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The map label for this gene is nasD [H]

Identifier: 213158404

GI number: 213158404

Start: 2434077

End: 2435699

Strand: Reverse

Name: nasD [H]

Synonym: AB57_2352

Alternate gene names: 213158404

Gene position: 2435699-2434077 (Counterclockwise)

Preceding gene: 213158405

Following gene: 213158403

Centisome position: 60.13

GC content: 42.88

Gene sequence:

>1623_bases
ATGAATATTGTACAAGACAAAAATATGCTTCCTGATGATCAATGGATTGACGTTTGTGCCCTCGATGATTTAACCCCAAA
CACGGGTGCAGGAGCGCTAGTGGGCGGTCAAGCGGTTGCAATCTTCCGTGTGGGGCATGAAAAACGTGTTTATGTACTGA
GCAATAAAGATCCGTTTAGCCAAGCGAATGTGATGAGCCGCGGGATTATTGGTGATTTACAAGGCGAACGCGTCGTTGCA
TCACCAATTTATAAACAACATTTCAGTTTAGCTACGGGCCGTTGTTTAGAAGATAAAGACCAAAAATTGGCAGTTTATCC
GAGCAAAATTGTTGATGGCCGTGTTTGGGTCAACGCGGTGCCGCAAAAAACTTATATTACCAATACAGGTGTATCACAAG
ACAAACTTAGACTGGTGCTGATCGGGAATGGTTTGGCAGGGATGCGTTGTCTGGAAGATTTACTCGATATGGCACCAGAC
CGTTATGAAGTTACGGTGATTGGTGAAGAACCATGGGGTAACTATAACCGTATTATGTTGTCTCCGGTTTTATCGGGTGA
GAAAACCATTGAAGATATTATGTTGCATCCACCGAAGTGGTACGACGATAAAGGCATTAAATTTATTGCTGGTGATAAAG
CCGTAAAAATTGATCGTCCGCGTAAAGTGGTTTACACCGAAAAGGGCCAGACAGTTGATTATGACCGTTTGATTCTGGCA
ACAGGCTCGGCACCATTTATTCCACCAGTTCAAGGTGTTGACCTAAAAGGGGTGTTAACTTTCCGCGATATTTATGACGT
TAACACCATGATTGAATACTGTGGTTCAAAAACTAATGCAGTCGTGATTGGTGGCGGTTTGCTGGGTTTAGAAGCTGCCT
ATGGATTAAAGCAACGTGGTATGAATGTCACAGTTCTACATTTGATGGACCGTATTATGGAACGTCAACTTGATGGGCGT
GCGAGTCAATTACTTCGTCATAGCATTGAGCAAAAAGGCATTCAAATCATTACCGAAGCCAATACAGAAGCGTTAATTGG
TGATGAAAACGGTCATGTCAAACAAATCCGTTTGAAAGATGGCACAGTGTTAGATGCGGACCTTGTGGTGTTTGCTGTAG
GTATCCGTCCGAATATTGCTTTGGCGCAAAGTGCAGGATTACGTTGTAACCGTGGTGTATTGGTGAATGACACCATGCAA
ACATTTGACCCAAGTATTTATGCCGTGGGTGAGTGTATCGAACATCGTGGTCAAACTTTCGGTTTGGTTGAACCACTGTG
GGGCCAAGCTTTTATTTGCGCGACCCATTTAGCAGAGCACGGCAGCTTAACTTTTAAAGCACCGACCGTACCGACTCAGT
TAAAAGTCAGTGGTGTCGATGTATTTTCGGCAGGCAATTTTGAACCGAAAGATGATTATGAAGACATTATCCTGAACGAT
GAAAAACGCCAGATTTATAAGCGTATTATCATTCAAAACGACCGGGTAATCGGTGCAGTATTGTTTGGAGATACTGAAGA
TGGCATGTGGTATGCAGAGTTAATTGCAGATCAAACTCCTGTTTCTTCATTTAGAAATAAGCTGCTATTTGGTCGAGATT
TTGCATTAAAAAATGCAGGCTAA

Upstream 100 bases:

>100_bases
CATTCAATTTACAACTGAACGTGGTCAAATCCGCCCATTAACTGAAGCTGAACGTTCAGAAGATCGCATTCCAATGGTTG
AAGCATAAAGGAGAACTTAT

Downstream 100 bases:

>100_bases
ATAGGGAAAGGAGCAGTTATGAATAGTATTCCAAGCGTTGAAATCGATAGCTCCGACCATGTTGCAAAAACAACTAAAAC
AACATGTCCATATTGCGGTG

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MNIVQDKNMLPDDQWIDVCALDDLTPNTGAGALVGGQAVAIFRVGHEKRVYVLSNKDPFSQANVMSRGIIGDLQGERVVA
SPIYKQHFSLATGRCLEDKDQKLAVYPSKIVDGRVWVNAVPQKTYITNTGVSQDKLRLVLIGNGLAGMRCLEDLLDMAPD
RYEVTVIGEEPWGNYNRIMLSPVLSGEKTIEDIMLHPPKWYDDKGIKFIAGDKAVKIDRPRKVVYTEKGQTVDYDRLILA
TGSAPFIPPVQGVDLKGVLTFRDIYDVNTMIEYCGSKTNAVVIGGGLLGLEAAYGLKQRGMNVTVLHLMDRIMERQLDGR
ASQLLRHSIEQKGIQIITEANTEALIGDENGHVKQIRLKDGTVLDADLVVFAVGIRPNIALAQSAGLRCNRGVLVNDTMQ
TFDPSIYAVGECIEHRGQTFGLVEPLWGQAFICATHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGNFEPKDDYEDIILND
EKRQIYKRIIIQNDRVIGAVLFGDTEDGMWYAELIADQTPVSSFRNKLLFGRDFALKNAG

Sequences:

>Translated_540_residues
MNIVQDKNMLPDDQWIDVCALDDLTPNTGAGALVGGQAVAIFRVGHEKRVYVLSNKDPFSQANVMSRGIIGDLQGERVVA
SPIYKQHFSLATGRCLEDKDQKLAVYPSKIVDGRVWVNAVPQKTYITNTGVSQDKLRLVLIGNGLAGMRCLEDLLDMAPD
RYEVTVIGEEPWGNYNRIMLSPVLSGEKTIEDIMLHPPKWYDDKGIKFIAGDKAVKIDRPRKVVYTEKGQTVDYDRLILA
TGSAPFIPPVQGVDLKGVLTFRDIYDVNTMIEYCGSKTNAVVIGGGLLGLEAAYGLKQRGMNVTVLHLMDRIMERQLDGR
ASQLLRHSIEQKGIQIITEANTEALIGDENGHVKQIRLKDGTVLDADLVVFAVGIRPNIALAQSAGLRCNRGVLVNDTMQ
TFDPSIYAVGECIEHRGQTFGLVEPLWGQAFICATHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGNFEPKDDYEDIILND
EKRQIYKRIIIQNDRVIGAVLFGDTEDGMWYAELIADQTPVSSFRNKLLFGRDFALKNAG
>Mature_540_residues
MNIVQDKNMLPDDQWIDVCALDDLTPNTGAGALVGGQAVAIFRVGHEKRVYVLSNKDPFSQANVMSRGIIGDLQGERVVA
SPIYKQHFSLATGRCLEDKDQKLAVYPSKIVDGRVWVNAVPQKTYITNTGVSQDKLRLVLIGNGLAGMRCLEDLLDMAPD
RYEVTVIGEEPWGNYNRIMLSPVLSGEKTIEDIMLHPPKWYDDKGIKFIAGDKAVKIDRPRKVVYTEKGQTVDYDRLILA
TGSAPFIPPVQGVDLKGVLTFRDIYDVNTMIEYCGSKTNAVVIGGGLLGLEAAYGLKQRGMNVTVLHLMDRIMERQLDGR
ASQLLRHSIEQKGIQIITEANTEALIGDENGHVKQIRLKDGTVLDADLVVFAVGIRPNIALAQSAGLRCNRGVLVNDTMQ
TFDPSIYAVGECIEHRGQTFGLVEPLWGQAFICATHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGNFEPKDDYEDIILND
EKRQIYKRIIIQNDRVIGAVLFGDTEDGMWYAELIADQTPVSSFRNKLLFGRDFALKNAG

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=384, Percent_Identity=27.6041666666667, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI226437568, Length=384, Percent_Identity=27.6041666666667, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI65787454, Length=384, Percent_Identity=27.6041666666667, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI4757732, Length=241, Percent_Identity=26.9709543568465, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI22202629, Length=241, Percent_Identity=26.9709543568465, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1789765, Length=380, Percent_Identity=38.9473684210526, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1789065, Length=382, Percent_Identity=26.7015706806283, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789766, Length=102, Percent_Identity=47.0588235294118, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788892, Length=249, Percent_Identity=26.1044176706827, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1787352, Length=274, Percent_Identity=27.7372262773723, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082354, Length=190, Percent_Identity=31.5789473684211, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=400, Percent_Identity=26.5, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24639257, Length=285, Percent_Identity=25.9649122807018, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI281359715, Length=280, Percent_Identity=26.0714285714286, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281359713, Length=280, Percent_Identity=26.0714285714286, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24639250, Length=280, Percent_Identity=26.0714285714286, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI18543267, Length=280, Percent_Identity=26.0714285714286, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24639252, Length=280, Percent_Identity=26.0714285714286, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585130, Length=281, Percent_Identity=24.9110320284698, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24581020, Length=208, Percent_Identity=25.9615384615385, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28573993, Length=208, Percent_Identity=25.9615384615385, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 59636; Mature: 59636

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVQDKNMLPDDQWIDVCALDDLTPNTGAGALVGGQAVAIFRVGHEKRVYVLSNKDPFS
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCEEEEEEECCCEEEEEEECCCCCH
QANVMSRGIIGDLQGERVVASPIYKQHFSLATGRCLEDKDQKLAVYPSKIVDGRVWVNAV
HHHHHHCCCEECCCCCEEEECHHHHHHHHHHCCCCCCCCCCEEEEECHHHCCCEEEEEEC
PQKTYITNTGVSQDKLRLVLIGNGLAGMRCLEDLLDMAPDRYEVTVIGEEPWGNYNRIML
CCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEE
SPVLSGEKTIEDIMLHPPKWYDDKGIKFIAGDKAVKIDRPRKVVYTEKGQTVDYDRLILA
EECCCCCHHHHHHHCCCCCCCCCCCCEEEECCCEEEECCCCEEEEECCCCEECCCEEEEE
TGSAPFIPPVQGVDLKGVLTFRDIYDVNTMIEYCGSKTNAVVIGGGLLGLEAAYGLKQRG
ECCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCC
MNVTVLHLMDRIMERQLDGRASQLLRHSIEQKGIQIITEANTEALIGDENGHVKQIRLKD
CCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCEEEEECCCCCEEEEEECC
GTVLDADLVVFAVGIRPNIALAQSAGLRCNRGVLVNDTMQTFDPSIYAVGECIEHRGQTF
CCEEECCEEEEEECCCCCEEEECCCCCEECCCEEEECCHHHHCCCHHHHHHHHHHCCCEE
GLVEPLWGQAFICATHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGNFEPKDDYEDIILND
ECCCCCCCCEEHHHHHHHHCCCEEEECCCCCCEEEEECEEEEECCCCCCCCCCCCEEECC
EKRQIYKRIIIQNDRVIGAVLFGDTEDGMWYAELIADQTPVSSFRNKLLFGRDFALKNAG
HHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEHHHCCCCCHHHHHHHEEECCCEECCCCC
>Mature Secondary Structure
MNIVQDKNMLPDDQWIDVCALDDLTPNTGAGALVGGQAVAIFRVGHEKRVYVLSNKDPFS
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCEEEEEEECCCEEEEEEECCCCCH
QANVMSRGIIGDLQGERVVASPIYKQHFSLATGRCLEDKDQKLAVYPSKIVDGRVWVNAV
HHHHHHCCCEECCCCCEEEECHHHHHHHHHHCCCCCCCCCCEEEEECHHHCCCEEEEEEC
PQKTYITNTGVSQDKLRLVLIGNGLAGMRCLEDLLDMAPDRYEVTVIGEEPWGNYNRIML
CCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEE
SPVLSGEKTIEDIMLHPPKWYDDKGIKFIAGDKAVKIDRPRKVVYTEKGQTVDYDRLILA
EECCCCCHHHHHHHCCCCCCCCCCCCEEEECCCEEEECCCCEEEEECCCCEECCCEEEEE
TGSAPFIPPVQGVDLKGVLTFRDIYDVNTMIEYCGSKTNAVVIGGGLLGLEAAYGLKQRG
ECCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCC
MNVTVLHLMDRIMERQLDGRASQLLRHSIEQKGIQIITEANTEALIGDENGHVKQIRLKD
CCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCEEEEECCCCCEEEEEECC
GTVLDADLVVFAVGIRPNIALAQSAGLRCNRGVLVNDTMQTFDPSIYAVGECIEHRGQTF
CCEEECCEEEEEECCCCCEEEECCCCCEECCCEEEECCHHHHCCCHHHHHHHHHHCCCEE
GLVEPLWGQAFICATHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGNFEPKDDYEDIILND
ECCCCCCCCEEHHHHHHHHCCCEEEECCCCCCEEEEECEEEEECCCCCCCCCCCCEEECC
EKRQIYKRIIIQNDRVIGAVLFGDTEDGMWYAELIADQTPVSSFRNKLLFGRDFALKNAG
HHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEHHHCCCCCHHHHHHHEEECCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]