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The map label for this gene is truB [H]

Identifier: 213158911

GI number: 213158911

Start: 3718544

End: 3719449

Strand: Reverse

Name: truB [H]

Synonym: AB57_3612

Alternate gene names: 213158911

Gene position: 3719449-3718544 (Counterclockwise)

Preceding gene: 213158914

Following gene: 213158910

Centisome position: 91.83

GC content: 39.4

Gene sequence:

>906_bases
ATGACGACAAAAAATCAAAAAATTTCTCGTCGCCATATCAGTGGTGTATTTCTTTTAAATAAACCATTAGGGCTAAGTTC
TAACGCTGCTTTGCAAAAAGTGCGTTGGCTTTACCGTGCCCAAAAGGCGGGTCATACTGGTGCATTAGATCCATTAGCAA
CCGGTTTACTTCCTATTTGTTTAGGAGAGGCCACTAAATTCTCACATTACTTGCTAGACTCGACAAAGCGTTATCAAACT
ACTGTTCGATTAGGCCAAACTACAACAACAGGCGATGTTGAAGGCGATATTTTACAAGAACGTCCTATCCCGGTTCTTAG
CAAAGAGCTTATTGAGCAAACTTTAGAAAAGTTTAGGGGCGATATTCAACAAGTTCCACCAATGTACTCGGCATTAAAAA
AAGAAGGTAAGCCACTATATGAGCTGGCCAGAAAGGGTATAGAGATTGAGCGTGAAGCACGCCCGGTTACGATTTATGCC
TTAGAACTCATTGAATTTACTGAAAATAGTATCACTTTAGATGTAACTTGTTCTAAAGGAACATATATTCGTGTGCTAGG
TGAAGATATTGGTGAGGCACTTGGTTGTGGTGGTCATTTAACCATGCTTCACCGTATTCAAACTGGACATTTTGAACTCA
TCCCAAGTTATACCATTGAATATTTGGAAAGCTTAACGGAAGAGCAAAGAGAAGCTTTGCTACTGCCCGTATATGCTCCA
ATTGACCATTTCTTAAAAATTCAGGTTCCCGAAGGACGGGAAAAATATTTTTGTAACGGTCTAGAAAGCAATATTGATCA
TTCTGCAGAAGCAGAAGTGTTGGTTTTTTCGGGTGAACGCTGTCTAGGTTTAGCTGAAATTACCGATAAAAAACGGTTGG
TACCTAAACGTATCTTAAATTTGTAG

Upstream 100 bases:

>100_bases
TGCCATAAAATAAGTGAAAAGGCACCTAAATATTATGAAAATATCTTGCTGAATTTGCTAACATGTCGGCACTTTTCTCT
AGCTTTGTGTTCCAGTAAAA

Downstream 100 bases:

>100_bases
TAGATGTAGAGTATAAAACATGGGCATGGAATGGGATGTTATCCTTAGTTTGATCTTTTTTGCGTTTACTGCGGGAGCAA
TTGATGCTGCTGTTGGTGGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTTKNQKISRRHISGVFLLNKPLGLSSNAALQKVRWLYRAQKAGHTGALDPLATGLLPICLGEATKFSHYLLDSTKRYQT
TVRLGQTTTTGDVEGDILQERPIPVLSKELIEQTLEKFRGDIQQVPPMYSALKKEGKPLYELARKGIEIEREARPVTIYA
LELIEFTENSITLDVTCSKGTYIRVLGEDIGEALGCGGHLTMLHRIQTGHFELIPSYTIEYLESLTEEQREALLLPVYAP
IDHFLKIQVPEGREKYFCNGLESNIDHSAEAEVLVFSGERCLGLAEITDKKRLVPKRILNL

Sequences:

>Translated_301_residues
MTTKNQKISRRHISGVFLLNKPLGLSSNAALQKVRWLYRAQKAGHTGALDPLATGLLPICLGEATKFSHYLLDSTKRYQT
TVRLGQTTTTGDVEGDILQERPIPVLSKELIEQTLEKFRGDIQQVPPMYSALKKEGKPLYELARKGIEIEREARPVTIYA
LELIEFTENSITLDVTCSKGTYIRVLGEDIGEALGCGGHLTMLHRIQTGHFELIPSYTIEYLESLTEEQREALLLPVYAP
IDHFLKIQVPEGREKYFCNGLESNIDHSAEAEVLVFSGERCLGLAEITDKKRLVPKRILNL
>Mature_300_residues
TTKNQKISRRHISGVFLLNKPLGLSSNAALQKVRWLYRAQKAGHTGALDPLATGLLPICLGEATKFSHYLLDSTKRYQTT
VRLGQTTTTGDVEGDILQERPIPVLSKELIEQTLEKFRGDIQQVPPMYSALKKEGKPLYELARKGIEIEREARPVTIYAL
ELIEFTENSITLDVTCSKGTYIRVLGEDIGEALGCGGHLTMLHRIQTGHFELIPSYTIEYLESLTEEQREALLLPVYAPI
DHFLKIQVPEGREKYFCNGLESNIDHSAEAEVLVFSGERCLGLAEITDKKRLVPKRILNL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=218, Percent_Identity=38.9908256880734, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI4503337, Length=198, Percent_Identity=30.3030303030303, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI215599015, Length=198, Percent_Identity=30.3030303030303, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367200, Length=302, Percent_Identity=48.0132450331126, Blast_Score=280, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI17553978, Length=198, Percent_Identity=28.7878787878788, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=139, Percent_Identity=43.1654676258993, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323204, Length=199, Percent_Identity=32.6633165829146, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281364189, Length=207, Percent_Identity=29.4685990338164, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364187, Length=207, Percent_Identity=29.4685990338164, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364185, Length=207, Percent_Identity=29.4685990338164, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364183, Length=207, Percent_Identity=29.4685990338164, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62471759, Length=207, Percent_Identity=29.4685990338164, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17975520, Length=207, Percent_Identity=29.4685990338164, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33804; Mature: 33672

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKNQKISRRHISGVFLLNKPLGLSSNAALQKVRWLYRAQKAGHTGALDPLATGLLPIC
CCCCCCHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LGEATKFSHYLLDSTKRYQTTVRLGQTTTTGDVEGDILQERPIPVLSKELIEQTLEKFRG
HCCHHHHHHHHHCCCHHHHHEEEECCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHH
DIQQVPPMYSALKKEGKPLYELARKGIEIEREARPVTIYALELIEFTENSITLDVTCSKG
HHHHCCHHHHHHHHCCCHHHHHHHCCCCEECCCCCEEEEEEEEHHHCCCCEEEEEEECCC
TYIRVLGEDIGEALGCGGHLTMLHRIQTGHFELIPSYTIEYLESLTEEQREALLLPVYAP
CEEEEECHHHHHHHCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHEEEEEEECC
IDHFLKIQVPEGREKYFCNGLESNIDHSAEAEVLVFSGERCLGLAEITDKKRLVPKRILN
CCCEEEEECCCCCHHHHHCCHHCCCCCCCCCEEEEEECCCCEEHHHHCCHHHHHHHHHHC
L
C
>Mature Secondary Structure 
TTKNQKISRRHISGVFLLNKPLGLSSNAALQKVRWLYRAQKAGHTGALDPLATGLLPIC
CCCCCHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LGEATKFSHYLLDSTKRYQTTVRLGQTTTTGDVEGDILQERPIPVLSKELIEQTLEKFRG
HCCHHHHHHHHHCCCHHHHHEEEECCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHH
DIQQVPPMYSALKKEGKPLYELARKGIEIEREARPVTIYALELIEFTENSITLDVTCSKG
HHHHCCHHHHHHHHCCCHHHHHHHCCCCEECCCCCEEEEEEEEHHHCCCCEEEEEEECCC
TYIRVLGEDIGEALGCGGHLTMLHRIQTGHFELIPSYTIEYLESLTEEQREALLLPVYAP
CEEEEECHHHHHHHCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHEEEEEEECC
IDHFLKIQVPEGREKYFCNGLESNIDHSAEAEVLVFSGERCLGLAEITDKKRLVPKRILN
CCCEEEEECCCCCHHHHHCCHHCCCCCCCCCEEEEEECCCCEEHHHHCCHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA