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The map label for this gene is tcyA [H]

Identifier: 213691656

GI number: 213691656

Start: 882488

End: 883300

Strand: Direct

Name: tcyA [H]

Synonym: Blon_0760

Alternate gene names: 213691656

Gene position: 882488-883300 (Clockwise)

Preceding gene: 213691655

Following gene: 213691657

Centisome position: 31.15

GC content: 55.35

Gene sequence:

>813_bases
ATGTTCAATAATCAAGTGATTCGCAAGGCTGCGGCTTTGCTGTCCGGTGTCGCCGTGCTGGTTTCGGTTGCCGCATGCGG
TTCGGCTTCCGCTTCCAGCTCCAGTGCATTGGAGCAGGTCAAGCAGTCCGGCAAAATCGTGTTCGCCACTGAGGGCACTT
ATGCTCCGTTCAGTTACCATGACTCCAAAACCAATGAGCTGACCGGTTACGATGTCGAGGTGGCCAAGGCCATTGCCAAG
GAGGTCGGTATCAAGGCCGAGTTTGCCGAGGGCAGCTTCGACTCCCTGCTTGCCGGTGTGGATGCCAAGAAGTACGATAC
GGTTGCCGATCAGATTTCCGCCACCGACGAGCGCAAGGCCAAGTATGATTTCTCCGAACCATACACCTACTCCTACGGTG
TCGTCATTACCTCCAAGGAGAACCCGAAGAACGTCACTTCGTTCGATGACGTCAAGGGGCTTCGCGCCGCTGAAACCGGT
ACCTCCAACTGGAACAAGACCGCTCAGGAGAAGGGCGCTACTATCGTTCAGGTTAACGATTTTGGTCAGCAGGTCGATGC
CATCACGTCCGGTCGTGCAGATGTGTCATTGAACGATGGCCTTGCTGCGCTCGACTACCTGAAGCAGAAGCCGGATGCGG
ACATCAAGATTGCCGCCAAGTCCGAGAAGACTCCGGCCGCCTACCTGCCGTTCCGCAAGGGCAGCGATGACTTGGTCAAG
GAAGTCAACAAAGCCATTGTCAAGCTGCAGAAGGATGGCACATTGACCAAGATCTCCGAAAAGTACTTTGGCGAGGACTT
CTCTCAGGAGTGA

Upstream 100 bases:

>100_bases
CTGTCTTCAACCAACAATGTGCTGATGATTCCGGTGACTCGGTAGAGGCGTTTCACGGCTGTTGTAATTAATTGTGACAA
TATCAAGAGAGGGGTTTGTC

Downstream 100 bases:

>100_bases
CATGTTTCCCGGCTCTCCTGTAATTAGGGGAGCCGGGAAATATGAGCTGGCCTCGTGAAGATTACGAGCAGGTGCAACAT
ATACCACACAACAAAGGATG

Product: extracellular solute-binding protein, family 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MFNNQVIRKAAALLSGVAVLVSVAACGSASASSSSALEQVKQSGKIVFATEGTYAPFSYHDSKTNELTGYDVEVAKAIAK
EVGIKAEFAEGSFDSLLAGVDAKKYDTVADQISATDERKAKYDFSEPYTYSYGVVITSKENPKNVTSFDDVKGLRAAETG
TSNWNKTAQEKGATIVQVNDFGQQVDAITSGRADVSLNDGLAALDYLKQKPDADIKIAAKSEKTPAAYLPFRKGSDDLVK
EVNKAIVKLQKDGTLTKISEKYFGEDFSQE

Sequences:

>Translated_270_residues
MFNNQVIRKAAALLSGVAVLVSVAACGSASASSSSALEQVKQSGKIVFATEGTYAPFSYHDSKTNELTGYDVEVAKAIAK
EVGIKAEFAEGSFDSLLAGVDAKKYDTVADQISATDERKAKYDFSEPYTYSYGVVITSKENPKNVTSFDDVKGLRAAETG
TSNWNKTAQEKGATIVQVNDFGQQVDAITSGRADVSLNDGLAALDYLKQKPDADIKIAAKSEKTPAAYLPFRKGSDDLVK
EVNKAIVKLQKDGTLTKISEKYFGEDFSQE
>Mature_270_residues
MFNNQVIRKAAALLSGVAVLVSVAACGSASASSSSALEQVKQSGKIVFATEGTYAPFSYHDSKTNELTGYDVEVAKAIAK
EVGIKAEFAEGSFDSLLAGVDAKKYDTVADQISATDERKAKYDFSEPYTYSYGVVITSKENPKNVTSFDDVKGLRAAETG
TSNWNKTAQEKGATIVQVNDFGQQVDAITSGRADVSLNDGLAALDYLKQKPDADIKIAAKSEKTPAAYLPFRKGSDDLVK
EVNKAIVKLQKDGTLTKISEKYFGEDFSQE

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=258, Percent_Identity=35.2713178294574, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787031, Length=246, Percent_Identity=30.8943089430894, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788649, Length=246, Percent_Identity=30.0813008130081, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787085, Length=229, Percent_Identity=28.82096069869, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=28.8793103448276, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28963; Mature: 28963

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNNQVIRKAAALLSGVAVLVSVAACGSASASSSSALEQVKQSGKIVFATEGTYAPFSYH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCC
DSKTNELTGYDVEVAKAIAKEVGIKAEFAEGSFDSLLAGVDAKKYDTVADQISATDERKA
CCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHC
KYDFSEPYTYSYGVVITSKENPKNVTSFDDVKGLRAAETGTSNWNKTAQEKGATIVQVND
CCCCCCCCEEEEEEEEECCCCCCCCCCHHHHCCCCHHCCCCCCCCHHHHHCCCEEEEECC
FGQQVDAITSGRADVSLNDGLAALDYLKQKPDADIKIAAKSEKTPAAYLPFRKGSDDLVK
HHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCCCCHHHHH
EVNKAIVKLQKDGTLTKISEKYFGEDFSQE
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFNNQVIRKAAALLSGVAVLVSVAACGSASASSSSALEQVKQSGKIVFATEGTYAPFSYH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCC
DSKTNELTGYDVEVAKAIAKEVGIKAEFAEGSFDSLLAGVDAKKYDTVADQISATDERKA
CCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHC
KYDFSEPYTYSYGVVITSKENPKNVTSFDDVKGLRAAETGTSNWNKTAQEKGATIVQVND
CCCCCCCCEEEEEEEEECCCCCCCCCCHHHHCCCCHHCCCCCCCCHHHHHCCCEEEEECC
FGQQVDAITSGRADVSLNDGLAALDYLKQKPDADIKIAAKSEKTPAAYLPFRKGSDDLVK
HHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCCCCHHHHH
EVNKAIVKLQKDGTLTKISEKYFGEDFSQE
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]