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The map label for this gene is gyrB [H]

Identifier: 213690934

GI number: 213690934

Start: 5446

End: 7575

Strand: Direct

Name: gyrB [H]

Synonym: Blon_0006

Alternate gene names: 213690934

Gene position: 5446-7575 (Clockwise)

Preceding gene: 213690933

Following gene: 213690935

Centisome position: 0.19

GC content: 63.71

Gene sequence:

>2130_bases
ATGACGGAGGTTGACCCTGTAGTCACGGAAGGAAGCCTGTTGGCAGACGAAACCAGCAAAGAGGAGCAGCTGAAGGCGGC
TGCGGAACGCATCAACAATGCGGAATTGACCGAAGGCGAACTTGACGAATCCATGGCTCCCGAGCATTATGAGGCCTCTG
ATCTGAGGGTGCTCGAAGGACTTGAGGCCGTGCGTATCCGCCCCGGTATGTACATCGGTTCCACCGGGCCGCGCGGTTTG
CACCACCTGGTCTATGAGATCGTCGACAACTCGGTCGACGAGGCGCTGGCCGGATACGCCAGCCATATCGAGGTCTCCAT
TCTGCCGGACAACGGCATCCGCGTGGTGGACGATGGTCGAGGCATCCCGGTCGATGAGGTGCCCGGCGAGGGCGTCTCCG
GTGTGGAGACCGTGATGACCAAGCTGCACGCCGGCGGCAAGTTCGGCGGCGGCGGTTATGCAGTGTCCGGCGGTCTGCAC
GGCGTGGGCATCTCCGTGGTCAACGCACTCTCCACCCGCGTGGACATCGAAGTGCGCCGTCAGGGCTTCCACTGGACCCA
GACGTACATCGACCAGCACCCCACCGCGCCCTTGAAGCAGGGCGAGCCGATGACCGAAGGCGAGTCCACCGGCACGTCCG
TGACCTTCTGGGCCGACCCGAAGATTTTCGAGACCACCATCTACGACTTCGAGACGCTGCGCTCCCGCTTCCAGCAGATG
GCCTTCCTGAACAAGGGCCTCAAGATCAGCCTGACCGACGAACGCGAGAACGACCAGGCCGGCGACGAGGTGGCCGGCGA
CGCGGCGGACGAGCCGGGCGCGAAGCACCAGACCGTCACCTACCAATACCTCAACGGCATCAAGGACTACGTCGAGTATC
TGGTCAAGGTGCGCAAGGCCACGCCGGTGGAAGAGGACGTCATCAGCTTCGAGGCCGAAGACCTGAAGCTCGGCATCTCC
GCCGAACTCGCCATGCAGTGGACCACCGCCTACTCCGAGGCCGTGCACACCTTCGCGAACACGATTTCCACCACCGAGGG
CGGCACCCACGAGGAGGGCTTCCGCGCCGCGCTGACCTCGCTGGTCAACCGTTACGCGCGTGACAAGGGCATCCTCAAGG
ACAAGGACGAGAACCTCTCCGGCGACGACGTGCGTGAAGGCCTGACCGCCGTGATCTCCGTCAAGCTCACCAACCCGCAG
TTCGAAGGCCAGACCAAGACCAAGCTCGGCAACTCCGAGGCCAAGACCTTCGTGCAGCGCGTGATGACCGACAAGCTCGG
CGACTGGTTCGACGCCCACCCGAACGAGGCCAAGAACATCATCCAGAAGGCCATCGAGGCCTCCCGCGCCCGTCTGGCCG
CCAAGAAAGCCCGCGAGAACACCCGCCGCAAGTCCATCTTCGAGTCCGCCGGCATGCCCGACAAGCTCAAGGACTGCCAG
TCCTCCAACCCGGAAGAGTGCGAGCTGTTCATCGTGGAGGGCGATTCCGCAGGCGGTTCCGCCATCCAAGGCCGCAACCC
GATCACGCAGGCCATCCTGCCGCTGCGAGGCAAGATCCTCAACACCGAACGTGCCAGCTTGGACCGCATGATGAAGTCCG
ACACCATCGAATCGCTGATCACCGCGGTCGGCGGCGGCTATGGCGAGGACTTCGACATCTCCAAGGTCCGCTACCACAAG
GTCATCATCATGGCCGATGCCGATGTGGACGGTGCGCATATCGCCACCCTGAACCTGACGCTGTTCTTCCGCTACATGCG
CCCGATGATCACCGCCGGCTACGTGTACGTGGCCATGCCGCCGCTGTACCGCCTGAAGTGGACCAAGGGCCCGCACGACT
TCGTGTACACCGACGCCGAGCGCGACCGCGTGCTCGCCGAAGGCAAGGCCAACGGCCGCCAGCTGCCCAAGGGCGAAGGC
ATCCAGCGCTACAAGGGTCTGGGCGAGATGAGCTACCAGGAGCTATGGGAGACCACCATGGACCCCGAGCACCGCATTCT
GAAGCAGGTGCACATCGAGGACGCCGCCGCGGCCGACGAGACCTTCTCGATGCTGATGGGCGATGAGGTCGAACCCCGCC
GCCTCTTCATCCAGCGCAACGCCAAGAACGTGCGTTGGATCGATGCGTAA

Upstream 100 bases:

>100_bases
TCGTCGGGTTAGACGAGTAAAATCACATGCAGTATGGTCAAATGGCTAGAAGAGTCCTTTACGGGCCTCGTAAGCCATAT
CACCTCCGTGAATGACCTTC

Downstream 100 bases:

>100_bases
CGGAATCCTCCAACCTCCCTCGTCAGAGGGAGGTACACAACGGACTTAGACAATTAAAGGAACAACAGTGGCAGATGAAA
ACAACACCGGCGACGAGCAG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 709; Mature: 708

Protein sequence:

>709_residues
MTEVDPVVTEGSLLADETSKEEQLKAAAERINNAELTEGELDESMAPEHYEASDLRVLEGLEAVRIRPGMYIGSTGPRGL
HHLVYEIVDNSVDEALAGYASHIEVSILPDNGIRVVDDGRGIPVDEVPGEGVSGVETVMTKLHAGGKFGGGGYAVSGGLH
GVGISVVNALSTRVDIEVRRQGFHWTQTYIDQHPTAPLKQGEPMTEGESTGTSVTFWADPKIFETTIYDFETLRSRFQQM
AFLNKGLKISLTDERENDQAGDEVAGDAADEPGAKHQTVTYQYLNGIKDYVEYLVKVRKATPVEEDVISFEAEDLKLGIS
AELAMQWTTAYSEAVHTFANTISTTEGGTHEEGFRAALTSLVNRYARDKGILKDKDENLSGDDVREGLTAVISVKLTNPQ
FEGQTKTKLGNSEAKTFVQRVMTDKLGDWFDAHPNEAKNIIQKAIEASRARLAAKKARENTRRKSIFESAGMPDKLKDCQ
SSNPEECELFIVEGDSAGGSAIQGRNPITQAILPLRGKILNTERASLDRMMKSDTIESLITAVGGGYGEDFDISKVRYHK
VIIMADADVDGAHIATLNLTLFFRYMRPMITAGYVYVAMPPLYRLKWTKGPHDFVYTDAERDRVLAEGKANGRQLPKGEG
IQRYKGLGEMSYQELWETTMDPEHRILKQVHIEDAAAADETFSMLMGDEVEPRRLFIQRNAKNVRWIDA

Sequences:

>Translated_709_residues
MTEVDPVVTEGSLLADETSKEEQLKAAAERINNAELTEGELDESMAPEHYEASDLRVLEGLEAVRIRPGMYIGSTGPRGL
HHLVYEIVDNSVDEALAGYASHIEVSILPDNGIRVVDDGRGIPVDEVPGEGVSGVETVMTKLHAGGKFGGGGYAVSGGLH
GVGISVVNALSTRVDIEVRRQGFHWTQTYIDQHPTAPLKQGEPMTEGESTGTSVTFWADPKIFETTIYDFETLRSRFQQM
AFLNKGLKISLTDERENDQAGDEVAGDAADEPGAKHQTVTYQYLNGIKDYVEYLVKVRKATPVEEDVISFEAEDLKLGIS
AELAMQWTTAYSEAVHTFANTISTTEGGTHEEGFRAALTSLVNRYARDKGILKDKDENLSGDDVREGLTAVISVKLTNPQ
FEGQTKTKLGNSEAKTFVQRVMTDKLGDWFDAHPNEAKNIIQKAIEASRARLAAKKARENTRRKSIFESAGMPDKLKDCQ
SSNPEECELFIVEGDSAGGSAIQGRNPITQAILPLRGKILNTERASLDRMMKSDTIESLITAVGGGYGEDFDISKVRYHK
VIIMADADVDGAHIATLNLTLFFRYMRPMITAGYVYVAMPPLYRLKWTKGPHDFVYTDAERDRVLAEGKANGRQLPKGEG
IQRYKGLGEMSYQELWETTMDPEHRILKQVHIEDAAAADETFSMLMGDEVEPRRLFIQRNAKNVRWIDA
>Mature_708_residues
TEVDPVVTEGSLLADETSKEEQLKAAAERINNAELTEGELDESMAPEHYEASDLRVLEGLEAVRIRPGMYIGSTGPRGLH
HLVYEIVDNSVDEALAGYASHIEVSILPDNGIRVVDDGRGIPVDEVPGEGVSGVETVMTKLHAGGKFGGGGYAVSGGLHG
VGISVVNALSTRVDIEVRRQGFHWTQTYIDQHPTAPLKQGEPMTEGESTGTSVTFWADPKIFETTIYDFETLRSRFQQMA
FLNKGLKISLTDERENDQAGDEVAGDAADEPGAKHQTVTYQYLNGIKDYVEYLVKVRKATPVEEDVISFEAEDLKLGISA
ELAMQWTTAYSEAVHTFANTISTTEGGTHEEGFRAALTSLVNRYARDKGILKDKDENLSGDDVREGLTAVISVKLTNPQF
EGQTKTKLGNSEAKTFVQRVMTDKLGDWFDAHPNEAKNIIQKAIEASRARLAAKKARENTRRKSIFESAGMPDKLKDCQS
SNPEECELFIVEGDSAGGSAIQGRNPITQAILPLRGKILNTERASLDRMMKSDTIESLITAVGGGYGEDFDISKVRYHKV
IIMADADVDGAHIATLNLTLFFRYMRPMITAGYVYVAMPPLYRLKWTKGPHDFVYTDAERDRVLAEGKANGRQLPKGEGI
QRYKGLGEMSYQELWETTMDPEHRILKQVHIEDAAAADETFSMLMGDEVEPRRLFIQRNAKNVRWIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=678, Percent_Identity=25.6637168141593, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI19913406, Length=680, Percent_Identity=25.2941176470588, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI48994957, Length=563, Percent_Identity=47.6021314387211, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1789408, Length=654, Percent_Identity=38.3792048929664, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17535065, Length=675, Percent_Identity=23.1111111111111, Blast_Score=119, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=342, Percent_Identity=25.7309941520468, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=225, Percent_Identity=27.1111111111111, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=724, Percent_Identity=24.8618784530387, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136538, Length=700, Percent_Identity=22.4285714285714, Blast_Score=108, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 78321; Mature: 78190

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVDPVVTEGSLLADETSKEEQLKAAAERINNAELTEGELDESMAPEHYEASDLRVLEG
CCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHC
LEAVRIRPGMYIGSTGPRGLHHLVYEIVDNSVDEALAGYASHIEVSILPDNGIRVVDDGR
CHHEEECCCEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHCEEEEEECCCCCEEEECCC
GIPVDEVPGEGVSGVETVMTKLHAGGKFGGGGYAVSGGLHGVGISVVNALSTRVDIEVRR
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHEEEEECC
QGFHWTQTYIDQHPTAPLKQGEPMTEGESTGTSVTFWADPKIFETTIYDFETLRSRFQQM
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEHHHHHHHHHHHHHHHHH
AFLNKGLKISLTDERENDQAGDEVAGDAADEPGAKHQTVTYQYLNGIKDYVEYLVKVRKA
HHHCCCCEEEECCCCCCCCCCCHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHC
TPVEEDVISFEAEDLKLGISAELAMQWTTAYSEAVHTFANTISTTEGGTHEEGFRAALTS
CCCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LVNRYARDKGILKDKDENLSGDDVREGLTAVISVKLTNPQFEGQTKTKLGNSEAKTFVQR
HHHHHHHHCCCCCCCCCCCCCHHHHHCCEEEEEEEECCCCCCCCHHHHCCCHHHHHHHHH
VMTDKLGDWFDAHPNEAKNIIQKAIEASRARLAAKKARENTRRKSIFESAGMPDKLKDCQ
HHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHC
SSNPEECELFIVEGDSAGGSAIQGRNPITQAILPLRGKILNTERASLDRMMKSDTIESLI
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHH
TAVGGGYGEDFDISKVRYHKVIIMADADVDGAHIATLNLTLFFRYMRPMITAGYVYVAMP
HHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHCCEEEEECC
PLYRLKWTKGPHDFVYTDAERDRVLAEGKANGRQLPKGEGIQRYKGLGEMSYQELWETTM
CCEEEEECCCCCCCEEECCHHCHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
DPEHRILKQVHIEDAAAADETFSMLMGDEVEPRRLFIQRNAKNVRWIDA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCEEEECC
>Mature Secondary Structure 
TEVDPVVTEGSLLADETSKEEQLKAAAERINNAELTEGELDESMAPEHYEASDLRVLEG
CCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHC
LEAVRIRPGMYIGSTGPRGLHHLVYEIVDNSVDEALAGYASHIEVSILPDNGIRVVDDGR
CHHEEECCCEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHCEEEEEECCCCCEEEECCC
GIPVDEVPGEGVSGVETVMTKLHAGGKFGGGGYAVSGGLHGVGISVVNALSTRVDIEVRR
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHEEEEECC
QGFHWTQTYIDQHPTAPLKQGEPMTEGESTGTSVTFWADPKIFETTIYDFETLRSRFQQM
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEHHHHHHHHHHHHHHHHH
AFLNKGLKISLTDERENDQAGDEVAGDAADEPGAKHQTVTYQYLNGIKDYVEYLVKVRKA
HHHCCCCEEEECCCCCCCCCCCHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHC
TPVEEDVISFEAEDLKLGISAELAMQWTTAYSEAVHTFANTISTTEGGTHEEGFRAALTS
CCCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LVNRYARDKGILKDKDENLSGDDVREGLTAVISVKLTNPQFEGQTKTKLGNSEAKTFVQR
HHHHHHHHCCCCCCCCCCCCCHHHHHCCEEEEEEEECCCCCCCCHHHHCCCHHHHHHHHH
VMTDKLGDWFDAHPNEAKNIIQKAIEASRARLAAKKARENTRRKSIFESAGMPDKLKDCQ
HHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHC
SSNPEECELFIVEGDSAGGSAIQGRNPITQAILPLRGKILNTERASLDRMMKSDTIESLI
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHH
TAVGGGYGEDFDISKVRYHKVIIMADADVDGAHIATLNLTLFFRYMRPMITAGYVYVAMP
HHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHCCEEEEECC
PLYRLKWTKGPHDFVYTDAERDRVLAEGKANGRQLPKGEGIQRYKGLGEMSYQELWETTM
CCEEEEECCCCCCCEEECCHHCHHHHCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
DPEHRILKQVHIEDAAAADETFSMLMGDEVEPRRLFIQRNAKNVRWIDA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8392138 [H]