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The map label for this gene is miaA

Identifier: 213158496

GI number: 213158496

Start: 2534180

End: 2535124

Strand: Reverse

Name: miaA

Synonym: AB57_2447

Alternate gene names: 213158496

Gene position: 2535124-2534180 (Counterclockwise)

Preceding gene: 213158497

Following gene: 213158495

Centisome position: 62.59

GC content: 38.62

Gene sequence:

>945_bases
ATGTCAAACCAATTGCCCGTCATCAATTTAATGGGACCGACTGCAAGCGGAAAAACCGCTTTGGCATGTGAACTCTATGA
ACGTGGAAATTTTGAATTAATTTCAGTTGATTCTGCACTTGTATATAAAGATATGGATATTGGTACGGCTAAGCCAACAC
GTGAAGAGCAGGAGCTATACCCACATCATTTAATTGATATTATTACTCCACTTGAGGTCTATTCGGCTGCTCAGTTTGTT
GAAGATGCTTGTGCTTTAATTGATGAGATGCATTCACGTGGCAAAACTCCTATTTTGGTAGGAGGAACCATGCTGTATTT
CAAAGCTCTCTTAGAAGGATTATCAAGTAACTTGCCAAGTGCAGATGCAAATGTTCGCGCAGCAATTGAAGAAAAAGCAG
CAAATGAAGGGTGGCAAGCTGTTTATGATGAGCTGGTGGCAGTAGATCCGGCGGCTGGAGTAAAGTTTAAGGTTTCTGAT
AAGCAAAGAATTATTCGGGCGTTGGAAGTTTACCGTATTACAGGTCAACCAATTACCAAATTGCAGGCAGAACAACCAAA
AAATGTACCATATCGATACACATTTCATAACTACGCTTTACTTCCAGATCGGTTAGAATTACATCAACGCATTGAGCAAA
GATTAAGCAAAATGTGGGATATCGGTTTTTTGAGTGAAGTCGAGTCTCTAATTGAAAAATACGATTTAGATGAAAATTTA
CCCTCTATGCGTTCTGTTGGTTATCGACAAGCTCTAGAATTTCTATTAAAAAGTGATATGAGTCTCAAAAAAAAGCAGGA
AATGGAGGATAAAGCCTTATTTGCAACACGACAACTTGCCAAACGTCAATATACGTGGTTACGTTCTTTGCAAGAAATAC
ACGATTTTAAAACTTACTTGACGATAAAGCAGGCGAAAGAAGACTTGCGAAACTCTTATGGATAA

Upstream 100 bases:

>100_bases
AACCGAATTTGCAAGCCAATGTAACCATGGTCGCCCAACTTGGCGTGCATTTCCATTATCTCAACTAGATAAATTATTTG
CTCGAGGAGAGTAGTTTTAC

Downstream 100 bases:

>100_bases
AGCAAAATTTGCACACTGTCTTTTTATAATTTTTAGTTGAAAAATAAAGATAGTTTTTTTGCGCTGATTTAAAAATTTTT
TTTTGGAGTTAAAAATGTCT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSNQLPVINLMGPTASGKTALACELYERGNFELISVDSALVYKDMDIGTAKPTREEQELYPHHLIDIITPLEVYSAAQFV
EDACALIDEMHSRGKTPILVGGTMLYFKALLEGLSSNLPSADANVRAAIEEKAANEGWQAVYDELVAVDPAAGVKFKVSD
KQRIIRALEVYRITGQPITKLQAEQPKNVPYRYTFHNYALLPDRLELHQRIEQRLSKMWDIGFLSEVESLIEKYDLDENL
PSMRSVGYRQALEFLLKSDMSLKKKQEMEDKALFATRQLAKRQYTWLRSLQEIHDFKTYLTIKQAKEDLRNSYG

Sequences:

>Translated_314_residues
MSNQLPVINLMGPTASGKTALACELYERGNFELISVDSALVYKDMDIGTAKPTREEQELYPHHLIDIITPLEVYSAAQFV
EDACALIDEMHSRGKTPILVGGTMLYFKALLEGLSSNLPSADANVRAAIEEKAANEGWQAVYDELVAVDPAAGVKFKVSD
KQRIIRALEVYRITGQPITKLQAEQPKNVPYRYTFHNYALLPDRLELHQRIEQRLSKMWDIGFLSEVESLIEKYDLDENL
PSMRSVGYRQALEFLLKSDMSLKKKQEMEDKALFATRQLAKRQYTWLRSLQEIHDFKTYLTIKQAKEDLRNSYG
>Mature_313_residues
SNQLPVINLMGPTASGKTALACELYERGNFELISVDSALVYKDMDIGTAKPTREEQELYPHHLIDIITPLEVYSAAQFVE
DACALIDEMHSRGKTPILVGGTMLYFKALLEGLSSNLPSADANVRAAIEEKAANEGWQAVYDELVAVDPAAGVKFKVSDK
QRIIRALEVYRITGQPITKLQAEQPKNVPYRYTFHNYALLPDRLELHQRIEQRLSKMWDIGFLSEVESLIEKYDLDENLP
SMRSVGYRQALEFLLKSDMSLKKKQEMEDKALFATRQLAKRQYTWLRSLQEIHDFKTYLTIKQAKEDLRNSYG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=27.7955271565495, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1790613, Length=288, Percent_Identity=52.0833333333333, Blast_Score=270, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI25144712, Length=249, Percent_Identity=30.1204819277108, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=291, Percent_Identity=29.8969072164948, Blast_Score=129, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24649830, Length=295, Percent_Identity=29.8305084745763, Blast_Score=128, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_ACIB3 (B7H141)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002325255.1
- ProteinModelPortal:   B7H141
- SMR:   B7H141
- GeneID:   7059778
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_001352
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35827; Mature: 35696

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQLPVINLMGPTASGKTALACELYERGNFELISVDSALVYKDMDIGTAKPTREEQELY
CCCCCCEEEEECCCCCCCCEEEEEHHHCCCEEEEEECCEEEEECCCCCCCCCCCHHHHHH
PHHLIDIITPLEVYSAAQFVEDACALIDEMHSRGKTPILVGGTMLYFKALLEGLSSNLPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCCCC
ADANVRAAIEEKAANEGWQAVYDELVAVDPAAGVKFKVSDKQRIIRALEVYRITGQPITK
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCCCHHH
LQAEQPKNVPYRYTFHNYALLPDRLELHQRIEQRLSKMWDIGFLSEVESLIEKYDLDENL
HCCCCCCCCCEEEEECCCEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
PSMRSVGYRQALEFLLKSDMSLKKKQEMEDKALFATRQLAKRQYTWLRSLQEIHDFKTYL
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIKQAKEDLRNSYG
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SNQLPVINLMGPTASGKTALACELYERGNFELISVDSALVYKDMDIGTAKPTREEQELY
CCCCCEEEEECCCCCCCCEEEEEHHHCCCEEEEEECCEEEEECCCCCCCCCCCHHHHHH
PHHLIDIITPLEVYSAAQFVEDACALIDEMHSRGKTPILVGGTMLYFKALLEGLSSNLPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCCCC
ADANVRAAIEEKAANEGWQAVYDELVAVDPAAGVKFKVSDKQRIIRALEVYRITGQPITK
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCCCHHH
LQAEQPKNVPYRYTFHNYALLPDRLELHQRIEQRLSKMWDIGFLSEVESLIEKYDLDENL
HCCCCCCCCCEEEEECCCEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
PSMRSVGYRQALEFLLKSDMSLKKKQEMEDKALFATRQLAKRQYTWLRSLQEIHDFKTYL
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIKQAKEDLRNSYG
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA