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The map label for this gene is gloB [H]

Identifier: 213158576

GI number: 213158576

Start: 2624586

End: 2625317

Strand: Direct

Name: gloB [H]

Synonym: AB57_2530

Alternate gene names: 213158576

Gene position: 2624586-2625317 (Clockwise)

Preceding gene: 213158575

Following gene: 213158577

Centisome position: 64.8

GC content: 39.48

Gene sequence:

>732_bases
ATGCGTTATAGAATTCATTATATTGATGTGCAAAATGCCCTACAAAACTATATCTGGATTTTAGAAGATACTGAGACTCA
TGAAGCTGTTGCAGTTGATCCAACAGAGGCTGAGTTAGTCACCCACTTTTGCCAAGAACATCAATTAACTCTAAAACAGA
TTTGGCTTACCCATTGGCATAAAGACCATATCGGTGGTGTTGCTGATTTAACGGCTAACACGCCTATGGCGGTATATGGC
CCAAGAGATGAACTAAGTAAAATCCCTGGCATTACTCATCCCTTACAACACGATGATCATCTAAAATTTAATGATTTAAA
AGTAGAGATTATTGCAACACCAGGCCATACCTTAGGTCATATTGTATATTTTATTGAAGAGCTTAATGCCCTCTTTTGTG
GAGATACCTTATTTGCAATGGGATGCGGGCGACTATTTGAAGGTACAGCAGAACAGATGTACCACTCACTAAATCGTCTT
GCTGCACTACCCACTGCAACCAAAGTGTATTGCACACATGAATATACACTTTCTAATGCGGAATTTGCCTTGAGTGTAGA
GCCTGAAAACCACGCACTGCAAGAACGTGCTGAACAAGTCCGTATGCTTCGTCAAGAAGGTAAAATTACCTTGCCAAGTA
GTATTGAACTTGAACTAGCAACAAATCCCTTTTTACGTGCTGAAAGTGTAGATGAGTTCGCTCACTTACGTAGCTTAAAA
GACAATTTTTAA

Upstream 100 bases:

>100_bases
TTATAGAGCATTTTATTAGACAGGTTACTCAAGGTAATTTCTGTTATTATCAAAATTAGCCCGTAGATACTGCTTTTGGA
TTTAGTTTCAGGATAAAAAA

Downstream 100 bases:

>100_bases
AATTATAAGCTTTCAACCAAGATACAGTTTAATTTTAAGAACTGTATCTTTTTGAATAAAGCATGTTGCATGAAATTATT
TTTTACATTTATTGATTTTA

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRYRIHYIDVQNALQNYIWILEDTETHEAVAVDPTEAELVTHFCQEHQLTLKQIWLTHWHKDHIGGVADLTANTPMAVYG
PRDELSKIPGITHPLQHDDHLKFNDLKVEIIATPGHTLGHIVYFIEELNALFCGDTLFAMGCGRLFEGTAEQMYHSLNRL
AALPTATKVYCTHEYTLSNAEFALSVEPENHALQERAEQVRMLRQEGKITLPSSIELELATNPFLRAESVDEFAHLRSLK
DNF

Sequences:

>Translated_243_residues
MRYRIHYIDVQNALQNYIWILEDTETHEAVAVDPTEAELVTHFCQEHQLTLKQIWLTHWHKDHIGGVADLTANTPMAVYG
PRDELSKIPGITHPLQHDDHLKFNDLKVEIIATPGHTLGHIVYFIEELNALFCGDTLFAMGCGRLFEGTAEQMYHSLNRL
AALPTATKVYCTHEYTLSNAEFALSVEPENHALQERAEQVRMLRQEGKITLPSSIELELATNPFLRAESVDEFAHLRSLK
DNF
>Mature_243_residues
MRYRIHYIDVQNALQNYIWILEDTETHEAVAVDPTEAELVTHFCQEHQLTLKQIWLTHWHKDHIGGVADLTANTPMAVYG
PRDELSKIPGITHPLQHDDHLKFNDLKVEIIATPGHTLGHIVYFIEELNALFCGDTLFAMGCGRLFEGTAEQMYHSLNRL
AALPTATKVYCTHEYTLSNAEFALSVEPENHALQERAEQVRMLRQEGKITLPSSIELELATNPFLRAESVDEFAHLRSLK
DNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=227, Percent_Identity=40.0881057268723, Blast_Score=160, Evalue=8e-40,
Organism=Homo sapiens, GI94538320, Length=227, Percent_Identity=40.0881057268723, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI116642887, Length=218, Percent_Identity=42.2018348623853, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI21703352, Length=218, Percent_Identity=42.2018348623853, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI14150041, Length=226, Percent_Identity=39.3805309734513, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI46361987, Length=201, Percent_Identity=40.2985074626866, Blast_Score=130, Evalue=8e-31,
Organism=Homo sapiens, GI41327741, Length=226, Percent_Identity=28.7610619469027, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1786406, Length=245, Percent_Identity=44.4897959183673, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1787158, Length=163, Percent_Identity=31.9018404907975, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536925, Length=250, Percent_Identity=34, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6320478, Length=237, Percent_Identity=32.9113924050633, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324614, Length=233, Percent_Identity=27.4678111587983, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356335, Length=258, Percent_Identity=42.6356589147287, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24667703, Length=258, Percent_Identity=42.6356589147287, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24667711, Length=258, Percent_Identity=42.6356589147287, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI221330176, Length=213, Percent_Identity=26.7605633802817, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 27742; Mature: 27742

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYRIHYIDVQNALQNYIWILEDTETHEAVAVDPTEAELVTHFCQEHQLTLKQIWLTHWH
CCEEEEEEEHHHHHHHEEEEEECCCCCCEEEECCCHHHHHHHHHHHHCHHHHHHHHHHCC
KDHIGGVADLTANTPMAVYGPRDELSKIPGITHPLQHDDHLKFNDLKVEIIATPGHTLGH
HHHCCCEEEECCCCCEEEECCHHHHHHCCCCCCCCCCCCCCEECCEEEEEEECCCCHHHH
IVYFIEELNALFCGDTLFAMGCGRLFEGTAEQMYHSLNRLAALPTATKVYCTHEYTLSNA
HHHHHHHHHHHHCCCHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCC
EFALSVEPENHALQERAEQVRMLRQEGKITLPSSIELELATNPFLRAESVDEFAHLRSLK
EEEEEECCCHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCHHHHHHHHHHH
DNF
CCC
>Mature Secondary Structure
MRYRIHYIDVQNALQNYIWILEDTETHEAVAVDPTEAELVTHFCQEHQLTLKQIWLTHWH
CCEEEEEEEHHHHHHHEEEEEECCCCCCEEEECCCHHHHHHHHHHHHCHHHHHHHHHHCC
KDHIGGVADLTANTPMAVYGPRDELSKIPGITHPLQHDDHLKFNDLKVEIIATPGHTLGH
HHHCCCEEEECCCCCEEEECCHHHHHHCCCCCCCCCCCCCCEECCEEEEEEECCCCHHHH
IVYFIEELNALFCGDTLFAMGCGRLFEGTAEQMYHSLNRLAALPTATKVYCTHEYTLSNA
HHHHHHHHHHHHCCCHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCC
EFALSVEPENHALQERAEQVRMLRQEGKITLPSSIELELATNPFLRAESVDEFAHLRSLK
EEEEEECCCHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCHHHHHHHHHHH
DNF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA