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The map label for this gene is glyA [H]

Identifier: 213158707

GI number: 213158707

Start: 2754766

End: 2756019

Strand: Reverse

Name: glyA [H]

Synonym: AB57_2665

Alternate gene names: 213158707

Gene position: 2756019-2754766 (Counterclockwise)

Preceding gene: 213158709

Following gene: 213158703

Centisome position: 68.04

GC content: 42.34

Gene sequence:

>1254_bases
ATGTTTGCCAATATTTCCATTTCTGAATTTGATCCAGAATTAGCTCAAGCAATTGCTTCTGAAGATGAGCGTCAAGAAGC
ACATATCGAGTTAATTGCATCTGAAAACTATTGCTCTCCTGCGGTAATGGAAGCGCAAGGATCAAAACTTACGAACAAAT
ACGCAGAAGGTTATCCTGGCAAACGCTATTATGGCGGTTGCGAATTTGTAGATGTTATTGAGCAAATGGCGATTGACCGT
GCTAAAGAACTTTTTGGTGCAGATTACGCAAACGTTCAACCACACGCTGGCTCACAAGCTAACTCTGCTGTTTATTTAGC
GCTTCTTAACCCGGGCGATACAGTTTTAGGTATGAGCTTGGCTCACGGTGGTCACTTGACTCACGGTGCAAAAGTAAGCT
TCTCTGGTAAAACGTATAACGCTGTTCAGTATGGTCTAAATGCTGAAACTGGTGAGATTGATTACGAAGAAGTTGAACGT
TTAGCATTGGAACACAAGCCACGTATGATCGTTGCTGGTTTCTCTGCTTATAGCCGTGTTGTAGATTGGCAACGTTTCCG
TGACATCGCGGACAAAGTTGGTGCTTACCTTTTTGTTGATATGGCTCACGTTGCTGGTCTTGTTGCTGCTGGTGTATATC
CAAACCCAGTTCAAATTGCTGACGTAACAACAACGACGACTCACAAAACACTTCGTGGTCCACGTTCTGGTTTAATCCTT
GCGAAAGCAAATGAAGAAATCGAGAAAAAACTTCAATCAGCTGTATTCCCTGGTAACCAAGGCGGTCCATTAATGCATGC
GATTGCTGCTAAAGCAATCTGCTTCAAAGAAGCAATGTCTGACGACTTTAAAGCTTACCAACAACAAGTTGTGAAAAATG
CTCAAGCAATGGCTGAAGTATTCATTGCTCGTGGTTATGATGTTGTTTCTGGTGGTACAGACAACCACTTATTCTTGTTA
TCTTTAATCAAACAAGACGTAACTGGTAAAGATGCAGACGCTTGGTTAGGTGCTGCTCACATTACTGTGAACAAAAACTC
AGTTCCAAATGACCCACGTTCTCCATTTGTGACTTCTGGTATCCGTATCGGTACTCCGGCAGTAACAACTCGTGGTTTTG
GTGAAGCTGAAGTTCGTGAACTTGCTGGTTGGATCGCTGACGTGATTGACAGCAAAGGTGACGAAAAAGTTATTGCTGAT
GTAAAAGCAAAAGTTGAAGCTGTTTGTGCAAAATTCCCTGTATATGCAAAATAA

Upstream 100 bases:

>100_bases
AAACTGAGCTAACTTCATTCTGTTTAATTATAAAGTTCATGCTACAATATGCCGCAATCTTAGCACGGCTTAAAAGCCCT
AATTTATAGGACCCCCGCTA

Downstream 100 bases:

>100_bases
TTTGTGTATATAAAAAAAGCGCCGTAAGGCGCTTTTTTTATTAACTACTCCAACCGCCCCCTAAAGCACGGATTAAATTG
ATGCTTGCAATGATTTGGCT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MFANISISEFDPELAQAIASEDERQEAHIELIASENYCSPAVMEAQGSKLTNKYAEGYPGKRYYGGCEFVDVIEQMAIDR
AKELFGADYANVQPHAGSQANSAVYLALLNPGDTVLGMSLAHGGHLTHGAKVSFSGKTYNAVQYGLNAETGEIDYEEVER
LALEHKPRMIVAGFSAYSRVVDWQRFRDIADKVGAYLFVDMAHVAGLVAAGVYPNPVQIADVTTTTTHKTLRGPRSGLIL
AKANEEIEKKLQSAVFPGNQGGPLMHAIAAKAICFKEAMSDDFKAYQQQVVKNAQAMAEVFIARGYDVVSGGTDNHLFLL
SLIKQDVTGKDADAWLGAAHITVNKNSVPNDPRSPFVTSGIRIGTPAVTTRGFGEAEVRELAGWIADVIDSKGDEKVIAD
VKAKVEAVCAKFPVYAK

Sequences:

>Translated_417_residues
MFANISISEFDPELAQAIASEDERQEAHIELIASENYCSPAVMEAQGSKLTNKYAEGYPGKRYYGGCEFVDVIEQMAIDR
AKELFGADYANVQPHAGSQANSAVYLALLNPGDTVLGMSLAHGGHLTHGAKVSFSGKTYNAVQYGLNAETGEIDYEEVER
LALEHKPRMIVAGFSAYSRVVDWQRFRDIADKVGAYLFVDMAHVAGLVAAGVYPNPVQIADVTTTTTHKTLRGPRSGLIL
AKANEEIEKKLQSAVFPGNQGGPLMHAIAAKAICFKEAMSDDFKAYQQQVVKNAQAMAEVFIARGYDVVSGGTDNHLFLL
SLIKQDVTGKDADAWLGAAHITVNKNSVPNDPRSPFVTSGIRIGTPAVTTRGFGEAEVRELAGWIADVIDSKGDEKVIAD
VKAKVEAVCAKFPVYAK
>Mature_417_residues
MFANISISEFDPELAQAIASEDERQEAHIELIASENYCSPAVMEAQGSKLTNKYAEGYPGKRYYGGCEFVDVIEQMAIDR
AKELFGADYANVQPHAGSQANSAVYLALLNPGDTVLGMSLAHGGHLTHGAKVSFSGKTYNAVQYGLNAETGEIDYEEVER
LALEHKPRMIVAGFSAYSRVVDWQRFRDIADKVGAYLFVDMAHVAGLVAAGVYPNPVQIADVTTTTTHKTLRGPRSGLIL
AKANEEIEKKLQSAVFPGNQGGPLMHAIAAKAICFKEAMSDDFKAYQQQVVKNAQAMAEVFIARGYDVVSGGTDNHLFLL
SLIKQDVTGKDADAWLGAAHITVNKNSVPNDPRSPFVTSGIRIGTPAVTTRGFGEAEVRELAGWIADVIDSKGDEKVIAD
VKAKVEAVCAKFPVYAK

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=453, Percent_Identity=44.5916114790287, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI261862352, Length=453, Percent_Identity=44.5916114790287, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI261862350, Length=453, Percent_Identity=44.5916114790287, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI261862348, Length=453, Percent_Identity=44.5916114790287, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI261862346, Length=454, Percent_Identity=43.6123348017621, Blast_Score=349, Evalue=2e-96,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=46.3840399002494, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=43.298969072165, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1788902, Length=413, Percent_Identity=72.6392251815981, Blast_Score=610, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=46.25, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=46.25, Blast_Score=358, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=46.8827930174564, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6323087, Length=426, Percent_Identity=43.6619718309859, Blast_Score=345, Evalue=9e-96,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=49.25, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=49.25, Blast_Score=382, Evalue=1e-106,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 44995; Mature: 44995

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFANISISEFDPELAQAIASEDERQEAHIELIASENYCSPAVMEAQGSKLTNKYAEGYPG
CCCCCCHHHCCHHHHHHHHCCHHHCCEEEEEEECCCCCCCHHHHCCCCHHHHHHHCCCCC
KRYYGGCEFVDVIEQMAIDRAKELFGADYANVQPHAGSQANSAVYLALLNPGDTVLGMSL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEE
AHGGHLTHGAKVSFSGKTYNAVQYGLNAETGEIDYEEVERLALEHKPRMIVAGFSAYSRV
CCCCCCCCCCEEEECCCCEEHEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWQRFRDIADKVGAYLFVDMAHVAGLVAAGVYPNPVQIADVTTTTTHKTLRGPRSGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHCCCCCCEEE
AKANEEIEKKLQSAVFPGNQGGPLMHAIAAKAICFKEAMSDDFKAYQQQVVKNAQAMAEV
EECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIARGYDVVSGGTDNHLFLLSLIKQDVTGKDADAWLGAAHITVNKNSVPNDPRSPFVTSG
HHHCCCEEECCCCCCHHHHHHHHHHCCCCCCCHHHEEEEEEEECCCCCCCCCCCCCEECC
IRIGTPAVTTRGFGEAEVRELAGWIADVIDSKGDEKVIADVKAKVEAVCAKFPVYAK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFANISISEFDPELAQAIASEDERQEAHIELIASENYCSPAVMEAQGSKLTNKYAEGYPG
CCCCCCHHHCCHHHHHHHHCCHHHCCEEEEEEECCCCCCCHHHHCCCCHHHHHHHCCCCC
KRYYGGCEFVDVIEQMAIDRAKELFGADYANVQPHAGSQANSAVYLALLNPGDTVLGMSL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEE
AHGGHLTHGAKVSFSGKTYNAVQYGLNAETGEIDYEEVERLALEHKPRMIVAGFSAYSRV
CCCCCCCCCCEEEECCCCEEHEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWQRFRDIADKVGAYLFVDMAHVAGLVAAGVYPNPVQIADVTTTTTHKTLRGPRSGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHCCCCCCEEE
AKANEEIEKKLQSAVFPGNQGGPLMHAIAAKAICFKEAMSDDFKAYQQQVVKNAQAMAEV
EECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIARGYDVVSGGTDNHLFLLSLIKQDVTGKDADAWLGAAHITVNKNSVPNDPRSPFVTSG
HHHCCCEEECCCCCCHHHHHHHHHHCCCCCCCHHHEEEEEEEECCCCCCCCCCCCCEECC
IRIGTPAVTTRGFGEAEVRELAGWIADVIDSKGDEKVIADVKAKVEAVCAKFPVYAK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA