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The map label for this gene is folE [H]

Identifier: 213691455

GI number: 213691455

Start: 621938

End: 622519

Strand: Direct

Name: folE [H]

Synonym: Blon_0550

Alternate gene names: 213691455

Gene position: 621938-622519 (Clockwise)

Preceding gene: 213691454

Following gene: 213691456

Centisome position: 21.96

GC content: 62.89

Gene sequence:

>582_bases
ATGAATGAAAACGGGAAGTCCTCATACGACGAGGAAGGCGTGCGCGAAGCCGTACGCCTGTTCCTGAAGTCGATTGGCGA
GGATCCCGAGCGCGAGGGACTGGTGGAAACGCCTGACCGTATCGCCCGCGCGTGCCGCGAGCTGTTCGCCGGTCTGCAGG
CTTCCCCGGCCGATGTGCTGGAGAAGCACTTCGACGTGGATACCGACGAGCTGGTGCTGGTCAAGGACATCGAACTGTAT
TCGGTGTGCGAGCATCACCTGCTGCCGTTCCATGGCGTGGCCCATGTGGGCTATATTCCCGCCAAGGACGGGGTGATGGG
GCTGAGCAAGCTCGCCCGACTGGTCGAGGTGTATGCTCGCCGGCCGCAGGTGCAGGAGCGTTTGACCCAGCAGATCGCCG
ACGCGCTGGTCGAGTACGCGGGCGCACGTGGTGTGATTGTGGTGACCGAATGCGAGCACCTGTGCATGTCGATGCGCGGC
ATCAAGAAATCCTCCGCGCGCACGGTGACCTCTGCGGTGCGCGGCATGCTGCGCAATCCGGCCACCCGAGCCGAAGCCAT
GAGCTTGATTCTTGACAAGTGA

Upstream 100 bases:

>100_bases
GATTCGGCCCGACCTGTCCATGTGGTTTCTCACGTACGCAGGCCGGGCCGACGTCGTTGTGGACAATGACATGGTGAACG
AAACGATTGGGAGGGATCAG

Downstream 100 bases:

>100_bases
CGCAACCTAGGCTCCCCTCTCTGAGGGGAGCGAAGGGAAACAACGAAGGGGAAATAACCTATGACTGATTTGCAGGCGTT
GCATGACAGTACGCGCACCA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MNENGKSSYDEEGVREAVRLFLKSIGEDPEREGLVETPDRIARACRELFAGLQASPADVLEKHFDVDTDELVLVKDIELY
SVCEHHLLPFHGVAHVGYIPAKDGVMGLSKLARLVEVYARRPQVQERLTQQIADALVEYAGARGVIVVTECEHLCMSMRG
IKKSSARTVTSAVRGMLRNPATRAEAMSLILDK

Sequences:

>Translated_193_residues
MNENGKSSYDEEGVREAVRLFLKSIGEDPEREGLVETPDRIARACRELFAGLQASPADVLEKHFDVDTDELVLVKDIELY
SVCEHHLLPFHGVAHVGYIPAKDGVMGLSKLARLVEVYARRPQVQERLTQQIADALVEYAGARGVIVVTECEHLCMSMRG
IKKSSARTVTSAVRGMLRNPATRAEAMSLILDK
>Mature_193_residues
MNENGKSSYDEEGVREAVRLFLKSIGEDPEREGLVETPDRIARACRELFAGLQASPADVLEKHFDVDTDELVLVKDIELY
SVCEHHLLPFHGVAHVGYIPAKDGVMGLSKLARLVEVYARRPQVQERLTQQIADALVEYAGARGVIVVTECEHLCMSMRG
IKKSSARTVTSAVRGMLRNPATRAEAMSLILDK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=172, Percent_Identity=52.9069767441861, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI4503949, Length=172, Percent_Identity=52.9069767441861, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI66932970, Length=142, Percent_Identity=51.4084507042254, Blast_Score=140, Evalue=8e-34,
Organism=Homo sapiens, GI66932972, Length=132, Percent_Identity=54.5454545454545, Blast_Score=140, Evalue=9e-34,
Organism=Escherichia coli, GI1788476, Length=171, Percent_Identity=40.9356725146199, Blast_Score=112, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=176, Percent_Identity=50.5681818181818, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321706, Length=176, Percent_Identity=51.7045454545455, Blast_Score=173, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24656782, Length=179, Percent_Identity=49.1620111731844, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24656796, Length=179, Percent_Identity=49.1620111731844, Blast_Score=166, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24656791, Length=179, Percent_Identity=49.1620111731844, Blast_Score=166, Evalue=8e-42,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 21389; Mature: 21389

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENGKSSYDEEGVREAVRLFLKSIGEDPEREGLVETPDRIARACRELFAGLQASPADVL
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHH
EKHFDVDTDELVLVKDIELYSVCEHHLLPFHGVAHVGYIPAKDGVMGLSKLARLVEVYAR
HHHCCCCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
RPQVQERLTQQIADALVEYAGARGVIVVTECEHLCMSMRGIKKSSARTVTSAVRGMLRNP
CCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
ATRAEAMSLILDK
HHHHHHHHHHHCC
>Mature Secondary Structure
MNENGKSSYDEEGVREAVRLFLKSIGEDPEREGLVETPDRIARACRELFAGLQASPADVL
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHH
EKHFDVDTDELVLVKDIELYSVCEHHLLPFHGVAHVGYIPAKDGVMGLSKLARLVEVYAR
HHHCCCCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
RPQVQERLTQQIADALVEYAGARGVIVVTECEHLCMSMRGIKKSSARTVTSAVRGMLRNP
CCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
ATRAEAMSLILDK
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA