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The map label for this gene is gluB [H]

Identifier: 213691606

GI number: 213691606

Start: 817560

End: 818399

Strand: Direct

Name: gluB [H]

Synonym: Blon_0710

Alternate gene names: 213691606

Gene position: 817560-818399 (Clockwise)

Preceding gene: 213691605

Following gene: 213691607

Centisome position: 28.86

GC content: 61.9

Gene sequence:

>840_bases
ATGACATTCACCAATACGTTCAAGCGGAAGGCTTGCCGCGTGATCGCCGCGCTCGCCGCCGTGGCCTGCACAATGTCGCT
GGCCGCCTGCGGCGCGGACGAAACCGGCAAGATCCGCATCGGCATCAAATTCGACCAGCCCGGCCTGGGCTTCAAGAAGT
CCGGCACCTACGTGGGCTTCGACGTGGACGTGGCCAAGTACATCGCCAAGAAACTCGGCTATTCCGAAGACCAGATCATC
TGGAAGGAAGCCCCCTCCAAGCAGCGTGAGGCCATGATTCAGAACGGCGACGTGGACATGATCCTCGCCACCTACTCCAT
CACCGACGAGCGCAAGAAGGCAGTCTCCTTCGCCGGCCCGTACTTCGTGGCCGGACAGGATCTGCTCGTGCGCAAGGACG
ACAACTCCATCAACGGCCCCGAAGACCTGAACGGTAAGCGCCTATGCTCCGTCACCGGTTCGACCTCCGCCGCCACCGTC
AAGGAGAAGTTCGCCTCCGAAGTCCAGCTTATGGAACAGCCCGGTTACGCCGAATGCGCCACCGCCCTGTTCTCCGGCAT
CGTGGACGCGGTAACCACCGACGACATCATCCTGGCCGGCCTGGCCTCTGCCTCGCGCGGCAAGCTCAAGGTGGTCGGCA
AACCGTTCACGCAGGAACACTACGGCGTGGGCATCAAGAAGGGCGACACGCAGCTGGCCACCAAAATCAACAACGCCATC
GTGGACATGATTCAGGACGGCTCGTGGGAGAACGCCATCAGCGATAACACCAAGGGCGTCGACTACACGCCCGACGTACG
CTACAACCCGCCCACCCCGAACGAAGGAGAGGACCGATGA

Upstream 100 bases:

>100_bases
GAGAACACGCCCGACGAGTTCTTCGAGCATCCGCAGACCGACCGTGCCAAGGACTTCCTCTCGAAGATCCTCACCCACTG
AAAGACGGGTACGCAATCCA

Downstream 100 bases:

>100_bases
ACGGATTCATGGAACTTCTCGGCCACTATGACGTGTTCGGCGCCTTTCTGGTCAACCTCGAGCTGACCTTGTGGTCGGCC
TTGTTCTCCACGGTCATCGG

Product: extracellular solute-binding protein, family 3

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTFTNTFKRKACRVIAALAAVACTMSLAACGADETGKIRIGIKFDQPGLGFKKSGTYVGFDVDVAKYIAKKLGYSEDQII
WKEAPSKQREAMIQNGDVDMILATYSITDERKKAVSFAGPYFVAGQDLLVRKDDNSINGPEDLNGKRLCSVTGSTSAATV
KEKFASEVQLMEQPGYAECATALFSGIVDAVTTDDIILAGLASASRGKLKVVGKPFTQEHYGVGIKKGDTQLATKINNAI
VDMIQDGSWENAISDNTKGVDYTPDVRYNPPTPNEGEDR

Sequences:

>Translated_279_residues
MTFTNTFKRKACRVIAALAAVACTMSLAACGADETGKIRIGIKFDQPGLGFKKSGTYVGFDVDVAKYIAKKLGYSEDQII
WKEAPSKQREAMIQNGDVDMILATYSITDERKKAVSFAGPYFVAGQDLLVRKDDNSINGPEDLNGKRLCSVTGSTSAATV
KEKFASEVQLMEQPGYAECATALFSGIVDAVTTDDIILAGLASASRGKLKVVGKPFTQEHYGVGIKKGDTQLATKINNAI
VDMIQDGSWENAISDNTKGVDYTPDVRYNPPTPNEGEDR
>Mature_278_residues
TFTNTFKRKACRVIAALAAVACTMSLAACGADETGKIRIGIKFDQPGLGFKKSGTYVGFDVDVAKYIAKKLGYSEDQIIW
KEAPSKQREAMIQNGDVDMILATYSITDERKKAVSFAGPYFVAGQDLLVRKDDNSINGPEDLNGKRLCSVTGSTSAATVK
EKFASEVQLMEQPGYAECATALFSGIVDAVTTDDIILAGLASASRGKLKVVGKPFTQEHYGVGIKKGDTQLATKINNAIV
DMIQDGSWENAISDNTKGVDYTPDVRYNPPTPNEGEDR

Specific function: Part of the binding-protein-dependent transport system for glutamate [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=236, Percent_Identity=28.8135593220339, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788228, Length=269, Percent_Identity=28.996282527881, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788648, Length=204, Percent_Identity=26.4705882352941, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=25.4980079681275, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788649, Length=210, Percent_Identity=24.7619047619048, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786876, Length=202, Percent_Identity=26.2376237623762, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787088, Length=242, Percent_Identity=25.2066115702479, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30060; Mature: 29929

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTNTFKRKACRVIAALAAVACTMSLAACGADETGKIRIGIKFDQPGLGFKKSGTYVGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCEEEE
DVDVAKYIAKKLGYSEDQIIWKEAPSKQREAMIQNGDVDMILATYSITDERKKAVSFAGP
CHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCC
YFVAGQDLLVRKDDNSINGPEDLNGKRLCSVTGSTSAATVKEKFASEVQLMEQPGYAECA
EEEECCEEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHH
TALFSGIVDAVTTDDIILAGLASASRGKLKVVGKPFTQEHYGVGIKKGDTQLATKINNAI
HHHHHHHHHHHCCCHHHEEECCCCCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHH
VDMIQDGSWENAISDNTKGVDYTPDVRYNPPTPNEGEDR
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFTNTFKRKACRVIAALAAVACTMSLAACGADETGKIRIGIKFDQPGLGFKKSGTYVGF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCEEEE
DVDVAKYIAKKLGYSEDQIIWKEAPSKQREAMIQNGDVDMILATYSITDERKKAVSFAGP
CHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCC
YFVAGQDLLVRKDDNSINGPEDLNGKRLCSVTGSTSAATVKEKFASEVQLMEQPGYAECA
EEEECCEEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHH
TALFSGIVDAVTTDDIILAGLASASRGKLKVVGKPFTQEHYGVGIKKGDTQLATKINNAI
HHHHHHHHHHHCCCHHHEEECCCCCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHH
VDMIQDGSWENAISDNTKGVDYTPDVRYNPPTPNEGEDR
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7868586; 12948626 [H]