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The map label for this gene is nth [H]

Identifier: 213691195

GI number: 213691195

Start: 330967

End: 331653

Strand: Reverse

Name: nth [H]

Synonym: Blon_0277

Alternate gene names: 213691195

Gene position: 331653-330967 (Counterclockwise)

Preceding gene: 213691196

Following gene: 213691194

Centisome position: 11.71

GC content: 61.28

Gene sequence:

>687_bases
ATGAGAGAAACCAAATCCGCGCGTCTCACGCGCATGCATGACGAATACGACATTCTGCGCCAAGTGATTCCGGCACCAGC
TTGTGCACTGCATTTCTCGAATCCGCTCGAATTGCTGATCGCCACCGTGCTCAGCGCCCAGACTACCGACAAGCGCGTCA
ACACGGTCACCCCCGAACTCTTCGCCACCTTCCCCACCGCTCGTGACTTGGCGGCCGCGAATCCGGCCCAAGTCGAAGAC
ATCATTCATCCACTGGGGTTCTATCGTTCGAAAACCCAGCATCTCATCGGACTGGCCACGGCACTGGACGAACGGTTCGG
CGGCGTGGTGCCGCGCACGATGGATGGACTGACCAGCTTGCCGGGGGTCGGCCGCAAAACCGCGAACGTCGTGCTCGGCA
ACGCATTCGATATCCCCGGATTCCCGGTCGATACGCATGTGATGCGCGTAACCGGACGACTGCGTTGGCGGTCGGATTGG
CGGTCCGCGCACCCTGATCCCGTGAAAATCGAGAAGGAAATCACCGCCTGTTTTCCGCCCGAAGAGTGGACGAATCTCAG
CCACCGACTGATTCTGTTCGGCCGGGCCACATGCCATGCACGTACCCCTGACTGCGCCAACTGCCCGCTGTCCGACACCT
GCCCTAGCTATGCGCCGCCCGCCGGCAAGAGCACGTCCAAGCACTAG

Upstream 100 bases:

>100_bases
GACATCGATGACGATACTCCCGATCTCAACACCGCCGCCTGACAGACCAGCAGCGCTGCAAACTATCAATCATCGACACT
ACGCCGCTTAGATCCATACG

Downstream 100 bases:

>100_bases
CTCATGCCCATCATGGTGAATTGCGGATTCTAGGCTGAGTCCACCGCTAGACTGAAGACCATGCAGCACAGCAAGCGGAA
CAATACATGGTCCAAGTGGG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MRETKSARLTRMHDEYDILRQVIPAPACALHFSNPLELLIATVLSAQTTDKRVNTVTPELFATFPTARDLAAANPAQVED
IIHPLGFYRSKTQHLIGLATALDERFGGVVPRTMDGLTSLPGVGRKTANVVLGNAFDIPGFPVDTHVMRVTGRLRWRSDW
RSAHPDPVKIEKEITACFPPEEWTNLSHRLILFGRATCHARTPDCANCPLSDTCPSYAPPAGKSTSKH

Sequences:

>Translated_228_residues
MRETKSARLTRMHDEYDILRQVIPAPACALHFSNPLELLIATVLSAQTTDKRVNTVTPELFATFPTARDLAAANPAQVED
IIHPLGFYRSKTQHLIGLATALDERFGGVVPRTMDGLTSLPGVGRKTANVVLGNAFDIPGFPVDTHVMRVTGRLRWRSDW
RSAHPDPVKIEKEITACFPPEEWTNLSHRLILFGRATCHARTPDCANCPLSDTCPSYAPPAGKSTSKH
>Mature_228_residues
MRETKSARLTRMHDEYDILRQVIPAPACALHFSNPLELLIATVLSAQTTDKRVNTVTPELFATFPTARDLAAANPAQVED
IIHPLGFYRSKTQHLIGLATALDERFGGVVPRTMDGLTSLPGVGRKTANVVLGNAFDIPGFPVDTHVMRVTGRLRWRSDW
RSAHPDPVKIEKEITACFPPEEWTNLSHRLILFGRATCHARTPDCANCPLSDTCPSYAPPAGKSTSKH

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=181, Percent_Identity=30.939226519337, Blast_Score=101, Evalue=5e-22,
Organism=Escherichia coli, GI1787920, Length=196, Percent_Identity=39.7959183673469, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1789331, Length=213, Percent_Identity=28.169014084507, Blast_Score=68, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=194, Percent_Identity=32.9896907216495, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=209, Percent_Identity=27.7511961722488, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6319304, Length=177, Percent_Identity=28.2485875706215, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=29.0502793296089, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25131; Mature: 25131

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRETKSARLTRMHDEYDILRQVIPAPACALHFSNPLELLIATVLSAQTTDKRVNTVTPEL
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHCCHHH
FATFPTARDLAAANPAQVEDIIHPLGFYRSKTQHLIGLATALDERFGGVVPRTMDGLTSL
HHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
PGVGRKTANVVLGNAFDIPGFPVDTHVMRVTGRLRWRSDWRSAHPDPVKIEKEITACFPP
CCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHEECCHHCCCCCCCEEEHHHHHCCCCC
EEWTNLSHRLILFGRATCHARTPDCANCPLSDTCPSYAPPAGKSTSKH
HHHCCCCCEEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRETKSARLTRMHDEYDILRQVIPAPACALHFSNPLELLIATVLSAQTTDKRVNTVTPEL
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHCCHHH
FATFPTARDLAAANPAQVEDIIHPLGFYRSKTQHLIGLATALDERFGGVVPRTMDGLTSL
HHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
PGVGRKTANVVLGNAFDIPGFPVDTHVMRVTGRLRWRSDWRSAHPDPVKIEKEITACFPP
CCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHEECCHHCCCCCCCEEEHHHHHCCCCC
EEWTNLSHRLILFGRATCHARTPDCANCPLSDTCPSYAPPAGKSTSKH
HHHCCCCCEEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]