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The map label for this gene is dusB [H]

Identifier: 213158218

GI number: 213158218

Start: 3033944

End: 3034969

Strand: Reverse

Name: dusB [H]

Synonym: AB57_2937

Alternate gene names: 213158218

Gene position: 3034969-3033944 (Counterclockwise)

Preceding gene: 213158221

Following gene: 213158215

Centisome position: 74.93

GC content: 40.35

Gene sequence:

>1026_bases
ATGTATATTGGTCCCTATCAACTGTCAAATAATTTAATTGTTGCCCCTATGGCCGGTGTAACTGACCGTCCATTTAGAAC
GCTGTGCAAGTATTTCGGTGCGGGTCATGCAGTCAGTGAAATGATGACTGCTGACAAGACTTTACGTATGACTAAAAAAA
GTCTTTATCGTGCTAATTTTGATGGAGAGTTAGCTCCAATTTCTGCACAGATTGCTGGTTCCGATCCAGAACAGTTGGCT
GAAGCGGCTCGTTACCAAGTCGCAAATGGCGCGCAAATTGTTGATATCAATATGGGATGTCCTGCAAAGAAAGTATGTAA
TAAATTAGCGGGTTCAGCATTATTACAAGATGAAGATTTGGTAGCTCGAATATTAGATGCAGTCGTTGCAGCAGTTGATG
TTCCGGTAACTTTAAAAACCCGTTTGGGATTTTTAAATGGCCAAGAGAATATTCTACGGGTGGCTAAGCGTGCCGAAGAG
GCAGGCATCGCTGCATTAGCACTACACGGCCGTACGCGTGAAGATATGTATTTAAATACCGCCCGTTATGAATTGATTAA
GCATGTTAAAGAATTAATCCATATTCCTGTGATTGCTAATGGTGATATTGATAGTCCTGAAAAAGCAAAATATGTACTGG
ACTATACTGGGGCAGATGCAATTATGATTGGGCGGGCTGCACAAGGTCGACCGTGGATTTTTCGTGAAATTGCCCATTAC
TTAAAAACTGGTGAACACCTTGCTGCTCCGAATATTGAAGAAGTTAAAGAAGTGCTTTTAGGGCATTTGTCTGAGTTATA
TCAGTTTTATGGTGAATACTCCGGTTGTCGTATTGCCCGTAAGCATATTGCTTGGTACACCAAAGGTTTACGCTCAAGTA
ATGAGTTTCGACAAAATATGTATAAAGTTGAAACGACTGCTGAACAAGCTTTAGTTGTTGAGAGCTATTTTAATCAATTG
CTTGCTGAAGGTAATTTGATGAGCGATGTTCAGGTTGAACAGGTTAATTTATTAGAAACTCACTAA

Upstream 100 bases:

>100_bases
GACAACGGTTTGTTCATAGAGAGGAGGGCACCTGCATAAAAAGATGAGCTGATCAAGCGGATGAAGTATACTATGCCGAT
CATCAATTGTATGAATATAT

Downstream 100 bases:

>100_bases
AATAAAGCAACCAGTACAGAGACTGGTTGCTTGGAATTTTTTATCTATTCACTGTCTAGCATTTCAACGACCAGTTGATT
AACCAGATCAGGTGCTTCAA

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MYIGPYQLSNNLIVAPMAGVTDRPFRTLCKYFGAGHAVSEMMTADKTLRMTKKSLYRANFDGELAPISAQIAGSDPEQLA
EAARYQVANGAQIVDINMGCPAKKVCNKLAGSALLQDEDLVARILDAVVAAVDVPVTLKTRLGFLNGQENILRVAKRAEE
AGIAALALHGRTREDMYLNTARYELIKHVKELIHIPVIANGDIDSPEKAKYVLDYTGADAIMIGRAAQGRPWIFREIAHY
LKTGEHLAAPNIEEVKEVLLGHLSELYQFYGEYSGCRIARKHIAWYTKGLRSSNEFRQNMYKVETTAEQALVVESYFNQL
LAEGNLMSDVQVEQVNLLETH

Sequences:

>Translated_341_residues
MYIGPYQLSNNLIVAPMAGVTDRPFRTLCKYFGAGHAVSEMMTADKTLRMTKKSLYRANFDGELAPISAQIAGSDPEQLA
EAARYQVANGAQIVDINMGCPAKKVCNKLAGSALLQDEDLVARILDAVVAAVDVPVTLKTRLGFLNGQENILRVAKRAEE
AGIAALALHGRTREDMYLNTARYELIKHVKELIHIPVIANGDIDSPEKAKYVLDYTGADAIMIGRAAQGRPWIFREIAHY
LKTGEHLAAPNIEEVKEVLLGHLSELYQFYGEYSGCRIARKHIAWYTKGLRSSNEFRQNMYKVETTAEQALVVESYFNQL
LAEGNLMSDVQVEQVNLLETH
>Mature_341_residues
MYIGPYQLSNNLIVAPMAGVTDRPFRTLCKYFGAGHAVSEMMTADKTLRMTKKSLYRANFDGELAPISAQIAGSDPEQLA
EAARYQVANGAQIVDINMGCPAKKVCNKLAGSALLQDEDLVARILDAVVAAVDVPVTLKTRLGFLNGQENILRVAKRAEE
AGIAALALHGRTREDMYLNTARYELIKHVKELIHIPVIANGDIDSPEKAKYVLDYTGADAIMIGRAAQGRPWIFREIAHY
LKTGEHLAAPNIEEVKEVLLGHLSELYQFYGEYSGCRIARKHIAWYTKGLRSSNEFRQNMYKVETTAEQALVVESYFNQL
LAEGNLMSDVQVEQVNLLETH

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI8923374, Length=298, Percent_Identity=30.8724832214765, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI239788483, Length=234, Percent_Identity=31.1965811965812, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI239788462, Length=235, Percent_Identity=31.063829787234, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=30.2222222222222, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=30.7017543859649, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=55.3125, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1788462, Length=238, Percent_Identity=31.0924369747899, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI145693211, Length=249, Percent_Identity=30.9236947791165, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=245, Percent_Identity=32.2448979591837, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=216, Percent_Identity=31.0185185185185, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=242, Percent_Identity=29.7520661157025, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=259, Percent_Identity=28.957528957529, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=234, Percent_Identity=32.4786324786325, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323433, Length=261, Percent_Identity=28.735632183908, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=225, Percent_Identity=26.2222222222222, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24585320, Length=292, Percent_Identity=28.7671232876712, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24580595, Length=271, Percent_Identity=30.9963099630996, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI19920448, Length=271, Percent_Identity=30.9963099630996, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=31.4285714285714, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=29.1304347826087, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37846; Mature: 37846

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIGPYQLSNNLIVAPMAGVTDRPFRTLCKYFGAGHAVSEMMTADKTLRMTKKSLYRANF
CCCCCEEECCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DGELAPISAQIAGSDPEQLAEAARYQVANGAQIVDINMGCPAKKVCNKLAGSALLQDEDL
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHH
VARILDAVVAAVDVPVTLKTRLGFLNGQENILRVAKRAEEAGIAALALHGRTREDMYLNT
HHHHHHHHHHHHCCCEEHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
ARYELIKHVKELIHIPVIANGDIDSPEKAKYVLDYTGADAIMIGRAAQGRPWIFREIAHY
HHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
LKTGEHLAAPNIEEVKEVLLGHLSELYQFYGEYSGCRIARKHIAWYTKGLRSSNEFRQNM
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
YKVETTAEQALVVESYFNQLLAEGNLMSDVQVEQVNLLETH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MYIGPYQLSNNLIVAPMAGVTDRPFRTLCKYFGAGHAVSEMMTADKTLRMTKKSLYRANF
CCCCCEEECCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DGELAPISAQIAGSDPEQLAEAARYQVANGAQIVDINMGCPAKKVCNKLAGSALLQDEDL
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHH
VARILDAVVAAVDVPVTLKTRLGFLNGQENILRVAKRAEEAGIAALALHGRTREDMYLNT
HHHHHHHHHHHHCCCEEHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
ARYELIKHVKELIHIPVIANGDIDSPEKAKYVLDYTGADAIMIGRAAQGRPWIFREIAHY
HHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
LKTGEHLAAPNIEEVKEVLLGHLSELYQFYGEYSGCRIARKHIAWYTKGLRSSNEFRQNM
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
YKVETTAEQALVVESYFNQLLAEGNLMSDVQVEQVNLLETH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]