Click here to switch to the map view.

The map label for this gene is ynfL [H]

Identifier: 213159010

GI number: 213159010

Start: 3828431

End: 3829324

Strand: Reverse

Name: ynfL [H]

Synonym: AB57_3717

Alternate gene names: 213159010

Gene position: 3829324-3828431 (Counterclockwise)

Preceding gene: 213159013

Following gene: 213159009

Centisome position: 94.54

GC content: 44.07

Gene sequence:

>894_bases
TTGGCAGTAGCAGAAGAAAAAAACTTTACCAAAGCTGCGCAACGCTTAAACATGAGCCAGCCACCTTTAAGCATGCAAAT
ACGAGATTTAGAAGAAGAGCTGGGTGCAGATTTATTTATTCGATCACCTCATGGGGTGGAGCTGACCGAAGCAGGGCTTG
CCTTTTTAAATGCCATTCAGCCTGTACAGCAACGTGTAGAAGATGCAGCGAGCTTAGTTAAACAAGTGGCAAATGGCGAA
GTGGGCCAATTGCGATTAGGTTTTACAGGCACTTCAATGTTGAATCCTTTAATTCCTAAATGTGTTCGCTATTTCCAGCA
GCAATACCCGAAAGTGAATTTAAAGCTTGAAGAAGCCAATACCTTACTACTCATCGATTCATTGCTTGAAGACCGCTTAG
ATGTCGCAATTATTCGTTCGCCACGCAATATGCCGGACAGTCTTATACTTCAAGAATTATTGGCTGAACCACTCATTGCT
GCTTTACCTTCCGAGTCTTTTCAACTCGACGATGTATCACAAGAAATTGATTTAACTGCGCTACGAGAGCTTGATTTTAT
TGTCTCACCACCTTCAGTGAGTGCAGGGCTTTTTGATGCGATTAAACAGGCCTGCCATGAGCGTGGCTTTGAACCGAAAA
TTGGACAAAATGCCCCGCAAATTGTGTCAATTTTATCGTTGGTGTCTGCAAACTTAGGGGTGTCTTTGGTTCCCGAGTCG
ACCAAACAATTGCAAATACAAGGTGTGGCTTATCGGTCTTTAAAAGCACCTGTCCCTACGGTGGGTTTAGGGCTTGCCTA
TAAAAAACATGCCCCTTCACAAATGGCGATTAACTTTGCCAGTGTGGTGCAAGTGGCTTGCCACCACGCCAACGAAATAA
ATGAAATCGGCTAG

Upstream 100 bases:

>100_bases
TTAAAATTAGCAATTTGAGAGGTATTTTTCTAATATATTTAACAATGGATCATAATATGTTTTATATATAGCAATGGAAC
TCAGGCATATACGCTACTTT

Downstream 100 bases:

>100_bases
TTTGATTTCGGCGCTCTGTTCATTAAAAAGATGCCTGAAATAATAAAAATCGCGCCATATAAAAACGATAAAGTCGGCTG
TTCATGAATGAAAACCCAAG

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAVAEEKNFTKAAQRLNMSQPPLSMQIRDLEEELGADLFIRSPHGVELTEAGLAFLNAIQPVQQRVEDAASLVKQVANGE
VGQLRLGFTGTSMLNPLIPKCVRYFQQQYPKVNLKLEEANTLLLIDSLLEDRLDVAIIRSPRNMPDSLILQELLAEPLIA
ALPSESFQLDDVSQEIDLTALRELDFIVSPPSVSAGLFDAIKQACHERGFEPKIGQNAPQIVSILSLVSANLGVSLVPES
TKQLQIQGVAYRSLKAPVPTVGLGLAYKKHAPSQMAINFASVVQVACHHANEINEIG

Sequences:

>Translated_297_residues
MAVAEEKNFTKAAQRLNMSQPPLSMQIRDLEEELGADLFIRSPHGVELTEAGLAFLNAIQPVQQRVEDAASLVKQVANGE
VGQLRLGFTGTSMLNPLIPKCVRYFQQQYPKVNLKLEEANTLLLIDSLLEDRLDVAIIRSPRNMPDSLILQELLAEPLIA
ALPSESFQLDDVSQEIDLTALRELDFIVSPPSVSAGLFDAIKQACHERGFEPKIGQNAPQIVSILSLVSANLGVSLVPES
TKQLQIQGVAYRSLKAPVPTVGLGLAYKKHAPSQMAINFASVVQVACHHANEINEIG
>Mature_296_residues
AVAEEKNFTKAAQRLNMSQPPLSMQIRDLEEELGADLFIRSPHGVELTEAGLAFLNAIQPVQQRVEDAASLVKQVANGEV
GQLRLGFTGTSMLNPLIPKCVRYFQQQYPKVNLKLEEANTLLLIDSLLEDRLDVAIIRSPRNMPDSLILQELLAEPLIAA
LPSESFQLDDVSQEIDLTALRELDFIVSPPSVSAGLFDAIKQACHERGFEPKIGQNAPQIVSILSLVSANLGVSLVPEST
KQLQIQGVAYRSLKAPVPTVGLGLAYKKHAPSQMAINFASVVQVACHHANEINEIG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=280, Percent_Identity=35, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI2367136, Length=262, Percent_Identity=34.3511450381679, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=31.8181818181818, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI157672245, Length=264, Percent_Identity=30.6818181818182, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787601, Length=238, Percent_Identity=26.890756302521, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790399, Length=282, Percent_Identity=27.3049645390071, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788297, Length=235, Percent_Identity=25.9574468085106, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082132, Length=250, Percent_Identity=29.2, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787806, Length=143, Percent_Identity=33.5664335664336, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=184, Percent_Identity=27.7173913043478, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787589, Length=116, Percent_Identity=32.7586206896552, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=23.5537190082645, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=238, Percent_Identity=24.7899159663866, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789506, Length=151, Percent_Identity=27.1523178807947, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787530, Length=144, Percent_Identity=27.7777777777778, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32376; Mature: 32245

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAEEKNFTKAAQRLNMSQPPLSMQIRDLEEELGADLFIRSPHGVELTEAGLAFLNAIQ
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHH
PVQQRVEDAASLVKQVANGEVGQLRLGFTGTSMLNPLIPKCVRYFQQQYPKVNLKLEEAN
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
TLLLIDSLLEDRLDVAIIRSPRNMPDSLILQELLAEPLIAALPSESFQLDDVSQEIDLTA
EEEEHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LRELDFIVSPPSVSAGLFDAIKQACHERGFEPKIGQNAPQIVSILSLVSANLGVSLVPES
HHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCC
TKQLQIQGVAYRSLKAPVPTVGLGLAYKKHAPSQMAINFASVVQVACHHANEINEIG
CHHEEEEHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVAEEKNFTKAAQRLNMSQPPLSMQIRDLEEELGADLFIRSPHGVELTEAGLAFLNAIQ
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHH
PVQQRVEDAASLVKQVANGEVGQLRLGFTGTSMLNPLIPKCVRYFQQQYPKVNLKLEEAN
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
TLLLIDSLLEDRLDVAIIRSPRNMPDSLILQELLAEPLIAALPSESFQLDDVSQEIDLTA
EEEEHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LRELDFIVSPPSVSAGLFDAIKQACHERGFEPKIGQNAPQIVSILSLVSANLGVSLVPES
HHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCC
TKQLQIQGVAYRSLKAPVPTVGLGLAYKKHAPSQMAINFASVVQVACHHANEINEIG
CHHEEEEHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]