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The map label for this gene is xth [H]

Identifier: 213159082

GI number: 213159082

Start: 3912198

End: 3913022

Strand: Direct

Name: xth [H]

Synonym: AB57_3792

Alternate gene names: 213159082

Gene position: 3912198-3913022 (Clockwise)

Preceding gene: 213159080

Following gene: 213159083

Centisome position: 96.59

GC content: 41.7

Gene sequence:

>825_bases
ATGATACCAAAGGATCAGTATCCAAGTGATGTAAAAATTCTTCGAGTCGTTTCCATTAACGTAAATGGCTTACGTTCTTC
AGTGACGAAAGGCTTACTTGAATGGTTAGAACAATCAGATGCCGATGTCGTATGCATGCAGGAAAGCCGCATTACCCATG
AACAATGGACTGAGAAATTTAGACCCGAGGGATGGCATACCCATCTCTTTCCAGCAGAGCGTGCAGGCTACGCAGGTACT
GCAATTTATAGCCGCTTACCCTTTGTCTCTATTAAAGATGGTTTAGGGTTTGAATTGGCCGATAGTCAAGGCCGCTTCAT
TTCTGCCGAATTCGATTTAGGTTTGTCACATCCCGTACATATTGCATCATTATACTTACCTTCAGGCTCAAGTGGTGAAG
AAGCGCAAGCACGTAAAGATTTATTTTTAGGTGAATACGCCAAAATCTTGAAACAATGGCGTGATGAGAATAAGTCCATC
ATTATTTGTGGCGATTACAACATTGTTCATAAACGGATTGATATTAAAAACTGGTCTGGCAACCAAAAGTCGTCTGGCTG
TTTACCACATGAGCGTGCTTGGCTTGACCACATTTATGATGAACTTGGTTATGTAGATACTTTCCGTGTTGTAAGAACAG
AAGCGGAATTGTATTCATGGTGGTCAAATCGCGGACAAGCGCGTGCGAAAAATGTAGGTTGGCGTATTGACTATCACGCA
TGTTCACCAGACTGGAAAGCGCGCACTGTAAATGCATGGGTCTATAAAGATCAATGGTTTAGTGACCATGCTCCAGTTAT
TATCGACTATAAAATTCAAGAATAA

Upstream 100 bases:

>100_bases
ACACCGCGTGATAATGCGAGTTCGATAAATGCTTGCGGGTGAAATGACACAGGCGTTGTCATGAGGTGCTCCAAATTCAC
AATTGAGGTTAAATTTTCGC

Downstream 100 bases:

>100_bases
GTGAACACATGTTCACTTACATTGTCGTTTTTGCTTACAACACATGTCGCTTTTGAGTATTTTTCTTACACATGTTTAAC
GGCATTATAGCCCCACGGCA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MIPKDQYPSDVKILRVVSINVNGLRSSVTKGLLEWLEQSDADVVCMQESRITHEQWTEKFRPEGWHTHLFPAERAGYAGT
AIYSRLPFVSIKDGLGFELADSQGRFISAEFDLGLSHPVHIASLYLPSGSSGEEAQARKDLFLGEYAKILKQWRDENKSI
IICGDYNIVHKRIDIKNWSGNQKSSGCLPHERAWLDHIYDELGYVDTFRVVRTEAELYSWWSNRGQARAKNVGWRIDYHA
CSPDWKARTVNAWVYKDQWFSDHAPVIIDYKIQE

Sequences:

>Translated_274_residues
MIPKDQYPSDVKILRVVSINVNGLRSSVTKGLLEWLEQSDADVVCMQESRITHEQWTEKFRPEGWHTHLFPAERAGYAGT
AIYSRLPFVSIKDGLGFELADSQGRFISAEFDLGLSHPVHIASLYLPSGSSGEEAQARKDLFLGEYAKILKQWRDENKSI
IICGDYNIVHKRIDIKNWSGNQKSSGCLPHERAWLDHIYDELGYVDTFRVVRTEAELYSWWSNRGQARAKNVGWRIDYHA
CSPDWKARTVNAWVYKDQWFSDHAPVIIDYKIQE
>Mature_274_residues
MIPKDQYPSDVKILRVVSINVNGLRSSVTKGLLEWLEQSDADVVCMQESRITHEQWTEKFRPEGWHTHLFPAERAGYAGT
AIYSRLPFVSIKDGLGFELADSQGRFISAEFDLGLSHPVHIASLYLPSGSSGEEAQARKDLFLGEYAKILKQWRDENKSI
IICGDYNIVHKRIDIKNWSGNQKSSGCLPHERAWLDHIYDELGYVDTFRVVRTEAELYSWWSNRGQARAKNVGWRIDYHA
CSPDWKARTVNAWVYKDQWFSDHAPVIIDYKIQE

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=273, Percent_Identity=28.5714285714286, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI18375503, Length=273, Percent_Identity=28.5714285714286, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI18375501, Length=273, Percent_Identity=28.5714285714286, Blast_Score=133, Evalue=1e-31,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=27.3408239700375, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71989536, Length=260, Percent_Identity=30, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI221330655, Length=275, Percent_Identity=32.7272727272727, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17136678, Length=275, Percent_Identity=32.7272727272727, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31607; Mature: 31607

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPKDQYPSDVKILRVVSINVNGLRSSVTKGLLEWLEQSDADVVCMQESRITHEQWTEKF
CCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
RPEGWHTHLFPAERAGYAGTAIYSRLPFVSIKDGLGFELADSQGRFISAEFDLGLSHPVH
CCCCCEEECCCHHHCCCCHHHHHHHCCEEEECCCCCEEEECCCCCEEEEEECCCCCCCEE
IASLYLPSGSSGEEAQARKDLFLGEYAKILKQWRDENKSIIICGDYNIVHKRIDIKNWSG
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCC
NQKSSGCLPHERAWLDHIYDELGYVDTFRVVRTEAELYSWWSNRGQARAKNVGWRIDYHA
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
CSPDWKARTVNAWVYKDQWFSDHAPVIIDYKIQE
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MIPKDQYPSDVKILRVVSINVNGLRSSVTKGLLEWLEQSDADVVCMQESRITHEQWTEKF
CCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
RPEGWHTHLFPAERAGYAGTAIYSRLPFVSIKDGLGFELADSQGRFISAEFDLGLSHPVH
CCCCCEEECCCHHHCCCCHHHHHHHCCEEEECCCCCEEEECCCCCEEEEEECCCCCCCEE
IASLYLPSGSSGEEAQARKDLFLGEYAKILKQWRDENKSIIICGDYNIVHKRIDIKNWSG
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCC
NQKSSGCLPHERAWLDHIYDELGYVDTFRVVRTEAELYSWWSNRGQARAKNVGWRIDYHA
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
CSPDWKARTVNAWVYKDQWFSDHAPVIIDYKIQE
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]