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The map label for this gene is tgt [H]

Identifier: 213691022

GI number: 213691022

Start: 118013

End: 119326

Strand: Reverse

Name: tgt [H]

Synonym: Blon_0101

Alternate gene names: 213691022

Gene position: 119326-118013 (Counterclockwise)

Preceding gene: 213691040

Following gene: 213691021

Centisome position: 4.21

GC content: 63.93

Gene sequence:

>1314_bases
ATGACGATCAGCCTTCTTGAACATCCGTTGGGCGCGCAGCCCGGCCAGCCCGGCGACCGCAGCGCCTTCCATTTCGAAAC
AATCACCCGCCTGCCATCCACGGCCAGCGGGCTTGGGCGAGACGGTGCCCGATACGGACGCACCGGCATCATCCACACGC
CCCACGGCGATATTCGCACACCCGCGTTCGTGCCGGTGAGCACGCAAGCCGCCATGAAGGCCGTGCTGCCCGAGCAAATG
AAGGAGCTCGGCGCGCAGTGTCTGCTGGCCAACGCCTTCCACCTGTTCGAACGCCCGGGCGAGGCCGTGCTGGACGCGGC
CGGCGGGCTGGCGAACTTCATGAACTGGGACGGGCCCACGTTCACCGATTCCGGCGGATTCCAGGTCATGTCCCTAGGAG
TCGGGTTCAAAAAGACGCTGGCGATGGACGTGACCGGCATGAAGTCGGACGACATCATCGCCAAGGGCAAGGAACGCATG
GCCTGGGTGGACGAGGACGGCGTGACATTCAAGTCGCCTCTGAATGGTGACGCACATCGTTTCTCCGCTGAGATTTCGAT
GGGCATCCAGCACAAAATCGGCGCAGACATCATGTTCGCGTTCGACGAACTCACCACGCTGATGAACACCCGCTCCTACC
AGGAAGACTCGGTGGAGCGCACCTTCCGCTGGGCCCGTCGCTGCGTGGACGAGCACCAGCGGCTGACGGCGGAACGCCTG
GGCAGGCCGTATCAGGCCTTGTACGGCGTGGTGCAGGGCGCGAACTATGAGGACCTGCGCCGGCGCGCGGCCGCGCAGAT
CGCCTCTCTGGACTTCGACGGCGTGGGCATCGGCGGCGCGATCGAGAAGCGTCTCATCGGCGACACCTGCGCATGGATCT
GCGACGCGATGCCGGAGAACCGTCCACGTCATGTGCTCGGCATCGCCTCCGTGGATGACATCTTCGCCTGCGTGGAGAAC
GGCGGCGACACCTTCGACTGCGTGGCCCCCGCACGTTGCGCCCGCAACGGCGCGATCTACACGAGGTCGGGCCGATACAA
CATCAAACGCGCCCAGCACAAGTTCGATTTCGGCCCGCTGGAGGAAGGATGCGACTGCTACACGTGCCGTCACTACTCGC
GCGCATACGTCGATCACCTGCTTCGCGCACGCGAATTCAACGGTTTCACGTTGGCCACGATTCACAACGAACATTTCTTC
GTGAAGCTGCTGGACGACATCCGCGCCGCCATCGACGGCGGATACTTCGATGAGTTCCGCGACGACGCGATGGCTCACTT
CTACGGCAACGGCGAACGCAAGCCGGGACTGTGA

Upstream 100 bases:

>100_bases
AAGCGGAAGTAGACGTTCGGGAGCGGCGAAATCCGCCCCTCGAAACCGAGTTCTTCACAATTTCCGGCCCTTTTCTGGCC
CTTCTGCCTAAAGTGAACAT

Downstream 100 bases:

>100_bases
TGCCGTAGCCGTCGCGCGCCGACGGCCGCGGCATCATGCCGCCACCTGCGGATCGCCGAACGGCAACCGCCAACCGCCAA
CCGCGACACGCCGAAGATTG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTISLLEHPLGAQPGQPGDRSAFHFETITRLPSTASGLGRDGARYGRTGIIHTPHGDIRTPAFVPVSTQAAMKAVLPEQM
KELGAQCLLANAFHLFERPGEAVLDAAGGLANFMNWDGPTFTDSGGFQVMSLGVGFKKTLAMDVTGMKSDDIIAKGKERM
AWVDEDGVTFKSPLNGDAHRFSAEISMGIQHKIGADIMFAFDELTTLMNTRSYQEDSVERTFRWARRCVDEHQRLTAERL
GRPYQALYGVVQGANYEDLRRRAAAQIASLDFDGVGIGGAIEKRLIGDTCAWICDAMPENRPRHVLGIASVDDIFACVEN
GGDTFDCVAPARCARNGAIYTRSGRYNIKRAQHKFDFGPLEEGCDCYTCRHYSRAYVDHLLRAREFNGFTLATIHNEHFF
VKLLDDIRAAIDGGYFDEFRDDAMAHFYGNGERKPGL

Sequences:

>Translated_437_residues
MTISLLEHPLGAQPGQPGDRSAFHFETITRLPSTASGLGRDGARYGRTGIIHTPHGDIRTPAFVPVSTQAAMKAVLPEQM
KELGAQCLLANAFHLFERPGEAVLDAAGGLANFMNWDGPTFTDSGGFQVMSLGVGFKKTLAMDVTGMKSDDIIAKGKERM
AWVDEDGVTFKSPLNGDAHRFSAEISMGIQHKIGADIMFAFDELTTLMNTRSYQEDSVERTFRWARRCVDEHQRLTAERL
GRPYQALYGVVQGANYEDLRRRAAAQIASLDFDGVGIGGAIEKRLIGDTCAWICDAMPENRPRHVLGIASVDDIFACVEN
GGDTFDCVAPARCARNGAIYTRSGRYNIKRAQHKFDFGPLEEGCDCYTCRHYSRAYVDHLLRAREFNGFTLATIHNEHFF
VKLLDDIRAAIDGGYFDEFRDDAMAHFYGNGERKPGL
>Mature_436_residues
TISLLEHPLGAQPGQPGDRSAFHFETITRLPSTASGLGRDGARYGRTGIIHTPHGDIRTPAFVPVSTQAAMKAVLPEQMK
ELGAQCLLANAFHLFERPGEAVLDAAGGLANFMNWDGPTFTDSGGFQVMSLGVGFKKTLAMDVTGMKSDDIIAKGKERMA
WVDEDGVTFKSPLNGDAHRFSAEISMGIQHKIGADIMFAFDELTTLMNTRSYQEDSVERTFRWARRCVDEHQRLTAERLG
RPYQALYGVVQGANYEDLRRRAAAQIASLDFDGVGIGGAIEKRLIGDTCAWICDAMPENRPRHVLGIASVDDIFACVENG
GDTFDCVAPARCARNGAIYTRSGRYNIKRAQHKFDFGPLEEGCDCYTCRHYSRAYVDHLLRAREFNGFTLATIHNEHFFV
KLLDDIRAAIDGGYFDEFRDDAMAHFYGNGERKPGL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=393, Percent_Identity=32.5699745547074, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI1786607, Length=379, Percent_Identity=37.7308707124011, Blast_Score=266, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17544680, Length=391, Percent_Identity=34.2710997442455, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19920510, Length=402, Percent_Identity=34.0796019900498, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 48323; Mature: 48192

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISLLEHPLGAQPGQPGDRSAFHFETITRLPSTASGLGRDGARYGRTGIIHTPHGDIRT
CCCCCCCCCCCCCCCCCCCCCCEEHHHHHHCCCCHHCCCCCCHHHCCCCEEECCCCCCCC
PAFVPVSTQAAMKAVLPEQMKELGAQCLLANAFHLFERPGEAVLDAAGGLANFMNWDGPT
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHCCCCCC
FTDSGGFQVMSLGVGFKKTLAMDVTGMKSDDIIAKGKERMAWVDEDGVTFKSPLNGDAHR
EECCCCCEEEEECCCHHHHHEEEECCCCCCCHHHCCCCCEEEECCCCCEECCCCCCCHHH
FSAEISMGIQHKIGADIMFAFDELTTLMNTRSYQEDSVERTFRWARRCVDEHQRLTAERL
HHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GRPYQALYGVVQGANYEDLRRRAAAQIASLDFDGVGIGGAIEKRLIGDTCAWICDAMPEN
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
RPRHVLGIASVDDIFACVENGGDTFDCVAPARCARNGAIYTRSGRYNIKRAQHKFDFGPL
CCCEEEEECCHHHHHHHHHCCCCEEECCCHHHHHCCCCEEEECCCCCHHHHHHCCCCCCC
EEGCDCYTCRHYSRAYVDHLLRAREFNGFTLATIHNEHFFVKLLDDIRAAIDGGYFDEFR
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHH
DDAMAHFYGNGERKPGL
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TISLLEHPLGAQPGQPGDRSAFHFETITRLPSTASGLGRDGARYGRTGIIHTPHGDIRT
CCCCCCCCCCCCCCCCCCCCCEEHHHHHHCCCCHHCCCCCCHHHCCCCEEECCCCCCCC
PAFVPVSTQAAMKAVLPEQMKELGAQCLLANAFHLFERPGEAVLDAAGGLANFMNWDGPT
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHCCCCCC
FTDSGGFQVMSLGVGFKKTLAMDVTGMKSDDIIAKGKERMAWVDEDGVTFKSPLNGDAHR
EECCCCCEEEEECCCHHHHHEEEECCCCCCCHHHCCCCCEEEECCCCCEECCCCCCCHHH
FSAEISMGIQHKIGADIMFAFDELTTLMNTRSYQEDSVERTFRWARRCVDEHQRLTAERL
HHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GRPYQALYGVVQGANYEDLRRRAAAQIASLDFDGVGIGGAIEKRLIGDTCAWICDAMPEN
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
RPRHVLGIASVDDIFACVENGGDTFDCVAPARCARNGAIYTRSGRYNIKRAQHKFDFGPL
CCCEEEEECCHHHHHHHHHCCCCEEECCCHHHHHCCCCEEEECCCCCHHHHHHCCCCCCC
EEGCDCYTCRHYSRAYVDHLLRAREFNGFTLATIHNEHFFVKLLDDIRAAIDGGYFDEFR
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHH
DDAMAHFYGNGERKPGL
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA