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The map label for this gene is argF [H]

Identifier: 213158379

GI number: 213158379

Start: 2410531

End: 2411451

Strand: Direct

Name: argF [H]

Synonym: AB57_2326

Alternate gene names: 213158379

Gene position: 2410531-2411451 (Clockwise)

Preceding gene: 213158378

Following gene: 213158381

Centisome position: 59.51

GC content: 38.55

Gene sequence:

>921_bases
ATGGCTCTAAGACATTTCCTGACTTTACGTGATCTATCCACTCTAGAACTTAATCGCATTTTGGAACGCGCAAGCGAACT
TAAGAAAATGCAGCAAAGCAATAAGGTATATCAACCTTTTGTTGGTAAAGTATTGGGAATGATTTTTGAAAAATCGAGCA
CACGCACTCGTATTTCTTTTGAAGCTGGTATTAATCAATTTGGTGGTAGTGCCATTTTCTTATCACCACGTGATACGCAA
TTAGGCCGTGGTGAACCGATTGAAGATTCAGCACGTGTTATTTCAAGCATGCTTGATATCGTAATGATTCGTACTTTTGG
TCACGATATCGTTGAACGTTTTGCATCTTATTCAAAAGTTCCTGTTATCAATGGCTTAACTGACGATCACCATCCTTGTC
AGTTACTTGCAGATTTACAAACTTATATTGAACATCGCGGCAGTATTGAAGGCAAAACCGTAGCTTGGATTGGTGATGGC
AATAATATGTGTAATTCATACATTGAAGCTGCACACATGATGGGTTTCAAACTCAAGATTGCATCACCAAAAGGTTATGA
ACCTAAACCTGAGTTTTTAGCAGAGTTTGGGCATTGTGTTGAACTTTTTGATAATGCGGAAGATGCGGCTGTAAATGCTG
ATCTTATCGTTACAGATGTATGGGCAAGCATGGGACAAGAAGAAGAACAGAAACTTCGTGAAAAAGCCTTTGCTAATTTT
CAGGTTAACGAGAAATTAATGGGACTTGCACATCCGGACTGTTTATTTATGCATTGCTTACCTGCTCATCGTGGTGAAGA
AATTTCTGAAACCATGCTCGACCATAAAAATGCCGTAGTATGGGACGAAGCAGAAAACCGTCTGCATGCACAAAAAGCTT
TAATGGAATTTTTACTCAACGAAAATTTGAAAAAAGCTTAA

Upstream 100 bases:

>100_bases
TTGAAATGCTTATAGTATAGTAGACTTCTTTTTTCCCACTTCCACCCAGCCAACAACAGATAATTGTTGTAATTCAAAAC
AATTATTATGGAGTAGCTAC

Downstream 100 bases:

>100_bases
TTTCTAATTCAAAAAACAGCATCGTTAAGGTGCTGTTTTTTTATTTTTATAAGATCCCTATTACCCTCATTTATTATTTT
AAAAATTTTTATGATGTTGC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MALRHFLTLRDLSTLELNRILERASELKKMQQSNKVYQPFVGKVLGMIFEKSSTRTRISFEAGINQFGGSAIFLSPRDTQ
LGRGEPIEDSARVISSMLDIVMIRTFGHDIVERFASYSKVPVINGLTDDHHPCQLLADLQTYIEHRGSIEGKTVAWIGDG
NNMCNSYIEAAHMMGFKLKIASPKGYEPKPEFLAEFGHCVELFDNAEDAAVNADLIVTDVWASMGQEEEQKLREKAFANF
QVNEKLMGLAHPDCLFMHCLPAHRGEEISETMLDHKNAVVWDEAENRLHAQKALMEFLLNENLKKA

Sequences:

>Translated_306_residues
MALRHFLTLRDLSTLELNRILERASELKKMQQSNKVYQPFVGKVLGMIFEKSSTRTRISFEAGINQFGGSAIFLSPRDTQ
LGRGEPIEDSARVISSMLDIVMIRTFGHDIVERFASYSKVPVINGLTDDHHPCQLLADLQTYIEHRGSIEGKTVAWIGDG
NNMCNSYIEAAHMMGFKLKIASPKGYEPKPEFLAEFGHCVELFDNAEDAAVNADLIVTDVWASMGQEEEQKLREKAFANF
QVNEKLMGLAHPDCLFMHCLPAHRGEEISETMLDHKNAVVWDEAENRLHAQKALMEFLLNENLKKA
>Mature_305_residues
ALRHFLTLRDLSTLELNRILERASELKKMQQSNKVYQPFVGKVLGMIFEKSSTRTRISFEAGINQFGGSAIFLSPRDTQL
GRGEPIEDSARVISSMLDIVMIRTFGHDIVERFASYSKVPVINGLTDDHHPCQLLADLQTYIEHRGSIEGKTVAWIGDGN
NMCNSYIEAAHMMGFKLKIASPKGYEPKPEFLAEFGHCVELFDNAEDAAVNADLIVTDVWASMGQEEEQKLREKAFANFQ
VNEKLMGLAHPDCLFMHCLPAHRGEEISETMLDHKNAVVWDEAENRLHAQKALMEFLLNENLKKA

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=306, Percent_Identity=40.1960784313725, Blast_Score=226, Evalue=1e-59,
Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=27.2131147540984, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1790703, Length=325, Percent_Identity=38.1538461538462, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI1786469, Length=331, Percent_Identity=37.1601208459215, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI2367364, Length=315, Percent_Identity=27.6190476190476, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI48994908, Length=369, Percent_Identity=23.8482384823848, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=319, Percent_Identity=25.0783699059561, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=321, Percent_Identity=34.8909657320872, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6322331, Length=309, Percent_Identity=26.8608414239482, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24642586, Length=307, Percent_Identity=27.6872964169381, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 34556; Mature: 34425

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRHFLTLRDLSTLELNRILERASELKKMQQSNKVYQPFVGKVLGMIFEKSSTRTRISF
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEH
EAGINQFGGSAIFLSPRDTQLGRGEPIEDSARVISSMLDIVMIRTFGHDIVERFASYSKV
HHCHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVINGLTDDHHPCQLLADLQTYIEHRGSIEGKTVAWIGDGNNMCNSYIEAAHMMGFKLKI
CEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCEEEEE
ASPKGYEPKPEFLAEFGHCVELFDNAEDAAVNADLIVTDVWASMGQEEEQKLREKAFANF
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
QVNEKLMGLAHPDCLFMHCLPAHRGEEISETMLDHKNAVVWDEAENRLHAQKALMEFLLN
CCCHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHH
ENLKKA
CCCCCC
>Mature Secondary Structure 
ALRHFLTLRDLSTLELNRILERASELKKMQQSNKVYQPFVGKVLGMIFEKSSTRTRISF
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEH
EAGINQFGGSAIFLSPRDTQLGRGEPIEDSARVISSMLDIVMIRTFGHDIVERFASYSKV
HHCHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVINGLTDDHHPCQLLADLQTYIEHRGSIEGKTVAWIGDGNNMCNSYIEAAHMMGFKLKI
CEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCEEEEE
ASPKGYEPKPEFLAEFGHCVELFDNAEDAAVNADLIVTDVWASMGQEEEQKLREKAFANF
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
QVNEKLMGLAHPDCLFMHCLPAHRGEEISETMLDHKNAVVWDEAENRLHAQKALMEFLLN
CCCHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHH
ENLKKA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA