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The map label for this gene is dagK [H]

Identifier: 213691079

GI number: 213691079

Start: 185399

End: 186529

Strand: Direct

Name: dagK [H]

Synonym: Blon_0158

Alternate gene names: 213691079

Gene position: 185399-186529 (Clockwise)

Preceding gene: 213691078

Following gene: 213691081

Centisome position: 6.54

GC content: 61.8

Gene sequence:

>1131_bases
ATGAGCGAGAGTGAACGCACAGAACGCCGTCGCGTGGTGGCCATGGTGGGCAATCCCACTTCCGATAAGGGTCGTGGTGC
CAAGGTGGATGCGCGAGTATTGGGACTGCTGGAGGACGCCGGCCGTGCCCATAACTTCGACGTCATCGACATCACCGGCA
CGAGTTTCGACGATTCCCTGAACCAGGCGCGTGAACGCGCGCACGAGTACGACTATCTGGTGGTGGTCGGCGGCGACGGC
ATGATCGCACTCGGCGCCAATGCCGTGGGTTGCGGCGGTAAGCCGTTGGGCATCGTGGCCATGGGCTCGGGCAATGATTT
CGCTCGGGGACTTAAGCTGCCGGTCAATCGCATCGAAACCGCAGTGGAAGGTATCGTCGGCGCTATCGTGCGCGGATCAC
ACATTGATGTGGACATGGGACGCGTGACCTCTTTAGACGGTGGCTATGCCGTGAACCCATCCACCGGCGAGGAATACGAG
TACCAGGAGAGCAAGTCTGCCGAGCATCCGATTGACCGGTACTATGCGGGCATGCTGTCCTGTGGTCTCGACGCGAGCAT
CAACGATCGTGCGAATCATTCGCATCTGCCGACCGGCACCATGCGCTACTTCGTGGCGGTGCTGGTGGAGCTGACGCATA
TGAAGCGTTATGGGTACCACATCAAAGCCACGCTGGCGGACGGTACCACGGATGAGCGGGACATCATCACGCCGTTGCTG
ACGATTGCGAATTCGCGTCATATCGGTGGCGGCATCGAGGTCTCGCCGTACTCCTGCTTCTCCGACGGGCTGTTGGACCT
GGTGTGGATGAATCACGTGCCGAACTTCGGCGAATGTGCGGTGGCCATCTCGAACGCCTATAATGGCAAGCTGCTCGCCT
CAAAGGTGTTCGGGTGGAAGCGCATACGCGAGATCGAAGTCACGCGGGCAGCGGAAGGAGACGAGCCACCGGTGCTGATG
GCGGACGGCGAATACATCGGGCATCTGCCCTTCAGGGTGGTGGCCGAGGACTGCGCACTGCGCGTGCTGGTGCCGCCGGC
CGTAGCCGCCCGTGAGGTGGACTCCCGACAGGAAGTGCTTGACGCCATCGCCCGAGACGGTCGCGACCCCGTAACCGGCC
AGTTCGCCTGA

Upstream 100 bases:

>100_bases
CCGGACCGGTGAATTGTACGGAAGGAGACAATTATCACAATAATGGACCGTAAGCACTGATGCTCTGGCGGGCTTCTGGA
CCTTTGGTTAAGGTGAGAGC

Downstream 100 bases:

>100_bases
CCTGATTATATTGGCTCCCCTCATTAGGCTGAGCCGTAAAGCAAACAGCCCAGTGCTGTTTATAGGCGAGGGTTCGACGA
TAGTCGAACCAGAAGAGAGT

Product: diacylglycerol kinase, catalytic region

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSESERTERRRVVAMVGNPTSDKGRGAKVDARVLGLLEDAGRAHNFDVIDITGTSFDDSLNQARERAHEYDYLVVVGGDG
MIALGANAVGCGGKPLGIVAMGSGNDFARGLKLPVNRIETAVEGIVGAIVRGSHIDVDMGRVTSLDGGYAVNPSTGEEYE
YQESKSAEHPIDRYYAGMLSCGLDASINDRANHSHLPTGTMRYFVAVLVELTHMKRYGYHIKATLADGTTDERDIITPLL
TIANSRHIGGGIEVSPYSCFSDGLLDLVWMNHVPNFGECAVAISNAYNGKLLASKVFGWKRIREIEVTRAAEGDEPPVLM
ADGEYIGHLPFRVVAEDCALRVLVPPAVAAREVDSRQEVLDAIARDGRDPVTGQFA

Sequences:

>Translated_376_residues
MSESERTERRRVVAMVGNPTSDKGRGAKVDARVLGLLEDAGRAHNFDVIDITGTSFDDSLNQARERAHEYDYLVVVGGDG
MIALGANAVGCGGKPLGIVAMGSGNDFARGLKLPVNRIETAVEGIVGAIVRGSHIDVDMGRVTSLDGGYAVNPSTGEEYE
YQESKSAEHPIDRYYAGMLSCGLDASINDRANHSHLPTGTMRYFVAVLVELTHMKRYGYHIKATLADGTTDERDIITPLL
TIANSRHIGGGIEVSPYSCFSDGLLDLVWMNHVPNFGECAVAISNAYNGKLLASKVFGWKRIREIEVTRAAEGDEPPVLM
ADGEYIGHLPFRVVAEDCALRVLVPPAVAAREVDSRQEVLDAIARDGRDPVTGQFA
>Mature_375_residues
SESERTERRRVVAMVGNPTSDKGRGAKVDARVLGLLEDAGRAHNFDVIDITGTSFDDSLNQARERAHEYDYLVVVGGDGM
IALGANAVGCGGKPLGIVAMGSGNDFARGLKLPVNRIETAVEGIVGAIVRGSHIDVDMGRVTSLDGGYAVNPSTGEEYEY
QESKSAEHPIDRYYAGMLSCGLDASINDRANHSHLPTGTMRYFVAVLVELTHMKRYGYHIKATLADGTTDERDIITPLLT
IANSRHIGGGIEVSPYSCFSDGLLDLVWMNHVPNFGECAVAISNAYNGKLLASKVFGWKRIREIEVTRAAEGDEPPVLMA
DGEYIGHLPFRVVAEDCALRVLVPPAVAAREVDSRQEVLDAIARDGRDPVTGQFA

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derive

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Secreted, cell wall (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 40546; Mature: 40415

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESERTERRRVVAMVGNPTSDKGRGAKVDARVLGLLEDAGRAHNFDVIDITGTSFDDSL
CCCCHHHHHEEEEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCHHHH
NQARERAHEYDYLVVVGGDGMIALGANAVGCGGKPLGIVAMGSGNDFARGLKLPVNRIET
HHHHHHHHCCCEEEEECCCCEEEECCCEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
AVEGIVGAIVRGSHIDVDMGRVTSLDGGYAVNPSTGEEYEYQESKSAEHPIDRYYAGMLS
HHHHHHHHHHCCCCCEEECCCEEECCCCEEECCCCCCCCCCHHCCCCCCCHHHHHHHHHH
CGLDASINDRANHSHLPTGTMRYFVAVLVELTHMKRYGYHIKATLADGTTDERDIITPLL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHH
TIANSRHIGGGIEVSPYSCFSDGLLDLVWMNHVPNFGECAVAISNAYNGKLLASKVFGWK
HHCCCCCCCCCCEECCCHHHHCCHHHHHHHHCCCCCCCEEEEEECCCCCEEHHHHHHHHH
RIREIEVTRAAEGDEPPVLMADGEYIGHLPFRVVAEDCALRVLVPPAVAAREVDSRQEVL
HHHEEEEEECCCCCCCCEEEECCCEECCCCHHEECCCCEEEEEECCCHHHHHCCHHHHHH
DAIARDGRDPVTGQFA
HHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SESERTERRRVVAMVGNPTSDKGRGAKVDARVLGLLEDAGRAHNFDVIDITGTSFDDSL
CCCHHHHHEEEEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCCCHHHH
NQARERAHEYDYLVVVGGDGMIALGANAVGCGGKPLGIVAMGSGNDFARGLKLPVNRIET
HHHHHHHHCCCEEEEECCCCEEEECCCEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
AVEGIVGAIVRGSHIDVDMGRVTSLDGGYAVNPSTGEEYEYQESKSAEHPIDRYYAGMLS
HHHHHHHHHHCCCCCEEECCCEEECCCCEEECCCCCCCCCCHHCCCCCCCHHHHHHHHHH
CGLDASINDRANHSHLPTGTMRYFVAVLVELTHMKRYGYHIKATLADGTTDERDIITPLL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHH
TIANSRHIGGGIEVSPYSCFSDGLLDLVWMNHVPNFGECAVAISNAYNGKLLASKVFGWK
HHCCCCCCCCCCEECCCHHHHCCHHHHHHHHCCCCCCCEEEEEECCCCCEEHHHHHHHHH
RIREIEVTRAAEGDEPPVLMADGEYIGHLPFRVVAEDCALRVLVPPAVAAREVDSRQEVL
HHHEEEEEECCCCCCCCEEEECCCEECCCCHHEECCCCEEEEEECCCHHHHHCCHHHHHH
DAIARDGRDPVTGQFA
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]