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The map label for this gene is nucA [H]

Identifier: 213158995

GI number: 213158995

Start: 3814012

End: 3815091

Strand: Reverse

Name: nucA [H]

Synonym: AB57_3700

Alternate gene names: 213158995

Gene position: 3815091-3814012 (Counterclockwise)

Preceding gene: 213158997

Following gene: 213158994

Centisome position: 94.19

GC content: 40.83

Gene sequence:

>1080_bases
ATGGCAAGTAAAAAACGTAGTAAAGGTACAAATTCATTTTCTCTAAGTGAGCATTTTGGCAAGATTATTCTTGCTTGTAT
TGCAGCCGGAAGCTTTGCAATTGCTTTCGGCAGTGAAAAGATTTCGCAGTGGTTTTCGCCTTTAAGTACTAACTCGACTT
GTCTTAACCAGTTTTATCGTGAAGTTCCGCCTGCGTTAAATAAAGAAAGCTTAAAAAAAGACAGCTATCCGCTGTGCTTT
AACGGTTTTAATGTCCTCTATTCTGGCATTTCTAAAACACCATTATGGTCAGCCGAGCATTTAGATGCAGAACGTTTAAG
TGTCAAAATTAAACGTGAAGATAACTTCCATGAAGAAACGCGTGTTCCTCAAAAGCACCGGGCGTTGTTAAGTGACTACC
GTGGTTCAGGGTATGACCGTGGGCACATGGCACCAAATGGCGATATGCCAAATAAAGAATCACAGTCTGATAGTTTCTCG
CTCAGCAACATGGTGCCGCAAGCGCCTAAAAATAACCAAGAAGTCTGGCGTAAACTTGAAGAAGCAACCCGTGCCATTGT
CACCAAGCAAAAACAAGATGTATATGTCGTGACAGGACCTGTTTTTGAGGGTAAACGTCTCAAAACGATTGGGCAGGGTG
TGATTGTACCTACCGCAGTTTATAAAGCGGTGTATATGCCAAAAACTGGCGCTATTGGGGCTTACTACGCACCTAACAAT
AACTCGCAGCAAGTGAAGGTTGTGAGTGTTTGTTATATAGAAGAAAAGCTTGGTATTAACTTATTTCCACAGCTTACAGA
ACAGCAAAAACGTAACGTTTATCGCTTACCGTTAACTGCTAGCCAAGTTAAACCTAACCAAAAGCTCGACTATTTGCATT
GGGATGGTGAAAGCCAGTGCGAACAAGACCTAAGCGCAGAACAAATTCAAGCGTTACAAGATCAGTTTAAAAAGCAAAAA
ACTGGTTCTTCTGAACCGATGGAAGCAAAAGTTCCAAGTATTGATGAAGAAACTAGAAATGCCATTGTGAAACAATTGGT
TGAAGCCTTGGTGAACTATTTCTTGCAGATAATGAAATAA

Upstream 100 bases:

>100_bases
ACAAAAAAGATGACCACGTTTTAACCATTTGAAAGATAAGCGATATACTTAAAAAAGCAGGATTGTTAAGCTGCGTGTGG
ACAGTGGTATTGATAGAAAC

Downstream 100 bases:

>100_bases
GACGCTTCTAGCACTGTTGTGAAGCCTGAATCGATACAATAAGATGAACAGACAGATCTGTCTGTTCATCTATTTATTCG
AATAAGGATAATAACAATGT

Product: DNA/RNA non-specific endonuclease G protein

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MASKKRSKGTNSFSLSEHFGKIILACIAAGSFAIAFGSEKISQWFSPLSTNSTCLNQFYREVPPALNKESLKKDSYPLCF
NGFNVLYSGISKTPLWSAEHLDAERLSVKIKREDNFHEETRVPQKHRALLSDYRGSGYDRGHMAPNGDMPNKESQSDSFS
LSNMVPQAPKNNQEVWRKLEEATRAIVTKQKQDVYVVTGPVFEGKRLKTIGQGVIVPTAVYKAVYMPKTGAIGAYYAPNN
NSQQVKVVSVCYIEEKLGINLFPQLTEQQKRNVYRLPLTASQVKPNQKLDYLHWDGESQCEQDLSAEQIQALQDQFKKQK
TGSSEPMEAKVPSIDEETRNAIVKQLVEALVNYFLQIMK

Sequences:

>Translated_359_residues
MASKKRSKGTNSFSLSEHFGKIILACIAAGSFAIAFGSEKISQWFSPLSTNSTCLNQFYREVPPALNKESLKKDSYPLCF
NGFNVLYSGISKTPLWSAEHLDAERLSVKIKREDNFHEETRVPQKHRALLSDYRGSGYDRGHMAPNGDMPNKESQSDSFS
LSNMVPQAPKNNQEVWRKLEEATRAIVTKQKQDVYVVTGPVFEGKRLKTIGQGVIVPTAVYKAVYMPKTGAIGAYYAPNN
NSQQVKVVSVCYIEEKLGINLFPQLTEQQKRNVYRLPLTASQVKPNQKLDYLHWDGESQCEQDLSAEQIQALQDQFKKQK
TGSSEPMEAKVPSIDEETRNAIVKQLVEALVNYFLQIMK
>Mature_358_residues
ASKKRSKGTNSFSLSEHFGKIILACIAAGSFAIAFGSEKISQWFSPLSTNSTCLNQFYREVPPALNKESLKKDSYPLCFN
GFNVLYSGISKTPLWSAEHLDAERLSVKIKREDNFHEETRVPQKHRALLSDYRGSGYDRGHMAPNGDMPNKESQSDSFSL
SNMVPQAPKNNQEVWRKLEEATRAIVTKQKQDVYVVTGPVFEGKRLKTIGQGVIVPTAVYKAVYMPKTGAIGAYYAPNNN
SQQVKVVSVCYIEEKLGINLFPQLTEQQKRNVYRLPLTASQVKPNQKLDYLHWDGESQCEQDLSAEQIQALQDQFKKQKT
GSSEPMEAKVPSIDEETRNAIVKQLVEALVNYFLQIMK

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI53759134, Length=213, Percent_Identity=33.8028169014084, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI224451073, Length=233, Percent_Identity=30.4721030042918, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI224451075, Length=186, Percent_Identity=32.7956989247312, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17505967, Length=239, Percent_Identity=28.4518828451883, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322253, Length=218, Percent_Identity=30.2752293577982, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI20129899, Length=206, Percent_Identity=31.0679611650485, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24581065, Length=179, Percent_Identity=29.608938547486, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: 3.1.30.-

Molecular weight: Translated: 40442; Mature: 40311

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKKRSKGTNSFSLSEHFGKIILACIAAGSFAIAFGSEKISQWFSPLSTNSTCLNQFYR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCHHHHHHHHH
EVPPALNKESLKKDSYPLCFNGFNVLYSGISKTPLWSAEHLDAERLSVKIKREDNFHEET
HCCCCCCHHHHCCCCCCEEECCHHHHHCCCCCCCCCCHHHCCHHHHEEEEECCCCCHHHH
RVPQKHRALLSDYRGSGYDRGHMAPNGDMPNKESQSDSFSLSNMVPQAPKNNQEVWRKLE
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHH
EATRAIVTKQKQDVYVVTGPVFEGKRLKTIGQGVIVPTAVYKAVYMPKTGAIGAYYAPNN
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCCC
NSQQVKVVSVCYIEEKLGINLFPQLTEQQKRNVYRLPLTASQVKPNQKLDYLHWDGESQC
CCCEEEEEEEEEEHHHCCCCCCHHHHHHHHCCEEECCCCHHHCCCCCCCCEEECCCCHHH
EQDLSAEQIQALQDQFKKQKTGSSEPMEAKVPSIDEETRNAIVKQLVEALVNYFLQIMK
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ASKKRSKGTNSFSLSEHFGKIILACIAAGSFAIAFGSEKISQWFSPLSTNSTCLNQFYR
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCHHHHHHHHH
EVPPALNKESLKKDSYPLCFNGFNVLYSGISKTPLWSAEHLDAERLSVKIKREDNFHEET
HCCCCCCHHHHCCCCCCEEECCHHHHHCCCCCCCCCCHHHCCHHHHEEEEECCCCCHHHH
RVPQKHRALLSDYRGSGYDRGHMAPNGDMPNKESQSDSFSLSNMVPQAPKNNQEVWRKLE
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHH
EATRAIVTKQKQDVYVVTGPVFEGKRLKTIGQGVIVPTAVYKAVYMPKTGAIGAYYAPNN
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCCC
NSQQVKVVSVCYIEEKLGINLFPQLTEQQKRNVYRLPLTASQVKPNQKLDYLHWDGESQC
CCCEEEEEEEEEEHHHCCCCCCHHHHHHHHCCEEECCCCHHHCCCCCCCCEEECCCCHHH
EQDLSAEQIQALQDQFKKQKTGSSEPMEAKVPSIDEETRNAIVKQLVEALVNYFLQIMK
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]