Click here to switch to the map view.

The map label for this gene is yfkN [H]

Identifier: 213690945

GI number: 213690945

Start: 19995

End: 21257

Strand: Reverse

Name: yfkN [H]

Synonym: Blon_0019

Alternate gene names: 213690945

Gene position: 21257-19995 (Counterclockwise)

Preceding gene: 213690946

Following gene: 213690944

Centisome position: 0.75

GC content: 63.18

Gene sequence:

>1263_bases
ATGACCACGATCACGATCCTCGCCACCAGCGACCTGCACGGCTTCCTGCCCGACACCCTCGCGGACGTGCCCGGCGACCC
GTCCGCCGCGAACGCCCACGCGGCCGGCATACTCGCCGCACACGCCGCCAAGCGGATGACGGGCGTGGACGACGCCACGC
TGCTCATCGACGCCGGCGACTACCTGCTAGGAAGCGCCTACGCCACCTTCACCGCCCGATCGGCCGAGAGGGAATCCCCG
CTCACCCGCGTCGCCTCCGCCTGCGGCTACACCGCCATGGCCCTGGGCAACCACGACTTCGACCACGGCACCGCACTGCC
CGCAAGCCAGAACCCCCACCTGCACGAACGGCTCCTGTGCTGCAACGTGACGGACGAGGAAGGCCACCCGATCTTCCATC
CATACCGACTCGTCCAAGCCCGGGGCATCCGTGTGGGCATCGTGGGCGCGGTTACCGGAGCCCTGCCCCAACTCACCGCG
TTCCGCAACACACAGCACATCCACGTCCTGGACGCGGTCGAAAGCATCAGGACCACCGTCAACCGGATACGCGCGGACGT
GGACCTGCTCATCGTCGCCTACCACGGCGGCATCGAATGCGACATGGCCAGCGGACGACCCACGCAATACGACACCGGCG
AGGACCAGGCATACCGCATCCTCTCCACCATCCCCGGCATCGACGGGCTGATCTGCGGACACCAACACCGCACCAACCAC
GGCGAATGCCACGGCATCCCCTACGTGCAACCCGGATACCAGGGAAACTCCGTCGGATTCATCTCCTACCAGTTCAAGGA
AAGGCGAATCAGCCGGCACGAGACCGCGATGCTGCACCCGACCACTGACAAGCTGGAACCATTGGATCCGACGACGATGA
ACCTCAAGCTGGACGAATACAGGACCTGGCTGGACCAGCCCATCGACACCGGACGGTTCGGTGAATACATCACGCTGAAA
ACCGGCATCAGACTCCAACGATTCATCTGGCGGAAAACCAGCGACGGAACCACGACCATCCGCGAATTCCACCACAGCTT
CCCCAAACCCTACACCGCATCCGTGTTCCGCCTGACCTGGGAAGAACTCCGGACCTGCATCGACCAAGGCCTGATACAAG
CCGACATGATCCCCGCAACGGAAGCACCGTTGGGCGGATACCAAGTCATCACCAACACACCCGAGGCATTCCCCACATAC
CGACTGGAAAGACGAACAGTGGACAACCTCTTCGACGAATACCTACACTGGCTGAAGCAATAA

Upstream 100 bases:

>100_bases
CAGGGTCAGCATCATCCTGCTGGGCATCATCATCGTGGTCGCGCTCATCGACGCGGCGACCGGATGGCTACGCAAGAAAC
TCCTCTAAAAGGCCTGCCAC

Downstream 100 bases:

>100_bases
GGGTTTGGGGAGATAAGTCGTACTCCGGCTTCTTCTCCCTAAACCTCAGTTTCATATGACGTATGCACGCGACGATCATG
TCGAACAGCGCTATGGGCCA

Product: metallophosphoesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTTITILATSDLHGFLPDTLADVPGDPSAANAHAAGILAAHAAKRMTGVDDATLLIDAGDYLLGSAYATFTARSAERESP
LTRVASACGYTAMALGNHDFDHGTALPASQNPHLHERLLCCNVTDEEGHPIFHPYRLVQARGIRVGIVGAVTGALPQLTA
FRNTQHIHVLDAVESIRTTVNRIRADVDLLIVAYHGGIECDMASGRPTQYDTGEDQAYRILSTIPGIDGLICGHQHRTNH
GECHGIPYVQPGYQGNSVGFISYQFKERRISRHETAMLHPTTDKLEPLDPTTMNLKLDEYRTWLDQPIDTGRFGEYITLK
TGIRLQRFIWRKTSDGTTTIREFHHSFPKPYTASVFRLTWEELRTCIDQGLIQADMIPATEAPLGGYQVITNTPEAFPTY
RLERRTVDNLFDEYLHWLKQ

Sequences:

>Translated_420_residues
MTTITILATSDLHGFLPDTLADVPGDPSAANAHAAGILAAHAAKRMTGVDDATLLIDAGDYLLGSAYATFTARSAERESP
LTRVASACGYTAMALGNHDFDHGTALPASQNPHLHERLLCCNVTDEEGHPIFHPYRLVQARGIRVGIVGAVTGALPQLTA
FRNTQHIHVLDAVESIRTTVNRIRADVDLLIVAYHGGIECDMASGRPTQYDTGEDQAYRILSTIPGIDGLICGHQHRTNH
GECHGIPYVQPGYQGNSVGFISYQFKERRISRHETAMLHPTTDKLEPLDPTTMNLKLDEYRTWLDQPIDTGRFGEYITLK
TGIRLQRFIWRKTSDGTTTIREFHHSFPKPYTASVFRLTWEELRTCIDQGLIQADMIPATEAPLGGYQVITNTPEAFPTY
RLERRTVDNLFDEYLHWLKQ
>Mature_419_residues
TTITILATSDLHGFLPDTLADVPGDPSAANAHAAGILAAHAAKRMTGVDDATLLIDAGDYLLGSAYATFTARSAERESPL
TRVASACGYTAMALGNHDFDHGTALPASQNPHLHERLLCCNVTDEEGHPIFHPYRLVQARGIRVGIVGAVTGALPQLTAF
RNTQHIHVLDAVESIRTTVNRIRADVDLLIVAYHGGIECDMASGRPTQYDTGEDQAYRILSTIPGIDGLICGHQHRTNHG
ECHGIPYVQPGYQGNSVGFISYQFKERRISRHETAMLHPTTDKLEPLDPTTMNLKLDEYRTWLDQPIDTGRFGEYITLKT
GIRLQRFIWRKTSDGTTTIREFHHSFPKPYTASVFRLTWEELRTCIDQGLIQADMIPATEAPLGGYQVITNTPEAFPTYR
LERRTVDNLFDEYLHWLKQ

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=249, Percent_Identity=24.8995983935743, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 46673; Mature: 46542

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTITILATSDLHGFLPDTLADVPGDPSAANAHAAGILAAHAAKRMTGVDDATLLIDAGD
CCEEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCC
YLLGSAYATFTARSAERESPLTRVASACGYTAMALGNHDFDHGTALPASQNPHLHERLLC
HHHHCHHHHHEECCCHHCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHEEEE
CNVTDEEGHPIFHPYRLVQARGIRVGIVGAVTGALPQLTAFRNTQHIHVLDAVESIRTTV
EECCCCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
NRIRADVDLLIVAYHGGIECDMASGRPTQYDTGEDQAYRILSTIPGIDGLICGHQHRTNH
HHHHCCEEEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCC
GECHGIPYVQPGYQGNSVGFISYQFKERRISRHETAMLHPTTDKLEPLDPTTMNLKLDEY
CCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCEEECCCCCCCCCCCCCEEEEEHHHH
RTWLDQPIDTGRFGEYITLKTGIRLQRFIWRKTSDGTTTIREFHHSFPKPYTASVFRLTW
HHHHCCCCCCCCCCCEEEEECCCCEEHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
EELRTCIDQGLIQADMIPATEAPLGGYQVITNTPEAFPTYRLERRTVDNLFDEYLHWLKQ
HHHHHHHHHCCEECCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTITILATSDLHGFLPDTLADVPGDPSAANAHAAGILAAHAAKRMTGVDDATLLIDAGD
CEEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCC
YLLGSAYATFTARSAERESPLTRVASACGYTAMALGNHDFDHGTALPASQNPHLHERLLC
HHHHCHHHHHEECCCHHCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHEEEE
CNVTDEEGHPIFHPYRLVQARGIRVGIVGAVTGALPQLTAFRNTQHIHVLDAVESIRTTV
EECCCCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
NRIRADVDLLIVAYHGGIECDMASGRPTQYDTGEDQAYRILSTIPGIDGLICGHQHRTNH
HHHHCCEEEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCC
GECHGIPYVQPGYQGNSVGFISYQFKERRISRHETAMLHPTTDKLEPLDPTTMNLKLDEY
CCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCEEECCCCCCCCCCCCCEEEEEHHHH
RTWLDQPIDTGRFGEYITLKTGIRLQRFIWRKTSDGTTTIREFHHSFPKPYTASVFRLTW
HHHHCCCCCCCCCCCEEEEECCCCEEHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
EELRTCIDQGLIQADMIPATEAPLGGYQVITNTPEAFPTYRLERRTVDNLFDEYLHWLKQ
HHHHHHHHHCCEECCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]