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The map label for this gene is wrbA [H]

Identifier: 213158789

GI number: 213158789

Start: 3597620

End: 3598213

Strand: Reverse

Name: wrbA [H]

Synonym: AB57_3484

Alternate gene names: 213158789

Gene position: 3598213-3597620 (Counterclockwise)

Preceding gene: 213158791

Following gene: 213158785

Centisome position: 88.83

GC content: 40.07

Gene sequence:

>594_bases
ATGCAACCTTACATTCTTGTGCTTTATTACAGTAAATATGGTTCAACTAAAGAAATGGCGCACCTTATTGCGAATGGTGT
TGAATCGGCAGGTGTGAATGTAAAAATTCGCACAGTACCTAATATTGCAACAGTGGTAACAGAAGCTGAGCCAAGCATTC
CTGAAGAAGGTGATATTTATTGTACTTTAGAGGAACTGGCCAATTGTGCAGGCTTAGCGCTAGGGTCTCCAACTCGCTTT
GGTAATATGGCAAGCGAAATGAAATATTTTTGGGATCAGACTACAAGCCTTTGGTTAAATGGTGCTTTACATAATAAGCC
AGCATGCGTTTTTACTTCTTCTGGTTCAATGCATGGTGGGCAAGAAAGTACGTTATTAACGATGTTACCTCCATTATTTC
ATCATGGTATGATGATTTTAGGATTACCAAACTCTATTCCGGCTTTGTCGAATACTAAAACAGGTGGGACTCCCTATGGC
GCAAGCCACGTGAGTGGTCCAAGACATGACCAAAGTTTAAGCCAAGATGAAAAAGTTTTATGTGAAGCACAAGGGAAGCG
CTTAGGCGAAATCGTAAAAAAACTTCATTCTTAA

Upstream 100 bases:

>100_bases
CCTTAAAAATCAATCACCTCTAGAATTTCATAAAATTGGCTACGACCAAAAAAACAAAAAATACCGTATAGTGTTCTTTT
TAACAAAATTCGTGAAACCG

Downstream 100 bases:

>100_bases
ATTTATGGCAAATAAAAAAGAGCGGGTTTCCGCTCTTTTTTATTTAAACTATTTTATTCTTTTACAAAAGTTACTGTGCC
ATCAGCAAGTGATTGAACGC

Product: flavodoxin/nitric oxide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MQPYILVLYYSKYGSTKEMAHLIANGVESAGVNVKIRTVPNIATVVTEAEPSIPEEGDIYCTLEELANCAGLALGSPTRF
GNMASEMKYFWDQTTSLWLNGALHNKPACVFTSSGSMHGGQESTLLTMLPPLFHHGMMILGLPNSIPALSNTKTGGTPYG
ASHVSGPRHDQSLSQDEKVLCEAQGKRLGEIVKKLHS

Sequences:

>Translated_197_residues
MQPYILVLYYSKYGSTKEMAHLIANGVESAGVNVKIRTVPNIATVVTEAEPSIPEEGDIYCTLEELANCAGLALGSPTRF
GNMASEMKYFWDQTTSLWLNGALHNKPACVFTSSGSMHGGQESTLLTMLPPLFHHGMMILGLPNSIPALSNTKTGGTPYG
ASHVSGPRHDQSLSQDEKVLCEAQGKRLGEIVKKLHS
>Mature_197_residues
MQPYILVLYYSKYGSTKEMAHLIANGVESAGVNVKIRTVPNIATVVTEAEPSIPEEGDIYCTLEELANCAGLALGSPTRF
GNMASEMKYFWDQTTSLWLNGALHNKPACVFTSSGSMHGGQESTLLTMLPPLFHHGMMILGLPNSIPALSNTKTGGTPYG
ASHVSGPRHDQSLSQDEKVLCEAQGKRLGEIVKKLHS

Specific function: Seems To Enhance The Formation And/Or Stability Of Noncovalent Complexes Between The Trp Repressor Protein And Operator-Bearing DNA. However, Wrba Does Not Specifically Influence The Affinity Or Mode Of Binding Of Trpr To Its Operator. It Seems Possible T

COG id: COG0655

COG function: function code R; Multimeric flavodoxin WrbA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787239, Length=195, Percent_Identity=36.4102564102564, Blast_Score=120, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6319849, Length=199, Percent_Identity=36.6834170854271, Blast_Score=115, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320235, Length=198, Percent_Identity=34.8484848484849, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319526, Length=209, Percent_Identity=29.6650717703349, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008254
- InterPro:   IPR010089
- InterPro:   IPR005025 [H]

Pfam domain/function: PF03358 FMN_red [H]

EC number: NA

Molecular weight: Translated: 21235; Mature: 21235

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00201 FLAVODOXIN ; PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPYILVLYYSKYGSTKEMAHLIANGVESAGVNVKIRTVPNIATVVTEAEPSIPEEGDIY
CCCEEEEEEECCCCCHHHHHHHHHCCHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCEE
CTLEELANCAGLALGSPTRFGNMASEMKYFWDQTTSLWLNGALHNKPACVFTSSGSMHGG
EEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHEEEECCCCCCCEEEEECCCCCCCC
QESTLLTMLPPLFHHGMMILGLPNSIPALSNTKTGGTPYGASHVSGPRHDQSLSQDEKVL
CCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
CEAQGKRLGEIVKKLHS
HHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MQPYILVLYYSKYGSTKEMAHLIANGVESAGVNVKIRTVPNIATVVTEAEPSIPEEGDIY
CCCEEEEEEECCCCCHHHHHHHHHCCHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCEE
CTLEELANCAGLALGSPTRFGNMASEMKYFWDQTTSLWLNGALHNKPACVFTSSGSMHGG
EEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHEEEECCCCCCCEEEEECCCCCCCC
QESTLLTMLPPLFHHGMMILGLPNSIPALSNTKTGGTPYGASHVSGPRHDQSLSQDEKVL
CCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
CEAQGKRLGEIVKKLHS
HHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA