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The map label for this gene is hmp [H]

Identifier: 213158840

GI number: 213158840

Start: 3639983

End: 3640747

Strand: Direct

Name: hmp [H]

Synonym: AB57_3535

Alternate gene names: 213158840

Gene position: 3639983-3640747 (Clockwise)

Preceding gene: 213158839

Following gene: 213158841

Centisome position: 89.86

GC content: 39.22

Gene sequence:

>765_bases
ATGACTCCACAACAAATTGAACTCGTTAAATCTACTGTACCTGTATTGCGTGAACATGGTGTTACTCTAACTACATACTT
TTATAAGCGTATGTTGAATAACAACCCAGAACTAAAAAATGTTTTTAATCTTGATGATCAGACCAGCCTACGCCAACCTC
GTGCCCTTGCAGCAGCTGTACTCGCGTATGCTGAAAATATTGAGAATCCTACGGTGCTTGCCAAAGCGGTTGAACGCATT
ACCACTAAGCATGTCAGTTTAGATATTCAGCCAGATCAATATGCAATTGTGGGCGACAACTTGCTTCATTCAATTAGTGA
AGTTTTAAATGTTCCATTTGAATCAGAGCTCATTGAAGCGTGGAAACAAGCTTACTTACAGTTGGCAGATATCTTAATTG
GTGTTGAAAAACAAAAATATGAACAACTTGAAAGCTTGAAAGGTGGATGGGCTGGCTGGCGTTCATTTGAAATTACGCAA
ATTGACCCACTTGAGTCTGGCAAACGCTTTACTTTAAAGGCAACTGATCATGAAGATGTACTCACCAGCCCAGCAAATGC
TTTTATTTCTGTAAAGGTTCAAGTACCTAACCAACAGCTTGAACAACCAAAAGCATTTAAGTTCACGGAGGCTCAAGAGG
ACAATACTTATCATTTTGATGTTCAACCAGAAGAAGACCATACAGAGTTCTCTGTTTCAAACATTTTGCTTGAGCACTAT
AGAGTTGGTGATCAAGTACAAGTTTCAGCTCCTTTAACGCTGTAA

Upstream 100 bases:

>100_bases
TAAATTTTTTAAGGTTTTTATTTAAAAAGACTATTGCTACAACTATTTCAAAGATGTATTTTAAATACATCTTATATAGC
CAGATAATTAAGGTCTAGCC

Downstream 100 bases:

>100_bases
CTTAAAAAGCTCTGGCCTGAAAGTTTTATTCTTTAGACTTTCAGGCTAAGATGATCAACCTCTTAACCTTTAGTTTTTGG
TGATCATGCAACTTAATAAA

Product: flavohemoprotein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTPQQIELVKSTVPVLREHGVTLTTYFYKRMLNNNPELKNVFNLDDQTSLRQPRALAAAVLAYAENIENPTVLAKAVERI
TTKHVSLDIQPDQYAIVGDNLLHSISEVLNVPFESELIEAWKQAYLQLADILIGVEKQKYEQLESLKGGWAGWRSFEITQ
IDPLESGKRFTLKATDHEDVLTSPANAFISVKVQVPNQQLEQPKAFKFTEAQEDNTYHFDVQPEEDHTEFSVSNILLEHY
RVGDQVQVSAPLTL

Sequences:

>Translated_254_residues
MTPQQIELVKSTVPVLREHGVTLTTYFYKRMLNNNPELKNVFNLDDQTSLRQPRALAAAVLAYAENIENPTVLAKAVERI
TTKHVSLDIQPDQYAIVGDNLLHSISEVLNVPFESELIEAWKQAYLQLADILIGVEKQKYEQLESLKGGWAGWRSFEITQ
IDPLESGKRFTLKATDHEDVLTSPANAFISVKVQVPNQQLEQPKAFKFTEAQEDNTYHFDVQPEEDHTEFSVSNILLEHY
RVGDQVQVSAPLTL
>Mature_253_residues
TPQQIELVKSTVPVLREHGVTLTTYFYKRMLNNNPELKNVFNLDDQTSLRQPRALAAAVLAYAENIENPTVLAKAVERIT
TKHVSLDIQPDQYAIVGDNLLHSISEVLNVPFESELIEAWKQAYLQLADILIGVEKQKYEQLESLKGGWAGWRSFEITQI
DPLESGKRFTLKATDHEDVLTSPANAFISVKVQVPNQQLEQPKAFKFTEAQEDNTYHFDVQPEEDHTEFSVSNILLEHYR
VGDQVQVSAPLTL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1017

COG function: function code C; Hemoglobin-like flavoprotein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=252, Percent_Identity=37.6984126984127, Blast_Score=152, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321673, Length=255, Percent_Identity=38.0392156862745, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 28832; Mature: 28701

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00133 CARBOXYPEPT_ZN_2 ; PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQQIELVKSTVPVLREHGVTLTTYFYKRMLNNNPELKNVFNLDDQTSLRQPRALAAAV
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHEECCCCCHHHCCHHHHHHHH
LAYAENIENPTVLAKAVERITTKHVSLDIQPDQYAIVGDNLLHSISEVLNVPFESELIEA
HHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEECHHHHHHHHHHHCCCCHHHHHHH
WKQAYLQLADILIGVEKQKYEQLESLKGGWAGWRSFEITQIDPLESGKRFTLKATDHEDV
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEECCCHHH
LTSPANAFISVKVQVPNQQLEQPKAFKFTEAQEDNTYHFDVQPEEDHTEFSVSNILLEHY
HCCCCCEEEEEEEECCHHHHCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHH
RVGDQVQVSAPLTL
CCCCEEEECCCCCC
>Mature Secondary Structure 
TPQQIELVKSTVPVLREHGVTLTTYFYKRMLNNNPELKNVFNLDDQTSLRQPRALAAAV
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHEECCCCCHHHCCHHHHHHHH
LAYAENIENPTVLAKAVERITTKHVSLDIQPDQYAIVGDNLLHSISEVLNVPFESELIEA
HHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEECHHHHHHHHHHHCCCCHHHHHHH
WKQAYLQLADILIGVEKQKYEQLESLKGGWAGWRSFEITQIDPLESGKRFTLKATDHEDV
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEECCCHHH
LTSPANAFISVKVQVPNQQLEQPKAFKFTEAQEDNTYHFDVQPEEDHTEFSVSNILLEHY
HCCCCCEEEEEEEECCHHHHCCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHH
RVGDQVQVSAPLTL
CCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14521231; 11481432 [H]