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The map label for this gene is ytzG [H]

Identifier: 213158501

GI number: 213158501

Start: 2539532

End: 2540236

Strand: Reverse

Name: ytzG [H]

Synonym: AB57_2452

Alternate gene names: 213158501

Gene position: 2540236-2539532 (Counterclockwise)

Preceding gene: 213158503

Following gene: 213158498

Centisome position: 62.71

GC content: 38.16

Gene sequence:

>705_bases
ATGCTGCTGGAAAAAATTTTACAATCACAAGGGTTTGGTTCACGTAAATATTGTCAGCAGTTAATAAAAAATGGTTCAGT
AATTATTGATGGGGAGGTTGTGAGTGATCTCAAAAAGCAGTTCTCCCCAGAAAATTTTGAGTTTTCCGTTTTTGGTCAAA
ATTATCAATATAGAGAAAAAATTTATATTGCTCTAAGAAAACCTCAAGGTTTTGAGTGTTCTCATCAGCCTCAGCATCAT
CAAAGTGTTTTTAGCCTACTGCCTGAGACCATGATTCATCGTGGTGTGCAGGCGGTTGGACGCTTGGATCAAGACACAAC
GGGTCTGCTTTTACTTACTGATGATGGTAAATATCTACAAGCTTTAACTCATCCTCGTAAACATGTACCAAAGGTTTATC
ACGTTACAACTTACGACCCCGTAACCCCTGAGCAAATTGAAATGCTTACTCAGGGTGTTAATTTACACCAAGAAAAGGGG
GTATTTGCTGCCACAGATGTAGCACTACTAGGAACTCATCAATTAACGATGACTATTCATCAAGGTGTTTATCATCAAGT
TAAAAGAATGATTGCAGCCGTAGGCAATAAAGTCGAGAAGTTACATCGACATCAAATTGGTCAATTGGTTTTACCTGAAA
TTGAGGAAGGGGGCTGGGTATATTTGTCGGAGCAGGAAAAACAACTTGCTCAAAATATTATTTAA

Upstream 100 bases:

>100_bases
TTCAAAGTTAATGAAGTTAAATGCGAAGATGATAAAGCAGAAGGTAATAAAACACTAAAATTACGTTAAAATAATGGTTT
CGTTTTAAAGAGAAAGCTTT

Downstream 100 bases:

>100_bases
GAAAACAATGAAATGTCTGAAACTCACTGTCTCGGATGAACCCCATTTTTTCATAGAGGCGGTGAGATTCATGATTATTC
GATTGGGTTTCTAGGCTAAT

Product: 16S rRNA pseudouridylate 516 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MLLEKILQSQGFGSRKYCQQLIKNGSVIIDGEVVSDLKKQFSPENFEFSVFGQNYQYREKIYIALRKPQGFECSHQPQHH
QSVFSLLPETMIHRGVQAVGRLDQDTTGLLLLTDDGKYLQALTHPRKHVPKVYHVTTYDPVTPEQIEMLTQGVNLHQEKG
VFAATDVALLGTHQLTMTIHQGVYHQVKRMIAAVGNKVEKLHRHQIGQLVLPEIEEGGWVYLSEQEKQLAQNII

Sequences:

>Translated_234_residues
MLLEKILQSQGFGSRKYCQQLIKNGSVIIDGEVVSDLKKQFSPENFEFSVFGQNYQYREKIYIALRKPQGFECSHQPQHH
QSVFSLLPETMIHRGVQAVGRLDQDTTGLLLLTDDGKYLQALTHPRKHVPKVYHVTTYDPVTPEQIEMLTQGVNLHQEKG
VFAATDVALLGTHQLTMTIHQGVYHQVKRMIAAVGNKVEKLHRHQIGQLVLPEIEEGGWVYLSEQEKQLAQNII
>Mature_234_residues
MLLEKILQSQGFGSRKYCQQLIKNGSVIIDGEVVSDLKKQFSPENFEFSVFGQNYQYREKIYIALRKPQGFECSHQPQHH
QSVFSLLPETMIHRGVQAVGRLDQDTTGLLLLTDDGKYLQALTHPRKHVPKVYHVTTYDPVTPEQIEMLTQGVNLHQEKG
VFAATDVALLGTHQLTMTIHQGVYHQVKRMIAAVGNKVEKLHRHQIGQLVLPEIEEGGWVYLSEQEKQLAQNII

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=37.8260869565217, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1787524, Length=232, Percent_Identity=31.0344827586207, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1790453, Length=227, Percent_Identity=26.8722466960352, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081838, Length=142, Percent_Identity=31.6901408450704, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 26664; Mature: 26664

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEKILQSQGFGSRKYCQQLIKNGSVIIDGEVVSDLKKQFSPENFEFSVFGQNYQYREK
CHHHHHHHHCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCEEEEEECCCCEEEEE
IYIALRKPQGFECSHQPQHHQSVFSLLPETMIHRGVQAVGRLDQDTTGLLLLTDDGKYLQ
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHH
ALTHPRKHVPKVYHVTTYDPVTPEQIEMLTQGVNLHQEKGVFAATDVALLGTHQLTMTIH
HHHCCHHHCCEEEEEECCCCCCHHHHHHHHHCCCCHHHCCCEEEHHHHHHCCHHHHHHHH
QGVYHQVKRMIAAVGNKVEKLHRHQIGQLVLPEIEEGGWVYLSEQEKQLAQNII
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEECHHHHHHHHHCC
>Mature Secondary Structure
MLLEKILQSQGFGSRKYCQQLIKNGSVIIDGEVVSDLKKQFSPENFEFSVFGQNYQYREK
CHHHHHHHHCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCEEEEEECCCCEEEEE
IYIALRKPQGFECSHQPQHHQSVFSLLPETMIHRGVQAVGRLDQDTTGLLLLTDDGKYLQ
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHH
ALTHPRKHVPKVYHVTTYDPVTPEQIEMLTQGVNLHQEKGVFAATDVALLGTHQLTMTIH
HHHCCHHHCCEEEEEECCCCCCHHHHHHHHHCCCCHHHCCCEEEHHHHHHCCHHHHHHHH
QGVYHQVKRMIAAVGNKVEKLHRHQIGQLVLPEIEEGGWVYLSEQEKQLAQNII
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]