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The map label for this gene is liaR [H]

Identifier: 213690988

GI number: 213690988

Start: 78467

End: 79138

Strand: Direct

Name: liaR [H]

Synonym: Blon_0066

Alternate gene names: 213690988

Gene position: 78467-79138 (Clockwise)

Preceding gene: 213690987

Following gene: 213690989

Centisome position: 2.77

GC content: 61.76

Gene sequence:

>672_bases
ATGTCGGATATCAGCGGGCGGGATGGATCGCAGCCCACCATGCTCATCATGGACAACGACCGCATGGCCTTGATGGCATT
GCAGTCGATTCTGGCGAAGGCACTGCCCGACTTCGAATTGTTGCCGCCGGTGAGCGAGGGCAGCAAAGCCATTCAGCTGT
GCACGGCCACCAAACGCCCGCCTGCTGTGCTATTGGCCGATATCGCGATGAACGACATCAACGGCCCGGATGTGGTGCGT
GCAATCCGCAGGGAAAACGCGACCACGGCGATACTGGCCATTACCGCCTCGATTGTGGGCCGACATGCCAAAGAGATGGC
CCGGGCCGGCGCACAGGGCATCATGAGCAAGAACGATGATGTGCGGTTGCAGGCCGTGGCGATTCGTCAGGTACAGGCCG
GCAAAATATGGGGCGATGGTGGCGACGTGCGGTTCGAGACCGCGGCCGTTGCGTCCGCGCGCATCAAGGCCGAGAAGGAC
AACCGGCTGAGCGCGCGTGAGATGGAGGTTGCGGAACTGTGGTCCCGAGGCCGGACCATGCCGCAGATCGCCACCGAACT
GTCCATCACCGAAACCACCGTGCGTACGCATTTGATGCGTGCGGCCGACAAGCTCGGCGCCGGCAATCTCAAGGAACTTA
TCGGCGCATGGATTCGCATGAACCTGCACTGA

Upstream 100 bases:

>100_bases
CGCCGGCCGCGCCCAAGCCGAAGCCGGCCCCGCGCAAGCCGATTCCGATGCCCATGCCCTCAGCGCTGAAGCCGAAGAAG
AGCTGAACGCACGTAGGCAT

Downstream 100 bases:

>100_bases
TGAAGGCGCCGATGGGTGTGTGGGGACACGGTAGAAGAAGCGCAAGACGCATAAGGGGAGCGGCCCGGTGAAACGTCCGA
TTATCATTTCGCGGCATTCG

Product: two component transcriptional regulator, LuxR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSDISGRDGSQPTMLIMDNDRMALMALQSILAKALPDFELLPPVSEGSKAIQLCTATKRPPAVLLADIAMNDINGPDVVR
AIRRENATTAILAITASIVGRHAKEMARAGAQGIMSKNDDVRLQAVAIRQVQAGKIWGDGGDVRFETAAVASARIKAEKD
NRLSAREMEVAELWSRGRTMPQIATELSITETTVRTHLMRAADKLGAGNLKELIGAWIRMNLH

Sequences:

>Translated_223_residues
MSDISGRDGSQPTMLIMDNDRMALMALQSILAKALPDFELLPPVSEGSKAIQLCTATKRPPAVLLADIAMNDINGPDVVR
AIRRENATTAILAITASIVGRHAKEMARAGAQGIMSKNDDVRLQAVAIRQVQAGKIWGDGGDVRFETAAVASARIKAEKD
NRLSAREMEVAELWSRGRTMPQIATELSITETTVRTHLMRAADKLGAGNLKELIGAWIRMNLH
>Mature_222_residues
SDISGRDGSQPTMLIMDNDRMALMALQSILAKALPDFELLPPVSEGSKAIQLCTATKRPPAVLLADIAMNDINGPDVVRA
IRRENATTAILAITASIVGRHAKEMARAGAQGIMSKNDDVRLQAVAIRQVQAGKIWGDGGDVRFETAAVASARIKAEKDN
RLSAREMEVAELWSRGRTMPQIATELSITETTVRTHLMRAADKLGAGNLKELIGAWIRMNLH

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788712, Length=199, Percent_Identity=27.1356783919598, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788521, Length=186, Percent_Identity=28.494623655914, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24135; Mature: 24004

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDISGRDGSQPTMLIMDNDRMALMALQSILAKALPDFELLPPVSEGSKAIQLCTATKRP
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEECCCCCC
PAVLLADIAMNDINGPDVVRAIRRENATTAILAITASIVGRHAKEMARAGAQGIMSKNDD
CCEEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VRLQAVAIRQVQAGKIWGDGGDVRFETAAVASARIKAEKDNRLSAREMEVAELWSRGRTM
CEEEEEEEHHHHCCEEECCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCC
PQIATELSITETTVRTHLMRAADKLGAGNLKELIGAWIRMNLH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDISGRDGSQPTMLIMDNDRMALMALQSILAKALPDFELLPPVSEGSKAIQLCTATKRP
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEECCCCCC
PAVLLADIAMNDINGPDVVRAIRRENATTAILAITASIVGRHAKEMARAGAQGIMSKNDD
CCEEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VRLQAVAIRQVQAGKIWGDGGDVRFETAAVASARIKAEKDNRLSAREMEVAELWSRGRTM
CEEEEEEEHHHHCCEEECCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCC
PQIATELSITETTVRTHLMRAADKLGAGNLKELIGAWIRMNLH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]