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The map label for this gene is bacA [H]

Identifier: 213158108

GI number: 213158108

Start: 2904006

End: 2905853

Strand: Reverse

Name: bacA [H]

Synonym: AB57_2820

Alternate gene names: 213158108

Gene position: 2905853-2904006 (Counterclockwise)

Preceding gene: 213158109

Following gene: 213158106

Centisome position: 71.74

GC content: 38.8

Gene sequence:

>1848_bases
ATGCTAAAAGATTTGAGTCAAAATTTAGGGCAAATGAATGATAAAAATAACAATATAGAAAATTTCTTAAAGCAAATTTA
TGAAAATATTTTTGATCAAAATTCCAATCGAACAGCACTCATTTTTGAAGGTCGTAGCATTAGTTATGCTGAGGTAGGGG
CTCAGGTCGCCAAAGTCATGTATGCGCTAAAAATGCAAGACTTGGCGACAGGGTCGGTGGTGGCAATTTGCCTGAGAAAA
AGTCCTGAGCATATTTATACAGCTTTGGCATGTGCGCTTACTGGCATCATTTGGCTACCTGTAGATATGGATTCTCCGCC
GTCGCGACTGAACTATTTATTGACGAATAGTCGGGCAGATGTTGTGGTGAGTGACTCATTTATTGCAGGTGTGCAAACAC
TAAATATTAATGAGATTTTATCGGCTACAACTAAGTACAAACCGAGCTTTAATGCCGAAATTAACCGACGTCCGGCATAT
TATTTATATACTTCGGGAAGTACAGGGACTCCGAAATGTGTGGTCCTAAATAATCAGGCCACAGAAAACACGCTACAACA
GACGATCTCGGAATGGAAAATTACTACTGACGATGTGATTATGGCGGTAACACCGTTTCACCATGATATGTCGGTATTTG
ATGTATTTGCTTCAATGGCAGTGGGTGCAACTTTGGTGGTTCCATCTTTTGAGCAAAGTAAAAATGCTGTTGTCTGGGCC
AATTTAGTAGATCGTTGCAAGGTTACGATTTGGAGCAGTGTCCCTGCCATTGTCGATATGCTGTTCAGTGTAGCTCAAAA
AGAACAATTACAGTCTTTGCGTTTAATTGCCCAAGGCGGCGACTATATCAAGCCTTCACTTATAGCGAAGTTAAGACAAC
AATTACCTAACGCTCGTTTATTTTCATTAGGTGGGCCTACCGAGACCACAATTTGGAGTATATGGCATGAAATAAATGAG
CAAGATCAGGAAATTATTCCGTATGGGAAAGCTTTAGAAAATAACCGTTATTTTATTTCGGATGAAAATCTAAAGCCTTG
CCAAATGGGCGAGGTCGGTACGATGTATATGACTGGTCTGAATTTATCGAATGGTTATTTACTTGATGGGGAAATTAATT
ATAAAGATTTCGTTGATATTCAAGTGAGTGAAAATGAAACTCAAACTGCTTTTCGCATGAGTGATCAAGGGTATTTTAGG
GAAGATGGAAATATTATTTTTGCTGGACGTGAGGAAGGTTATTTAAAGATAAAAGGCGTGAGAATCTCTGCGGCCGAGAT
TGAAAATGCTTTGACGAAGAACCCGTATATCCATAACTGTGTGGTGATCAGTTGTGTACATCCAACGACTGAAACTCAAG
AGTTAGTCGCCGTTTATACTCTAGAAAATAACTACAAAACTACTCGTCTAAATAGCCCCGAATTAAAGAGTTTCTTAAAA
ATACATTTACCGAGTTCACATATTCCTTCGAAATATTTGGCCGTCGAAACCATACCTTTGACCCGAAATGGAAAAATTGA
TCGTAAAGCTGTGCAAGAAATCGCTCAGGGGAAAATTTACGTAACTTCTACGTTGTCTGCAAACTCTTCTAAAGCATCGT
CAGCAGTAGCTGAGGTGGGAGATTCGGTTTTATCGATTTTCCGTGAATGCATAGCTGACAGCCAAGCTGAAAAAATGGAT
ATTTTTTATAGAAGTGAAATATTAGCGATAGGCATTCGTCCGAAACAACTCATGAGTATTGCGCAGAAACTTTCTCAAAA
ACTCAATACACAAATTGATTTTTATACGCTAGTTAGCTGCAAAACAATTCAGGATGTGGTGAATAAAGTAGAGCAGCAAA
TAAAATAG

Upstream 100 bases:

>100_bases
TTATTCAAACAAACAGCGATAAAGACGACATTTTTATAACTTTGTTTTGAATATTAGGTCGGGTGGTATTGCATAAAGAA
TAGGGTGAGGACCAAGATAA

Downstream 100 bases:

>100_bases
AAAAATTGAAATGTGCTTAAAAGCGGAGTTGAGTTGTTTGCTTTTAAGCACATGGTTGGTTTTAGGGCAACTAGATTTGA
GCTAAGGTTGCCTTAATTTT

Product: non-ribosomal peptide synthetase

Products: NA

Alternate protein names: BA1; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; ATP-dependent cysteine adenylase; CysA; Cysteine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase; ATP-dependent glutamate adenylase; GluA; Glutamate activase; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; Glutamate racemase [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MLKDLSQNLGQMNDKNNNIENFLKQIYENIFDQNSNRTALIFEGRSISYAEVGAQVAKVMYALKMQDLATGSVVAICLRK
SPEHIYTALACALTGIIWLPVDMDSPPSRLNYLLTNSRADVVVSDSFIAGVQTLNINEILSATTKYKPSFNAEINRRPAY
YLYTSGSTGTPKCVVLNNQATENTLQQTISEWKITTDDVIMAVTPFHHDMSVFDVFASMAVGATLVVPSFEQSKNAVVWA
NLVDRCKVTIWSSVPAIVDMLFSVAQKEQLQSLRLIAQGGDYIKPSLIAKLRQQLPNARLFSLGGPTETTIWSIWHEINE
QDQEIIPYGKALENNRYFISDENLKPCQMGEVGTMYMTGLNLSNGYLLDGEINYKDFVDIQVSENETQTAFRMSDQGYFR
EDGNIIFAGREEGYLKIKGVRISAAEIENALTKNPYIHNCVVISCVHPTTETQELVAVYTLENNYKTTRLNSPELKSFLK
IHLPSSHIPSKYLAVETIPLTRNGKIDRKAVQEIAQGKIYVTSTLSANSSKASSAVAEVGDSVLSIFRECIADSQAEKMD
IFYRSEILAIGIRPKQLMSIAQKLSQKLNTQIDFYTLVSCKTIQDVVNKVEQQIK

Sequences:

>Translated_615_residues
MLKDLSQNLGQMNDKNNNIENFLKQIYENIFDQNSNRTALIFEGRSISYAEVGAQVAKVMYALKMQDLATGSVVAICLRK
SPEHIYTALACALTGIIWLPVDMDSPPSRLNYLLTNSRADVVVSDSFIAGVQTLNINEILSATTKYKPSFNAEINRRPAY
YLYTSGSTGTPKCVVLNNQATENTLQQTISEWKITTDDVIMAVTPFHHDMSVFDVFASMAVGATLVVPSFEQSKNAVVWA
NLVDRCKVTIWSSVPAIVDMLFSVAQKEQLQSLRLIAQGGDYIKPSLIAKLRQQLPNARLFSLGGPTETTIWSIWHEINE
QDQEIIPYGKALENNRYFISDENLKPCQMGEVGTMYMTGLNLSNGYLLDGEINYKDFVDIQVSENETQTAFRMSDQGYFR
EDGNIIFAGREEGYLKIKGVRISAAEIENALTKNPYIHNCVVISCVHPTTETQELVAVYTLENNYKTTRLNSPELKSFLK
IHLPSSHIPSKYLAVETIPLTRNGKIDRKAVQEIAQGKIYVTSTLSANSSKASSAVAEVGDSVLSIFRECIADSQAEKMD
IFYRSEILAIGIRPKQLMSIAQKLSQKLNTQIDFYTLVSCKTIQDVVNKVEQQIK
>Mature_615_residues
MLKDLSQNLGQMNDKNNNIENFLKQIYENIFDQNSNRTALIFEGRSISYAEVGAQVAKVMYALKMQDLATGSVVAICLRK
SPEHIYTALACALTGIIWLPVDMDSPPSRLNYLLTNSRADVVVSDSFIAGVQTLNINEILSATTKYKPSFNAEINRRPAY
YLYTSGSTGTPKCVVLNNQATENTLQQTISEWKITTDDVIMAVTPFHHDMSVFDVFASMAVGATLVVPSFEQSKNAVVWA
NLVDRCKVTIWSSVPAIVDMLFSVAQKEQLQSLRLIAQGGDYIKPSLIAKLRQQLPNARLFSLGGPTETTIWSIWHEINE
QDQEIIPYGKALENNRYFISDENLKPCQMGEVGTMYMTGLNLSNGYLLDGEINYKDFVDIQVSENETQTAFRMSDQGYFR
EDGNIIFAGREEGYLKIKGVRISAAEIENALTKNPYIHNCVVISCVHPTTETQELVAVYTLENNYKTTRLNSPELKSFLK
IHLPSSHIPSKYLAVETIPLTRNGKIDRKAVQEIAQGKIYVTSTLSANSSKASSAVAEVGDSVLSIFRECIADSQAEKMD
IFYRSEILAIGIRPKQLMSIAQKLSQKLNTQIDFYTLVSCKTIQDVVNKVEQQIK

Specific function: Activates five amino acids, incorporates two D-amino acids, releases and cyclizes the mature bacitracin [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=500, Percent_Identity=24.8, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI187761343, Length=500, Percent_Identity=24.8, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI45580730, Length=372, Percent_Identity=24.7311827956989, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI28416953, Length=621, Percent_Identity=23.1884057971014, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786801, Length=617, Percent_Identity=25.2836304700162, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1786810, Length=512, Percent_Identity=19.921875, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI145693145, Length=398, Percent_Identity=22.3618090452261, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556356, Length=560, Percent_Identity=24.4642857142857, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17550940, Length=586, Percent_Identity=19.9658703071672, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=413, Percent_Identity=24.455205811138, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32564422, Length=454, Percent_Identity=22.2466960352423, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI32564420, Length=454, Percent_Identity=22.2466960352423, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557194, Length=407, Percent_Identity=24.3243243243243, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71996755, Length=445, Percent_Identity=21.7977528089888, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538037, Length=376, Percent_Identity=22.3404255319149, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=26.1707988980716, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6319591, Length=444, Percent_Identity=21.8468468468468, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648676, Length=472, Percent_Identity=26.0593220338983, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24656500, Length=507, Percent_Identity=21.6962524654832, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24581924, Length=361, Percent_Identity=23.8227146814404, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356947, Length=502, Percent_Identity=20.9163346613546, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648260, Length=503, Percent_Identity=21.272365805169, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR013624
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF08415 NRPS; PF00550 PP-binding [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 68720; Mature: 68720

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKDLSQNLGQMNDKNNNIENFLKQIYENIFDQNSNRTALIFEGRSISYAEVGAQVAKVM
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
YALKMQDLATGSVVAICLRKSPEHIYTALACALTGIIWLPVDMDSPPSRLNYLLTNSRAD
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCEEEEEECCCCCHHHHEEEEECCCCC
VVVSDSFIAGVQTLNINEILSATTKYKPSFNAEINRRPAYYLYTSGSTGTPKCVVLNNQA
EEEECHHHHCEEEECHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCCCCEEEEECCCC
TENTLQQTISEWKITTDDVIMAVTPFHHDMSVFDVFASMAVGATLVVPSFEQSKNAVVWA
HHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEE
NLVDRCKVTIWSSVPAIVDMLFSVAQKEQLQSLRLIAQGGDYIKPSLIAKLRQQLPNARL
EHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEE
FSLGGPTETTIWSIWHEINEQDQEIIPYGKALENNRYFISDENLKPCQMGEVGTMYMTGL
EECCCCCHHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEEECCCCCCCCCCCCCEEEEEEE
NLSNGYLLDGEINYKDFVDIQVSENETQTAFRMSDQGYFREDGNIIFAGREEGYLKIKGV
ECCCCEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCEEECCCEEEEECCCCEEEEEEE
RISAAEIENALTKNPYIHNCVVISCVHPTTETQELVAVYTLENNYKTTRLNSPELKSFLK
EEEHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEEEEEEECCEEEECCCCHHHHHHHE
IHLPSSHIPSKYLAVETIPLTRNGKIDRKAVQEIAQGKIYVTSTLSANSSKASSAVAEVG
EECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHH
DSVLSIFRECIADSQAEKMDIFYRSEILAIGIRPKQLMSIAQKLSQKLNTQIDFYTLVSC
HHHHHHHHHHHCCCCCHHHEEEEECEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEH
KTIQDVVNKVEQQIK
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKDLSQNLGQMNDKNNNIENFLKQIYENIFDQNSNRTALIFEGRSISYAEVGAQVAKVM
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
YALKMQDLATGSVVAICLRKSPEHIYTALACALTGIIWLPVDMDSPPSRLNYLLTNSRAD
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCEEEEEECCCCCHHHHEEEEECCCCC
VVVSDSFIAGVQTLNINEILSATTKYKPSFNAEINRRPAYYLYTSGSTGTPKCVVLNNQA
EEEECHHHHCEEEECHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCCCCEEEEECCCC
TENTLQQTISEWKITTDDVIMAVTPFHHDMSVFDVFASMAVGATLVVPSFEQSKNAVVWA
HHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEE
NLVDRCKVTIWSSVPAIVDMLFSVAQKEQLQSLRLIAQGGDYIKPSLIAKLRQQLPNARL
EHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEE
FSLGGPTETTIWSIWHEINEQDQEIIPYGKALENNRYFISDENLKPCQMGEVGTMYMTGL
EECCCCCHHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEEECCCCCCCCCCCCCEEEEEEE
NLSNGYLLDGEINYKDFVDIQVSENETQTAFRMSDQGYFREDGNIIFAGREEGYLKIKGV
ECCCCEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCEEECCCEEEEECCCCEEEEEEE
RISAAEIENALTKNPYIHNCVVISCVHPTTETQELVAVYTLENNYKTTRLNSPELKSFLK
EEEHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEEEEEEECCEEEECCCCHHHHHHHE
IHLPSSHIPSKYLAVETIPLTRNGKIDRKAVQEIAQGKIYVTSTLSANSSKASSAVAEVG
EECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHH
DSVLSIFRECIADSQAEKMDIFYRSEILAIGIRPKQLMSIAQKLSQKLNTQIDFYTLVSC
HHHHHHHHHHHCCCCCHHHEEEEECEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEH
KTIQDVVNKVEQQIK
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9427658 [H]