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The map label for this gene is trmD

Identifier: 213158915

GI number: 213158915

Start: 3722318

End: 3723058

Strand: Reverse

Name: trmD

Synonym: AB57_3616

Alternate gene names: 213158915

Gene position: 3723058-3722318 (Counterclockwise)

Preceding gene: 213158916

Following gene: 213158914

Centisome position: 91.92

GC content: 43.45

Gene sequence:

>741_bases
GTGTTTTTTGCAGTCATTACGCTTTTTCCTGAAATGTTTGACGCGATTACAGCCTACGGGATTAGCGGGCGTGCAGCAAA
ACGTGACATAGTACAAGTCACTTGTATTAATCCACGTGATTTTGCTGAAGGGAATTACCGAAGAGTGGATGAACGTCCAT
TTGGTGGTGGCCCTGGTATGGTGATGATGGCGGAGCCTTTGGCAAAAGCAATTAACCATGCCAAACAACTTGCTTCGCGA
GCAGGATGTGTTCATGTACCTGTGGTGTATATGTCTCCACAAGGCAAAACTTTAAATGAACAGGCAGTACAGCAGTTTGT
CGATTATGATGGCTTAATTGTATTGTGTGGGCGTTATGAAGGCGTCGATGAGCGTTTGATCCAGCATTATGTTGATCAGG
AATGGTCGATTGGAGATTACGTCTTATCTGGTGGTGAACTACCTGCCATGGTGTTGCTTGATAGTATTATTCGCCGTTTG
CCGAATGTAATGTCAGATGAGCAATCAGCGATACAGGATTCTTTTGTAGATGGTCTTTTGGATTGTCCGCAATACACTAA
GCCAGACCAGTTTGAAGGGTTAGATGTTCCTGAAATTTTAAAGTCGGGACATCATGCCAATATTGAGAAATGGCGTTTTT
TGCAGCGTTATCAACGAACTTTAGAGCGTCGACCTGAGTTGATTGAACAGGTTACGTTGACTAAGCAGCAAAAAAAATGG
CTGTCAGATGAACAAGGGTAA

Upstream 100 bases:

>100_bases
ACGTGTTGATCTTGAAGCTGGTCGCATTTACGTCAATTGGGGCGTAGATTATTAATCCTTTTTATAGGATTAACGCAGCA
GGAAAATAGAGGAGTCTGCG

Downstream 100 bases:

>100_bases
GCATATTCCCTGTGTTTTTCTGAGCAAAAGATGAGAAGCTTTGCTTCGAATTAAAGCATAAGTCTATTTTTTATAGATTT
ATGTGTACTTAAGGCTCTTT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MFFAVITLFPEMFDAITAYGISGRAAKRDIVQVTCINPRDFAEGNYRRVDERPFGGGPGMVMMAEPLAKAINHAKQLASR
AGCVHVPVVYMSPQGKTLNEQAVQQFVDYDGLIVLCGRYEGVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRL
PNVMSDEQSAIQDSFVDGLLDCPQYTKPDQFEGLDVPEILKSGHHANIEKWRFLQRYQRTLERRPELIEQVTLTKQQKKW
LSDEQG

Sequences:

>Translated_246_residues
MFFAVITLFPEMFDAITAYGISGRAAKRDIVQVTCINPRDFAEGNYRRVDERPFGGGPGMVMMAEPLAKAINHAKQLASR
AGCVHVPVVYMSPQGKTLNEQAVQQFVDYDGLIVLCGRYEGVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRL
PNVMSDEQSAIQDSFVDGLLDCPQYTKPDQFEGLDVPEILKSGHHANIEKWRFLQRYQRTLERRPELIEQVTLTKQQKKW
LSDEQG
>Mature_246_residues
MFFAVITLFPEMFDAITAYGISGRAAKRDIVQVTCINPRDFAEGNYRRVDERPFGGGPGMVMMAEPLAKAINHAKQLASR
AGCVHVPVVYMSPQGKTLNEQAVQQFVDYDGLIVLCGRYEGVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRL
PNVMSDEQSAIQDSFVDGLLDCPQYTKPDQFEGLDVPEILKSGHHANIEKWRFLQRYQRTLERRPELIEQVTLTKQQKKW
LSDEQG

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=243, Percent_Identity=51.8518518518518, Blast_Score=266, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_ACIB3 (B7GVS0)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002324283.1
- ProteinModelPortal:   B7GVS0
- SMR:   B7GVS0
- GeneID:   7058187
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_000350
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27895; Mature: 27895

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFAVITLFPEMFDAITAYGISGRAAKRDIVQVTCINPRDFAEGNYRRVDERPFGGGPGM
CHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEECCCHHHCCCCCEECCCCCCCCCCCC
VMMAEPLAKAINHAKQLASRAGCVHVPVVYMSPQGKTLNEQAVQQFVDYDGLIVLCGRYE
EEHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCC
GVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRLPNVMSDEQSAIQDSFVDGLL
CHHHHHHHHHHCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DCPQYTKPDQFEGLDVPEILKSGHHANIEKWRFLQRYQRTLERRPELIEQVTLTKQQKKW
CCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSDEQG
CCCCCC
>Mature Secondary Structure
MFFAVITLFPEMFDAITAYGISGRAAKRDIVQVTCINPRDFAEGNYRRVDERPFGGGPGM
CHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEECCCHHHCCCCCEECCCCCCCCCCCC
VMMAEPLAKAINHAKQLASRAGCVHVPVVYMSPQGKTLNEQAVQQFVDYDGLIVLCGRYE
EEHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCC
GVDERLIQHYVDQEWSIGDYVLSGGELPAMVLLDSIIRRLPNVMSDEQSAIQDSFVDGLL
CHHHHHHHHHHCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DCPQYTKPDQFEGLDVPEILKSGHHANIEKWRFLQRYQRTLERRPELIEQVTLTKQQKKW
CCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSDEQG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA