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The map label for this gene is ppsA [H]

Identifier: 213158549

GI number: 213158549

Start: 2595742

End: 2598237

Strand: Direct

Name: ppsA [H]

Synonym: AB57_2501

Alternate gene names: 213158549

Gene position: 2595742-2598237 (Clockwise)

Preceding gene: 213158542

Following gene: 213158550

Centisome position: 64.08

GC content: 42.07

Gene sequence:

>2496_bases
TTGGAAGCGCGCGTAATCGGTCTAGAAAAATTAGGGAAACACGATGTCGAACTCGTAGGTGGGAAAAACTCATCTTTGGG
TGAAATGATCAGCCATTTATCAAATGCTGGTGTATCGGTACCTGGTGGTTTTGCAACAACTGCTGATGCCTATCGTGAGT
TCTTAGACCAAAGCGGTCTTAACGCTCGAATTCAAGCAGAACTTGCAGAGCTTAACGTTGACGATGTAAATGCCTTAGCA
GAAACTGGCGCTAAAATTCGTCAATGGATTGTAGAGACTCCACTTACACCTGGCTTAGAACAAGAAATTCGTGAAGCATT
TGCTGCACTTTCAAATGGTAACCCAGATATTGCTGTTGCTGTTCGTTCGTCTGCTACTGCAGAAGACTTACCGGATGCAT
CTTTCGCCGGTCAGCAAGAAACTTTCTTGAATATTCGCGGTATTGATAACGTATTAATTGCTGTAAAAGAAGTATTTGCA
TCATTATTTAATGACCGTGCTATTGCATATCGCGTTCACCAAGGTTTTGAACACAGTCTAGTTGCGCTTTCTGCTGGTGT
TCAACGTATGGTTCGCTCTGAAACTGGCGCTGCAGGCGTAATGTTTACCCTTGATACTGAGTCTGGCTTCCGTGATGTTG
TATTTATTACTGCATCTTACGGTTTAGGTGAAATGGTTGTACAAGGTGCCGTTAACCCGGATGAATTCTATCTTTCTAAA
CCATTATTAAATGCAGGTAAACACGCGGTATTACGCCGTAACCTTGGTTCTAAGCACCAGAAAATGATTTATGGTGAAGA
AGGTTCAGCGGGTAAATCTGTTGTAGTTGTAGACGTTGAAAAACAAGAACGTCAACAATTCGCGTTAAATGATCACGAGT
TACAAGAGTTAGCGAAACAAGCGCTTATTATCGAAAAGCACTATGGCGCTCCAATGGATATCGAGTGGGCAAAAGATGGT
GACGATGGTCAAATCTATATTGTTCAAGCTCGTCCTGAAACTGTAAAGAGCCGTCAAAATGTCGGCACAATGGAACGCTA
CCTACTCAAGCAAAAAGGTACTGTACTTTGTGAAGGCCGTTCAATCGGTCAACGTATTGGTTCTGGTAAAGTCCGTATTG
TTAACTCAATTAAAGAAATGGACAAAGTACAAGACGGTGACGTACTTGTATCAGACATGACTGACCCAGATTGGGAACCA
GTAATGAAACGTGCTGCTGCCATCATTACTAACCGTGGTGGCCGTACTTGTCACGCTGCAATTATTGCCCGTGAACTTGG
CGTACCAGCAATCGTAGGTTGTGGTAACGCAACTGAAGTATTGACTGATGGTCAAGAAGTTACTGTTTCTTGTGCTGAAG
GCGACACTGGTTTCATCTACGAAGGCGCGTTAGATTTTGAAGTTCAACGTAACTCTATCGAATCTATGCCTAAACTTTCA
TTCAAAATCATGATGAACGTAGGTAACCCTGACCGTGCCTTTGACTTTGCTCAGATTCCAAACGAAGGTATTGGACTTGC
TCGTCTAGAGTTCATTATTAACCGTATGATCGGTGTTCACCCTAAAGCTCTTCTGAACATTGAAAGTTTGCCTCGCGAAA
CTCGTGCAGCGGTAATGACCCGTACAGCTGGTTATGCATCACCTGTAGAATTCTATGTTGAAAAACTAGTTGAAGGTATT
GCTACACTTGCAGCAGCATTTGCTGATAAACCAGTTATTGTTCGTATGTCTGACTTTAAGTCAAATGAATATGCGAATCT
TATTGGCGGTAAGTTATACGAGCCAGAAGAAGAAAACCCAATGCTTGGCTTCCGTGGCGCAAGCCGTTATGTTTCGGACA
ATTTCCGTGACTGTTTCGAACTTGAATGCCGCGCACTTAAAAAAGTTCGTGATGAAATGGGTTTAACTAACATCCAAATC
ATGATCCCATTCGTACGTACAGTGTCTGAAGCGAAACGCGTTATTGAGTTATTAGCTCAAAATGGCTTGAAGCGTGGTGA
GAATGGCTTAAAAGTCATCATGATGTGTGAATTACCAACTAATGCATTGTTAGCTGAACAATTCCTTGAACACTTCGATG
GCTTCTCTATCGGTTCTAACGACTTAACTCAGTTAACACTTGGTCTTGACCGTGACTCTGGTATTGTTTCTCACTTGTTC
GATGAGCGTGATGCTGCTGTAAAAGCTCTCCTTTCTATGGCAATTCATGCTTGTCGTAAAGCGGGTAAATATGTCGGTAT
CTGTGGTCAAGGACCATCAGACCACCCTGACCTTGCAAAATGGTTAATGGAGCAAGGCATTGAATCAGTATCTCTTAACC
CTGACTCGGTTTTAGACACATGGTTCTTCCTTGCTGAAAGAAAAAATCAAGCAAATCTAATTTGTTATAAAAAAAGACCC
ATCCGTGGGTCTTTTTTCATCTTTAGAATAGAGAATTTTTACTTATGCAAATTTACTTGGCACGTAATAATCAACAAGCT
GGGCCTTATACCTTAG

Upstream 100 bases:

>100_bases
TCTTACATGACTGTCGCTTAGTAAAATTCTCAACTAAGCACTTTATTTTTTAATTTCATGCCAAGATAGACATTAATACT
GCAAAAGTGGAGTGAAAACT

Downstream 100 bases:

>100_bases
AACAACTAAATCAAATGTTGGCAAGTCAACAAGTTTTGCTTACTGATTTAGCTTGGCATGAAGGCATGACAGAATGGAAA
GCTTTAGGAGAATTAACCCA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 831; Mature: 831

Protein sequence:

>831_residues
MEARVIGLEKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTADAYREFLDQSGLNARIQAELAELNVDDVNALA
ETGAKIRQWIVETPLTPGLEQEIREAFAALSNGNPDIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAVKEVFA
SLFNDRAIAYRVHQGFEHSLVALSAGVQRMVRSETGAAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSK
PLLNAGKHAVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQERQQFALNDHELQELAKQALIIEKHYGAPMDIEWAKDG
DDGQIYIVQARPETVKSRQNVGTMERYLLKQKGTVLCEGRSIGQRIGSGKVRIVNSIKEMDKVQDGDVLVSDMTDPDWEP
VMKRAAAIITNRGGRTCHAAIIARELGVPAIVGCGNATEVLTDGQEVTVSCAEGDTGFIYEGALDFEVQRNSIESMPKLS
FKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIESLPRETRAAVMTRTAGYASPVEFYVEKLVEGI
ATLAAAFADKPVIVRMSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYVSDNFRDCFELECRALKKVRDEMGLTNIQI
MIPFVRTVSEAKRVIELLAQNGLKRGENGLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLF
DERDAAVKALLSMAIHACRKAGKYVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDTWFFLAERKNQANLICYKKRP
IRGSFFIFRIENFYLCKFTWHVIINKLGLIP

Sequences:

>Translated_831_residues
MEARVIGLEKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTADAYREFLDQSGLNARIQAELAELNVDDVNALA
ETGAKIRQWIVETPLTPGLEQEIREAFAALSNGNPDIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAVKEVFA
SLFNDRAIAYRVHQGFEHSLVALSAGVQRMVRSETGAAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSK
PLLNAGKHAVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQERQQFALNDHELQELAKQALIIEKHYGAPMDIEWAKDG
DDGQIYIVQARPETVKSRQNVGTMERYLLKQKGTVLCEGRSIGQRIGSGKVRIVNSIKEMDKVQDGDVLVSDMTDPDWEP
VMKRAAAIITNRGGRTCHAAIIARELGVPAIVGCGNATEVLTDGQEVTVSCAEGDTGFIYEGALDFEVQRNSIESMPKLS
FKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIESLPRETRAAVMTRTAGYASPVEFYVEKLVEGI
ATLAAAFADKPVIVRMSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYVSDNFRDCFELECRALKKVRDEMGLTNIQI
MIPFVRTVSEAKRVIELLAQNGLKRGENGLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLF
DERDAAVKALLSMAIHACRKAGKYVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDTWFFLAERKNQANLICYKKRP
IRGSFFIFRIENFYLCKFTWHVIINKLGLIP
>Mature_831_residues
MEARVIGLEKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTADAYREFLDQSGLNARIQAELAELNVDDVNALA
ETGAKIRQWIVETPLTPGLEQEIREAFAALSNGNPDIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAVKEVFA
SLFNDRAIAYRVHQGFEHSLVALSAGVQRMVRSETGAAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSK
PLLNAGKHAVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQERQQFALNDHELQELAKQALIIEKHYGAPMDIEWAKDG
DDGQIYIVQARPETVKSRQNVGTMERYLLKQKGTVLCEGRSIGQRIGSGKVRIVNSIKEMDKVQDGDVLVSDMTDPDWEP
VMKRAAAIITNRGGRTCHAAIIARELGVPAIVGCGNATEVLTDGQEVTVSCAEGDTGFIYEGALDFEVQRNSIESMPKLS
FKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIESLPRETRAAVMTRTAGYASPVEFYVEKLVEGI
ATLAAAFADKPVIVRMSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYVSDNFRDCFELECRALKKVRDEMGLTNIQI
MIPFVRTVSEAKRVIELLAQNGLKRGENGLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLF
DERDAAVKALLSMAIHACRKAGKYVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDTWFFLAERKNQANLICYKKRP
IRGSFFIFRIENFYLCKFTWHVIINKLGLIP

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=784, Percent_Identity=67.4744897959184, Blast_Score=1126, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=448, Percent_Identity=26.1160714285714, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1788726, Length=382, Percent_Identity=25.130890052356, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI48994992, Length=372, Percent_Identity=25, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1789193, Length=410, Percent_Identity=23.9024390243902, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17564524, Length=329, Percent_Identity=26.7477203647416, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 91408; Mature: 91408

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEARVIGLEKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTADAYREFLDQSGL
CCCEEECHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
NARIQAELAELNVDDVNALAETGAKIRQWIVETPLTPGLEQEIREAFAALSNGNPDIAVA
CEEEEHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEE
VRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAVKEVFASLFNDRAIAYRVHQGFEHSL
EECCCCHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
VALSAGVQRMVRSETGAAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSK
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCHHHHHHCCCCHHHHHHCC
PLLNAGKHAVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQERQQFALNDHELQELAKQ
HHHHCCHHHHHHHHCCCCHHEEEECCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHH
ALIIEKHYGAPMDIEWAKDGDDGQIYIVQARPETVKSRQNVGTMERYLLKQKGTVLCEGR
HHHHHHCCCCCCEEEECCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHCCCCEEECCC
SIGQRIGSGKVRIVNSIKEMDKVQDGDVLVSDMTDPDWEPVMKRAAAIITNRGGRTCHAA
HHHHHCCCCCEEEHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHH
IIARELGVPAIVGCGNATEVLTDGQEVTVSCAEGDTGFIYEGALDFEVQRNSIESMPKLS
HHHHHCCCCEEEECCCCHHHHCCCCEEEEEEECCCCCEEEECCEEEEECHHHHHCCCCCE
FKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIESLPRETRAAVMT
EEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCHHHEEHHHCCCHHHHHHHH
RTAGYASPVEFYVEKLVEGIATLAAAFADKPVIVRMSDFKSNEYANLIGGKLYEPEEENP
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCC
MLGFRGASRYVSDNFRDCFELECRALKKVRDEMGLTNIQIMIPFVRTVSEAKRVIELLAQ
CEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHH
NGLKRGENGLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLF
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHH
DERDAAVKALLSMAIHACRKAGKYVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDT
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCEECCCCHHHHHH
WFFLAERKNQANLICYKKRPIRGSFFIFRIENFYLCKFTWHVIINKLGLIP
HHHHHHCCCCCCEEEEECCCCCCCEEEEEECCEEEEEEHHHHHHHHHCCCC
>Mature Secondary Structure
MEARVIGLEKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTADAYREFLDQSGL
CCCEEECHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
NARIQAELAELNVDDVNALAETGAKIRQWIVETPLTPGLEQEIREAFAALSNGNPDIAVA
CEEEEHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEE
VRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAVKEVFASLFNDRAIAYRVHQGFEHSL
EECCCCHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
VALSAGVQRMVRSETGAAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSK
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCHHHHHHCCCCHHHHHHCC
PLLNAGKHAVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQERQQFALNDHELQELAKQ
HHHHCCHHHHHHHHCCCCHHEEEECCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHH
ALIIEKHYGAPMDIEWAKDGDDGQIYIVQARPETVKSRQNVGTMERYLLKQKGTVLCEGR
HHHHHHCCCCCCEEEECCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHCCCCEEECCC
SIGQRIGSGKVRIVNSIKEMDKVQDGDVLVSDMTDPDWEPVMKRAAAIITNRGGRTCHAA
HHHHHCCCCCEEEHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHH
IIARELGVPAIVGCGNATEVLTDGQEVTVSCAEGDTGFIYEGALDFEVQRNSIESMPKLS
HHHHHCCCCEEEECCCCHHHHCCCCEEEEEEECCCCCEEEECCEEEEECHHHHHCCCCCE
FKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIESLPRETRAAVMT
EEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCHHHEEHHHCCCHHHHHHHH
RTAGYASPVEFYVEKLVEGIATLAAAFADKPVIVRMSDFKSNEYANLIGGKLYEPEEENP
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCC
MLGFRGASRYVSDNFRDCFELECRALKKVRDEMGLTNIQIMIPFVRTVSEAKRVIELLAQ
CEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHH
NGLKRGENGLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLF
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHH
DERDAAVKALLSMAIHACRKAGKYVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDT
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCEECCCCHHHHHH
WFFLAERKNQANLICYKKRPIRGSFFIFRIENFYLCKFTWHVIINKLGLIP
HHHHHHCCCCCCEEEEECCCCCCCEEEEEECCEEEEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]