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The map label for this gene is rsmD [H]

Identifier: 213691265

GI number: 213691265

Start: 404983

End: 405594

Strand: Direct

Name: rsmD [H]

Synonym: Blon_0349

Alternate gene names: 213691265

Gene position: 404983-405594 (Clockwise)

Preceding gene: 213691264

Following gene: 213691267

Centisome position: 14.3

GC content: 58.33

Gene sequence:

>612_bases
ATGCGTGTAATTTCTGGACGATTCAAGGGTATGGCGCTTGCTACGCCGAAAACGGGTACCCGGCCTACCACGGATCGCAC
CAAAGAGGCGATTTTCTCGCACCTCGACTCATGGGGAGTGCTGGATGACGCCCGTGTACTGGACCTGTTCGCCGGTACTG
GTGCGTTGGGCATCGAAGCACTGAGCCGTGGCGCGCGAGAACTCGTGGCCGTGGAAAGTTCCCGGCCTGCGGCGGCGCTG
ATTACCAAGACCTTGTCGCAGTTACAGAAGAACCGTTCGTGGGATGCTTCGCTCAAGGCCCGTGTACTGGTCAAAAAAAC
CGAGCAAGTGGCTGGCGGCTTTGGTGAATCCTTTGACGTGATATTCATCGATCCGCCCTACGCCTATGAGACCGCTGAAT
GCAATCAGTTGTTGGCCAATCTGGCGGTGGGCAGCGCCACCAACGAGAACACCGTGATTATGCTCGAACGCTCGGTACGC
TCCGACACCCCCACCGCCCCCGAAGGCTGGCAAATCACTGAAAGCCGCAACTACGGCGAAACCGCCGTCTTCTACATCGA
ATCCGCGGATGACGACTCGGATATAGAGCGTTCCGACGATTCCGCCGCGTAG

Upstream 100 bases:

>100_bases
CCTCATCGCCATCTACCTGAAAACACAGCCAAAAACTCAAAGTGTAAATACACCCTAATTCATGATTGGTATGCGTAGAT
GAGCCTGTACAGTGAAAGCT

Downstream 100 bases:

>100_bases
TGAATCTCCCACCGTGTGGTGAATTAATCGGCGAGCCGGCGCGGATGGGCGGCGGAGACCTCCCAGCCCAAGGCCATCAG
GGTCTTGCGGTCGGCCTTAT

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MRVISGRFKGMALATPKTGTRPTTDRTKEAIFSHLDSWGVLDDARVLDLFAGTGALGIEALSRGARELVAVESSRPAAAL
ITKTLSQLQKNRSWDASLKARVLVKKTEQVAGGFGESFDVIFIDPPYAYETAECNQLLANLAVGSATNENTVIMLERSVR
SDTPTAPEGWQITESRNYGETAVFYIESADDDSDIERSDDSAA

Sequences:

>Translated_203_residues
MRVISGRFKGMALATPKTGTRPTTDRTKEAIFSHLDSWGVLDDARVLDLFAGTGALGIEALSRGARELVAVESSRPAAAL
ITKTLSQLQKNRSWDASLKARVLVKKTEQVAGGFGESFDVIFIDPPYAYETAECNQLLANLAVGSATNENTVIMLERSVR
SDTPTAPEGWQITESRNYGETAVFYIESADDDSDIERSDDSAA
>Mature_203_residues
MRVISGRFKGMALATPKTGTRPTTDRTKEAIFSHLDSWGVLDDARVLDLFAGTGALGIEALSRGARELVAVESSRPAAAL
ITKTLSQLQKNRSWDASLKARVLVKKTEQVAGGFGESFDVIFIDPPYAYETAECNQLLANLAVGSATNENTVIMLERSVR
SDTPTAPEGWQITESRNYGETAVFYIESADDDSDIERSDDSAA

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=196, Percent_Identity=31.6326530612245, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 21980; Mature: 21980

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVISGRFKGMALATPKTGTRPTTDRTKEAIFSHLDSWGVLDDARVLDLFAGTGALGIEA
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHH
LSRGARELVAVESSRPAAALITKTLSQLQKNRSWDASLKARVLVKKTEQVAGGFGESFDV
HHHCHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
IFIDPPYAYETAECNQLLANLAVGSATNENTVIMLERSVRSDTPTAPEGWQITESRNYGE
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCCCC
TAVFYIESADDDSDIERSDDSAA
EEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVISGRFKGMALATPKTGTRPTTDRTKEAIFSHLDSWGVLDDARVLDLFAGTGALGIEA
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHH
LSRGARELVAVESSRPAAALITKTLSQLQKNRSWDASLKARVLVKKTEQVAGGFGESFDV
HHHCHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
IFIDPPYAYETAECNQLLANLAVGSATNENTVIMLERSVRSDTPTAPEGWQITESRNYGE
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCCCC
TAVFYIESADDDSDIERSDDSAA
EEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]