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The map label for this gene is dctA [H]

Identifier: 213158578

GI number: 213158578

Start: 2626121

End: 2627419

Strand: Reverse

Name: dctA [H]

Synonym: AB57_2532

Alternate gene names: 213158578

Gene position: 2627419-2626121 (Counterclockwise)

Preceding gene: 213158579

Following gene: 213158566

Centisome position: 64.87

GC content: 39.57

Gene sequence:

>1299_bases
ATGGATTTCAATACCCTTGACCAAGAAACGGTACAGAAAAAACAAAAGTTTCATCAAATTTTGTATGTACAGGTCATCTT
TGCAATTATTTTGGGCATTTTAATTGGTCATTTTTACCCTGAACTTGGCGAGAGCTTAAAACCACTTGGTGATGCATTCA
TCAAAATCGTCAAAATGATTATTGCTCCCGTAATCTTTTTGACAGTGGTTACCGGTATTGCCAGCATGACGAACATGAGT
CGAGTTGGGCGAGTGACAGGTAAGGCAATGCTATATTTCCTTACTTTCTCTAGCCTTGCGCTTGTGGTTGGTATGATTGT
GGCTAATATCATTCAACCTGGTAAAGGCTTAAATATTGACCCGGCAACCTTAGTCAACGATAAAGTAAATAGTTACGTTG
AAAAGGCTCATGCTACAAATATCACTGACTTTTTCATGAATATTATCCCGCAAACATTAGTGAGTCCGCTTGCTGGTGGT
GAAATTTTGCAGGTTCTATTTGTAGCAGTCCTATTTGGTATTTCTTTAGCTGCCTTGGGTGACCGTGCACGTCCCATTAC
TGATTTTTTAAATAACCTCACGGCTCCGGTTTTCTATTTGGTTGGCATGTTAATGAAACTTGCACCAATTGGAGCTTTTG
GTGCTATGGCATTTACTATCGGTGCGTATGGAATCGAATCAATCGGTAATTTATTACTATTGATTATGACTTTCTATATC
ACGGCAGTGCTGTTCGTATTAATCATTTTAGGGGCTGTAGCACGCTACAATGGCTTTTCAATTATTGATTTAATTCGTTA
CATCAAAGACGAACTCTGGTTAGTTTTAGGTACAAGCTCTTCTGAAGCTGCTTTACCATCTTTAATGGATAAAATGCAAA
AAGCAGGTTGTGAAAAATCTGTAGTAGGTCTAGTCATTCCGACAGGTTACTCATTTAACCTTGATGGAACTAATATCTAT
ATGACTATGGCGGCTTTATTTATTGCACAAGCATGTAATGTTGATTTATCAATTGGTGAGCAGGTTGCATTGCTTTTAGT
GGCAATGGTGAGTTCTAAAGGTGCGGCAGGGGTAACGGGCGCAGGCTTTATTACACTCGCAGCTACTTTATCGGTAGTAC
CTGCTGTACCAGTGGCAGGTATGGCGCTTATTTTAGGTATTGACCGTTTTATGTCGGAGTGTCGTGCATTAACCAATTTA
ATTGGTAATGCATGTGCAACCATTGTGGTTGCCCGTTGGGACAAAGCTCTGGACAAGGATGTTCTAGATAAAGCATTACG
CAAATCGAAAACGAATTAA

Upstream 100 bases:

>100_bases
TGTTTTTTATTCTTTACAATGGCTCCACTTCATTTAAAGCAGCTTGTGGTGCACGCTTTATATTTTATTTTTTTATCCCC
ATCGAAAAGGTATTTAGATA

Downstream 100 bases:

>100_bases
TTATTTAATTTTTTAATTCGAAATAAAAAACCTGTAGCAAGTGGCAAATCGGGAGAGGTGCTGTGCTACAGGTTTTATTA
TTTAATGATCAAAAAAATAT

Product: C4-dicarboxylate transport protein

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MDFNTLDQETVQKKQKFHQILYVQVIFAIILGILIGHFYPELGESLKPLGDAFIKIVKMIIAPVIFLTVVTGIASMTNMS
RVGRVTGKAMLYFLTFSSLALVVGMIVANIIQPGKGLNIDPATLVNDKVNSYVEKAHATNITDFFMNIIPQTLVSPLAGG
EILQVLFVAVLFGISLAALGDRARPITDFLNNLTAPVFYLVGMLMKLAPIGAFGAMAFTIGAYGIESIGNLLLLIMTFYI
TAVLFVLIILGAVARYNGFSIIDLIRYIKDELWLVLGTSSSEAALPSLMDKMQKAGCEKSVVGLVIPTGYSFNLDGTNIY
MTMAALFIAQACNVDLSIGEQVALLLVAMVSSKGAAGVTGAGFITLAATLSVVPAVPVAGMALILGIDRFMSECRALTNL
IGNACATIVVARWDKALDKDVLDKALRKSKTN

Sequences:

>Translated_432_residues
MDFNTLDQETVQKKQKFHQILYVQVIFAIILGILIGHFYPELGESLKPLGDAFIKIVKMIIAPVIFLTVVTGIASMTNMS
RVGRVTGKAMLYFLTFSSLALVVGMIVANIIQPGKGLNIDPATLVNDKVNSYVEKAHATNITDFFMNIIPQTLVSPLAGG
EILQVLFVAVLFGISLAALGDRARPITDFLNNLTAPVFYLVGMLMKLAPIGAFGAMAFTIGAYGIESIGNLLLLIMTFYI
TAVLFVLIILGAVARYNGFSIIDLIRYIKDELWLVLGTSSSEAALPSLMDKMQKAGCEKSVVGLVIPTGYSFNLDGTNIY
MTMAALFIAQACNVDLSIGEQVALLLVAMVSSKGAAGVTGAGFITLAATLSVVPAVPVAGMALILGIDRFMSECRALTNL
IGNACATIVVARWDKALDKDVLDKALRKSKTN
>Mature_432_residues
MDFNTLDQETVQKKQKFHQILYVQVIFAIILGILIGHFYPELGESLKPLGDAFIKIVKMIIAPVIFLTVVTGIASMTNMS
RVGRVTGKAMLYFLTFSSLALVVGMIVANIIQPGKGLNIDPATLVNDKVNSYVEKAHATNITDFFMNIIPQTLVSPLAGG
EILQVLFVAVLFGISLAALGDRARPITDFLNNLTAPVFYLVGMLMKLAPIGAFGAMAFTIGAYGIESIGNLLLLIMTFYI
TAVLFVLIILGAVARYNGFSIIDLIRYIKDELWLVLGTSSSEAALPSLMDKMQKAGCEKSVVGLVIPTGYSFNLDGTNIY
MTMAALFIAQACNVDLSIGEQVALLLVAMVSSKGAAGVTGAGFITLAATLSVVPAVPVAGMALILGIDRFMSECRALTNL
IGNACATIVVARWDKALDKDVLDKALRKSKTN

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=442, Percent_Identity=24.4343891402715, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI21314632, Length=419, Percent_Identity=26.2529832935561, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI40254478, Length=473, Percent_Identity=26.6384778012685, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194239697, Length=475, Percent_Identity=24.6315789473684, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI169790839, Length=460, Percent_Identity=25.2173913043478, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI66773030, Length=403, Percent_Identity=24.317617866005, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI5032093, Length=294, Percent_Identity=30.2721088435374, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI223468566, Length=253, Percent_Identity=31.2252964426877, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI223468564, Length=250, Percent_Identity=31.2, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI301601644, Length=166, Percent_Identity=34.9397590361446, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI262359914, Length=385, Percent_Identity=24.6753246753247, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789947, Length=415, Percent_Identity=58.5542168674699, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1790514, Length=430, Percent_Identity=37.4418604651163, Blast_Score=297, Evalue=8e-82,
Organism=Escherichia coli, GI1788024, Length=391, Percent_Identity=23.2736572890026, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71996953, Length=436, Percent_Identity=27.5229357798165, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17537407, Length=444, Percent_Identity=25.4504504504505, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983099, Length=472, Percent_Identity=23.728813559322, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983106, Length=472, Percent_Identity=23.728813559322, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI193206505, Length=455, Percent_Identity=25.7142857142857, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=426, Percent_Identity=25.1173708920188, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=428, Percent_Identity=24.0654205607477, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583025, Length=409, Percent_Identity=24.4498777506112, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17137668, Length=409, Percent_Identity=24.4498777506112, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24583023, Length=409, Percent_Identity=24.4498777506112, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI281360481, Length=454, Percent_Identity=23.568281938326, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281360483, Length=454, Percent_Identity=23.568281938326, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137666, Length=454, Percent_Identity=23.568281938326, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46262; Mature: 46262

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA