Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 213691577

GI number: 213691577

Start: 784115

End: 785551

Strand: Reverse

Name: purB [H]

Synonym: Blon_0681

Alternate gene names: 213691577

Gene position: 785551-784115 (Counterclockwise)

Preceding gene: 213691578

Following gene: 213691576

Centisome position: 27.73

GC content: 62.49

Gene sequence:

>1437_bases
ATGAAGCTTACCGATATTTCCCCCGCAATCGCATTGACCCCGCTCGACGGCCGTTACCACAAGGCCACCGCTCCGCTCGT
CGAATACATGAGCGAACCTGCCCTGAACCGCGAACGCATGCGTGTCGAAGTCGAATGGATGATTCTTCTGGCCAACGGCT
TTGAGGGCAACGGCAACCAGCCGATCGTTGACGGCGTCAAGCCGTTCACTGCCGAAGAGCAGGCCTTCCTGCGCGCCATT
CCCGAGGACTTCGGCGCCGAAGGCATCAAGCAGCACGCCGCTCATGAGGCCATCACCCACCACGATGTGAAGGCCGTGGA
GTACTACATCGACGATCAGATCGACAAGGCTCCGGCCGAGCTGGCCAACATCAACGACGAGCTGAAGACCCTCGTGCACT
TCGCCTGCACGTCCGAAGACATCAACAACCTCTCCATCGCCCGTTGCGTCAAGAACGCGATGGAAAACGTGTGGACCCCG
GAGTTCAAGCGCATCATCGACCACCTCGCCGGCAAGGCCGAGGAATTCAAGGATATGCCGCTGCTCTCCCTCACCCACGG
CCAGCCGGCCACGCCGACCACGCTCGGCAAGGAGCTCGCCGTCTACGTCTACCGTCTGAACCGTCAGCTCAAGCACATCG
AAAACCAGGAGTACTTGGGCAAGATCAACGGCGCGACCGGCACGTTCGGCGCCCACCTGGCCGCATGCCCGGATGTCGAC
TGGGTCGCCGTCTCCCGCGAATTCGTCGCCAACCGCATGGGCCTGACCTGGAACCCGCTGACCACGCAGATCGAAAGCCA
CGACTGGCAGGCCGAGCTGTACTCCACCGTCTCCCACGCCAACCGCATCATGCACAACCTGTGCGTGGACGTGTGGATGT
ACATCTCCCGCGGCGTGTTCGCCCAGGTGCCGGTCAAGGGTGCCACCGGCTCCTCCACCATGCCGCACAAGGTCAACCCG
ATTCGTTTCGAGAACGCCGAAGCCAACTTCGAGATCAGCTGCTCGCTGCTCGACACCCTCTCCGCCACGCTGGTGGAATC
CCGTTGGCAGCGCGATCTGACCGATTCGACCACCCAGCGCAACATCGGTTCCGCGCTCGGCTACTCCTTGCTCGCCCTGA
CCAACCTCATGGGCGGCCTTGAGTCCATCCACCCGAACACCCACGTCATCGAGCGTGAGCTGGATGAGAACTGGGAGGTG
CTTGGCGAGCCGATCCAGACCGCCATGCGCGCCTGCGAGCTCAAGGGCCTGCCCGGCATGGACAAACCGTACGAGAAGGT
CAAGGAGCTTATGCGCGGCCACTCCATCGACAAGGAGCAGGTCGAGCAGTTCATCGACCAGCAGTCCTTCGACCCGGAAA
CCGCCGCTCGCTTGAAGGCTCTGACCCCCGCCACCTACACCGGTGTGGCCAGCCAGCTCGTCGCTTTCGACCGTTGA

Upstream 100 bases:

>100_bases
GCCAGCATCAGATACCAGGCCATGACCTGTACGGCTCGGGCGGGAACGCCGCACCACAGTATAGCCGCTCGGTTCACGGC
TGGAAGTACCCTTGGAGGTT

Downstream 100 bases:

>100_bases
GATAGGTGCGATGCGAGTCCCTCGAATCCCTCGCATCCACCACACTACTGCGCTGAGTCACAAACCGAACCGCAAACTGA
ACCGAACAAGGACAGACGTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKLTDISPAIALTPLDGRYHKATAPLVEYMSEPALNRERMRVEVEWMILLANGFEGNGNQPIVDGVKPFTAEEQAFLRAI
PEDFGAEGIKQHAAHEAITHHDVKAVEYYIDDQIDKAPAELANINDELKTLVHFACTSEDINNLSIARCVKNAMENVWTP
EFKRIIDHLAGKAEEFKDMPLLSLTHGQPATPTTLGKELAVYVYRLNRQLKHIENQEYLGKINGATGTFGAHLAACPDVD
WVAVSREFVANRMGLTWNPLTTQIESHDWQAELYSTVSHANRIMHNLCVDVWMYISRGVFAQVPVKGATGSSTMPHKVNP
IRFENAEANFEISCSLLDTLSATLVESRWQRDLTDSTTQRNIGSALGYSLLALTNLMGGLESIHPNTHVIERELDENWEV
LGEPIQTAMRACELKGLPGMDKPYEKVKELMRGHSIDKEQVEQFIDQQSFDPETAARLKALTPATYTGVASQLVAFDR

Sequences:

>Translated_478_residues
MKLTDISPAIALTPLDGRYHKATAPLVEYMSEPALNRERMRVEVEWMILLANGFEGNGNQPIVDGVKPFTAEEQAFLRAI
PEDFGAEGIKQHAAHEAITHHDVKAVEYYIDDQIDKAPAELANINDELKTLVHFACTSEDINNLSIARCVKNAMENVWTP
EFKRIIDHLAGKAEEFKDMPLLSLTHGQPATPTTLGKELAVYVYRLNRQLKHIENQEYLGKINGATGTFGAHLAACPDVD
WVAVSREFVANRMGLTWNPLTTQIESHDWQAELYSTVSHANRIMHNLCVDVWMYISRGVFAQVPVKGATGSSTMPHKVNP
IRFENAEANFEISCSLLDTLSATLVESRWQRDLTDSTTQRNIGSALGYSLLALTNLMGGLESIHPNTHVIERELDENWEV
LGEPIQTAMRACELKGLPGMDKPYEKVKELMRGHSIDKEQVEQFIDQQSFDPETAARLKALTPATYTGVASQLVAFDR
>Mature_478_residues
MKLTDISPAIALTPLDGRYHKATAPLVEYMSEPALNRERMRVEVEWMILLANGFEGNGNQPIVDGVKPFTAEEQAFLRAI
PEDFGAEGIKQHAAHEAITHHDVKAVEYYIDDQIDKAPAELANINDELKTLVHFACTSEDINNLSIARCVKNAMENVWTP
EFKRIIDHLAGKAEEFKDMPLLSLTHGQPATPTTLGKELAVYVYRLNRQLKHIENQEYLGKINGATGTFGAHLAACPDVD
WVAVSREFVANRMGLTWNPLTTQIESHDWQAELYSTVSHANRIMHNLCVDVWMYISRGVFAQVPVKGATGSSTMPHKVNP
IRFENAEANFEISCSLLDTLSATLVESRWQRDLTDSTTQRNIGSALGYSLLALTNLMGGLESIHPNTHVIERELDENWEV
LGEPIQTAMRACELKGLPGMDKPYEKVKELMRGHSIDKEQVEQFIDQQSFDPETAARLKALTPATYTGVASQLVAFDR

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=369, Percent_Identity=24.9322493224932, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI183227688, Length=328, Percent_Identity=25.609756097561, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1787376, Length=470, Percent_Identity=45.531914893617, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323391, Length=407, Percent_Identity=26.044226044226, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24647570, Length=401, Percent_Identity=26.1845386533666, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 53407; Mature: 53407

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTDISPAIALTPLDGRYHKATAPLVEYMSEPALNRERMRVEVEWMILLANGFEGNGNQ
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEEEEEEEEECCCCCCCC
PIVDGVKPFTAEEQAFLRAIPEDFGAEGIKQHAAHEAITHHDVKAVEYYIDDQIDKAPAE
CCCCCCCCCCCHHHHHHHHCCHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHH
LANINDELKTLVHFACTSEDINNLSIARCVKNAMENVWTPEFKRIIDHLAGKAEEFKDMP
HHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCC
LLSLTHGQPATPTTLGKELAVYVYRLNRQLKHIENQEYLGKINGATGTFGAHLAACPDVD
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHCCCCCC
WVAVSREFVANRMGLTWNPLTTQIESHDWQAELYSTVSHANRIMHNLCVDVWMYISRGVF
HHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AQVPVKGATGSSTMPHKVNPIRFENAEANFEISCSLLDTLSATLVESRWQRDLTDSTTQR
EECCCCCCCCCCCCCCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHH
NIGSALGYSLLALTNLMGGLESIHPNTHVIERELDENWEVLGEPIQTAMRACELKGLPGM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
DKPYEKVKELMRGHSIDKEQVEQFIDQQSFDPETAARLKALTPATYTGVASQLVAFDR
CCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLTDISPAIALTPLDGRYHKATAPLVEYMSEPALNRERMRVEVEWMILLANGFEGNGNQ
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEEEEEEEEECCCCCCCC
PIVDGVKPFTAEEQAFLRAIPEDFGAEGIKQHAAHEAITHHDVKAVEYYIDDQIDKAPAE
CCCCCCCCCCCHHHHHHHHCCHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHH
LANINDELKTLVHFACTSEDINNLSIARCVKNAMENVWTPEFKRIIDHLAGKAEEFKDMP
HHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCC
LLSLTHGQPATPTTLGKELAVYVYRLNRQLKHIENQEYLGKINGATGTFGAHLAACPDVD
CEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHCCCCCC
WVAVSREFVANRMGLTWNPLTTQIESHDWQAELYSTVSHANRIMHNLCVDVWMYISRGVF
HHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AQVPVKGATGSSTMPHKVNPIRFENAEANFEISCSLLDTLSATLVESRWQRDLTDSTTQR
EECCCCCCCCCCCCCCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHH
NIGSALGYSLLALTNLMGGLESIHPNTHVIERELDENWEVLGEPIQTAMRACELKGLPGM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
DKPYEKVKELMRGHSIDKEQVEQFIDQQSFDPETAARLKALTPATYTGVASQLVAFDR
CCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]