Click here to switch to the map view.

The map label for this gene is acnB [H]

Identifier: 213158511

GI number: 213158511

Start: 2547080

End: 2549719

Strand: Reverse

Name: acnB [H]

Synonym: AB57_2462

Alternate gene names: 213158511

Gene position: 2549719-2547080 (Counterclockwise)

Preceding gene: 213158512

Following gene: 213158510

Centisome position: 62.95

GC content: 42.65

Gene sequence:

>2640_bases
GTGCTAGAAGCTTACCGCCAACACGTTGCTGAACGTGCCGCACTCGGAGTCCCACCGAAGCCACTTGATGATGCTCAAAC
TGCTCAACTTGTTGAGTTATTAAAAAACCCACCGGCAGGTGAAGAAGCATTCTTGGTTGATTTGCTTGAAAACCGTGTTC
CTGCAGGTGTTGACCAAGCAGCTTACGTAAAAGCAGCTTTCTTGGCAGCGATTGCAAAAGGCGAAGCGACATCTCCGCTA
GTTTCTAAAGAACGTGCAGTTTATTTACTAGGTACGATGCTTGGTGGCTATAACGTAGCGCCTTTAGTTGAGCTTCTGGA
TGATGCTGAATTAGGTAGCTTAGCTGCTGAAGCTTTGAAAAAAACATTACTTGTATTTGATGCGTTCCATGACGTAGCTG
ATAAAGCTAAAGCTGGCAATGCAAATGCAAAAGCTGTTTTACAATCTTGGGCTGATGCTGAATGGTTCACTAGTCGTAAA
GATGTACCAGAAGAAATCAAAATCACTGTGTTCAAAGTAACAGGTGAAACAAACACTGATGACTTGTCTCCTGCTCAAGA
CGCATGGAGCCGTCCAGACATCCCATTGCATGCAAATGCAATGTTGAAAAATGAGCGTGATGGTATCAACCCTGAAAAAC
CAGGTGAAGTTGGTCCATTAAGCCAAATTAAAGAACTCATTGCTAAAGGCAATCAAGTTGCTTATGTTGGTGACGTTGTT
GGTACAGGTTCATCTCGTAAATCTGCAACAAACTCTGTTCTTTGGTTCTTTGGTGATGAAATCGCTCACATTCCAAACAA
AAAAGACGGTGGTGTGTGCTTAGGCGGTAAAATTGCTCCAATCTTCTTTAACACAATGGAAGATGCTGGTGCGTTACCAG
TAGAAATCGATGTTTCTAACATGAACATGGGTGACGAAGTTACTCTTAAAATCGATCATGCTGCTGCAAAAGTTACTGCG
TTCAAAAATGGCGAACAAATCGCTGAGTCTGAACTTAAAACTCCAGTACTTTTAGATGAAGTACGTGCTGGTGGTCGTAT
TAACTTGATTATTGGTCGTGGCTTAACTGCTAAAGCACGTGAAGCTTTAGGTTTAGCTCCATCTACATTATTCCGTACTC
CAGTACAACCAGCTGACACTGGCAAAGGTTTCACTTTAGCTCAGAAGATGGTTGGTCGCGCATGTGGTCTCCCAGAAGGT
CAAGGTATCCGTCCAGGTACTTACTGTGAACCTAAAATGACTACAGTTGGTTCTCAAGATACAACTGGTCCTATGACTCG
TGATGAGTTAAAAGACCTAGCTTGCTTGGGCTTCTCTGCTGACTTAGTAATGCAGTCTTTCTGTCATACTGCTGCTTATC
CAAAGCCAGTTGACGTACAAATGCAACATACACTTCCAGACTTCATCATGAACCGTGGTGGTGTATCTTTACGTCCAGGT
GACGGTATTATCCACTCTTGGTTAAACCGTATGCTTCTTCCAGATACAGTAGGTACTGGTGGTGACTCGCATACTCGTTT
CCCAATTGGTATTTCATTCCCAGCAGGTTCTGGTCTTGTAGCTTTCGCTGCTGCAACTGGTGTAATGCCACTTGATATGC
CTGAATCAGTTCTTGTTAAGTTCAAAGGTAAAATGCAGCCTGGTATCACTTTACGTGACCTTGTACATGCGATTCCTTAC
TATGCAATCAAAGAAGGCGATCTTACTGTTGAGAAAAAAGGTAAGAAAAACATCTTCTCTGGTCGTATCTTAGAAATCGA
CTTAACAGAAATGGAAACTGACTTAACAGTTGAGCAAGCATTCGAACTTTCTGATGCTTCTGCTGAACGTTCAGCTGCTG
GTTGTGCAATCACACTTTCAGAAGAGAAAGTTGCTGAGTACTTACGTTCTAACATCACAATGCTTAAGTGGATGATTTCA
CAAGGCTATGGTGATGCACGTACAATGGCTCGCCGTGTTGAAAACATGGAAAAATGGTTAGCAAACCCAAGCTTACTTAA
AGCTGATGCTGATGCTGAATACACTAAAGTGTATGAAATTGACTTGTCAGAAATCAAAGAACCTATCCTTTGCTGCCCGA
ACGATCCAGATGATGCGAAACTTCTTTCTGACGTTCAAGGCGACAAAATTGATGAAGTATTCATCGGTTCTTGTATGACT
AACATTGGTCACTTCCGTGCTGCTGGTCAGTTACTTGAGAAAGTACCAAGCGGTTCATTAACAACTCGTTTATGGTTGGC
TCCACCAACACGTATGGATGAACATCAGTTAATGGAAGAGGGCTTCTATAACACTTATGGCCGTGCTGGTGCACGTACAG
AAATGCCTGGTTGTTCATTATGTATGGGTAACCAAGCACGTGTTGCGCCGAACACAACTGTTGTTTCGACTTCTACACGT
AACTTCCCTAACCGTTTAGGCCAAGGTTCTAACGTTTACTTAGCGTCTGCTGAGCTTGCATCTGTTGCTGCTGTACTTGG
TAAATTACCAACTCCGGAAGAATACCAACAATATGCAGCTCAAATTGACAGTATGTCTGCTGACATCTACAAATATTTGA
ATTTCGACCAAATGGGCGAATATACAAATGCTGCTGATAAAGTAGATACTAAGAAAATTGCCGCTGCTCAGTTGACTTAA

Upstream 100 bases:

>100_bases
GGCTTGAACTCTAGTAAAATTTTGTAGTCCATATTACTTGTGAAGCTTTGTGAAAGCTGGAATTTACAAGTAATTTTTTA
AAAAAAGAGGAATGAACACC

Downstream 100 bases:

>100_bases
TAGGTCGATTTAGTACAAAAAAAGGAGCTGATTATTCAGCTCCTTTTTATTCTAATGAGAATTGGTACATATTTTAATTG
TTAAAATAATAAATTAATCA

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 879; Mature: 879

Protein sequence:

>879_residues
MLEAYRQHVAERAALGVPPKPLDDAQTAQLVELLKNPPAGEEAFLVDLLENRVPAGVDQAAYVKAAFLAAIAKGEATSPL
VSKERAVYLLGTMLGGYNVAPLVELLDDAELGSLAAEALKKTLLVFDAFHDVADKAKAGNANAKAVLQSWADAEWFTSRK
DVPEEIKITVFKVTGETNTDDLSPAQDAWSRPDIPLHANAMLKNERDGINPEKPGEVGPLSQIKELIAKGNQVAYVGDVV
GTGSSRKSATNSVLWFFGDEIAHIPNKKDGGVCLGGKIAPIFFNTMEDAGALPVEIDVSNMNMGDEVTLKIDHAAAKVTA
FKNGEQIAESELKTPVLLDEVRAGGRINLIIGRGLTAKAREALGLAPSTLFRTPVQPADTGKGFTLAQKMVGRACGLPEG
QGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVQMQHTLPDFIMNRGGVSLRPG
DGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVKFKGKMQPGITLRDLVHAIPY
YAIKEGDLTVEKKGKKNIFSGRILEIDLTEMETDLTVEQAFELSDASAERSAAGCAITLSEEKVAEYLRSNITMLKWMIS
QGYGDARTMARRVENMEKWLANPSLLKADADAEYTKVYEIDLSEIKEPILCCPNDPDDAKLLSDVQGDKIDEVFIGSCMT
NIGHFRAAGQLLEKVPSGSLTTRLWLAPPTRMDEHQLMEEGFYNTYGRAGARTEMPGCSLCMGNQARVAPNTTVVSTSTR
NFPNRLGQGSNVYLASAELASVAAVLGKLPTPEEYQQYAAQIDSMSADIYKYLNFDQMGEYTNAADKVDTKKIAAAQLT

Sequences:

>Translated_879_residues
MLEAYRQHVAERAALGVPPKPLDDAQTAQLVELLKNPPAGEEAFLVDLLENRVPAGVDQAAYVKAAFLAAIAKGEATSPL
VSKERAVYLLGTMLGGYNVAPLVELLDDAELGSLAAEALKKTLLVFDAFHDVADKAKAGNANAKAVLQSWADAEWFTSRK
DVPEEIKITVFKVTGETNTDDLSPAQDAWSRPDIPLHANAMLKNERDGINPEKPGEVGPLSQIKELIAKGNQVAYVGDVV
GTGSSRKSATNSVLWFFGDEIAHIPNKKDGGVCLGGKIAPIFFNTMEDAGALPVEIDVSNMNMGDEVTLKIDHAAAKVTA
FKNGEQIAESELKTPVLLDEVRAGGRINLIIGRGLTAKAREALGLAPSTLFRTPVQPADTGKGFTLAQKMVGRACGLPEG
QGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVQMQHTLPDFIMNRGGVSLRPG
DGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVKFKGKMQPGITLRDLVHAIPY
YAIKEGDLTVEKKGKKNIFSGRILEIDLTEMETDLTVEQAFELSDASAERSAAGCAITLSEEKVAEYLRSNITMLKWMIS
QGYGDARTMARRVENMEKWLANPSLLKADADAEYTKVYEIDLSEIKEPILCCPNDPDDAKLLSDVQGDKIDEVFIGSCMT
NIGHFRAAGQLLEKVPSGSLTTRLWLAPPTRMDEHQLMEEGFYNTYGRAGARTEMPGCSLCMGNQARVAPNTTVVSTSTR
NFPNRLGQGSNVYLASAELASVAAVLGKLPTPEEYQQYAAQIDSMSADIYKYLNFDQMGEYTNAADKVDTKKIAAAQLT
>Mature_879_residues
MLEAYRQHVAERAALGVPPKPLDDAQTAQLVELLKNPPAGEEAFLVDLLENRVPAGVDQAAYVKAAFLAAIAKGEATSPL
VSKERAVYLLGTMLGGYNVAPLVELLDDAELGSLAAEALKKTLLVFDAFHDVADKAKAGNANAKAVLQSWADAEWFTSRK
DVPEEIKITVFKVTGETNTDDLSPAQDAWSRPDIPLHANAMLKNERDGINPEKPGEVGPLSQIKELIAKGNQVAYVGDVV
GTGSSRKSATNSVLWFFGDEIAHIPNKKDGGVCLGGKIAPIFFNTMEDAGALPVEIDVSNMNMGDEVTLKIDHAAAKVTA
FKNGEQIAESELKTPVLLDEVRAGGRINLIIGRGLTAKAREALGLAPSTLFRTPVQPADTGKGFTLAQKMVGRACGLPEG
QGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVQMQHTLPDFIMNRGGVSLRPG
DGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVKFKGKMQPGITLRDLVHAIPY
YAIKEGDLTVEKKGKKNIFSGRILEIDLTEMETDLTVEQAFELSDASAERSAAGCAITLSEEKVAEYLRSNITMLKWMIS
QGYGDARTMARRVENMEKWLANPSLLKADADAEYTKVYEIDLSEIKEPILCCPNDPDDAKLLSDVQGDKIDEVFIGSCMT
NIGHFRAAGQLLEKVPSGSLTTRLWLAPPTRMDEHQLMEEGFYNTYGRAGARTEMPGCSLCMGNQARVAPNTTVVSTSTR
NFPNRLGQGSNVYLASAELASVAAVLGKLPTPEEYQQYAAQIDSMSADIYKYLNFDQMGEYTNAADKVDTKKIAAAQLT

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=868, Percent_Identity=74.7695852534562, Blast_Score=1301, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=414, Percent_Identity=26.0869565217391, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 94756; Mature: 94756

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRQHVAERAALGVPPKPLDDAQTAQLVELLKNPPAGEEAFLVDLLENRVPAGVDQA
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHH
AYVKAAFLAAIAKGEATSPLVSKERAVYLLGTMLGGYNVAPLVELLDDAELGSLAAEALK
HHHHHHHHHHHHCCCCCCCHHHHCCEEEEEEHHHCCCCHHHHHHHHCCCHHHHHHHHHHH
KTLLVFDAFHDVADKAKAGNANAKAVLQSWADAEWFTSRKDVPEEIKITVFKVTGETNTD
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEEEECCCCCC
DLSPAQDAWSRPDIPLHANAMLKNERDGINPEKPGEVGPLSQIKELIAKGNQVAYVGDVV
CCCCHHHHCCCCCCCEECHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECEE
GTGSSRKSATNSVLWFFGDEIAHIPNKKDGGVCLGGKIAPIFFNTMEDAGALPVEIDVSN
CCCCCCCCCCCCEEEEECCHHHHCCCCCCCCEEECCEEEEEEEECHHCCCCCEEEEEECC
MNMGDEVTLKIDHAAAKVTAFKNGEQIAESELKTPVLLDEVRAGGRINLIIGRGLTAKAR
CCCCCEEEEEEECCCEEEEEECCCHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCHHHH
EALGLAPSTLFRTPVQPADTGKGFTLAQKMVGRACGLPEGQGIRPGTYCEPKMTTVGSQD
HHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCC
TTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVQMQHTLPDFIMNRGGVSLRPG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEHHHCCHHHHHCCCCCEECCC
DGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVK
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHE
FKGKMQPGITLRDLVHAIPYYAIKEGDLTVEKKGKKNIFSGRILEIDLTEMETDLTVEQA
ECCCCCCCCCHHHHHHHCCCEEEECCCEEEECCCCCCCCCCEEEEEEHHHCCCCCHHHHH
FELSDASAERSAAGCAITLSEEKVAEYLRSNITMLKWMISQGYGDARTMARRVENMEKWL
HHCCCCCHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ANPSLLKADADAEYTKVYEIDLSEIKEPILCCPNDPDDAKLLSDVQGDKIDEVFIGSCMT
CCCCEEECCCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHH
NIGHFRAAGQLLEKVPSGSLTTRLWLAPPTRMDEHQLMEEGFYNTYGRAGARTEMPGCSL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCE
CMGNQARVAPNTTVVSTSTRNFPNRLGQGSNVYLASAELASVAAVLGKLPTPEEYQQYAA
EECCCCEECCCCEEEECCCCCCHHHHCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHH
QIDSMSADIYKYLNFDQMGEYTNAADKVDTKKIAAAQLT
HHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEAYRQHVAERAALGVPPKPLDDAQTAQLVELLKNPPAGEEAFLVDLLENRVPAGVDQA
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHH
AYVKAAFLAAIAKGEATSPLVSKERAVYLLGTMLGGYNVAPLVELLDDAELGSLAAEALK
HHHHHHHHHHHHCCCCCCCHHHHCCEEEEEEHHHCCCCHHHHHHHHCCCHHHHHHHHHHH
KTLLVFDAFHDVADKAKAGNANAKAVLQSWADAEWFTSRKDVPEEIKITVFKVTGETNTD
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEEEECCCCCC
DLSPAQDAWSRPDIPLHANAMLKNERDGINPEKPGEVGPLSQIKELIAKGNQVAYVGDVV
CCCCHHHHCCCCCCCEECHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECEE
GTGSSRKSATNSVLWFFGDEIAHIPNKKDGGVCLGGKIAPIFFNTMEDAGALPVEIDVSN
CCCCCCCCCCCCEEEEECCHHHHCCCCCCCCEEECCEEEEEEEECHHCCCCCEEEEEECC
MNMGDEVTLKIDHAAAKVTAFKNGEQIAESELKTPVLLDEVRAGGRINLIIGRGLTAKAR
CCCCCEEEEEEECCCEEEEEECCCHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCHHHH
EALGLAPSTLFRTPVQPADTGKGFTLAQKMVGRACGLPEGQGIRPGTYCEPKMTTVGSQD
HHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCC
TTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVQMQHTLPDFIMNRGGVSLRPG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEHHHCCHHHHHCCCCCEECCC
DGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVK
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHE
FKGKMQPGITLRDLVHAIPYYAIKEGDLTVEKKGKKNIFSGRILEIDLTEMETDLTVEQA
ECCCCCCCCCHHHHHHHCCCEEEECCCEEEECCCCCCCCCCEEEEEEHHHCCCCCHHHHH
FELSDASAERSAAGCAITLSEEKVAEYLRSNITMLKWMISQGYGDARTMARRVENMEKWL
HHCCCCCHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ANPSLLKADADAEYTKVYEIDLSEIKEPILCCPNDPDDAKLLSDVQGDKIDEVFIGSCMT
CCCCEEECCCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHH
NIGHFRAAGQLLEKVPSGSLTTRLWLAPPTRMDEHQLMEEGFYNTYGRAGARTEMPGCSL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCE
CMGNQARVAPNTTVVSTSTRNFPNRLGQGSNVYLASAELASVAAVLGKLPTPEEYQQYAA
EECCCCEECCCCEEEECCCCCCHHHHCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHH
QIDSMSADIYKYLNFDQMGEYTNAADKVDTKKIAAAQLT
HHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]