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The map label for this gene is rpoH [H]

Identifier: 213158666

GI number: 213158666

Start: 2709825

End: 2710691

Strand: Direct

Name: rpoH [H]

Synonym: AB57_2620

Alternate gene names: 213158666

Gene position: 2709825-2710691 (Clockwise)

Preceding gene: 213158665

Following gene: 213158667

Centisome position: 66.9

GC content: 41.64

Gene sequence:

>867_bases
ATGAGTGACAGCAGCAATCAATTGATGCCCCTGTCATTGTCTGCGCCCGGTGTAAACCTTGGTGCATATATCAGTACTGT
CAATCAGATTCCTATTTTAACGGCTGAGCAAGAAAAAGAGCTTGCTGAGCGTTATTATTATGACCAAGATCTTGATGCCG
CAAAAATGCTGGTAATGTCGCATTTACGCTTCGTCGTTCACATTGCGCGCAGCTATGCAGGTTATGGCCTACCTCAAGGT
GACCTTATTCAAGAAGGTAACTTAGGCTTAATGAAAGCCGTAAAACGTTTTGACCCAAATATGGGCGTACGTCTTGTATC
TTTTGCCGTCCACTGGATTAAGGCAGAAATTCACGAATATGTTATTCGTAACTGGCGTATTGTAAAAATTGCAACAACCA
AAGCTCAGCGTAAACTTTTCTTCAATTTACGCAGCTTAAAAAAATCGAGTAAAAAGCTCACATTAGAAGAAGCGCAATCT
ATTGCAAACGACTTAAATGTTACTCCAGAACAAGTTTTGGAAATGGAAGGTCGTTTAACAGCATATGATGCTGCTTTTGA
AGCTCAAGGCGATGACGATGATGACACCCCGCACACTGCGCCAGTGTTATATCTTGAAGATAATCGTTATGACCCTGCTC
GTTTAGTAGAAAATGAGGATTGGGAAGAACAAAGTACATCTGCTTTGCATGACGCAATGAACCAGCTAGATGACCGTTCA
CGTAATATTTTGCAGCGCCGTTGGTTAGATGACGATAAATCTACCCTCCATGAGCTTGCTGCTGAATATAATGTTTCTGC
CGAACGTATTCGTCAGCTTGAAAAGAATGCGATGGAAAAAATTAAAGTCGCAATGTCTGCAAGCTAA

Upstream 100 bases:

>100_bases
AAAATTTTACATTTCTATACCCAATTTTCCTTAATTATGAAGCCGATTCAAATTAAGATGAACTTATTGGTTAATCCATC
CTATCCTATAAGGAGAACTC

Downstream 100 bases:

>100_bases
AGAAAATGAAAAAAATAAAAGGGCGATAAGCCCTTTTGTTTTATCTGGCTATGTTTAGCCAACCTATTCTATGGTAACGT
TGCCTTTCATTATTCAGTTG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMSHLRFVVHIARSYAGYGLPQG
DLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLEEAQS
IANDLNVTPEQVLEMEGRLTAYDAAFEAQGDDDDDTPHTAPVLYLEDNRYDPARLVENEDWEEQSTSALHDAMNQLDDRS
RNILQRRWLDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS

Sequences:

>Translated_288_residues
MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMSHLRFVVHIARSYAGYGLPQG
DLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLEEAQS
IANDLNVTPEQVLEMEGRLTAYDAAFEAQGDDDDDTPHTAPVLYLEDNRYDPARLVENEDWEEQSTSALHDAMNQLDDRS
RNILQRRWLDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS
>Mature_287_residues
SDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMSHLRFVVHIARSYAGYGLPQGD
LIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLEEAQSI
ANDLNVTPEQVLEMEGRLTAYDAAFEAQGDDDDDTPHTAPVLYLEDNRYDPARLVENEDWEEQSTSALHDAMNQLDDRSR
NILQRRWLDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=284, Percent_Identity=59.1549295774648, Blast_Score=330, Evalue=8e-92,
Organism=Escherichia coli, GI1789098, Length=266, Percent_Identity=28.1954887218045, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1789448, Length=259, Percent_Identity=28.1853281853282, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32832; Mature: 32701

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMS
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHH
HLRFVVHIARSYAGYGLPQGDLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEY
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLEEAQSIANDLNVTPEQVLEMEGRLT
HHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCEE
AYDAAFEAQGDDDDDTPHTAPVLYLEDNRYDPARLVENEDWEEQSTSALHDAMNQLDDRS
EEHHHEECCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RNILQRRWLDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS
HHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMS
CCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHH
HLRFVVHIARSYAGYGLPQGDLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEY
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VIRNWRIVKIATTKAQRKLFFNLRSLKKSSKKLTLEEAQSIANDLNVTPEQVLEMEGRLT
HHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCEE
AYDAAFEAQGDDDDDTPHTAPVLYLEDNRYDPARLVENEDWEEQSTSALHDAMNQLDDRS
EEHHHEECCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RNILQRRWLDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS
HHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7728657; 7698670; 10984043 [H]