTADEVRFLCDRHFGIGGDGLIRLTRPEFVSD
CCCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCHHHHC
LSDDQVSAVLAGGAKWFMDYRNADGSLAEMCGNGTRAITLFAQREGVADSTEPFRLGTRA
CCCCCEEEEEECCCEEEEEECCCCCHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEECCC
GVKILTPRSNTKPYGANVFQIEMGEWKVGETDAYEVTIPGTEGSARGTFVDMGNPHVVAV
CEEEECCCCCCCCCCCEEEEEECCCEECCCCCEEEEEECCCCCCCCEEEEECCCCEEEEE
IEDAFASLPVVESLDLVTKPVVAPVIESDQNVEFVRIDEIDQNTGVGEATMRVNERGCGE
EECHHHCCCHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCC
TLSCGTGLCATGIVLRAKTGISHWDITVRGGTLRVDVTDSDVKLTGAATLVADIDLL
CCCCCCCHHHHCEEEEEECCCCEEEEEEECCEEEEEEECCCEEEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA