Click here to switch to the map view.

The map label for this gene is hgiDIIM [H]

Identifier: 213159140

GI number: 213159140

Start: 3972755

End: 3973822

Strand: Reverse

Name: hgiDIIM [H]

Synonym: AB57_3851

Alternate gene names: 213159140

Gene position: 3973822-3972755 (Counterclockwise)

Preceding gene: 213159141

Following gene: 213159139

Centisome position: 98.11

GC content: 35.86

Gene sequence:

>1068_bases
TTGAAAGTAATAGATTTCTTTTCTGGTTGCGGTGGGGCAAGTGAAGGACTACGTCAAGCTGGATTAGATATCACTATTGG
ACTCGATTTTGATATAAAGGCGGCTGAAACATATCAGGCTAATTTTCCTGAGGCATTATTCTATAATGTTGATATTAGAG
AATTAGATGAAAAAGAATTAGCAAAAGCTTTTAAAGAGAAAAACAGAGAAAAAGAACCACTTTTGTTTGTTGCATGTGCC
CCATGTCAGCCATTCTCTACACAAAATAAATCAAAAAGTGAAGATGATATTCGACGTACATTATTGGATGAAACACACAG
GTTTATCAGCAAATTAAAACCTGAATATATTCTTGTAGAAAATGTTCCTGGTCTTCAAAAAATTGATAAAGAAAAAGATG
GCCCTTACAAGCGGTTTATCACTTTTTTAGAATCTAAAAAATATAAGATTACAGAATTCATCGCAAAATCTGAAGAATAT
GGCGTCCCTCAAAAACGTAAACGTTTTGTTCTATTAGCCAGTAAATCTGGAAAAATAGAAATTCCAGCAAAAACACATGG
AGAAGGTTTAGAACCGATTAAAACAGTTCGCGATTTTATCGGAGAATTTCCAGCTATTAAAGCTGGTGAAATACATCCTG
ATGATTTATGGCATAGATCCCCGACCTTAAATGAACGTAATTTAGTGCGTATTCAAAATACCCCAGAAGGTGGGGATCGA
CGTCATTGGCCAAATCATTTGATTAATGAATGCCATAAGGCACACTCTGGACATATGGATGTGTATGGTCGTATGAGTTG
GGATAAACCAGCTCCAACCTTAACAACACGTTGTTATAGTTATTCAAATGGGCGTTTTGGCCATCCAGATACGAATCAGC
ATCGTGCAATTAGTGTGAGGGAAGCTTCAAGGTTACAAACATTCCCTCAAGACTTTATCTTTAAGGGAAGTGTAGTTGAG
GCTTCTAGGCAAATCGGAAATGCTGTGCCTTGTGAAATGGCTAAACAATTTGGTTTATCAATTCTTAAGCATTATGAAGA
AGTGAAAAATAAAAATGGCAAAGATTAA

Upstream 100 bases:

>100_bases
ATTAGAATTGGATGCAAAAGATTTACTTCCAAAATAATTTTAGAAACGTATCTTCTGTTATGATTTGGCATATTTTTTAA
ATATATAACTTGGTGTTGTT

Downstream 100 bases:

>100_bases
GACTCGTGCTCGTACTCTAGATATGTTAGGGCGTCAGCAAATTGCTGGTATTCCTACAGCATTAAGTGAGTTATTTAAAA
ATGCTCATGATGCTTATGCG

Product: DNA cytosine methyltransferase

Products: NA

Alternate protein names: M.HgiDII; Cytosine-specific methyltransferase HgiDII [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKVIDFFSGCGGASEGLRQAGLDITIGLDFDIKAAETYQANFPEALFYNVDIRELDEKELAKAFKEKNREKEPLLFVACA
PCQPFSTQNKSKSEDDIRRTLLDETHRFISKLKPEYILVENVPGLQKIDKEKDGPYKRFITFLESKKYKITEFIAKSEEY
GVPQKRKRFVLLASKSGKIEIPAKTHGEGLEPIKTVRDFIGEFPAIKAGEIHPDDLWHRSPTLNERNLVRIQNTPEGGDR
RHWPNHLINECHKAHSGHMDVYGRMSWDKPAPTLTTRCYSYSNGRFGHPDTNQHRAISVREASRLQTFPQDFIFKGSVVE
ASRQIGNAVPCEMAKQFGLSILKHYEEVKNKNGKD

Sequences:

>Translated_355_residues
MKVIDFFSGCGGASEGLRQAGLDITIGLDFDIKAAETYQANFPEALFYNVDIRELDEKELAKAFKEKNREKEPLLFVACA
PCQPFSTQNKSKSEDDIRRTLLDETHRFISKLKPEYILVENVPGLQKIDKEKDGPYKRFITFLESKKYKITEFIAKSEEY
GVPQKRKRFVLLASKSGKIEIPAKTHGEGLEPIKTVRDFIGEFPAIKAGEIHPDDLWHRSPTLNERNLVRIQNTPEGGDR
RHWPNHLINECHKAHSGHMDVYGRMSWDKPAPTLTTRCYSYSNGRFGHPDTNQHRAISVREASRLQTFPQDFIFKGSVVE
ASRQIGNAVPCEMAKQFGLSILKHYEEVKNKNGKD
>Mature_355_residues
MKVIDFFSGCGGASEGLRQAGLDITIGLDFDIKAAETYQANFPEALFYNVDIRELDEKELAKAFKEKNREKEPLLFVACA
PCQPFSTQNKSKSEDDIRRTLLDETHRFISKLKPEYILVENVPGLQKIDKEKDGPYKRFITFLESKKYKITEFIAKSEEY
GVPQKRKRFVLLASKSGKIEIPAKTHGEGLEPIKTVRDFIGEFPAIKAGEIHPDDLWHRSPTLNERNLVRIQNTPEGGDR
RHWPNHLINECHKAHSGHMDVYGRMSWDKPAPTLTTRCYSYSNGRFGHPDTNQHRAISVREASRLQTFPQDFIFKGSVVE
ASRQIGNAVPCEMAKQFGLSILKHYEEVKNKNGKD

Specific function: This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=186, Percent_Identity=25.8064516129032, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4503351, Length=186, Percent_Identity=25.8064516129032, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007318, Length=208, Percent_Identity=29.3269230769231, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 40556; Mature: 40556

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIDFFSGCGGASEGLRQAGLDITIGLDFDIKAAETYQANFPEALFYNVDIRELDEKEL
CCHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCHHCCCCCCEEEEECCHHHCCHHHH
AKAFKEKNREKEPLLFVACAPCQPFSTQNKSKSEDDIRRTLLDETHRFISKLKPEYILVE
HHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
NVPGLQKIDKEKDGPYKRFITFLESKKYKITEFIAKSEEYGVPQKRKRFVLLASKSGKIE
CCCCHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCEEEEEECCCCCEE
IPAKTHGEGLEPIKTVRDFIGEFPAIKAGEIHPDDLWHRSPTLNERNLVRIQNTPEGGDR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCC
RHWPNHLINECHKAHSGHMDVYGRMSWDKPAPTLTTRCYSYSNGRFGHPDTNQHRAISVR
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHEEECCCCCCCCCCCCCCCEEEHH
EASRLQTFPQDFIFKGSVVEASRQIGNAVPCEMAKQFGLSILKHYEEVKNKNGKD
HHHHHHHCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKVIDFFSGCGGASEGLRQAGLDITIGLDFDIKAAETYQANFPEALFYNVDIRELDEKEL
CCHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCHHCCCCCCEEEEECCHHHCCHHHH
AKAFKEKNREKEPLLFVACAPCQPFSTQNKSKSEDDIRRTLLDETHRFISKLKPEYILVE
HHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
NVPGLQKIDKEKDGPYKRFITFLESKKYKITEFIAKSEEYGVPQKRKRFVLLASKSGKIE
CCCCHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCEEEEEECCCCCEE
IPAKTHGEGLEPIKTVRDFIGEFPAIKAGEIHPDDLWHRSPTLNERNLVRIQNTPEGGDR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCC
RHWPNHLINECHKAHSGHMDVYGRMSWDKPAPTLTTRCYSYSNGRFGHPDTNQHRAISVR
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHEEECCCCCCCCCCCCCCCEEEHH
EASRLQTFPQDFIFKGSVVEASRQIGNAVPCEMAKQFGLSILKHYEEVKNKNGKD
HHHHHHHCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1937045; 7607523 [H]