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The map label for this gene is tmk

Identifier: 213158250

GI number: 213158250

Start: 3065511

End: 3066110

Strand: Reverse

Name: tmk

Synonym: AB57_2969

Alternate gene names: 213158250

Gene position: 3066110-3065511 (Counterclockwise)

Preceding gene: 213158251

Following gene: 213158249

Centisome position: 75.7

GC content: 40.0

Gene sequence:

>600_bases
ATGTTTATTAGCTTTGAAGGCACGGAAGGGGTAGGAAAAACTACACTCATTCGAAAAATTCATCAGCATTTTGAAGAACA
AGGTAAACAAGTTGTTTTAACTCGAGAGCCGGGTGGAACACCGTTAGCTGAGCAAATCCGTTCGATGCTTTTGGCAGTGA
ATCATAATGAAAATATGAGTCATGATACTGAGTTGCTCTTAATTTATGCGGCACGAGCTCAACACTTACAACAAGTTATT
TTGCCGGCATTAGAGTCTAATAAAATTGTATTAAGTGACCGTTTTACCGATGCGAGTTTTGCTTATCAATGTTCCGGTCG
TGGTTTAAGCCAAGATAAGTTACAGCTTTTAAATCAGAACTTTGTTTCAAGTATGCCAGAGGTGACTTTCTGGTTAGATG
CACCGATTGAATTGGGCATGAACCGAGCACGCGAACGCGGAGCTTTAGATCGTTTTGAACAGGAAAAGTTAAGCTTCTTT
ACTAAGGTTCGTGAAGGATATGAAACTTTATGGAAAGCTGAGCCTGAGCGTATTAAACGTTTAGATGCTACCCAAAGTCC
TGATCAGGTATTCGAGCAAGCTTTGCAATATTTAGCATAG

Upstream 100 bases:

>100_bases
TGGTAATGGTGGACATAAATTTACGGCAGACTTGCAAGCACATAACCAAGCAGTGCAAGAGTATTTATCCGTGTTGAGAT
CGAAGAAATAAGGAAAAGAG

Downstream 100 bases:

>100_bases
GCCATGGGATTAGCCACTCTCATATTTTTTTGGGAGTGGTGAAAAATGACAAAAAATCTGGTTTTAAACGTCATAGAAAA
TATTGGGCAGTATCGTATGC

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MFISFEGTEGVGKTTLIRKIHQHFEEQGKQVVLTREPGGTPLAEQIRSMLLAVNHNENMSHDTELLLIYAARAQHLQQVI
LPALESNKIVLSDRFTDASFAYQCSGRGLSQDKLQLLNQNFVSSMPEVTFWLDAPIELGMNRARERGALDRFEQEKLSFF
TKVREGYETLWKAEPERIKRLDATQSPDQVFEQALQYLA

Sequences:

>Translated_199_residues
MFISFEGTEGVGKTTLIRKIHQHFEEQGKQVVLTREPGGTPLAEQIRSMLLAVNHNENMSHDTELLLIYAARAQHLQQVI
LPALESNKIVLSDRFTDASFAYQCSGRGLSQDKLQLLNQNFVSSMPEVTFWLDAPIELGMNRARERGALDRFEQEKLSFF
TKVREGYETLWKAEPERIKRLDATQSPDQVFEQALQYLA
>Mature_199_residues
MFISFEGTEGVGKTTLIRKIHQHFEEQGKQVVLTREPGGTPLAEQIRSMLLAVNHNENMSHDTELLLIYAARAQHLQQVI
LPALESNKIVLSDRFTDASFAYQCSGRGLSQDKLQLLNQNFVSSMPEVTFWLDAPIELGMNRARERGALDRFEQEKLSFF
TKVREGYETLWKAEPERIKRLDATQSPDQVFEQALQYLA

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=195, Percent_Identity=40.5128205128205, Blast_Score=137, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_ACIB3 (B7GYE0)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002324830.1
- ProteinModelPortal:   B7GYE0
- SMR:   B7GYE0
- GeneID:   7059776
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_000917
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- ProtClustDB:   PRK00698
- HAMAP:   MF_00165
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 22770; Mature: 22770

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFISFEGTEGVGKTTLIRKIHQHFEEQGKQVVLTREPGGTPLAEQIRSMLLAVNHNENMS
CEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCC
HDTELLLIYAARAQHLQQVILPALESNKIVLSDRFTDASFAYQCSGRGLSQDKLQLLNQN
CCHHEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHH
FVSSMPEVTFWLDAPIELGMNRARERGALDRFEQEKLSFFTKVREGYETLWKAEPERIKR
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LDATQSPDQVFEQALQYLA
HCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MFISFEGTEGVGKTTLIRKIHQHFEEQGKQVVLTREPGGTPLAEQIRSMLLAVNHNENMS
CEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCC
HDTELLLIYAARAQHLQQVILPALESNKIVLSDRFTDASFAYQCSGRGLSQDKLQLLNQN
CCHHEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHH
FVSSMPEVTFWLDAPIELGMNRARERGALDRFEQEKLSFFTKVREGYETLWKAEPERIKR
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LDATQSPDQVFEQALQYLA
HCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA