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The map label for this gene is yddQ [H]

Identifier: 213159047

GI number: 213159047

Start: 3872204

End: 3872746

Strand: Direct

Name: yddQ [H]

Synonym: AB57_3755

Alternate gene names: 213159047

Gene position: 3872204-3872746 (Clockwise)

Preceding gene: 213159045

Following gene: 213159049

Centisome position: 95.6

GC content: 35.54

Gene sequence:

>543_bases
ATGAAACAAGCACTATTAGTTATCGATGTACAAAATGATTATTTTAAAAATGGCAAAATGGAATTGGTTGGACCAGATCA
AGCGCTCGATAAAATAAAACAGCTCGAGCAATATTTTAATGAAAAAGGTTTACCGATTATTTATGTGCAACATATTAACC
CGCCACAAGCCAGCTTCTTTCAAGAAAATACTGAAGGTGTGTTACTTCATCCAGAACTTAGTGCTGGCAGTAATTCATTA
ATCGTGACAAAGCATTATCCAAACAGTTTTTTAGAAACCAATTTAAAAGAACTTTTAAAAGCACATCAAATTGAACAACT
CGTGATCACAGGCATGATGACACATATTTGTATTGATTCTGGCACACGTGCAGCCAAAGAGTTAGGTTATCAACCCATCT
TAATTGGAGATACCACTGCGACCCGTGATTTAAGCCATAACGGAAAAACTGTTAAAGCCGAAGATGTACAAACTGCTTTT
TTAGCTGCTCTTGGCATGTTTGCAAGCGTACAAAATACTGCTGAATTTCTAGCAAAATTTTAA

Upstream 100 bases:

>100_bases
ATAACGAAAGAAAACTGGCGATAATGCTTTCATAGGACTATAGCCGATTTTGCGGGCAGAGAATAACCTTGTTTTATCTT
ATACCAACAAGAGTATTCTC

Downstream 100 bases:

>100_bases
ACAGGTTTAATAAAAAAGCCCCTAACGGGGCTTTTTTATTAAAACGTTATTTTTGTCTATCAGGATAAACTGTCGCAATC
AGTTGATCCACCACTGCCGG

Product: amidase

Products: Nicotinate; pyrazinoic acid; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MKQALLVIDVQNDYFKNGKMELVGPDQALDKIKQLEQYFNEKGLPIIYVQHINPPQASFFQENTEGVLLHPELSAGSNSL
IVTKHYPNSFLETNLKELLKAHQIEQLVITGMMTHICIDSGTRAAKELGYQPILIGDTTATRDLSHNGKTVKAEDVQTAF
LAALGMFASVQNTAEFLAKF

Sequences:

>Translated_180_residues
MKQALLVIDVQNDYFKNGKMELVGPDQALDKIKQLEQYFNEKGLPIIYVQHINPPQASFFQENTEGVLLHPELSAGSNSL
IVTKHYPNSFLETNLKELLKAHQIEQLVITGMMTHICIDSGTRAAKELGYQPILIGDTTATRDLSHNGKTVKAEDVQTAF
LAALGMFASVQNTAEFLAKF
>Mature_180_residues
MKQALLVIDVQNDYFKNGKMELVGPDQALDKIKQLEQYFNEKGLPIIYVQHINPPQASFFQENTEGVLLHPELSAGSNSL
IVTKHYPNSFLETNLKELLKAHQIEQLVITGMMTHICIDSGTRAAKELGYQPILIGDTTATRDLSHNGKTVKAEDVQTAF
LAALGMFASVQNTAEFLAKF

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 20005; Mature: 20005

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQALLVIDVQNDYFKNGKMELVGPDQALDKIKQLEQYFNEKGLPIIYVQHINPPQASFF
CCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
QENTEGVLLHPELSAGSNSLIVTKHYPNSFLETNLKELLKAHQIEQLVITGMMTHICIDS
HCCCCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTRAAKELGYQPILIGDTTATRDLSHNGKTVKAEDVQTAFLAALGMFASVQNTAEFLAKF
CCHHHHHCCCCCEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQALLVIDVQNDYFKNGKMELVGPDQALDKIKQLEQYFNEKGLPIIYVQHINPPQASFF
CCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
QENTEGVLLHPELSAGSNSLIVTKHYPNSFLETNLKELLKAHQIEQLVITGMMTHICIDS
HCCCCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTRAAKELGYQPILIGDTTATRDLSHNGKTVKAEDVQTAFLAALGMFASVQNTAEFLAKF
CCHHHHHCCCCCEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nicotinamide; pyrazinamide; H2O [C]

Specific reaction: Nicotinamide + H2O = Nicotinate + NH3 pyrazinamide + H2O = pyrazinoic acid + NH3 [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]