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The map label for this gene is recG [H]

Identifier: 213158849

GI number: 213158849

Start: 3650304

End: 3652349

Strand: Direct

Name: recG [H]

Synonym: AB57_3544

Alternate gene names: 213158849

Gene position: 3650304-3652349 (Clockwise)

Preceding gene: 213158846

Following gene: 213158850

Centisome position: 90.12

GC content: 41.84

Gene sequence:

>2046_bases
ATGACTTCAGTCCATCAGTTACAAGGCGTTGGATCGGCTTCAGCAGCCCTACTCGAAAAACTAAATATTTTTACCACAGA
TGATTTGCTGTTTCATCTGCCCCGTGATTATGAAGATCGCAGTACCATTATTCCTATGAATCAATTGGTGGTTGGTCGTA
GTTATTTACTCGAGGGTGAAGTCAAATCTGTTGATTTCCCTCCAGGTAAACGTAAATCTATGGCCGCATTAATACAAGAT
GAATTTGGTAAGGTGACTTTACGCTTTTATCATATTTATAAAAATTTAACCGACAAAATAAAACCTGGTAACCGTTTACG
TATTTTTGGTGAAGTTCGCGTAGGTGCACGAGGGCTTGAGCTTTATCATCCAGAAATTCAACTTATTAATGAACATACAC
CACTACCAAAAACACAGCTCACTGCAATTTATCCAAGTACCGATGGCCTAACACAGGCTAAATTACGCGAATATGTTAAA
CAAGCGTTGAAGCATCATAGTGATGCTTTACCCGAGTTACTCCCTAAACAATATACAAATGGGTATGCACTCAAAGAAGC
TTTACATTACATTCATGAGCCGCCAGTTGATGCCAATATGATCCAATTGGCTCAAGGCTCTCATCCTGCGCAGCAACGTC
TTATTTTTGAAGAGCTGGTCGCACATCAAATTAGTCTTCTCACTCGACGTGCTTATATTCGTCAAATTGCTTCACCTGCT
TTTCCGAGTAGTAAAGTGCTTGCAAAAAAGCTGCTAGAAGCTTTACCTTTTCAAATGACCAACGCACAAAAACGTGTATC
AAAAGAAATTTTAAACGACTTAAAACAACATCAACCTATGCTGCGTTTGGTACAAGGTGATGTAGGGGCTGGAAAAACTT
TAGTTGCAGCGGTTGCAGCATGTCACGCATTAGAAGCAGATTGGCAAGTTGCGTTAATGGCACCCACCGAAATTTTAGCA
GAGCAGCATTATTTAAATTTTAAACGCTGGTTTGAACCTTTAGGTATTACGGTAGCTTGGTTGTCGGGTAAACAAAAAGG
GAAAGCGCGGGCACACGCTGAGCAACAAATTAAAGAAGGCCATGCTGAACTAATTGTGGGTACTCATGCCTTATTTCAAG
ATAACGTTGAATTTGCAAAACTAGGACTCGTGATTATTGATGAACAGCACCGATTCGGGGTCGATCAACGATTAGCCTTA
CGCAATAAAGGCGCCGAACAGCTCACCCCACATCAACTGGTCATGACTGCAACGCCAATTCCTAGAACACTGGCAATGAG
TGCTTATGGCGATTTAGATACATCAATTATTGATGAATTACCCCCAGGCCGAACTCCAATTCAGACCGTCACGATTCCGC
TAGATCGTCGTGAAGAAGTACTGCAACGAATTGCTTCAAACTGCCGAGAAGGCAAACAGGCCTACTGGGTATGTACTCTG
GTTGAACAGTCCGAAACTTTAGATGCTCAAGCCGCCGAAGCCACCTATCAAGAAATGAAAGAGCGCTTTCCTGAACTTAA
TATTGGTTTGGTTCATGGCAAAATGAAAGCCGATGAAAAACAGGCTGTCATGCAAGCATTTAAAAACAATGAACTACAGC
TGCTAATTGCCACAACTGTTATTGAGGTTGGGGTAGATGTACCTAACGCTTCGATTATGGTCATTGAGAATGCTGAACGA
TTAGGGCTTTCACAGCTACACCAATTACGAGGGCGTGTAGGACGAGGTGCAAAAGCCAGTTTTTGTGTTCTTCTCTATAA
ACCACCACTTTCACAAAATGGGCAAGAAAGACTTTCAATTTTAAGAGAAAGTAATGATGGCTTTGTTATTGCTGAAAAAG
ATCTTGAGCTAAGAGGCCCGGGTGAGTTATTAGGAACAAAACAAACAGGTGATATGGGTTTCCGTGTCGCACGTTTAGAG
CGAGATGATCATTTATTGAGTCAAGCACACTACGTTGCACAACAAGTTCTAAAAGATTACCCTGAGCAAGCTGATGCCTT
ATTGAAGCGCTGGCTTCCCGAAGCTCCTCGATATGCATATGTTTAA

Upstream 100 bases:

>100_bases
GTAATTTGATGTCCTTTCTCAAATAGTTGTTTAGCAACACGCTGAGATGTTTTACCATAACCGATAAATAATATATGCAT
AGAGAACTCGTTGAGGGGAC

Downstream 100 bases:

>100_bases
GTTTTTAAACCCACAGTATCTTTTTCAGTTGCTGTCCCCTTGCCTACTTTGCGAAATAGGAACACGAGAAAAATATTCTC
TTTGTAAAGAATGCTGGGAA

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MTSVHQLQGVGSASAALLEKLNIFTTDDLLFHLPRDYEDRSTIIPMNQLVVGRSYLLEGEVKSVDFPPGKRKSMAALIQD
EFGKVTLRFYHIYKNLTDKIKPGNRLRIFGEVRVGARGLELYHPEIQLINEHTPLPKTQLTAIYPSTDGLTQAKLREYVK
QALKHHSDALPELLPKQYTNGYALKEALHYIHEPPVDANMIQLAQGSHPAQQRLIFEELVAHQISLLTRRAYIRQIASPA
FPSSKVLAKKLLEALPFQMTNAQKRVSKEILNDLKQHQPMLRLVQGDVGAGKTLVAAVAACHALEADWQVALMAPTEILA
EQHYLNFKRWFEPLGITVAWLSGKQKGKARAHAEQQIKEGHAELIVGTHALFQDNVEFAKLGLVIIDEQHRFGVDQRLAL
RNKGAEQLTPHQLVMTATPIPRTLAMSAYGDLDTSIIDELPPGRTPIQTVTIPLDRREEVLQRIASNCREGKQAYWVCTL
VEQSETLDAQAAEATYQEMKERFPELNIGLVHGKMKADEKQAVMQAFKNNELQLLIATTVIEVGVDVPNASIMVIENAER
LGLSQLHQLRGRVGRGAKASFCVLLYKPPLSQNGQERLSILRESNDGFVIAEKDLELRGPGELLGTKQTGDMGFRVARLE
RDDHLLSQAHYVAQQVLKDYPEQADALLKRWLPEAPRYAYV

Sequences:

>Translated_681_residues
MTSVHQLQGVGSASAALLEKLNIFTTDDLLFHLPRDYEDRSTIIPMNQLVVGRSYLLEGEVKSVDFPPGKRKSMAALIQD
EFGKVTLRFYHIYKNLTDKIKPGNRLRIFGEVRVGARGLELYHPEIQLINEHTPLPKTQLTAIYPSTDGLTQAKLREYVK
QALKHHSDALPELLPKQYTNGYALKEALHYIHEPPVDANMIQLAQGSHPAQQRLIFEELVAHQISLLTRRAYIRQIASPA
FPSSKVLAKKLLEALPFQMTNAQKRVSKEILNDLKQHQPMLRLVQGDVGAGKTLVAAVAACHALEADWQVALMAPTEILA
EQHYLNFKRWFEPLGITVAWLSGKQKGKARAHAEQQIKEGHAELIVGTHALFQDNVEFAKLGLVIIDEQHRFGVDQRLAL
RNKGAEQLTPHQLVMTATPIPRTLAMSAYGDLDTSIIDELPPGRTPIQTVTIPLDRREEVLQRIASNCREGKQAYWVCTL
VEQSETLDAQAAEATYQEMKERFPELNIGLVHGKMKADEKQAVMQAFKNNELQLLIATTVIEVGVDVPNASIMVIENAER
LGLSQLHQLRGRVGRGAKASFCVLLYKPPLSQNGQERLSILRESNDGFVIAEKDLELRGPGELLGTKQTGDMGFRVARLE
RDDHLLSQAHYVAQQVLKDYPEQADALLKRWLPEAPRYAYV
>Mature_680_residues
TSVHQLQGVGSASAALLEKLNIFTTDDLLFHLPRDYEDRSTIIPMNQLVVGRSYLLEGEVKSVDFPPGKRKSMAALIQDE
FGKVTLRFYHIYKNLTDKIKPGNRLRIFGEVRVGARGLELYHPEIQLINEHTPLPKTQLTAIYPSTDGLTQAKLREYVKQ
ALKHHSDALPELLPKQYTNGYALKEALHYIHEPPVDANMIQLAQGSHPAQQRLIFEELVAHQISLLTRRAYIRQIASPAF
PSSKVLAKKLLEALPFQMTNAQKRVSKEILNDLKQHQPMLRLVQGDVGAGKTLVAAVAACHALEADWQVALMAPTEILAE
QHYLNFKRWFEPLGITVAWLSGKQKGKARAHAEQQIKEGHAELIVGTHALFQDNVEFAKLGLVIIDEQHRFGVDQRLALR
NKGAEQLTPHQLVMTATPIPRTLAMSAYGDLDTSIIDELPPGRTPIQTVTIPLDRREEVLQRIASNCREGKQAYWVCTLV
EQSETLDAQAAEATYQEMKERFPELNIGLVHGKMKADEKQAVMQAFKNNELQLLIATTVIEVGVDVPNASIMVIENAERL
GLSQLHQLRGRVGRGAKASFCVLLYKPPLSQNGQERLSILRESNDGFVIAEKDLELRGPGELLGTKQTGDMGFRVARLER
DDHLLSQAHYVAQQVLKDYPEQADALLKRWLPEAPRYAYV

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=678, Percent_Identity=55.4572271386431, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=401, Percent_Identity=37.9052369077307, Blast_Score=251, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76427; Mature: 76296

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVHQLQGVGSASAALLEKLNIFTTDDLLFHLPRDYEDRSTIIPMNQLVVGRSYLLEGE
CCCHHHHHCCCHHHHHHHHHHCCEEECHHHEECCCCCCCCCCCCCHHHHHHCHHHEECCC
VKSVDFPPGKRKSMAALIQDEFGKVTLRFYHIYKNLTDKIKPGNRLRIFGEVRVGARGLE
CCCCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCE
LYHPEIQLINEHTPLPKTQLTAIYPSTDGLTQAKLREYVKQALKHHSDALPELLPKQYTN
EECCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYALKEALHYIHEPPVDANMIQLAQGSHPAQQRLIFEELVAHQISLLTRRAYIRQIASPA
CHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FPSSKVLAKKLLEALPFQMTNAQKRVSKEILNDLKQHQPMLRLVQGDVGAGKTLVAAVAA
CCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHH
CHALEADWQVALMAPTEILAEQHYLNFKRWFEPLGITVAWLSGKQKGKARAHAEQQIKEG
HHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCC
HAELIVGTHALFQDNVEFAKLGLVIIDEQHRFGVDQRLALRNKGAEQLTPHQLVMTATPI
CCEEEEEHHHHHHCCCCEEHEEEEEEECCHHCCCHHHHHHHCCCCCCCCCCEEEEECCCC
PRTLAMSAYGDLDTSIIDELPPGRTPIQTVTIPLDRREEVLQRIASNCREGKQAYWVCTL
CHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEE
VEQSETLDAQAAEATYQEMKERFPELNIGLVHGKMKADEKQAVMQAFKNNELQLLIATTV
ECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEHH
IEVGVDVPNASIMVIENAERLGLSQLHQLRGRVGRGAKASFCVLLYKPPLSQNGQERLSI
HHHCCCCCCCEEEEEECHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHH
LRESNDGFVIAEKDLELRGPGELLGTKQTGDMGFRVARLERDDHLLSQAHYVAQQVLKDY
HHCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHC
PEQADALLKRWLPEAPRYAYV
CHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TSVHQLQGVGSASAALLEKLNIFTTDDLLFHLPRDYEDRSTIIPMNQLVVGRSYLLEGE
CCHHHHHCCCHHHHHHHHHHCCEEECHHHEECCCCCCCCCCCCCHHHHHHCHHHEECCC
VKSVDFPPGKRKSMAALIQDEFGKVTLRFYHIYKNLTDKIKPGNRLRIFGEVRVGARGLE
CCCCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCE
LYHPEIQLINEHTPLPKTQLTAIYPSTDGLTQAKLREYVKQALKHHSDALPELLPKQYTN
EECCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYALKEALHYIHEPPVDANMIQLAQGSHPAQQRLIFEELVAHQISLLTRRAYIRQIASPA
CHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FPSSKVLAKKLLEALPFQMTNAQKRVSKEILNDLKQHQPMLRLVQGDVGAGKTLVAAVAA
CCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHH
CHALEADWQVALMAPTEILAEQHYLNFKRWFEPLGITVAWLSGKQKGKARAHAEQQIKEG
HHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCC
HAELIVGTHALFQDNVEFAKLGLVIIDEQHRFGVDQRLALRNKGAEQLTPHQLVMTATPI
CCEEEEEHHHHHHCCCCEEHEEEEEEECCHHCCCHHHHHHHCCCCCCCCCCEEEEECCCC
PRTLAMSAYGDLDTSIIDELPPGRTPIQTVTIPLDRREEVLQRIASNCREGKQAYWVCTL
CHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEE
VEQSETLDAQAAEATYQEMKERFPELNIGLVHGKMKADEKQAVMQAFKNNELQLLIATTV
ECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEHH
IEVGVDVPNASIMVIENAERLGLSQLHQLRGRVGRGAKASFCVLLYKPPLSQNGQERLSI
HHHCCCCCCCEEEEEECHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHH
LRESNDGFVIAEKDLELRGPGELLGTKQTGDMGFRVARLERDDHLLSQAHYVAQQVLKDY
HHCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHC
PEQADALLKRWLPEAPRYAYV
CHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]