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The map label for this gene is miaB [H]

Identifier: 213157982

GI number: 213157982

Start: 3591108

End: 3592559

Strand: Direct

Name: miaB [H]

Synonym: AB57_3477

Alternate gene names: 213157982

Gene position: 3591108-3592559 (Clockwise)

Preceding gene: 213157977

Following gene: 213157983

Centisome position: 88.66

GC content: 39.19

Gene sequence:

>1452_bases
ATGACGGTTCAAACCTTCATTCCCAATGGTGCCAAAGCTGCCTCAGAAAACACTGTTACCCAGCCAACGCACACCACTGA
TGTTTCAATAAAGAAACTATATATTGAAACTCAAGGGTGTCAGATGAATGAGTATGACAGTCACCGTATGGCAGACCTTT
TGGGGGATTCTCATGGTTATGTGTTAACGAATAACCCTAATGAAGCAGACATTCTGCTCATGAATACTTGCTCTATTCGT
GAAAAAGCACAAGAGAAAGTATTTAGTGAATTAGGTCGTTGGCGCAAATTAAAAGAACAAAATCCTGATCTTGTTATTGG
TGTGGGTGGATGTGTTGCTTCTCAAGAAGGTGACAATATTCAAAAACGTGCACCTTATGTCGACATGATTTTTGGTCCAC
AAACCTTACACCGTTTACCGCAAATGCTTGATCAACATCACGCACAAGTGGAAAAGCCGAAAAAAGAAAAAATTAAGCTC
GTTGATATTTCATTCCCTGATATTGAAAAATTCGACTTCTTACCTGAACCACGCGTAGAAGGTTTTAAAGCATTCGTTTC
TATTATGGAAGGTTGCTCTAAATACTGCTCTTTCTGTGTTGTTCCATACACTCGTGGTGAAGAAGTTTCACGTCCACTTG
ATGATGTTCTTGCAGAAATCGCAGGCCTTGCTGAAAAAGGTGTCCGTGAAATCAGCTTGCTTGGTCAAAATGTGAACGGA
TATCGTGGCGAAACATTCGAAGGTGGCATTTGTACCTTCCCTGAATTGTTACGACTCGTTGCAGAAATTCCAGGTATTGG
CCGTTTACGTTATACAACTTCACATCCACTTGAGTTCTCTGATGAACTTATTCAGTGTTATGAAGATTTACCTCAAATGG
TTTCACACTTACATTTGCCAGTACAAAGTGGATCTAACGATGTACTCAAAGCAATGAAGCGAAATCACACGATTGATGTA
TACATCGATAAGATTGCCAAGTTACGCAAAATACGCCCTGATATGCATTTATCGAGCGATTTTATCATTGGTTTCCCTGG
TGAAACAGATGAAAATTTTGCAGAAACATTGCAGTTTATCAAAGACTTAGACTTTGATCATTCTTATAGCTTTGTTTATT
CAAAACGCCCGGGTACCCCAGCATCTGACTTACCAGATACAACCCCTGAACACGTTAAAAAAGAACGTTTAGCACAAGTT
CAACAAGTCATTAAGCAATCTAGTATTGAAAAAACTGACGCAATGCTTGGCAAAATTGAACGTGTACTTATTGAAAAAGT
ATCTGATCAAGACCCTAATATTTTAGTAGGTACAGCAGACAATACACGTTTAGTGACTTTTGTAGGCGATGCATCGTGGG
TTGGTCGTTTTGCAGAGATTGAGATTACTGAAATTAAAACTTTAAATTTAGTTTACGGTGAACTCTTGAATCTTGAGCCT
GACGTGGCGTAA

Upstream 100 bases:

>100_bases
TTATTGTTATCTGTTCTGCTCAAAATACTGCTATAATGATTGGTTTGACCCGTTTTGGGTCATTTTATTATTTAACTAGA
TCGATCTGTTAGGAGCCTGC

Downstream 100 bases:

>100_bases
TGTAGAGGTATTCTAAAGTCTACTGAAAGGTTTTCTCTTGACTGCAGCGATTCGACGTACCGTAACATTTCCTGAAATTT
CAATGGAACGTTTAAAGAGC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTVQTFIPNGAKAASENTVTQPTHTTDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGYVLTNNPNEADILLMNTCSIR
EKAQEKVFSELGRWRKLKEQNPDLVIGVGGCVASQEGDNIQKRAPYVDMIFGPQTLHRLPQMLDQHHAQVEKPKKEKIKL
VDISFPDIEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNG
YRGETFEGGICTFPELLRLVAEIPGIGRLRYTTSHPLEFSDELIQCYEDLPQMVSHLHLPVQSGSNDVLKAMKRNHTIDV
YIDKIAKLRKIRPDMHLSSDFIIGFPGETDENFAETLQFIKDLDFDHSYSFVYSKRPGTPASDLPDTTPEHVKKERLAQV
QQVIKQSSIEKTDAMLGKIERVLIEKVSDQDPNILVGTADNTRLVTFVGDASWVGRFAEIEITEIKTLNLVYGELLNLEP
DVA

Sequences:

>Translated_483_residues
MTVQTFIPNGAKAASENTVTQPTHTTDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGYVLTNNPNEADILLMNTCSIR
EKAQEKVFSELGRWRKLKEQNPDLVIGVGGCVASQEGDNIQKRAPYVDMIFGPQTLHRLPQMLDQHHAQVEKPKKEKIKL
VDISFPDIEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNG
YRGETFEGGICTFPELLRLVAEIPGIGRLRYTTSHPLEFSDELIQCYEDLPQMVSHLHLPVQSGSNDVLKAMKRNHTIDV
YIDKIAKLRKIRPDMHLSSDFIIGFPGETDENFAETLQFIKDLDFDHSYSFVYSKRPGTPASDLPDTTPEHVKKERLAQV
QQVIKQSSIEKTDAMLGKIERVLIEKVSDQDPNILVGTADNTRLVTFVGDASWVGRFAEIEITEIKTLNLVYGELLNLEP
DVA
>Mature_482_residues
TVQTFIPNGAKAASENTVTQPTHTTDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGYVLTNNPNEADILLMNTCSIRE
KAQEKVFSELGRWRKLKEQNPDLVIGVGGCVASQEGDNIQKRAPYVDMIFGPQTLHRLPQMLDQHHAQVEKPKKEKIKLV
DISFPDIEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNGY
RGETFEGGICTFPELLRLVAEIPGIGRLRYTTSHPLEFSDELIQCYEDLPQMVSHLHLPVQSGSNDVLKAMKRNHTIDVY
IDKIAKLRKIRPDMHLSSDFIIGFPGETDENFAETLQFIKDLDFDHSYSFVYSKRPGTPASDLPDTTPEHVKKERLAQVQ
QVIKQSSIEKTDAMLGKIERVLIEKVSDQDPNILVGTADNTRLVTFVGDASWVGRFAEIEITEIKTLNLVYGELLNLEPD
VA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=445, Percent_Identity=34.8314606741573, Blast_Score=271, Evalue=9e-73,
Organism=Homo sapiens, GI28872782, Length=445, Percent_Identity=34.8314606741573, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI93277076, Length=465, Percent_Identity=27.9569892473118, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1786882, Length=453, Percent_Identity=57.6158940397351, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI1787057, Length=403, Percent_Identity=31.2655086848635, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17553146, Length=468, Percent_Identity=31.8376068376068, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71996771, Length=326, Percent_Identity=28.2208588957055, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356207, Length=454, Percent_Identity=33.0396475770925, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI19922432, Length=468, Percent_Identity=27.991452991453, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 54303; Mature: 54171

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQTFIPNGAKAASENTVTQPTHTTDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGY
CCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCE
VLTNNPNEADILLMNTCSIREKAQEKVFSELGRWRKLKEQNPDLVIGVGGCVASQEGDNI
EEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCH
QKRAPYVDMIFGPQTLHRLPQMLDQHHAQVEKPKKEKIKLVDISFPDIEKFDFLPEPRVE
HHHCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCHHCEEEEECCCCCCHHHCCCCCCHHH
GFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNG
HHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YRGETFEGGICTFPELLRLVAEIPGIGRLRYTTSHPLEFSDELIQCYEDLPQMVSHLHLP
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
VQSGSNDVLKAMKRNHTIDVYIDKIAKLRKIRPDMHLSSDFIIGFPGETDENFAETLQFI
CCCCCHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHH
KDLDFDHSYSFVYSKRPGTPASDLPDTTPEHVKKERLAQVQQVIKQSSIEKTDAMLGKIE
HHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RVLIEKVSDQDPNILVGTADNTRLVTFVGDASWVGRFAEIEITEIKTLNLVYGELLNLEP
HHHHHHHCCCCCCEEEECCCCCEEEEEECCHHHHCCEEEEEEEEEEEHHHHHHHHHCCCC
DVA
CCC
>Mature Secondary Structure 
TVQTFIPNGAKAASENTVTQPTHTTDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGY
CEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCE
VLTNNPNEADILLMNTCSIREKAQEKVFSELGRWRKLKEQNPDLVIGVGGCVASQEGDNI
EEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCH
QKRAPYVDMIFGPQTLHRLPQMLDQHHAQVEKPKKEKIKLVDISFPDIEKFDFLPEPRVE
HHHCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCHHCEEEEECCCCCCHHHCCCCCCHHH
GFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNG
HHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YRGETFEGGICTFPELLRLVAEIPGIGRLRYTTSHPLEFSDELIQCYEDLPQMVSHLHLP
CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
VQSGSNDVLKAMKRNHTIDVYIDKIAKLRKIRPDMHLSSDFIIGFPGETDENFAETLQFI
CCCCCHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHH
KDLDFDHSYSFVYSKRPGTPASDLPDTTPEHVKKERLAQVQQVIKQSSIEKTDAMLGKIE
HHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RVLIEKVSDQDPNILVGTADNTRLVTFVGDASWVGRFAEIEITEIKTLNLVYGELLNLEP
HHHHHHHCCCCCCEEEECCCCCEEEEEECCHHHHCCEEEEEEEEEEEHHHHHHHHHCCCC
DVA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA