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The map label for this gene is metR [H]

Identifier: 213158505

GI number: 213158505

Start: 2541312

End: 2542232

Strand: Reverse

Name: metR [H]

Synonym: AB57_2456

Alternate gene names: 213158505

Gene position: 2542232-2541312 (Counterclockwise)

Preceding gene: 213158506

Following gene: 213158503

Centisome position: 62.76

GC content: 40.07

Gene sequence:

>921_bases
ATGTTAGAAATTCGACACCTTAAAACTTTAGTTGCTTTACGTGAACATGGTTCACTGGTCGCTGCAGCTAATGACCTATG
CTTAACGCCCTCGGCTATTTCTCACCAATTAAAAGAATTAGATCATTGGTATGGGGTAGAGGTGGTTAATCGCCGCAGTC
GACCTGTTAGTTTTTCTAATGTAGGACAACGCCTACTTAAGTTGGCTGATGATGTATTGCCGCAAATTCAGATTACTCAG
AGTGATATTACACGTATTGTGCACGGACAAACGGGCAGAATTATTTTTTCATCTGAATGTCATAGCTGTTTTGACTGGCT
AATGCCTTTACTCAATCAGTACCGTCAGCAATATCCAGACGTCGATTTAGACTTTGCTTCGGGGTTTGAAGCGAACCCAC
ATGAACTTTTACAAAATGCTGAGTTTGATTTACTTATTACAGCTGATCCGATCGCACTAAAAGGAATCGAATACTTCCCG
ATCTTTGAATATGAGTCGCGTTTGGTTTTATCAAATACTCATCGATTGGTTCGTGCTGAAAATATTACTGTTCAGGATCT
CGCAGAAGAGGTTCTTATTACTTACCCGGTTGATAAGCACCGTTTAGATATCATGTCGAAACTGTTTATACCTGCAAATA
TTCAACCAAAACAAATCCGAACAACAGACTTAACGCAAATGCTTATTCAACTCGTTGCGAGTGGCCGAGGTATTGCTGCT
TTACCAGATTGGGTAGTCAATGAATACGAACAAAAGGGTTGGGTGACTAGCCGCCGTCTAGATTGTGTTTCTCCAACAGG
TTTAAGACGTACATTGTATGCAGGTTATCGAACTGAAGAAAAAGAGAAGAGCTACTTTGAAGGTTTCTTAAAGCAGCTAG
AAAAGTTTTCTTTAAAACGCACTTCTTACTATTCAGGCTAA

Upstream 100 bases:

>100_bases
TATTCATTAGTGATGATTTATCTCCGTTAACTCTGTTTAGTAATAAACAAGAGATAACTTAAAAATTTATAGATAAATTT
ATGTATTCAGGCTTGTGCCC

Downstream 100 bases:

>100_bases
AAGTACTTATTTAGCCTAAGCATTTATTAAAAGGTGGCTTCGGGTGCCTTTTATTTACCTATAAATAAAGATAATAGACC
TGCTGCAATCAGTGGGCCAA

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MLEIRHLKTLVALREHGSLVAAANDLCLTPSAISHQLKELDHWYGVEVVNRRSRPVSFSNVGQRLLKLADDVLPQIQITQ
SDITRIVHGQTGRIIFSSECHSCFDWLMPLLNQYRQQYPDVDLDFASGFEANPHELLQNAEFDLLITADPIALKGIEYFP
IFEYESRLVLSNTHRLVRAENITVQDLAEEVLITYPVDKHRLDIMSKLFIPANIQPKQIRTTDLTQMLIQLVASGRGIAA
LPDWVVNEYEQKGWVTSRRLDCVSPTGLRRTLYAGYRTEEKEKSYFEGFLKQLEKFSLKRTSYYSG

Sequences:

>Translated_306_residues
MLEIRHLKTLVALREHGSLVAAANDLCLTPSAISHQLKELDHWYGVEVVNRRSRPVSFSNVGQRLLKLADDVLPQIQITQ
SDITRIVHGQTGRIIFSSECHSCFDWLMPLLNQYRQQYPDVDLDFASGFEANPHELLQNAEFDLLITADPIALKGIEYFP
IFEYESRLVLSNTHRLVRAENITVQDLAEEVLITYPVDKHRLDIMSKLFIPANIQPKQIRTTDLTQMLIQLVASGRGIAA
LPDWVVNEYEQKGWVTSRRLDCVSPTGLRRTLYAGYRTEEKEKSYFEGFLKQLEKFSLKRTSYYSG
>Mature_306_residues
MLEIRHLKTLVALREHGSLVAAANDLCLTPSAISHQLKELDHWYGVEVVNRRSRPVSFSNVGQRLLKLADDVLPQIQITQ
SDITRIVHGQTGRIIFSSECHSCFDWLMPLLNQYRQQYPDVDLDFASGFEANPHELLQNAEFDLLITADPIALKGIEYFP
IFEYESRLVLSNTHRLVRAENITVQDLAEEVLITYPVDKHRLDIMSKLFIPANIQPKQIRTTDLTQMLIQLVASGRGIAA
LPDWVVNEYEQKGWVTSRRLDCVSPTGLRRTLYAGYRTEEKEKSYFEGFLKQLEKFSLKRTSYYSG

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=291, Percent_Identity=41.5807560137457, Blast_Score=227, Evalue=7e-61,
Organism=Escherichia coli, GI1786401, Length=268, Percent_Identity=23.8805970149254, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790399, Length=300, Percent_Identity=27.6666666666667, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788887, Length=249, Percent_Identity=25.3012048192771, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367136, Length=256, Percent_Identity=22.265625, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI157672245, Length=288, Percent_Identity=20.4861111111111, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788296, Length=190, Percent_Identity=25.7894736842105, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790208, Length=247, Percent_Identity=22.6720647773279, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35140; Mature: 35140

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIRHLKTLVALREHGSLVAAANDLCLTPSAISHQLKELDHWYGVEVVNRRSRPVSFSN
CCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHCCCCCCCCHHH
VGQRLLKLADDVLPQIQITQSDITRIVHGQTGRIIFSSECHSCFDWLMPLLNQYRQQYPD
HHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCC
VDLDFASGFEANPHELLQNAEFDLLITADPIALKGIEYFPIFEYESRLVLSNTHRLVRAE
CCCCCCCCCCCCHHHHHHCCCCEEEEECCCCEECCCEEEEEECCCCEEEEECCHHEEEEC
NITVQDLAEEVLITYPVDKHRLDIMSKLFIPANIQPKQIRTTDLTQMLIQLVASGRGIAA
CCCHHHHHHHHEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
LPDWVVNEYEQKGWVTSRRLDCVSPTGLRRTLYAGYRTEEKEKSYFEGFLKQLEKFSLKR
CCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TSYYSG
HCCCCC
>Mature Secondary Structure
MLEIRHLKTLVALREHGSLVAAANDLCLTPSAISHQLKELDHWYGVEVVNRRSRPVSFSN
CCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHCCCCCCCCHHH
VGQRLLKLADDVLPQIQITQSDITRIVHGQTGRIIFSSECHSCFDWLMPLLNQYRQQYPD
HHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCC
VDLDFASGFEANPHELLQNAEFDLLITADPIALKGIEYFPIFEYESRLVLSNTHRLVRAE
CCCCCCCCCCCCHHHHHHCCCCEEEEECCCCEECCCEEEEEECCCCEEEEECCHHEEEEC
NITVQDLAEEVLITYPVDKHRLDIMSKLFIPANIQPKQIRTTDLTQMLIQLVASGRGIAA
CCCHHHHHHHHEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
LPDWVVNEYEQKGWVTSRRLDCVSPTGLRRTLYAGYRTEEKEKSYFEGFLKQLEKFSLKR
CCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TSYYSG
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]