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The map label for this gene is nadB [H]

Identifier: 213158248

GI number: 213158248

Start: 3063253

End: 3064902

Strand: Direct

Name: nadB [H]

Synonym: AB57_2967

Alternate gene names: 213158248

Gene position: 3063253-3064902 (Clockwise)

Preceding gene: 213158234

Following gene: 213158253

Centisome position: 75.63

GC content: 42.24

Gene sequence:

>1650_bases
ATGCGAATGGACATGCCTAATCAACAGACAATTCACCACTTTGACGTGATTATTGTGGGCAGTGGCGGTGCTGGTTTAAG
TCTGGCTTTATCGTTACCAAGTCATTTTAATATTGCAGTTTTAGCTAAGGCTGCTTTAACTGAAGCAAGTACCTTCTATG
CACAAGGTGGTGTTGCTGCAGTGCTTGATGAAACTGACTCTATTCAACAACACATTAATGACACTATGATTGCAGGTGCT
CATTTATGTGAAATGGATGCAGTCCAGCATACTGTAGAAGGCGGTCGCCCTTCTGTTGATTTCCTTTTAAAGCAAGGTGT
GCAGTTTACCTTAGATGAAGATGAACAGCTTCATTTGACTCGTGAGGGTGGCCATTCGCAGCGCCGTATTATTCATTCTG
CTGATGCAACTGGCAAAGCTATTTCAACCACTTTGGTTGAACGCGCCAAAGAACGTAAAAATATCACTATTTTTGAAAAC
TATATTGCGATTGATTTAATCACGTCGCATAAATTGGGTCATACAGATCAAGCGAATCGCGCTATTGGTTTGTATGCACT
AGATGAAAGTACTGAAAAAGTTCATACCTTCCTTGCCCCATTTACCGCACTTGCTTGCGGTGGCGCAATGAAAGCTTACC
TTTATACGTCTAATCCGGATATTGCTACTGGTGACGGTATTGCGATGGCATACCGTGCAGGTTGCCGTGTTGCCAATATG
GAATTTAACCAGTTCCATCCAACCTGTCTTTACCATCCGCAAGCACGTTCTTTCTTAATTACTGAAGCCATGCGTGGTGA
AGGTGCTTACTTACGTTTGCCAGATGGCGAACGTTTTATGCTACGTTTTGACGATCGTGCAGAGTTGGCTCCTCGCGATA
TTGTGGCACGTACCATTGACCATGAAATCAAGCGTCTCGGTATTCGCCATGTGTGGTTAGACATTACACACAAATCACCG
GATTTTATTAAAGAGCACTTCCCTACACTTTATGCACGATTGCTTGAGCTAGGTATCGACATCACTAAAGACATGATTCC
GGTGGTGCCGGCTGCACACTATACCTGTGGTGGTGTTGTGGTTGACTCAAATAGCCAGACTGATATTGAAGGTCTATATG
CAATTGGTGAAACCTCTTATACGGGTTTACATGGTGCAAACCGTATGGCGAGTAACTCATTACTTGAATGCTTCGTTTAT
GGCATGAGTGCAGCAAAAGATATCGAAAGTAAGTTTGATAAAAACTTCAAATTACCGGAAGTACCCACTTGGGACGATTC
CCAAGTAACCAATCCAGATGAAGATGTCGTGATTTTACAAAACTGGGATGAGTTACGCTCGACTATGTGGAACTACGTTG
GTATTGTAAGAACCACAAAACGTTTAGAACGTGCTTTACACCGTATTGAAATGCTAAAGCGCGAAATTACTGAATATTAT
CAAGATTATCGTGTCAGCAAAAACCTGATTGAACTGCGTAATCTTGTACTTGTTTCTGAAATGATTGTTCGTTGTGCTAT
GCACCGTAAAGAATCACGTGGCTTACATTACACACTTGATTATCCAAAAACTTCGAGTGAGATTCGTAAAACGGTTCTGA
CGCCTCCAAACTTTGCAGTAGAGCAACCTTTGGTGAATACCAGTGTATAA

Upstream 100 bases:

>100_bases
GCAATTTTTTAAGCTAATTGCAATTTTTCTGTGACTTAGAGCGCTTAAGCTCTTGCCTTTTTAGGTAAAAAGGGGTGTCA
TTAGCGGACGTTTTAAATTG

Downstream 100 bases:

>100_bases
ATAGTTTTTTATAAAAAAGCACGGTTCATGCCGTGCTTTTTTATTACTGAAGATTTATGGTCTAGGAGGAATTGAGTAAG
TCGCGGTCACATGTGCCACG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MRMDMPNQQTIHHFDVIIVGSGGAGLSLALSLPSHFNIAVLAKAALTEASTFYAQGGVAAVLDETDSIQQHINDTMIAGA
HLCEMDAVQHTVEGGRPSVDFLLKQGVQFTLDEDEQLHLTREGGHSQRRIIHSADATGKAISTTLVERAKERKNITIFEN
YIAIDLITSHKLGHTDQANRAIGLYALDESTEKVHTFLAPFTALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANM
EFNQFHPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDDRAELAPRDIVARTIDHEIKRLGIRHVWLDITHKSP
DFIKEHFPTLYARLLELGIDITKDMIPVVPAAHYTCGGVVVDSNSQTDIEGLYAIGETSYTGLHGANRMASNSLLECFVY
GMSAAKDIESKFDKNFKLPEVPTWDDSQVTNPDEDVVILQNWDELRSTMWNYVGIVRTTKRLERALHRIEMLKREITEYY
QDYRVSKNLIELRNLVLVSEMIVRCAMHRKESRGLHYTLDYPKTSSEIRKTVLTPPNFAVEQPLVNTSV

Sequences:

>Translated_549_residues
MRMDMPNQQTIHHFDVIIVGSGGAGLSLALSLPSHFNIAVLAKAALTEASTFYAQGGVAAVLDETDSIQQHINDTMIAGA
HLCEMDAVQHTVEGGRPSVDFLLKQGVQFTLDEDEQLHLTREGGHSQRRIIHSADATGKAISTTLVERAKERKNITIFEN
YIAIDLITSHKLGHTDQANRAIGLYALDESTEKVHTFLAPFTALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANM
EFNQFHPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDDRAELAPRDIVARTIDHEIKRLGIRHVWLDITHKSP
DFIKEHFPTLYARLLELGIDITKDMIPVVPAAHYTCGGVVVDSNSQTDIEGLYAIGETSYTGLHGANRMASNSLLECFVY
GMSAAKDIESKFDKNFKLPEVPTWDDSQVTNPDEDVVILQNWDELRSTMWNYVGIVRTTKRLERALHRIEMLKREITEYY
QDYRVSKNLIELRNLVLVSEMIVRCAMHRKESRGLHYTLDYPKTSSEIRKTVLTPPNFAVEQPLVNTSV
>Mature_549_residues
MRMDMPNQQTIHHFDVIIVGSGGAGLSLALSLPSHFNIAVLAKAALTEASTFYAQGGVAAVLDETDSIQQHINDTMIAGA
HLCEMDAVQHTVEGGRPSVDFLLKQGVQFTLDEDEQLHLTREGGHSQRRIIHSADATGKAISTTLVERAKERKNITIFEN
YIAIDLITSHKLGHTDQANRAIGLYALDESTEKVHTFLAPFTALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANM
EFNQFHPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDDRAELAPRDIVARTIDHEIKRLGIRHVWLDITHKSP
DFIKEHFPTLYARLLELGIDITKDMIPVVPAAHYTCGGVVVDSNSQTDIEGLYAIGETSYTGLHGANRMASNSLLECFVY
GMSAAKDIESKFDKNFKLPEVPTWDDSQVTNPDEDVVILQNWDELRSTMWNYVGIVRTTKRLERALHRIEMLKREITEYY
QDYRVSKNLIELRNLVLVSEMIVRCAMHRKESRGLHYTLDYPKTSSEIRKTVLTPPNFAVEQPLVNTSV

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=527, Percent_Identity=31.8785578747628, Blast_Score=213, Evalue=5e-55,
Organism=Escherichia coli, GI1788928, Length=535, Percent_Identity=60.5607476635514, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=524, Percent_Identity=30.7251908396947, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1786942, Length=572, Percent_Identity=30.2447552447552, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17505833, Length=535, Percent_Identity=30.6542056074766, Blast_Score=212, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17550100, Length=530, Percent_Identity=30.188679245283, Blast_Score=210, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI71986328, Length=421, Percent_Identity=27.790973871734, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322416, Length=560, Percent_Identity=32.3214285714286, Blast_Score=219, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6322701, Length=564, Percent_Identity=31.2056737588652, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6320788, Length=487, Percent_Identity=26.2833675564682, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24663005, Length=567, Percent_Identity=31.0405643738977, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI17137288, Length=555, Percent_Identity=31.3513513513514, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24655642, Length=555, Percent_Identity=31.3513513513514, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24655647, Length=555, Percent_Identity=31.3513513513514, Blast_Score=207, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 61554; Mature: 61554

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMDMPNQQTIHHFDVIIVGSGGAGLSLALSLPSHFNIAVLAKAALTEASTFYAQGGVAA
CCCCCCCCCCEEEEEEEEEECCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCEEE
VLDETDSIQQHINDTMIAGAHLCEMDAVQHTVEGGRPSVDFLLKQGVQFTLDEDEQLHLT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCCEEEEE
REGGHSQRRIIHSADATGKAISTTLVERAKERKNITIFENYIAIDLITSHKLGHTDQANR
ECCCCCHHHEEECCCCCCHHHHHHHHHHHHHCCCEEEEECEEEEEEEECCCCCCCCCCCC
AIGLYALDESTEKVHTFLAPFTALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANM
EEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEHHHCCCEEECC
EFNQFHPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDDRAELAPRDIVARTID
CCCCCCCEEEECCCCCHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHH
HEIKRLGIRHVWLDITHKSPDFIKEHFPTLYARLLELGIDITKDMIPVVPAAHYTCGGVV
HHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEECCCEE
VDSNSQTDIEGLYAIGETSYTGLHGANRMASNSLLECFVYGMSAAKDIESKFDKNFKLPE
ECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VPTWDDSQVTNPDEDVVILQNWDELRSTMWNYVGIVRTTKRLERALHRIEMLKREITEYY
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDYRVSKNLIELRNLVLVSEMIVRCAMHRKESRGLHYTLDYPKTSSEIRKTVLTPPNFAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCHH
EQPLVNTSV
CCCCCCCCC
>Mature Secondary Structure
MRMDMPNQQTIHHFDVIIVGSGGAGLSLALSLPSHFNIAVLAKAALTEASTFYAQGGVAA
CCCCCCCCCCEEEEEEEEEECCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCEEE
VLDETDSIQQHINDTMIAGAHLCEMDAVQHTVEGGRPSVDFLLKQGVQFTLDEDEQLHLT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCCEEEEE
REGGHSQRRIIHSADATGKAISTTLVERAKERKNITIFENYIAIDLITSHKLGHTDQANR
ECCCCCHHHEEECCCCCCHHHHHHHHHHHHHCCCEEEEECEEEEEEEECCCCCCCCCCCC
AIGLYALDESTEKVHTFLAPFTALACGGAMKAYLYTSNPDIATGDGIAMAYRAGCRVANM
EEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEHHHCCCEEECC
EFNQFHPTCLYHPQARSFLITEAMRGEGAYLRLPDGERFMLRFDDRAELAPRDIVARTID
CCCCCCCEEEECCCCCHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHH
HEIKRLGIRHVWLDITHKSPDFIKEHFPTLYARLLELGIDITKDMIPVVPAAHYTCGGVV
HHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEECCCEE
VDSNSQTDIEGLYAIGETSYTGLHGANRMASNSLLECFVYGMSAAKDIESKFDKNFKLPE
ECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VPTWDDSQVTNPDEDVVILQNWDELRSTMWNYVGIVRTTKRLERALHRIEMLKREITEYY
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDYRVSKNLIELRNLVLVSEMIVRCAMHRKESRGLHYTLDYPKTSSEIRKTVLTPPNFAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCHH
EQPLVNTSV
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]