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The map label for this gene is vsr [H]

Identifier: 213691502

GI number: 213691502

Start: 682569

End: 683084

Strand: Direct

Name: vsr [H]

Synonym: Blon_0600

Alternate gene names: 213691502

Gene position: 682569-683084 (Clockwise)

Preceding gene: 213691499

Following gene: 213691504

Centisome position: 24.1

GC content: 54.07

Gene sequence:

>516_bases
ATGGGTGATCGTTCGCTCAGGCTTTCATCCTCGCCGCAACGTCACGGTAGTCTGGAGTGCGTGAATCGCAAGAAACAGCC
AGTGGAGAAGTATGAGCGGGGCACCCGTTCGTACACGATGTCGCATATTCGCGGCAAAGACACCAAAATCGAGGTGCTGG
TACGCAGCTACCTGTTTCGCAAGGGTTTGCGATTCCGCAAGAACGACAAGCGTTACCCCGGCCACCCGGATGTAGTACTG
CCGAAATACCACACCATCGTGTTTGTCAACGGCTGTTTCTGGCACATGCATGAAGGATGTTCCAAACATTCGATGCCGAA
ATCGAACGTGGAATTCTGGCAGGCGAAACTACTGCGCAATCACAATCGAGACATCGCGCAGCGAGCCGAACTGGAGGCCG
CTGGCTGGCGTGTAATTACCGTATGGGAGTGCGAATTAACGAAAGCCGTGCGCGACGAACGCCTCGCACGGCTGTATGAG
CAGATTGTTGAAGGAGATGCAGATTCAGCGGAATAA

Upstream 100 bases:

>100_bases
GGCCCTACGAATCATCATGTCATCCATTTGATTCAGTCTGATCGTGCAGTCTCGACCGGCAGATGATCATTGCCCGCGAT
AAGAGACACGGGCGGGCAAG

Downstream 100 bases:

>100_bases
TTTCAGCTCCCCTCTGGAGGGGAGCTGAAATCAGGATTCAAACGGGAACTTCAGGCCCCAGCGGTCACGAATCGCATCCA
TCAGCTCCATGATGCGGATG

Product: DNA mismatch endonuclease vsr

Products: DNA [C]

Alternate protein names: NA

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MGDRSLRLSSSPQRHGSLECVNRKKQPVEKYERGTRSYTMSHIRGKDTKIEVLVRSYLFRKGLRFRKNDKRYPGHPDVVL
PKYHTIVFVNGCFWHMHEGCSKHSMPKSNVEFWQAKLLRNHNRDIAQRAELEAAGWRVITVWECELTKAVRDERLARLYE
QIVEGDADSAE

Sequences:

>Translated_171_residues
MGDRSLRLSSSPQRHGSLECVNRKKQPVEKYERGTRSYTMSHIRGKDTKIEVLVRSYLFRKGLRFRKNDKRYPGHPDVVL
PKYHTIVFVNGCFWHMHEGCSKHSMPKSNVEFWQAKLLRNHNRDIAQRAELEAAGWRVITVWECELTKAVRDERLARLYE
QIVEGDADSAE
>Mature_170_residues
GDRSLRLSSSPQRHGSLECVNRKKQPVEKYERGTRSYTMSHIRGKDTKIEVLVRSYLFRKGLRFRKNDKRYPGHPDVVLP
KYHTIVFVNGCFWHMHEGCSKHSMPKSNVEFWQAKLLRNHNRDIAQRAELEAAGWRVITVWECELTKAVRDERLARLYEQ
IVEGDADSAE

Specific function: May nick specific sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=143, Percent_Identity=38.4615384615385, Blast_Score=105, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 20083; Mature: 19952

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDRSLRLSSSPQRHGSLECVNRKKQPVEKYERGTRSYTMSHIRGKDTKIEVLVRSYLFR
CCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
KGLRFRKNDKRYPGHPDVVLPKYHTIVFVNGCFWHMHEGCSKHSMPKSNVEFWQAKLLRN
HCCCCCCCCCCCCCCCCEECCCCEEEEEECCHHHHHHHCCHHCCCCCCHHHHHHHHHHHC
HNRDIAQRAELEAAGWRVITVWECELTKAVRDERLARLYEQIVEGDADSAE
CCHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GDRSLRLSSSPQRHGSLECVNRKKQPVEKYERGTRSYTMSHIRGKDTKIEVLVRSYLFR
CCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
KGLRFRKNDKRYPGHPDVVLPKYHTIVFVNGCFWHMHEGCSKHSMPKSNVEFWQAKLLRN
HCCCCCCCCCCCCCCCCEECCCCEEEEEECCHHHHHHHCCHHCCCCCCHHHHHHHHHHHC
HNRDIAQRAELEAAGWRVITVWECELTKAVRDERLARLYEQIVEGDADSAE
CCHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]